Galerina marginata (strain CBS 339.88)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetidae; Agaricales; Strophariaceae; Galerina; Galerina marginata

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 21357 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A067SQS3|A0A067SQS3_GALM3 Uncharacterized protein OS=Galerina marginata (strain CBS 339.88) OX=685588 GN=GALMADRAFT_145843 PE=4 SV=1
MM1 pKa = 7.88VLEE4 pKa = 5.41ADD6 pKa = 4.25TSPDD10 pKa = 3.15TCVDD14 pKa = 3.53SSRR17 pKa = 11.84ARR19 pKa = 11.84YY20 pKa = 8.23WYY22 pKa = 9.77YY23 pKa = 11.33SSAFTPGNVAVRR35 pKa = 11.84FTSDD39 pKa = 3.39LNDD42 pKa = 3.13VCGWMQDD49 pKa = 3.12LDD51 pKa = 3.74

Molecular weight: 5.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A067T1Q9|A0A067T1Q9_GALM3 AAA domain-containing protein OS=Galerina marginata (strain CBS 339.88) OX=685588 GN=GALMADRAFT_66085 PE=4 SV=1
MM1 pKa = 7.17SRR3 pKa = 11.84KK4 pKa = 9.29FFAVVNGSSLRR15 pKa = 11.84SHH17 pKa = 6.4HH18 pKa = 6.37HH19 pKa = 4.84RR20 pKa = 11.84HH21 pKa = 5.69HH22 pKa = 6.7RR23 pKa = 11.84RR24 pKa = 11.84HH25 pKa = 5.56LHH27 pKa = 5.24QSHH30 pKa = 5.97HH31 pKa = 5.21HH32 pKa = 6.28HH33 pKa = 6.83RR34 pKa = 11.84LHH36 pKa = 6.64HH37 pKa = 6.65RR38 pKa = 11.84PLQSRR43 pKa = 11.84HH44 pKa = 4.5RR45 pKa = 11.84HH46 pKa = 4.32HH47 pKa = 6.82HH48 pKa = 5.2RR49 pKa = 11.84HH50 pKa = 4.45HH51 pKa = 6.05HH52 pKa = 4.43RR53 pKa = 11.84HH54 pKa = 4.73RR55 pKa = 11.84RR56 pKa = 11.84RR57 pKa = 11.84RR58 pKa = 11.84SRR60 pKa = 11.84RR61 pKa = 11.84CRR63 pKa = 11.84HH64 pKa = 4.22RR65 pKa = 11.84HH66 pKa = 4.25RR67 pKa = 11.84RR68 pKa = 11.84QHH70 pKa = 5.76RR71 pKa = 11.84RR72 pKa = 11.84QHH74 pKa = 5.01RR75 pKa = 11.84HH76 pKa = 3.44QHH78 pKa = 4.24RR79 pKa = 11.84HH80 pKa = 3.22QHH82 pKa = 5.25RR83 pKa = 11.84RR84 pKa = 11.84QHH86 pKa = 5.19RR87 pKa = 11.84QRR89 pKa = 11.84HH90 pKa = 4.27RR91 pKa = 11.84QHH93 pKa = 6.86RR94 pKa = 11.84LEE96 pKa = 4.53TGHH99 pKa = 5.83QRR101 pKa = 11.84RR102 pKa = 11.84GHH104 pKa = 5.39RR105 pKa = 11.84PSRR108 pKa = 11.84RR109 pKa = 11.84CHH111 pKa = 4.27QQHH114 pKa = 6.34RR115 pKa = 11.84LSRR118 pKa = 11.84QCRR121 pKa = 11.84QQHH124 pKa = 4.53QLSRR128 pKa = 11.84QCRR131 pKa = 11.84RR132 pKa = 11.84RR133 pKa = 11.84WGLYY137 pKa = 8.74RR138 pKa = 11.84HH139 pKa = 5.39QQHH142 pKa = 6.49LLSRR146 pKa = 11.84RR147 pKa = 11.84HH148 pKa = 6.0RR149 pKa = 11.84RR150 pKa = 11.84CQCWW154 pKa = 2.7

Molecular weight: 20.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

21354

3

21357

8193245

35

4944

383.6

42.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.969 ± 0.016

1.359 ± 0.008

5.416 ± 0.014

5.571 ± 0.017

4.086 ± 0.012

6.331 ± 0.02

2.589 ± 0.008

5.207 ± 0.015

4.586 ± 0.014

9.569 ± 0.02

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.012 ± 0.006

3.76 ± 0.01

6.48 ± 0.02

3.74 ± 0.012

6.018 ± 0.017

8.906 ± 0.019

6.115 ± 0.013

6.167 ± 0.013

1.455 ± 0.007

2.665 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski