Sulfurospirillum deleyianum (strain ATCC 51133 / DSM 6946 / 5175)
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2243 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D1AYY0|D1AYY0_SULD5 Sulfatase OS=Sulfurospirillum deleyianum (strain ATCC 51133 / DSM 6946 / 5175) OX=525898 GN=Sdel_0080 PE=4 SV=1
MM1 pKa = 7.61 NSSLYY6 pKa = 10.85 NGVSGIKK13 pKa = 9.35 TSQFYY18 pKa = 10.73 IDD20 pKa = 4.3 VIGNNISNVNTNGFKK35 pKa = 10.85 GSTPEE40 pKa = 3.86 VSSLFSSILVGTYY53 pKa = 9.85 NSYY56 pKa = 11.54 SNGVGMGAQSFNTALDD72 pKa = 3.55 MSQGILEE79 pKa = 4.34 NTDD82 pKa = 3.01 NTFDD86 pKa = 4.16 LALGSEE92 pKa = 4.56 GWFGVQGADD101 pKa = 2.8 GNTYY105 pKa = 7.16 YY106 pKa = 10.54 TRR108 pKa = 11.84 AGSFSLDD115 pKa = 3.27 AEE117 pKa = 4.7 GNLVDD122 pKa = 5.15 GDD124 pKa = 4.36 GNYY127 pKa = 11.0 LLATLGNNIATTTLDD142 pKa = 3.66 ADD144 pKa = 3.95 TLAKK148 pKa = 10.37 FGQYY152 pKa = 10.3 YY153 pKa = 10.92 NNGTASSVTPYY164 pKa = 10.94 AITEE168 pKa = 4.23 LNDD171 pKa = 3.11 VGLGTIGSQGIVNLPKK187 pKa = 9.51 TLYY190 pKa = 8.69 YY191 pKa = 10.09 PPVASSEE198 pKa = 3.93 ASYY201 pKa = 11.01 SANLDD206 pKa = 3.35 PTITTDD212 pKa = 5.41 AVTLDD217 pKa = 4.74 LNSADD222 pKa = 3.96 YY223 pKa = 9.79 PSSVLADD230 pKa = 3.74 TTSKK234 pKa = 9.67 TLTLNGTITNTITTQTPQKK253 pKa = 10.73 GDD255 pKa = 3.34 IVDD258 pKa = 3.77 VTLKK262 pKa = 10.66 DD263 pKa = 3.65 ASGKK267 pKa = 8.11 TLLLTATLDD276 pKa = 3.91 EE277 pKa = 4.21 NLEE280 pKa = 4.1 WSINDD285 pKa = 3.68 YY286 pKa = 11.07 DD287 pKa = 4.06 VSAYY291 pKa = 10.3 DD292 pKa = 3.64 LSSAFTLTSAKK303 pKa = 10.3 LQTEE307 pKa = 4.33 QEE309 pKa = 4.4 VATQEE314 pKa = 4.0 NFSTSIIGPDD324 pKa = 3.54 GDD326 pKa = 5.17 KK327 pKa = 11.03 DD328 pKa = 3.61 ILDD331 pKa = 3.47 MTFTKK336 pKa = 10.37 VIPQTSTEE344 pKa = 4.41 TTWNAVVNIYY354 pKa = 10.72 SFYY357 pKa = 9.86 EE358 pKa = 4.15 TYY360 pKa = 9.06 DD361 pKa = 3.14 TSKK364 pKa = 9.89 TYY366 pKa = 9.19 DD367 pKa = 3.13 TTQYY371 pKa = 11.17 YY372 pKa = 9.72 IDD374 pKa = 3.4 KK375 pKa = 8.97 TANKK379 pKa = 9.42 VYY381 pKa = 10.37 EE382 pKa = 4.26 IVDD385 pKa = 3.93 TQTGALTFNGNGSLASNTIPTINNGGTTLTLNLGTVGGYY424 pKa = 10.78 DD425 pKa = 3.68 GMVSNANIDD434 pKa = 3.53 KK435 pKa = 11.15 ANASSSNGTLEE446 pKa = 4.47 GFLTGYY452 pKa = 10.71 DD453 pKa = 3.02 VDD455 pKa = 4.15 TNGNVIASFSNGKK468 pKa = 9.52 SSSVAKK474 pKa = 9.78 IAVYY478 pKa = 10.2 HH479 pKa = 5.7 FQNDD483 pKa = 3.25 QGLMAEE489 pKa = 4.26 SSNLFSEE496 pKa = 4.69 SSNSGKK502 pKa = 10.57 AFFYY506 pKa = 10.52 ADD508 pKa = 3.4 EE509 pKa = 4.67 SGEE512 pKa = 4.2 SFLGSKK518 pKa = 10.17 VYY520 pKa = 9.49 SHH522 pKa = 7.12 KK523 pKa = 11.23 LEE525 pKa = 4.27 GSNVSLATALTEE537 pKa = 4.69 LIIVQKK543 pKa = 11.07 AFDD546 pKa = 4.07 ASAKK550 pKa = 10.62 SITTSDD556 pKa = 3.77 EE557 pKa = 3.98 LLKK560 pKa = 11.04 NAINMKK566 pKa = 10.28 AA567 pKa = 3.07
Molecular weight: 60.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.743
IPC2_protein 3.935
IPC_protein 3.961
Toseland 3.732
ProMoST 4.113
Dawson 3.948
Bjellqvist 4.088
Wikipedia 3.872
Rodwell 3.77
Grimsley 3.643
Solomon 3.935
Lehninger 3.897
Nozaki 4.05
DTASelect 4.304
Thurlkill 3.783
EMBOSS 3.884
Sillero 4.075
Patrickios 1.138
IPC_peptide 3.935
IPC2_peptide 4.05
IPC2.peptide.svr19 3.938
Protein with the highest isoelectric point:
>tr|D1B2Y5|D1B2Y5_SULD5 Uncharacterized protein OS=Sulfurospirillum deleyianum (strain ATCC 51133 / DSM 6946 / 5175) OX=525898 GN=Sdel_1437 PE=4 SV=1
MM1 pKa = 7.28 KK2 pKa = 9.39 RR3 pKa = 11.84 TYY5 pKa = 10.1 QPHH8 pKa = 4.92 TTPKK12 pKa = 10.24 KK13 pKa = 7.56 RR14 pKa = 11.84 THH16 pKa = 6.02 GFRR19 pKa = 11.84 LRR21 pKa = 11.84 MKK23 pKa = 9.27 TKK25 pKa = 10.31 GGRR28 pKa = 11.84 KK29 pKa = 9.04 VIAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.08 GRR39 pKa = 11.84 RR40 pKa = 11.84 RR41 pKa = 11.84 LAAA44 pKa = 4.25
Molecular weight: 5.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.414
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.384
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.106
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.021
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2243
0
2243
701989
32
4798
313.0
35.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.492 ± 0.056
1.07 ± 0.023
4.945 ± 0.061
7.338 ± 0.05
5.047 ± 0.044
6.174 ± 0.064
2.379 ± 0.029
7.654 ± 0.045
7.603 ± 0.061
10.438 ± 0.061
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.776 ± 0.029
4.431 ± 0.049
3.189 ± 0.036
3.172 ± 0.031
3.618 ± 0.035
6.556 ± 0.044
5.153 ± 0.051
6.457 ± 0.043
0.784 ± 0.019
3.723 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here