Coxiella endosymbiont of Amblyomma americanum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales; Coxiellaceae; Coxiella; unclassified Coxiella (in: Bacteria)

Average proteome isoelectric point is 8.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 536 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A8E9C1|A0A0A8E9C1_9COXI Phosphoribosylformylglycinamidine cyclo-ligase OS=Coxiella endosymbiont of Amblyomma americanum OX=325775 GN=purM PE=3 SV=1
MM1 pKa = 7.08FKK3 pKa = 10.57KK4 pKa = 10.22YY5 pKa = 10.68VCLLCGFVYY14 pKa = 10.39EE15 pKa = 4.85EE16 pKa = 3.99EE17 pKa = 4.44KK18 pKa = 10.83GWPEE22 pKa = 4.4DD23 pKa = 4.15GIAPGTKK30 pKa = 9.09WSDD33 pKa = 3.43VPGNWVCPEE42 pKa = 4.12CGAMKK47 pKa = 10.5ADD49 pKa = 3.77FQMMEE54 pKa = 4.08TT55 pKa = 4.12

Molecular weight: 6.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A8EDT7|A0A0A8EDT7_9COXI ATP-dependent Clp protease ATP-binding subunit ClpX OS=Coxiella endosymbiont of Amblyomma americanum OX=325775 GN=clpX PE=3 SV=1
MM1 pKa = 7.88PKK3 pKa = 10.58LKK5 pKa = 9.91TNRR8 pKa = 11.84GAAKK12 pKa = 9.79RR13 pKa = 11.84FKK15 pKa = 9.77ITGQGGIKK23 pKa = 9.85RR24 pKa = 11.84AVANHH29 pKa = 4.92NHH31 pKa = 6.46ILTKK35 pKa = 10.35KK36 pKa = 7.27SQKK39 pKa = 9.53RR40 pKa = 11.84KK41 pKa = 8.91RR42 pKa = 11.84RR43 pKa = 11.84LRR45 pKa = 11.84KK46 pKa = 9.39LRR48 pKa = 11.84PVIKK52 pKa = 10.66NNILAIEE59 pKa = 4.18RR60 pKa = 11.84MLKK63 pKa = 9.6II64 pKa = 4.15

Molecular weight: 7.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

536

0

536

178281

55

1618

332.6

37.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.439 ± 0.081

1.41 ± 0.038

4.303 ± 0.069

5.399 ± 0.105

4.437 ± 0.096

6.267 ± 0.094

2.38 ± 0.042

9.278 ± 0.093

7.099 ± 0.091

10.39 ± 0.092

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.321 ± 0.04

4.799 ± 0.073

3.646 ± 0.044

3.722 ± 0.051

5.461 ± 0.078

6.343 ± 0.069

5.257 ± 0.063

6.585 ± 0.068

1.02 ± 0.039

3.442 ± 0.056
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski