Pichia sorbitophila (strain ATCC MYA-4447 / BCRC 22081 / CBS 7064 / NBRC 10061 / NRRL Y-12695) (Hybrid yeast)
Average proteome isoelectric point is 6.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8851 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G8YQ21|G8YQ21_PICSO Piso0_000785 protein OS=Pichia sorbitophila (strain ATCC MYA-4447 / BCRC 22081 / CBS 7064 / NBRC 10061 / NRRL Y-12695) OX=559304 GN=Piso0_000785 PE=3 SV=1
MM1 pKa = 6.88 VAASKK6 pKa = 11.06 ALIVVVLVSTIQALLIDD23 pKa = 4.0 RR24 pKa = 11.84 TNDD27 pKa = 2.81 IEE29 pKa = 5.11 KK30 pKa = 10.35 RR31 pKa = 11.84 ALLSGITSLVSSVEE45 pKa = 3.99 DD46 pKa = 3.61 LLGGLLSAVEE56 pKa = 4.1 AQLEE60 pKa = 4.54 CIADD64 pKa = 3.74 VVLGDD69 pKa = 4.68 DD70 pKa = 4.63 GQITTANEE78 pKa = 4.1 DD79 pKa = 3.79 EE80 pKa = 4.43 FTLAVILANGFYY92 pKa = 10.3 DD93 pKa = 4.04 YY94 pKa = 11.66 GSLDD98 pKa = 3.67 SFSSLQSTLNSVADD112 pKa = 4.03 NDD114 pKa = 4.89 PGLLNNNTLANYY126 pKa = 9.86 LGVSITDD133 pKa = 3.35 NVQGVDD139 pKa = 3.34 NKK141 pKa = 11.24 ADD143 pKa = 3.47 VLTTFIKK150 pKa = 10.66 SVIEE154 pKa = 3.91 VLDD157 pKa = 4.83 DD158 pKa = 3.76 NLSSLGGTSVTSDD171 pKa = 3.07 TLANAIQEE179 pKa = 4.32 KK180 pKa = 10.69 AEE182 pKa = 3.86 QNGIDD187 pKa = 3.77 FSLSEE192 pKa = 4.58 DD193 pKa = 3.47 ASSDD197 pKa = 3.59 SVQLVITDD205 pKa = 3.99 SQWLCKK211 pKa = 10.54 LLDD214 pKa = 3.72 HH215 pKa = 6.89 VVDD218 pKa = 4.12 GLEE221 pKa = 4.02 GLVDD225 pKa = 4.09 CVSVTLTNLKK235 pKa = 10.02 SVYY238 pKa = 10.49 NLVDD242 pKa = 3.45 EE243 pKa = 4.85 VLGSLCPAASSSSSGVSSTAPSSTSGSSATSAPSGSSTPTSAATSGASGASSATGSGSPSGSSGTSSGSPSSSSTSGSGSPSGASSATGSGSPSGSSGTSSGSPSSSSTSGSGSPSGASSATSSGSPSGSSSATGSGSPSGSGTSGSPSGSGTSGSPSGSSASGASSVTSSGSPSGSSASGSGSSGASSATSSGSPSGASSATGSGASGSGSSSATASGSSSGSGASSVASASATGSGGSGSSGIIGDD491 pKa = 3.84 LVGSIEE497 pKa = 4.01 GLLGGLLSAVEE508 pKa = 4.1 AQLEE512 pKa = 4.54 CIADD516 pKa = 3.74 VVLGDD521 pKa = 4.68 DD522 pKa = 4.63 GQITTANEE530 pKa = 4.1 DD531 pKa = 3.79 EE532 pKa = 4.43 FTLAVILANGFYY544 pKa = 10.3 DD545 pKa = 4.04 YY546 pKa = 11.66 GSLDD550 pKa = 3.67 SFSSLQSTLNSVADD564 pKa = 4.03 NDD566 pKa = 4.89 PGLLNNNTLANYY578 pKa = 9.86 LGVSITDD585 pKa = 3.35 NVQGVDD591 pKa = 3.34 NKK593 pKa = 11.24 ADD595 pKa = 3.47 VLTTFIKK602 pKa = 10.68 SVIEE606 pKa = 3.85 VLADD610 pKa = 3.49 NLSSLGGTSVTSDD623 pKa = 3.07 TLANAIQEE631 pKa = 4.32 KK632 pKa = 10.69 AEE634 pKa = 3.86 QNGIDD639 pKa = 3.77 FSLSEE644 pKa = 4.58 DD645 pKa = 3.47 ASSDD649 pKa = 3.59 SVQLVITDD657 pKa = 3.99 SQWLCKK663 pKa = 10.2 LLNHH667 pKa = 5.99 VVDD670 pKa = 4.25 GLEE673 pKa = 3.96 GLVDD677 pKa = 4.09 CVSVTLTNLKK687 pKa = 10.02 SVYY690 pKa = 10.49 NLVDD694 pKa = 3.45 EE695 pKa = 4.85 VLGSLCPAASSSSSGVSSTAPSSTSGSSATSTPSGSSAPTSAATSGASGASSATGSGTPSGSSTSGSGSSSGASSATSSGSPSGSSASGSGASSATGSGTPSGSSASGSGSSSATGSGSPSGSSGTTSGSPSGSSSATGSGSPSGSSASGSGSASGASSATGSGASGSGSSSATGSGSPSGSGASGSGASSATGSGSPSSSSASGASSATSSGASSASSATGSPSGSGSSSATASGSSSGSGTSSVASASATGSGGSGSSGIIGDD960 pKa = 3.84 LVGSIEE966 pKa = 4.13 GLLSGLLSAVEE977 pKa = 4.51 SKK979 pKa = 10.93 LEE981 pKa = 4.38 CIADD985 pKa = 3.68 VVLGDD990 pKa = 4.15 NPQSTSATEE999 pKa = 3.83 DD1000 pKa = 3.54 EE1001 pKa = 5.0 YY1002 pKa = 11.24 ILAVILANGFYY1013 pKa = 10.28 DD1014 pKa = 4.16 YY1015 pKa = 11.21 RR1016 pKa = 11.84 SLDD1019 pKa = 3.59 SFDD1022 pKa = 4.64 SLQSTLNSVAEE1033 pKa = 4.19 NDD1035 pKa = 4.2 PDD1037 pKa = 4.05 LLNNNTLANYY1047 pKa = 9.86 LGVSITDD1054 pKa = 3.35 NVQGVDD1060 pKa = 3.34 NKK1062 pKa = 11.24 ADD1064 pKa = 3.47 VLTTFIKK1071 pKa = 10.68 SVIEE1075 pKa = 3.85 VLADD1079 pKa = 3.49 NLSSLGGTSVTSDD1092 pKa = 3.07 TLANAIQEE1100 pKa = 4.32 KK1101 pKa = 10.69 AEE1103 pKa = 3.86 QNGIDD1108 pKa = 3.77 FSLSEE1113 pKa = 4.58 DD1114 pKa = 3.47 ASSDD1118 pKa = 3.59 SVQLVITDD1126 pKa = 3.99 SQWLCKK1132 pKa = 10.2 LLNHH1136 pKa = 5.99 VVDD1139 pKa = 4.16 GLEE1142 pKa = 4.14 GLVQCTVVDD1151 pKa = 4.56 LFDD1154 pKa = 4.52 LKK1156 pKa = 10.93 SVYY1159 pKa = 10.87 NLVDD1163 pKa = 3.45 EE1164 pKa = 4.85 VLGSLCPTASSSSSAVSSTAPSSTSGSSATSAPSGSSTPTSAATSGASGASSASGSGSSGSGSPSGSGASSATSSGSTSGSGASGSGSSSATSSGSPSGSGASGASGSSSATGSATGSGSPSGSSGTSSGSPSGASSATGSGASGSGSSSATGSGSPSGSGASGSGSSSATSSGSPSGSSTSGSSASGASSATGSGSPSGTSSSSASGASSATSSGSPSGSGASGSGSSSATSSGSPSGSGASGSGSSSATSSGSSSGSGASGASGSSSATGSGSSSGSGASSATSTGSPSGSGSSGSGSSSATGSTTGSGSPSGSGASGSGSSSATSSGSPSGASSATGSGASGSGSSSATGSGSPSGSGASGSGSSSATSSGSPSGSGASSATSSGSTSGSGASGSGSSSATSSGSPSGSGASGASGSSSATGSATGSGSPSGSSGTSSGSPSGASSATGSGASGSGSSSATGSGSPSGSGASGSGSSSATSSGSPSGSSTSGSSASGASSATGSGSPSGTSSSSASGASSATSSGSPSGSGASGSGSSSATSSGSSSGSGASGASGSSSATGSGSSSGSGASSATSTGSPSGSGSSGSGSSSATGSTTGSGSPSGSGASGSGSSSATSSGSPSGSGASGASGSSSATGSATGSGSPSGSSGTSSGSPSGSSASGASSASGTGASGASGSSSATSSGSASGTGASGSGSSSATGSATGSGSASGSGASSVASASATGSGGSGSSGLIGDD1905 pKa = 4.63 LVGSIEE1911 pKa = 4.13 GLLSGLLSAVEE1922 pKa = 4.46 SKK1924 pKa = 10.98 LEE1926 pKa = 4.36 SIAEE1930 pKa = 4.18 VVLGDD1935 pKa = 3.49 NAQISSAIEE1944 pKa = 3.74 DD1945 pKa = 4.07 EE1946 pKa = 4.73 YY1947 pKa = 11.22 ILAVILANGFYY1958 pKa = 10.28 DD1959 pKa = 3.83 YY1960 pKa = 10.98 RR1961 pKa = 11.84 SSDD1964 pKa = 3.48 SFNSLQSTLNSVAEE1978 pKa = 4.19 NDD1980 pKa = 4.2 PDD1982 pKa = 4.05 LLNNNTLANYY1992 pKa = 9.73 LGVGITDD1999 pKa = 3.34 NVQGVDD2005 pKa = 3.31 NKK2007 pKa = 11.2 ADD2009 pKa = 3.54 VLATFIKK2016 pKa = 10.62 SVIEE2020 pKa = 3.85 VLADD2024 pKa = 3.49 NLSSLGGTSVTSDD2037 pKa = 3.07 TLANAIQEE2045 pKa = 4.32 KK2046 pKa = 10.69 AEE2048 pKa = 3.86 QNGIDD2053 pKa = 3.77 FSLSEE2058 pKa = 4.58 DD2059 pKa = 3.47 ASSDD2063 pKa = 3.64 SVQLVIKK2070 pKa = 9.9 DD2071 pKa = 4.0 PQWICQVLDD2080 pKa = 3.72 LVVDD2084 pKa = 4.13 GLEE2087 pKa = 4.37 GLVQYY2092 pKa = 11.11 DD2093 pKa = 3.67 FADD2096 pKa = 3.73 SFDD2099 pKa = 4.38 LKK2101 pKa = 10.2 TIYY2104 pKa = 10.97 NLVDD2108 pKa = 3.44 EE2109 pKa = 5.07 VLGSLCPAASSSSSGVSSTAPSSTSGSSATSAPSGSSAPTSAATSGASGASSATGSGTPSGSGASGSGSSSATGSGSGSSGSGSSGSGSSGSGSSSATGSGSPSGSGASGASGASSATGSGSPSGASSVAPASVSGVSKK2248 pKa = 9.52 GTNPTSTKK2256 pKa = 9.63 TEE2258 pKa = 3.96 YY2259 pKa = 10.41 SHH2261 pKa = 6.75 TVVTVTSCSGHH2272 pKa = 6.09 KK2273 pKa = 10.31 CSEE2276 pKa = 4.38 TPVTTGLTTVTKK2288 pKa = 10.58 DD2289 pKa = 2.98 STIYY2293 pKa = 8.01 TTYY2296 pKa = 10.65 CPLPTEE2302 pKa = 4.33 SAAPASSVAPASVTGVSKK2320 pKa = 9.64 GTNPTSTKK2328 pKa = 9.63 TEE2330 pKa = 3.96 YY2331 pKa = 10.41 SHH2333 pKa = 6.75 TVVTVTSCSGNKK2345 pKa = 9.78 CSEE2348 pKa = 4.39 TPVTTGLTTVTKK2360 pKa = 10.58 DD2361 pKa = 2.98 STIYY2365 pKa = 8.01 TTYY2368 pKa = 10.65 CPLPTEE2374 pKa = 4.55 SSAPAPVPSSKK2385 pKa = 10.5 VLEE2388 pKa = 4.45 SAAPASSVAPASVTGVSKK2406 pKa = 9.64 GTNPTSTKK2414 pKa = 9.63 TEE2416 pKa = 3.96 YY2417 pKa = 10.41 SHH2419 pKa = 6.75 TVVTVTSCSGNKK2431 pKa = 9.78 CSEE2434 pKa = 4.39 TPVTTGLTTVTKK2446 pKa = 10.58 DD2447 pKa = 2.98 STIYY2451 pKa = 8.01 TTYY2454 pKa = 10.65 CPLPTEE2460 pKa = 4.55 SSAPAPVPSSKK2471 pKa = 10.28 VPEE2474 pKa = 4.3 SAAPASSEE2482 pKa = 4.0 APASKK2487 pKa = 10.65 APASEE2492 pKa = 4.84 GPASQAAKK2500 pKa = 10.01 SSSGPAPSKK2509 pKa = 10.98 APASSEE2515 pKa = 4.1 APTSQAAEE2523 pKa = 4.19 SSSSPVAQSSSAAAPSVNSVSEE2545 pKa = 3.93 AGAIKK2550 pKa = 10.42 AGISMGPLFAGAVLLLLL2567 pKa = 4.63
Molecular weight: 233.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.688
IPC2_protein 3.732
IPC_protein 3.77
Toseland 3.541
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.681
Rodwell 3.592
Grimsley 3.452
Solomon 3.757
Lehninger 3.706
Nozaki 3.859
DTASelect 4.113
Thurlkill 3.592
EMBOSS 3.694
Sillero 3.884
Patrickios 1.113
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.792
Protein with the highest isoelectric point:
>tr|G8YHK2|G8YHK2_PICSO Piso0_003239 protein OS=Pichia sorbitophila (strain ATCC MYA-4447 / BCRC 22081 / CBS 7064 / NBRC 10061 / NRRL Y-12695) OX=559304 GN=Piso0_003239 PE=4 SV=1
MM1 pKa = 7.94 PSQKK5 pKa = 10.35 SFRR8 pKa = 11.84 VKK10 pKa = 10.47 QKK12 pKa = 9.98 LAKK15 pKa = 9.67 AQKK18 pKa = 9.21 QNRR21 pKa = 11.84 PLPQWIRR28 pKa = 11.84 LRR30 pKa = 11.84 TGNKK34 pKa = 8.39 IRR36 pKa = 11.84 YY37 pKa = 5.83 NAKK40 pKa = 8.24 RR41 pKa = 11.84 RR42 pKa = 11.84 HH43 pKa = 4.11 WRR45 pKa = 11.84 RR46 pKa = 11.84 TKK48 pKa = 11.12 LNII51 pKa = 3.83
Molecular weight: 6.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.391
IPC2_protein 11.052
IPC_protein 12.398
Toseland 12.559
ProMoST 13.042
Dawson 12.559
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.384
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.544
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.106
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 8.993
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8851
0
8851
4358694
40
4944
492.5
55.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.676 ± 0.023
1.186 ± 0.009
6.058 ± 0.016
6.694 ± 0.026
4.42 ± 0.018
5.381 ± 0.025
2.192 ± 0.01
6.35 ± 0.021
7.29 ± 0.027
9.321 ± 0.028
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.95 ± 0.01
5.756 ± 0.02
4.267 ± 0.021
3.641 ± 0.018
4.573 ± 0.016
9.786 ± 0.04
5.195 ± 0.014
5.729 ± 0.015
0.999 ± 0.007
3.537 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here