Stella humosa

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Stellaceae; Stella

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5422 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N1M9X5|A0A3N1M9X5_9PROT Uncharacterized protein OS=Stella humosa OX=94 GN=EDC65_2270 PE=4 SV=1
MM1 pKa = 7.28YY2 pKa = 8.3ATGNYY7 pKa = 9.48AIAVDD12 pKa = 4.96AYY14 pKa = 10.95NLYY17 pKa = 10.63APPPPPPPPPPPPPPSPHH35 pKa = 4.42QTINGQAGPDD45 pKa = 4.17SIDD48 pKa = 3.72GDD50 pKa = 3.71WGNDD54 pKa = 3.56TIRR57 pKa = 11.84GYY59 pKa = 10.76SGNDD63 pKa = 3.45TIRR66 pKa = 11.84GLDD69 pKa = 3.93GNDD72 pKa = 3.54KK73 pKa = 11.07LYY75 pKa = 11.24GDD77 pKa = 5.67DD78 pKa = 5.76GDD80 pKa = 5.81DD81 pKa = 4.49DD82 pKa = 5.21VNGNLGNDD90 pKa = 3.86LVHH93 pKa = 6.97GGPGQDD99 pKa = 3.74FVRR102 pKa = 11.84GGQGNDD108 pKa = 3.07QVFGQDD114 pKa = 2.91GDD116 pKa = 3.84DD117 pKa = 3.08WHH119 pKa = 7.87VNGNIGNDD127 pKa = 3.26SVYY130 pKa = 11.01GGTGNDD136 pKa = 3.77GVFGGPGQDD145 pKa = 2.99TLYY148 pKa = 11.37GEE150 pKa = 5.01AGNDD154 pKa = 3.59TLSGDD159 pKa = 4.21LEE161 pKa = 4.57NDD163 pKa = 3.25ALYY166 pKa = 10.63GGPGADD172 pKa = 3.21LFVINSNGGWDD183 pKa = 3.62VIGDD187 pKa = 4.01FNRR190 pKa = 11.84SQGDD194 pKa = 3.68KK195 pKa = 10.66IVIQADD201 pKa = 3.28INGTGVFTGQQVLAMVTTGGGGAALIDD228 pKa = 3.83FGGGHH233 pKa = 7.1KK234 pKa = 10.52LGILGVSPGQLRR246 pKa = 11.84ADD248 pKa = 4.49DD249 pKa = 4.06FLIMM253 pKa = 5.02

Molecular weight: 25.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N1KWV8|A0A3N1KWV8_9PROT Uncharacterized protein OS=Stella humosa OX=94 GN=EDC65_4792 PE=4 SV=1
MM1 pKa = 7.78RR2 pKa = 11.84RR3 pKa = 11.84RR4 pKa = 11.84PPRR7 pKa = 11.84WPRR10 pKa = 11.84PSPTTRR16 pKa = 11.84IRR18 pKa = 11.84IAVAASAASAARR30 pKa = 11.84KK31 pKa = 9.37ASSRR35 pKa = 11.84AILRR39 pKa = 11.84RR40 pKa = 11.84LRR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84ARR46 pKa = 11.84PSPSEE51 pKa = 3.63ARR53 pKa = 11.84TPSVPSARR61 pKa = 11.84AAQRR65 pKa = 11.84RR66 pKa = 11.84RR67 pKa = 11.84SASRR71 pKa = 11.84SRR73 pKa = 11.84SANRR77 pKa = 11.84PSPSPPRR84 pKa = 11.84ARR86 pKa = 11.84PSRR89 pKa = 11.84RR90 pKa = 11.84QPSAGRR96 pKa = 11.84NRR98 pKa = 11.84RR99 pKa = 11.84RR100 pKa = 11.84PSRR103 pKa = 11.84RR104 pKa = 11.84QHH106 pKa = 6.29RR107 pKa = 11.84SASRR111 pKa = 11.84SRR113 pKa = 11.84LPPGRR118 pKa = 11.84RR119 pKa = 11.84HH120 pKa = 6.01LPLRR124 pKa = 11.84PRR126 pKa = 11.84RR127 pKa = 11.84RR128 pKa = 11.84SGRR131 pKa = 11.84ASVKK135 pKa = 10.4DD136 pKa = 3.68GQSVKK141 pKa = 10.36DD142 pKa = 3.88GQSARR147 pKa = 11.84PGQSATRR154 pKa = 11.84PHH156 pKa = 6.72RR157 pKa = 11.84RR158 pKa = 11.84PPASARR164 pKa = 11.84HH165 pKa = 5.26SRR167 pKa = 11.84ATPAAGRR174 pKa = 11.84KK175 pKa = 8.3PNPNSSARR183 pKa = 11.84RR184 pKa = 11.84PDD186 pKa = 3.25RR187 pKa = 11.84AMLADD192 pKa = 3.93RR193 pKa = 11.84CRR195 pKa = 11.84SRR197 pKa = 11.84RR198 pKa = 11.84RR199 pKa = 11.84PPNARR204 pKa = 11.84RR205 pKa = 11.84NDD207 pKa = 3.54RR208 pKa = 11.84PRR210 pKa = 11.84RR211 pKa = 11.84RR212 pKa = 11.84HH213 pKa = 4.89PRR215 pKa = 11.84PRR217 pKa = 11.84CSVLANAMPVRR228 pKa = 11.84ASRR231 pKa = 11.84HH232 pKa = 4.43PRR234 pKa = 11.84ATGTRR239 pKa = 11.84ATAIGSRR246 pKa = 11.84HH247 pKa = 6.2PPTDD251 pKa = 2.78RR252 pKa = 11.84RR253 pKa = 11.84RR254 pKa = 11.84AGRR257 pKa = 11.84PSAARR262 pKa = 11.84ASRR265 pKa = 11.84TGEE268 pKa = 3.76ASPTARR274 pKa = 11.84ACRR277 pKa = 11.84PTPPSIRR284 pKa = 11.84PGRR287 pKa = 11.84ACHH290 pKa = 6.15RR291 pKa = 11.84PWWHH295 pKa = 6.95LARR298 pKa = 11.84PRR300 pKa = 11.84QTSCVHH306 pKa = 6.4AAWTMSAAAAPRR318 pKa = 11.84ARR320 pKa = 11.84KK321 pKa = 8.66AAAPP325 pKa = 3.78

Molecular weight: 36.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5422

0

5422

1801500

29

2835

332.3

35.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.034 ± 0.051

0.799 ± 0.01

5.831 ± 0.041

4.991 ± 0.03

3.533 ± 0.021

9.489 ± 0.05

2.024 ± 0.017

4.718 ± 0.021

2.194 ± 0.027

10.341 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.466 ± 0.015

2.084 ± 0.018

5.783 ± 0.03

2.851 ± 0.018

8.005 ± 0.04

4.447 ± 0.023

5.048 ± 0.021

7.849 ± 0.028

1.45 ± 0.014

2.063 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski