Amino acid dipepetide frequency for Entomoplasma freundtii

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.091AlaAla: 4.091 ± 0.165
0.441AlaCys: 0.441 ± 0.047
2.999AlaAsp: 2.999 ± 0.113
3.062AlaGlu: 3.062 ± 0.114
3.36AlaPhe: 3.36 ± 0.127
3.507AlaGly: 3.507 ± 0.181
1.168AlaHis: 1.168 ± 0.08
5.938AlaIle: 5.938 ± 0.177
6.005AlaLys: 6.005 ± 0.182
7.05AlaLeu: 7.05 ± 0.184
1.561AlaMet: 1.561 ± 0.092
4.285AlaAsn: 4.285 ± 0.147
1.99AlaPro: 1.99 ± 0.098
2.768AlaGln: 2.768 ± 0.129
2.272AlaArg: 2.272 ± 0.086
3.614AlaSer: 3.614 ± 0.119
4.313AlaThr: 4.313 ± 0.22
3.376AlaVal: 3.376 ± 0.135
0.707AlaTrp: 0.707 ± 0.058
2.165AlaTyr: 2.165 ± 0.093
0.0AlaXaa: 0.0 ± 0.0
Cys
0.361CysAla: 0.361 ± 0.04
0.052CysCys: 0.052 ± 0.013
0.266CysAsp: 0.266 ± 0.032
0.365CysGlu: 0.365 ± 0.04
0.389CysPhe: 0.389 ± 0.042
0.552CysGly: 0.552 ± 0.053
0.187CysHis: 0.187 ± 0.033
0.286CysIle: 0.286 ± 0.03
0.31CysLys: 0.31 ± 0.037
0.79CysLeu: 0.79 ± 0.064
0.087CysMet: 0.087 ± 0.02
0.258CysAsn: 0.258 ± 0.036
0.334CysPro: 0.334 ± 0.046
0.338CysGln: 0.338 ± 0.038
0.187CysArg: 0.187 ± 0.028
0.33CysSer: 0.33 ± 0.035
0.218CysThr: 0.218 ± 0.029
0.278CysVal: 0.278 ± 0.032
0.095CysTrp: 0.095 ± 0.02
0.254CysTyr: 0.254 ± 0.032
0.0CysXaa: 0.0 ± 0.0
Asp
2.661AspAla: 2.661 ± 0.141
0.31AspCys: 0.31 ± 0.037
2.677AspAsp: 2.677 ± 0.113
3.606AspGlu: 3.606 ± 0.121
3.543AspPhe: 3.543 ± 0.129
3.114AspGly: 3.114 ± 0.128
0.965AspHis: 0.965 ± 0.073
3.59AspIle: 3.59 ± 0.135
4.17AspLys: 4.17 ± 0.137
6.541AspLeu: 6.541 ± 0.22
0.902AspMet: 0.902 ± 0.055
2.979AspAsn: 2.979 ± 0.127
1.751AspPro: 1.751 ± 0.077
2.391AspGln: 2.391 ± 0.106
1.525AspArg: 1.525 ± 0.071
2.836AspSer: 2.836 ± 0.121
2.065AspThr: 2.065 ± 0.102
3.014AspVal: 3.014 ± 0.137
0.933AspTrp: 0.933 ± 0.07
2.891AspTyr: 2.891 ± 0.112
0.0AspXaa: 0.0 ± 0.0
Glu
4.865GluAla: 4.865 ± 0.157
0.25GluCys: 0.25 ± 0.027
2.8GluAsp: 2.8 ± 0.117
4.039GluGlu: 4.039 ± 0.155
2.391GluPhe: 2.391 ± 0.105
3.078GluGly: 3.078 ± 0.13
0.913GluHis: 0.913 ± 0.074
6.033GluIle: 6.033 ± 0.164
5.751GluLys: 5.751 ± 0.179
5.687GluLeu: 5.687 ± 0.183
1.632GluMet: 1.632 ± 0.086
4.023GluAsn: 4.023 ± 0.133
1.863GluPro: 1.863 ± 0.09
2.081GluGln: 2.081 ± 0.096
2.065GluArg: 2.065 ± 0.098
3.003GluSer: 3.003 ± 0.116
4.325GluThr: 4.325 ± 0.129
4.405GluVal: 4.405 ± 0.142
0.643GluTrp: 0.643 ± 0.054
1.704GluTyr: 1.704 ± 0.088
0.0GluXaa: 0.0 ± 0.0
Phe
3.447PheAla: 3.447 ± 0.117
0.381PheCys: 0.381 ± 0.043
2.832PheAsp: 2.832 ± 0.106
2.903PheGlu: 2.903 ± 0.125
2.617PhePhe: 2.617 ± 0.117
3.431PheGly: 3.431 ± 0.134
0.794PheHis: 0.794 ± 0.056
3.801PheIle: 3.801 ± 0.153
3.523PheLys: 3.523 ± 0.129
5.505PheLeu: 5.505 ± 0.183
1.013PheMet: 1.013 ± 0.067
3.034PheAsn: 3.034 ± 0.119
1.454PhePro: 1.454 ± 0.073
1.966PheGln: 1.966 ± 0.084
1.438PheArg: 1.438 ± 0.077
3.11PheSer: 3.11 ± 0.121
2.629PheThr: 2.629 ± 0.115
3.292PheVal: 3.292 ± 0.121
0.679PheTrp: 0.679 ± 0.065
1.823PheTyr: 1.823 ± 0.093
0.0PheXaa: 0.0 ± 0.0
Gly
4.019GlyAla: 4.019 ± 0.151
0.433GlyCys: 0.433 ± 0.047
2.967GlyAsp: 2.967 ± 0.113
3.134GlyGlu: 3.134 ± 0.132
3.324GlyPhe: 3.324 ± 0.132
3.531GlyGly: 3.531 ± 0.145
1.319GlyHis: 1.319 ± 0.079
4.79GlyIle: 4.79 ± 0.161
4.226GlyLys: 4.226 ± 0.142
6.768GlyLeu: 6.768 ± 0.187
1.462GlyMet: 1.462 ± 0.079
2.768GlyAsn: 2.768 ± 0.098
1.696GlyPro: 1.696 ± 0.092
2.327GlyGln: 2.327 ± 0.091
2.125GlyArg: 2.125 ± 0.101
3.451GlySer: 3.451 ± 0.157
3.384GlyThr: 3.384 ± 0.122
4.099GlyVal: 4.099 ± 0.161
0.786GlyTrp: 0.786 ± 0.066
2.546GlyTyr: 2.546 ± 0.09
0.0GlyXaa: 0.0 ± 0.0
His
0.906HisAla: 0.906 ± 0.056
0.135HisCys: 0.135 ± 0.024
1.096HisAsp: 1.096 ± 0.081
1.255HisGlu: 1.255 ± 0.077
0.993HisPhe: 0.993 ± 0.064
1.104HisGly: 1.104 ± 0.079
0.675HisHis: 0.675 ± 0.065
1.06HisIle: 1.06 ± 0.071
1.299HisLys: 1.299 ± 0.076
2.093HisLeu: 2.093 ± 0.111
0.246HisMet: 0.246 ± 0.03
1.088HisAsn: 1.088 ± 0.069
0.87HisPro: 0.87 ± 0.06
1.144HisGln: 1.144 ± 0.068
0.723HisArg: 0.723 ± 0.058
0.933HisSer: 0.933 ± 0.062
0.608HisThr: 0.608 ± 0.051
0.882HisVal: 0.882 ± 0.059
0.294HisTrp: 0.294 ± 0.036
0.842HisTyr: 0.842 ± 0.06
0.0HisXaa: 0.0 ± 0.0
Ile
5.783IleAla: 5.783 ± 0.156
0.62IleCys: 0.62 ± 0.049
4.77IleAsp: 4.77 ± 0.133
4.381IleGlu: 4.381 ± 0.137
3.999IlePhe: 3.999 ± 0.144
5.012IleGly: 5.012 ± 0.151
1.211IleHis: 1.211 ± 0.074
6.966IleIle: 6.966 ± 0.219
6.235IleLys: 6.235 ± 0.166
7.784IleLeu: 7.784 ± 0.215
1.759IleMet: 1.759 ± 0.086
5.243IleAsn: 5.243 ± 0.164
3.146IlePro: 3.146 ± 0.125
2.74IleGln: 2.74 ± 0.103
2.613IleArg: 2.613 ± 0.11
5.147IleSer: 5.147 ± 0.156
4.714IleThr: 4.714 ± 0.145
5.088IleVal: 5.088 ± 0.165
0.767IleTrp: 0.767 ± 0.051
2.788IleTyr: 2.788 ± 0.12
0.0IleXaa: 0.0 ± 0.0
Lys
5.401LysAla: 5.401 ± 0.174
0.31LysCys: 0.31 ± 0.036
4.234LysAsp: 4.234 ± 0.15
5.814LysGlu: 5.814 ± 0.191
2.907LysPhe: 2.907 ± 0.105
4.091LysGly: 4.091 ± 0.147
1.287LysHis: 1.287 ± 0.069
7.443LysIle: 7.443 ± 0.169
7.479LysLys: 7.479 ± 0.212
7.153LysLeu: 7.153 ± 0.182
2.593LysMet: 2.593 ± 0.104
6.212LysAsn: 6.212 ± 0.17
3.138LysPro: 3.138 ± 0.111
3.205LysGln: 3.205 ± 0.127
2.875LysArg: 2.875 ± 0.106
4.043LysSer: 4.043 ± 0.131
5.624LysThr: 5.624 ± 0.164
5.33LysVal: 5.33 ± 0.146
0.913LysTrp: 0.913 ± 0.064
2.593LysTyr: 2.593 ± 0.11
0.0LysXaa: 0.0 ± 0.0
Leu
7.876LeuAla: 7.876 ± 0.19
0.504LeuCys: 0.504 ± 0.047
5.711LeuAsp: 5.711 ± 0.163
6.772LeuGlu: 6.772 ± 0.216
4.683LeuPhe: 4.683 ± 0.172
6.386LeuGly: 6.386 ± 0.182
1.605LeuHis: 1.605 ± 0.096
8.44LeuIle: 8.44 ± 0.214
9.051LeuLys: 9.051 ± 0.201
9.413LeuLeu: 9.413 ± 0.195
2.486LeuMet: 2.486 ± 0.096
6.633LeuAsn: 6.633 ± 0.181
3.872LeuPro: 3.872 ± 0.134
3.439LeuGln: 3.439 ± 0.141
3.253LeuArg: 3.253 ± 0.116
6.057LeuSer: 6.057 ± 0.144
7.205LeuThr: 7.205 ± 0.215
7.216LeuVal: 7.216 ± 0.176
1.112LeuTrp: 1.112 ± 0.068
2.637LeuTyr: 2.637 ± 0.095
0.0LeuXaa: 0.0 ± 0.0
Met
2.057MetAla: 2.057 ± 0.101
0.075MetCys: 0.075 ± 0.017
1.084MetAsp: 1.084 ± 0.066
1.168MetGlu: 1.168 ± 0.068
0.834MetPhe: 0.834 ± 0.058
1.469MetGly: 1.469 ± 0.086
0.369MetHis: 0.369 ± 0.039
1.835MetIle: 1.835 ± 0.088
1.863MetLys: 1.863 ± 0.082
1.922MetLeu: 1.922 ± 0.093
0.604MetMet: 0.604 ± 0.045
1.327MetAsn: 1.327 ± 0.064
0.89MetPro: 0.89 ± 0.064
0.687MetGln: 0.687 ± 0.059
0.707MetArg: 0.707 ± 0.05
1.35MetSer: 1.35 ± 0.072
1.525MetThr: 1.525 ± 0.071
1.736MetVal: 1.736 ± 0.092
0.238MetTrp: 0.238 ± 0.032
0.512MetTyr: 0.512 ± 0.044
0.0MetXaa: 0.0 ± 0.0
Asn
3.098AsnAla: 3.098 ± 0.127
0.389AsnCys: 0.389 ± 0.039
3.535AsnAsp: 3.535 ± 0.13
3.864AsnGlu: 3.864 ± 0.112
3.404AsnPhe: 3.404 ± 0.119
3.523AsnGly: 3.523 ± 0.116
1.616AsnHis: 1.616 ± 0.077
4.337AsnIle: 4.337 ± 0.149
5.143AsnLys: 5.143 ± 0.148
7.276AsnLeu: 7.276 ± 0.206
1.076AsnMet: 1.076 ± 0.065
4.369AsnAsn: 4.369 ± 0.175
2.776AsnPro: 2.776 ± 0.103
3.245AsnGln: 3.245 ± 0.137
2.276AsnArg: 2.276 ± 0.098
3.304AsnSer: 3.304 ± 0.145
2.486AsnThr: 2.486 ± 0.113
3.38AsnVal: 3.38 ± 0.105
1.001AsnTrp: 1.001 ± 0.067
2.808AsnTyr: 2.808 ± 0.126
0.0AsnXaa: 0.0 ± 0.0
Pro
1.64ProAla: 1.64 ± 0.076
0.175ProCys: 0.175 ± 0.03
1.783ProAsp: 1.783 ± 0.085
2.74ProGlu: 2.74 ± 0.128
1.712ProPhe: 1.712 ± 0.085
2.145ProGly: 2.145 ± 0.09
0.85ProHis: 0.85 ± 0.055
2.923ProIle: 2.923 ± 0.12
2.919ProLys: 2.919 ± 0.127
4.023ProLeu: 4.023 ± 0.122
0.751ProMet: 0.751 ± 0.056
2.486ProAsn: 2.486 ± 0.109
0.814ProPro: 0.814 ± 0.061
1.553ProGln: 1.553 ± 0.07
1.076ProArg: 1.076 ± 0.066
2.24ProSer: 2.24 ± 0.099
2.3ProThr: 2.3 ± 0.104
1.807ProVal: 1.807 ± 0.074
0.552ProTrp: 0.552 ± 0.048
1.299ProTyr: 1.299 ± 0.08
0.0ProXaa: 0.0 ± 0.0
Gln
2.804GlnAla: 2.804 ± 0.109
0.159GlnCys: 0.159 ± 0.022
2.033GlnAsp: 2.033 ± 0.097
3.098GlnGlu: 3.098 ± 0.117
1.541GlnPhe: 1.541 ± 0.08
2.141GlnGly: 2.141 ± 0.125
0.496GlnHis: 0.496 ± 0.042
3.237GlnIle: 3.237 ± 0.106
4.508GlnLys: 4.508 ± 0.15
3.706GlnLeu: 3.706 ± 0.136
0.834GlnMet: 0.834 ± 0.054
2.975GlnAsn: 2.975 ± 0.138
1.525GlnPro: 1.525 ± 0.076
1.819GlnGln: 1.819 ± 0.106
1.485GlnArg: 1.485 ± 0.077
2.093GlnSer: 2.093 ± 0.081
2.574GlnThr: 2.574 ± 0.09
2.51GlnVal: 2.51 ± 0.1
0.477GlnTrp: 0.477 ± 0.047
1.1GlnTyr: 1.1 ± 0.078
0.0GlnXaa: 0.0 ± 0.0
Arg
1.763ArgAla: 1.763 ± 0.072
0.207ArgCys: 0.207 ± 0.029
1.791ArgAsp: 1.791 ± 0.078
2.327ArgGlu: 2.327 ± 0.091
1.803ArgPhe: 1.803 ± 0.076
1.922ArgGly: 1.922 ± 0.097
0.739ArgHis: 0.739 ± 0.048
2.296ArgIle: 2.296 ± 0.093
2.625ArgLys: 2.625 ± 0.123
3.848ArgLeu: 3.848 ± 0.131
0.659ArgMet: 0.659 ± 0.051
1.902ArgAsn: 1.902 ± 0.092
1.199ArgPro: 1.199 ± 0.079
1.855ArgGln: 1.855 ± 0.098
1.446ArgArg: 1.446 ± 0.081
1.791ArgSer: 1.791 ± 0.085
1.779ArgThr: 1.779 ± 0.086
1.831ArgVal: 1.831 ± 0.082
0.485ArgTrp: 0.485 ± 0.048
1.454ArgTyr: 1.454 ± 0.086
0.0ArgXaa: 0.0 ± 0.0
Ser
3.273SerAla: 3.273 ± 0.14
0.385SerCys: 0.385 ± 0.039
2.677SerAsp: 2.677 ± 0.112
3.364SerGlu: 3.364 ± 0.127
3.277SerPhe: 3.277 ± 0.134
3.729SerGly: 3.729 ± 0.114
1.076SerHis: 1.076 ± 0.066
3.952SerIle: 3.952 ± 0.14
4.134SerLys: 4.134 ± 0.127
7.042SerLeu: 7.042 ± 0.196
1.08SerMet: 1.08 ± 0.064
2.82SerAsn: 2.82 ± 0.11
2.137SerPro: 2.137 ± 0.088
2.828SerGln: 2.828 ± 0.121
2.097SerArg: 2.097 ± 0.092
3.487SerSer: 3.487 ± 0.145
3.153SerThr: 3.153 ± 0.125
3.082SerVal: 3.082 ± 0.149
0.886SerTrp: 0.886 ± 0.065
2.204SerTyr: 2.204 ± 0.088
0.0SerXaa: 0.0 ± 0.0
Thr
3.312ThrAla: 3.312 ± 0.135
0.298ThrCys: 0.298 ± 0.035
2.915ThrAsp: 2.915 ± 0.151
2.82ThrGlu: 2.82 ± 0.12
3.157ThrPhe: 3.157 ± 0.121
3.404ThrGly: 3.404 ± 0.12
1.045ThrHis: 1.045 ± 0.064
5.652ThrIle: 5.652 ± 0.222
5.163ThrLys: 5.163 ± 0.156
6.196ThrLeu: 6.196 ± 0.157
1.124ThrMet: 1.124 ± 0.052
4.333ThrAsn: 4.333 ± 0.154
2.546ThrPro: 2.546 ± 0.096
1.97ThrGln: 1.97 ± 0.099
1.748ThrArg: 1.748 ± 0.081
3.567ThrSer: 3.567 ± 0.143
3.813ThrThr: 3.813 ± 0.148
2.82ThrVal: 2.82 ± 0.131
0.711ThrTrp: 0.711 ± 0.069
2.077ThrTyr: 2.077 ± 0.092
0.0ThrXaa: 0.0 ± 0.0
Val
4.547ValAla: 4.547 ± 0.152
0.449ValCys: 0.449 ± 0.042
3.34ValAsp: 3.34 ± 0.136
3.713ValGlu: 3.713 ± 0.14
2.879ValPhe: 2.879 ± 0.136
4.043ValGly: 4.043 ± 0.164
0.878ValHis: 0.878 ± 0.059
5.199ValIle: 5.199 ± 0.159
4.706ValLys: 4.706 ± 0.153
5.997ValLeu: 5.997 ± 0.174
1.386ValMet: 1.386 ± 0.079
3.523ValAsn: 3.523 ± 0.18
2.24ValPro: 2.24 ± 0.097
1.851ValGln: 1.851 ± 0.072
1.926ValArg: 1.926 ± 0.085
3.574ValSer: 3.574 ± 0.118
3.741ValThr: 3.741 ± 0.249
4.393ValVal: 4.393 ± 0.155
0.711ValTrp: 0.711 ± 0.059
2.026ValTyr: 2.026 ± 0.095
0.0ValXaa: 0.0 ± 0.0
Trp
0.77TrpAla: 0.77 ± 0.066
0.052TrpCys: 0.052 ± 0.014
0.778TrpAsp: 0.778 ± 0.057
0.882TrpGlu: 0.882 ± 0.062
0.675TrpPhe: 0.675 ± 0.066
0.699TrpGly: 0.699 ± 0.06
0.222TrpHis: 0.222 ± 0.029
0.969TrpIle: 0.969 ± 0.073
1.029TrpLys: 1.029 ± 0.06
1.374TrpLeu: 1.374 ± 0.078
0.322TrpMet: 0.322 ± 0.04
0.806TrpAsn: 0.806 ± 0.062
0.369TrpPro: 0.369 ± 0.039
0.62TrpGln: 0.62 ± 0.056
0.334TrpArg: 0.334 ± 0.042
0.707TrpSer: 0.707 ± 0.05
0.798TrpThr: 0.798 ± 0.067
0.743TrpVal: 0.743 ± 0.063
0.262TrpTrp: 0.262 ± 0.039
0.405TrpTyr: 0.405 ± 0.038
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.994TyrAla: 1.994 ± 0.092
0.314TyrCys: 0.314 ± 0.039
2.176TyrAsp: 2.176 ± 0.097
2.244TyrGlu: 2.244 ± 0.093
2.184TyrPhe: 2.184 ± 0.1
2.272TyrGly: 2.272 ± 0.113
0.83TyrHis: 0.83 ± 0.066
2.022TyrIle: 2.022 ± 0.089
2.506TyrLys: 2.506 ± 0.104
4.182TyrLeu: 4.182 ± 0.129
0.671TyrMet: 0.671 ± 0.05
1.843TyrAsn: 1.843 ± 0.095
1.18TyrPro: 1.18 ± 0.069
2.292TyrGln: 2.292 ± 0.113
1.553TyrArg: 1.553 ± 0.095
2.045TyrSer: 2.045 ± 0.106
1.414TyrThr: 1.414 ± 0.071
1.843TyrVal: 1.843 ± 0.08
0.564TyrTrp: 0.564 ± 0.048
1.338TyrTyr: 1.338 ± 0.084
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 702 proteins (251788 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski