Amino acid dipepetide frequency for bacterium HR21

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.889AlaAla: 11.889 ± 0.19
0.973AlaCys: 0.973 ± 0.037
4.112AlaAsp: 4.112 ± 0.088
7.954AlaGlu: 7.954 ± 0.135
3.77AlaPhe: 3.77 ± 0.087
7.569AlaGly: 7.569 ± 0.152
2.074AlaHis: 2.074 ± 0.064
3.981AlaIle: 3.981 ± 0.116
1.512AlaLys: 1.512 ± 0.06
14.101AlaLeu: 14.101 ± 0.248
1.388AlaMet: 1.388 ± 0.057
1.587AlaAsn: 1.587 ± 0.067
5.024AlaPro: 5.024 ± 0.112
4.575AlaGln: 4.575 ± 0.083
7.918AlaArg: 7.918 ± 0.13
4.219AlaSer: 4.219 ± 0.085
4.694AlaThr: 4.694 ± 0.117
8.377AlaVal: 8.377 ± 0.14
1.505AlaTrp: 1.505 ± 0.053
2.831AlaTyr: 2.831 ± 0.071
0.0AlaXaa: 0.0 ± 0.0
Cys
1.087CysAla: 1.087 ± 0.043
0.182CysCys: 0.182 ± 0.02
0.416CysAsp: 0.416 ± 0.027
0.475CysGlu: 0.475 ± 0.029
0.385CysPhe: 0.385 ± 0.026
1.042CysGly: 1.042 ± 0.049
0.268CysHis: 0.268 ± 0.023
0.397CysIle: 0.397 ± 0.026
0.098CysLys: 0.098 ± 0.013
0.979CysLeu: 0.979 ± 0.049
0.122CysMet: 0.122 ± 0.014
0.236CysAsn: 0.236 ± 0.022
0.654CysPro: 0.654 ± 0.04
0.421CysGln: 0.421 ± 0.028
0.984CysArg: 0.984 ± 0.044
0.636CysSer: 0.636 ± 0.034
0.538CysThr: 0.538 ± 0.033
0.753CysVal: 0.753 ± 0.039
0.217CysTrp: 0.217 ± 0.017
0.267CysTyr: 0.267 ± 0.021
0.0CysXaa: 0.0 ± 0.0
Asp
4.024AspAla: 4.024 ± 0.083
0.334AspCys: 0.334 ± 0.026
1.455AspAsp: 1.455 ± 0.06
2.353AspGlu: 2.353 ± 0.065
1.794AspPhe: 1.794 ± 0.053
3.476AspGly: 3.476 ± 0.097
0.655AspHis: 0.655 ± 0.033
2.325AspIle: 2.325 ± 0.072
0.621AspLys: 0.621 ± 0.033
3.173AspLeu: 3.173 ± 0.087
0.545AspMet: 0.545 ± 0.031
0.898AspAsn: 0.898 ± 0.061
2.693AspPro: 2.693 ± 0.075
0.844AspGln: 0.844 ± 0.038
2.535AspArg: 2.535 ± 0.076
2.6AspSer: 2.6 ± 0.103
3.101AspThr: 3.101 ± 0.095
3.085AspVal: 3.085 ± 0.074
0.569AspTrp: 0.569 ± 0.033
1.323AspTyr: 1.323 ± 0.051
0.0AspXaa: 0.0 ± 0.0
Glu
6.852GluAla: 6.852 ± 0.126
0.611GluCys: 0.611 ± 0.032
2.315GluAsp: 2.315 ± 0.064
5.808GluGlu: 5.808 ± 0.137
2.521GluPhe: 2.521 ± 0.066
4.184GluGly: 4.184 ± 0.088
1.935GluHis: 1.935 ± 0.077
2.692GluIle: 2.692 ± 0.073
1.326GluLys: 1.326 ± 0.05
10.667GluLeu: 10.667 ± 0.174
1.028GluMet: 1.028 ± 0.042
1.243GluAsn: 1.243 ± 0.048
3.73GluPro: 3.73 ± 0.075
3.846GluGln: 3.846 ± 0.097
7.371GluArg: 7.371 ± 0.146
2.509GluSer: 2.509 ± 0.068
2.573GluThr: 2.573 ± 0.082
4.878GluVal: 4.878 ± 0.101
1.071GluTrp: 1.071 ± 0.047
1.931GluTyr: 1.931 ± 0.065
0.0GluXaa: 0.0 ± 0.0
Phe
3.909PheAla: 3.909 ± 0.086
0.5PheCys: 0.5 ± 0.03
1.421PheAsp: 1.421 ± 0.051
1.962PheGlu: 1.962 ± 0.059
1.582PhePhe: 1.582 ± 0.059
3.159PheGly: 3.159 ± 0.081
0.853PheHis: 0.853 ± 0.045
1.164PheIle: 1.164 ± 0.048
0.428PheLys: 0.428 ± 0.028
4.657PheLeu: 4.657 ± 0.102
0.404PheMet: 0.404 ± 0.027
0.705PheAsn: 0.705 ± 0.039
2.788PhePro: 2.788 ± 0.077
1.366PheGln: 1.366 ± 0.053
3.648PheArg: 3.648 ± 0.084
2.045PheSer: 2.045 ± 0.064
1.804PheThr: 1.804 ± 0.07
3.024PheVal: 3.024 ± 0.072
0.899PheTrp: 0.899 ± 0.042
1.152PheTyr: 1.152 ± 0.049
0.0PheXaa: 0.0 ± 0.0
Gly
7.019GlyAla: 7.019 ± 0.122
0.839GlyCys: 0.839 ± 0.044
3.14GlyAsp: 3.14 ± 0.092
4.929GlyGlu: 4.929 ± 0.101
3.192GlyPhe: 3.192 ± 0.089
5.908GlyGly: 5.908 ± 0.151
1.529GlyHis: 1.529 ± 0.051
5.44GlyIle: 5.44 ± 0.106
2.439GlyLys: 2.439 ± 0.057
7.155GlyLeu: 7.155 ± 0.123
1.907GlyMet: 1.907 ± 0.061
2.028GlyAsn: 2.028 ± 0.127
2.112GlyPro: 2.112 ± 0.06
3.08GlyGln: 3.08 ± 0.088
5.493GlyArg: 5.493 ± 0.117
4.632GlySer: 4.632 ± 0.11
5.302GlyThr: 5.302 ± 0.172
6.491GlyVal: 6.491 ± 0.13
1.56GlyTrp: 1.56 ± 0.066
2.668GlyTyr: 2.668 ± 0.068
0.0GlyXaa: 0.0 ± 0.0
His
2.241HisAla: 2.241 ± 0.064
0.289HisCys: 0.289 ± 0.025
0.777HisAsp: 0.777 ± 0.035
1.228HisGlu: 1.228 ± 0.052
0.915HisPhe: 0.915 ± 0.047
1.804HisGly: 1.804 ± 0.051
0.672HisHis: 0.672 ± 0.039
1.047HisIle: 1.047 ± 0.044
0.304HisLys: 0.304 ± 0.025
2.313HisLeu: 2.313 ± 0.061
0.277HisMet: 0.277 ± 0.023
0.478HisAsn: 0.478 ± 0.028
1.839HisPro: 1.839 ± 0.063
0.846HisGln: 0.846 ± 0.042
2.239HisArg: 2.239 ± 0.089
1.054HisSer: 1.054 ± 0.044
1.025HisThr: 1.025 ± 0.041
1.677HisVal: 1.677 ± 0.06
0.401HisTrp: 0.401 ± 0.028
0.685HisTyr: 0.685 ± 0.038
0.0HisXaa: 0.0 ± 0.0
Ile
5.763IleAla: 5.763 ± 0.122
0.442IleCys: 0.442 ± 0.028
1.792IleAsp: 1.792 ± 0.066
2.9IleGlu: 2.9 ± 0.091
1.309IlePhe: 1.309 ± 0.057
3.859IleGly: 3.859 ± 0.094
0.81IleHis: 0.81 ± 0.037
1.483IleIle: 1.483 ± 0.053
0.585IleLys: 0.585 ± 0.034
4.427IleLeu: 4.427 ± 0.1
0.447IleMet: 0.447 ± 0.032
0.839IleAsn: 0.839 ± 0.042
3.295IlePro: 3.295 ± 0.082
1.221IleGln: 1.221 ± 0.049
3.744IleArg: 3.744 ± 0.09
2.005IleSer: 2.005 ± 0.061
2.306IleThr: 2.306 ± 0.085
4.069IleVal: 4.069 ± 0.102
0.526IleTrp: 0.526 ± 0.03
1.035IleTyr: 1.035 ± 0.049
0.0IleXaa: 0.0 ± 0.0
Lys
1.914LysAla: 1.914 ± 0.072
0.12LysCys: 0.12 ± 0.014
0.616LysAsp: 0.616 ± 0.036
1.152LysGlu: 1.152 ± 0.048
0.499LysPhe: 0.499 ± 0.032
1.209LysGly: 1.209 ± 0.046
0.439LysHis: 0.439 ± 0.028
0.789LysIle: 0.789 ± 0.042
0.513LysLys: 0.513 ± 0.038
2.404LysLeu: 2.404 ± 0.068
0.272LysMet: 0.272 ± 0.021
0.397LysAsn: 0.397 ± 0.027
1.424LysPro: 1.424 ± 0.053
0.774LysGln: 0.774 ± 0.042
1.728LysArg: 1.728 ± 0.067
0.793LysSer: 0.793 ± 0.036
1.003LysThr: 1.003 ± 0.04
1.419LysVal: 1.419 ± 0.05
0.201LysTrp: 0.201 ± 0.018
0.513LysTyr: 0.513 ± 0.033
0.0LysXaa: 0.0 ± 0.0
Leu
11.102LeuAla: 11.102 ± 0.188
1.541LeuCys: 1.541 ± 0.058
4.296LeuAsp: 4.296 ± 0.085
8.298LeuGlu: 8.298 ± 0.17
4.659LeuPhe: 4.659 ± 0.103
9.494LeuGly: 9.494 ± 0.166
2.941LeuHis: 2.941 ± 0.09
4.038LeuIle: 4.038 ± 0.095
2.064LeuLys: 2.064 ± 0.064
16.205LeuLeu: 16.205 ± 0.315
1.634LeuMet: 1.634 ± 0.052
2.205LeuAsn: 2.205 ± 0.078
8.457LeuPro: 8.457 ± 0.131
6.871LeuGln: 6.871 ± 0.135
13.257LeuArg: 13.257 ± 0.181
7.053LeuSer: 7.053 ± 0.12
5.526LeuThr: 5.526 ± 0.106
8.782LeuVal: 8.782 ± 0.128
2.719LeuTrp: 2.719 ± 0.087
3.496LeuTyr: 3.496 ± 0.094
0.0LeuXaa: 0.0 ± 0.0
Met
1.501MetAla: 1.501 ± 0.052
0.105MetCys: 0.105 ± 0.017
0.588MetAsp: 0.588 ± 0.03
1.132MetGlu: 1.132 ± 0.047
0.39MetPhe: 0.39 ± 0.024
1.219MetGly: 1.219 ± 0.051
0.308MetHis: 0.308 ± 0.025
0.445MetIle: 0.445 ± 0.029
0.353MetLys: 0.353 ± 0.025
2.043MetLeu: 2.043 ± 0.062
0.287MetMet: 0.287 ± 0.022
0.272MetAsn: 0.272 ± 0.023
1.19MetPro: 1.19 ± 0.039
0.643MetGln: 0.643 ± 0.033
1.512MetArg: 1.512 ± 0.048
0.913MetSer: 0.913 ± 0.035
0.784MetThr: 0.784 ± 0.034
1.266MetVal: 1.266 ± 0.052
0.179MetTrp: 0.179 ± 0.018
0.282MetTyr: 0.282 ± 0.021
0.0MetXaa: 0.0 ± 0.0
Asn
2.224AsnAla: 2.224 ± 0.081
0.181AsnCys: 0.181 ± 0.018
0.748AsnAsp: 0.748 ± 0.042
0.93AsnGlu: 0.93 ± 0.041
0.829AsnPhe: 0.829 ± 0.036
1.77AsnGly: 1.77 ± 0.083
0.311AsnHis: 0.311 ± 0.023
1.02AsnIle: 1.02 ± 0.059
0.325AsnLys: 0.325 ± 0.022
2.038AsnLeu: 2.038 ± 0.075
0.268AsnMet: 0.268 ± 0.02
0.497AsnAsn: 0.497 ± 0.041
2.024AsnPro: 2.024 ± 0.062
0.53AsnGln: 0.53 ± 0.036
1.627AsnArg: 1.627 ± 0.053
0.899AsnSer: 0.899 ± 0.048
1.23AsnThr: 1.23 ± 0.069
1.775AsnVal: 1.775 ± 0.062
0.325AsnTrp: 0.325 ± 0.025
0.564AsnTyr: 0.564 ± 0.035
0.0AsnXaa: 0.0 ± 0.0
Pro
5.736ProAla: 5.736 ± 0.125
0.55ProCys: 0.55 ± 0.034
2.97ProAsp: 2.97 ± 0.072
6.155ProGlu: 6.155 ± 0.113
2.162ProPhe: 2.162 ± 0.056
4.616ProGly: 4.616 ± 0.11
1.288ProHis: 1.288 ± 0.049
2.396ProIle: 2.396 ± 0.068
1.109ProLys: 1.109 ± 0.053
6.702ProLeu: 6.702 ± 0.116
1.106ProMet: 1.106 ± 0.049
1.331ProAsn: 1.331 ± 0.059
4.264ProPro: 4.264 ± 0.105
2.446ProGln: 2.446 ± 0.071
3.842ProArg: 3.842 ± 0.088
3.22ProSer: 3.22 ± 0.08
3.139ProThr: 3.139 ± 0.075
5.067ProVal: 5.067 ± 0.097
1.013ProTrp: 1.013 ± 0.043
1.692ProTyr: 1.692 ± 0.058
0.0ProXaa: 0.0 ± 0.0
Gln
3.981GlnAla: 3.981 ± 0.092
0.328GlnCys: 0.328 ± 0.026
1.254GlnAsp: 1.254 ± 0.045
2.977GlnGlu: 2.977 ± 0.077
1.049GlnPhe: 1.049 ± 0.047
2.75GlnGly: 2.75 ± 0.075
1.257GlnHis: 1.257 ± 0.058
1.433GlnIle: 1.433 ± 0.057
0.984GlnLys: 0.984 ± 0.04
6.634GlnLeu: 6.634 ± 0.131
0.805GlnMet: 0.805 ± 0.039
0.798GlnAsn: 0.798 ± 0.044
2.638GlnPro: 2.638 ± 0.078
3.067GlnGln: 3.067 ± 0.091
5.129GlnArg: 5.129 ± 0.109
1.833GlnSer: 1.833 ± 0.058
2.054GlnThr: 2.054 ± 0.057
2.635GlnVal: 2.635 ± 0.084
1.034GlnTrp: 1.034 ± 0.044
1.319GlnTyr: 1.319 ± 0.043
0.0GlnXaa: 0.0 ± 0.0
Arg
7.564ArgAla: 7.564 ± 0.142
0.898ArgCys: 0.898 ± 0.051
2.872ArgAsp: 2.872 ± 0.075
5.725ArgGlu: 5.725 ± 0.136
4.1ArgPhe: 4.1 ± 0.088
5.483ArgGly: 5.483 ± 0.101
2.136ArgHis: 2.136 ± 0.074
4.704ArgIle: 4.704 ± 0.1
1.794ArgLys: 1.794 ± 0.06
11.829ArgLeu: 11.829 ± 0.16
1.722ArgMet: 1.722 ± 0.053
1.808ArgAsn: 1.808 ± 0.059
3.938ArgPro: 3.938 ± 0.084
4.903ArgGln: 4.903 ± 0.121
8.905ArgArg: 8.905 ± 0.154
4.707ArgSer: 4.707 ± 0.108
4.138ArgThr: 4.138 ± 0.079
6.133ArgVal: 6.133 ± 0.126
2.625ArgTrp: 2.625 ± 0.081
3.139ArgTyr: 3.139 ± 0.085
0.0ArgXaa: 0.0 ± 0.0
Ser
5.369SerAla: 5.369 ± 0.111
0.49SerCys: 0.49 ± 0.027
1.993SerAsp: 1.993 ± 0.061
2.738SerGlu: 2.738 ± 0.066
2.224SerPhe: 2.224 ± 0.075
4.943SerGly: 4.943 ± 0.137
0.875SerHis: 0.875 ± 0.042
2.201SerIle: 2.201 ± 0.065
0.709SerLys: 0.709 ± 0.037
6.224SerLeu: 6.224 ± 0.103
0.758SerMet: 0.758 ± 0.04
0.985SerAsn: 0.985 ± 0.049
3.835SerPro: 3.835 ± 0.097
1.689SerGln: 1.689 ± 0.059
3.445SerArg: 3.445 ± 0.085
3.242SerSer: 3.242 ± 0.085
2.731SerThr: 2.731 ± 0.09
4.255SerVal: 4.255 ± 0.105
1.054SerTrp: 1.054 ± 0.044
1.383SerTyr: 1.383 ± 0.053
0.0SerXaa: 0.0 ± 0.0
Thr
5.863ThrAla: 5.863 ± 0.112
0.39ThrCys: 0.39 ± 0.025
2.181ThrAsp: 2.181 ± 0.078
3.049ThrGlu: 3.049 ± 0.063
1.751ThrPhe: 1.751 ± 0.064
4.415ThrGly: 4.415 ± 0.133
0.936ThrHis: 0.936 ± 0.043
2.191ThrIle: 2.191 ± 0.075
0.8ThrLys: 0.8 ± 0.036
7.096ThrLeu: 7.096 ± 0.13
0.607ThrMet: 0.607 ± 0.033
1.09ThrAsn: 1.09 ± 0.063
3.973ThrPro: 3.973 ± 0.088
1.591ThrGln: 1.591 ± 0.057
2.86ThrArg: 2.86 ± 0.063
2.255ThrSer: 2.255 ± 0.077
2.922ThrThr: 2.922 ± 0.101
5.201ThrVal: 5.201 ± 0.114
0.719ThrTrp: 0.719 ± 0.046
1.402ThrTyr: 1.402 ± 0.057
0.0ThrXaa: 0.0 ± 0.0
Val
7.531ValAla: 7.531 ± 0.114
0.815ValCys: 0.815 ± 0.034
3.103ValAsp: 3.103 ± 0.071
5.899ValGlu: 5.899 ± 0.104
2.767ValPhe: 2.767 ± 0.068
5.892ValGly: 5.892 ± 0.119
1.649ValHis: 1.649 ± 0.058
3.111ValIle: 3.111 ± 0.084
1.25ValLys: 1.25 ± 0.047
10.844ValLeu: 10.844 ± 0.18
1.085ValMet: 1.085 ± 0.045
1.498ValAsn: 1.498 ± 0.062
5.01ValPro: 5.01 ± 0.111
3.06ValGln: 3.06 ± 0.075
7.719ValArg: 7.719 ± 0.127
3.954ValSer: 3.954 ± 0.096
3.906ValThr: 3.906 ± 0.118
7.727ValVal: 7.727 ± 0.153
1.243ValTrp: 1.243 ± 0.056
2.054ValTyr: 2.054 ± 0.062
0.0ValXaa: 0.0 ± 0.0
Trp
1.458TrpAla: 1.458 ± 0.049
0.215TrpCys: 0.215 ± 0.019
0.867TrpAsp: 0.867 ± 0.04
1.563TrpGlu: 1.563 ± 0.058
0.513TrpPhe: 0.513 ± 0.027
1.409TrpGly: 1.409 ± 0.053
0.543TrpHis: 0.543 ± 0.03
0.805TrpIle: 0.805 ± 0.039
0.528TrpLys: 0.528 ± 0.031
2.494TrpLeu: 2.494 ± 0.077
0.447TrpMet: 0.447 ± 0.032
0.5TrpAsn: 0.5 ± 0.034
0.16TrpPro: 0.16 ± 0.016
1.13TrpGln: 1.13 ± 0.052
1.876TrpArg: 1.876 ± 0.064
1.12TrpSer: 1.12 ± 0.053
0.858TrpThr: 0.858 ± 0.041
1.305TrpVal: 1.305 ± 0.054
0.482TrpTrp: 0.482 ± 0.029
0.629TrpTyr: 0.629 ± 0.038
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.025TyrAla: 3.025 ± 0.076
0.353TyrCys: 0.353 ± 0.028
1.488TyrAsp: 1.488 ± 0.057
1.863TyrGlu: 1.863 ± 0.066
1.061TyrPhe: 1.061 ± 0.046
2.661TyrGly: 2.661 ± 0.07
0.631TyrHis: 0.631 ± 0.034
1.195TyrIle: 1.195 ± 0.051
0.439TyrLys: 0.439 ± 0.026
2.652TyrLeu: 2.652 ± 0.075
0.301TyrMet: 0.301 ± 0.025
0.719TyrAsn: 0.719 ± 0.036
1.756TyrPro: 1.756 ± 0.056
1.056TyrGln: 1.056 ± 0.046
3.292TyrArg: 3.292 ± 0.083
1.455TyrSer: 1.455 ± 0.055
1.658TyrThr: 1.658 ± 0.058
2.2TyrVal: 2.2 ± 0.062
0.561TyrTrp: 0.561 ± 0.032
0.93TyrTyr: 0.93 ± 0.043
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1558 proteins (581441 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski