Sphingomonas sp. HDW15B

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2594 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G7YQG2|A0A6G7YQG2_9SPHN Maleylacetoacetate isomerase OS=Sphingomonas sp. HDW15B OX=2714943 GN=maiA PE=3 SV=1
MM1 pKa = 7.01QRR3 pKa = 11.84TVLDD7 pKa = 4.29LNSTTVSGSNPSISGIRR24 pKa = 11.84YY25 pKa = 8.13IAPDD29 pKa = 3.39QTGVAPNGKK38 pKa = 9.58QYY40 pKa = 10.01LTLDD44 pKa = 3.75GAVDD48 pKa = 3.32NLTRR52 pKa = 11.84TGGSWNVGGGDD63 pKa = 3.67GKK65 pKa = 9.29ITYY68 pKa = 10.33SFLEE72 pKa = 4.41KK73 pKa = 10.98GPGGQYY79 pKa = 10.81NNPHH83 pKa = 5.17EE84 pKa = 4.61TYY86 pKa = 10.79LAGLVGDD93 pKa = 4.7FSTFTAEE100 pKa = 3.51QRR102 pKa = 11.84AAARR106 pKa = 11.84DD107 pKa = 4.35SIALWDD113 pKa = 4.23DD114 pKa = 3.42VLDD117 pKa = 4.68AEE119 pKa = 4.92FKK121 pKa = 10.24EE122 pKa = 4.7SNGRR126 pKa = 11.84GADD129 pKa = 3.23ILFMNTGEE137 pKa = 4.54GGPGQAAAFIPDD149 pKa = 3.45YY150 pKa = 9.96QGKK153 pKa = 8.78YY154 pKa = 10.54GKK156 pKa = 9.58IEE158 pKa = 3.93GDD160 pKa = 3.68VYY162 pKa = 11.71VNAGQPDD169 pKa = 4.27NFDD172 pKa = 4.04LYY174 pKa = 11.29YY175 pKa = 10.93GGYY178 pKa = 9.09GQTALTHH185 pKa = 6.54EE186 pKa = 4.57IGHH189 pKa = 7.1AIGLSHH195 pKa = 6.82TGDD198 pKa = 3.79YY199 pKa = 10.41NASDD203 pKa = 4.53DD204 pKa = 4.2KK205 pKa = 11.8NGDD208 pKa = 3.75GVPDD212 pKa = 5.8PITYY216 pKa = 10.91ANDD219 pKa = 3.16AEE221 pKa = 5.06FYY223 pKa = 10.34QDD225 pKa = 3.33TYY227 pKa = 11.18QFSIMSYY234 pKa = 8.77FSHH237 pKa = 6.91RR238 pKa = 11.84FSGAFGSVNWATGGYY253 pKa = 8.43YY254 pKa = 7.37QTPQTPMVHH263 pKa = 7.27DD264 pKa = 4.17IAAAQEE270 pKa = 4.27LYY272 pKa = 10.84GVEE275 pKa = 4.06TTTRR279 pKa = 11.84TGNTVYY285 pKa = 10.81GFGSTADD292 pKa = 3.34RR293 pKa = 11.84AVFDD297 pKa = 4.3FSINKK302 pKa = 9.33NPFLTIYY309 pKa = 10.52DD310 pKa = 3.62AGGTDD315 pKa = 3.8MLDD318 pKa = 3.1LSGFTGGRR326 pKa = 11.84ITLDD330 pKa = 3.31LRR332 pKa = 11.84PGAFSTGYY340 pKa = 10.16NYY342 pKa = 10.67GVEE345 pKa = 4.36AEE347 pKa = 4.57LDD349 pKa = 3.89TVGLGNLTQAQWNALYY365 pKa = 10.87DD366 pKa = 3.84GVLGSNPGFLSDD378 pKa = 5.22NIGIAYY384 pKa = 7.28NTTVEE389 pKa = 3.97NGRR392 pKa = 11.84TGAGNDD398 pKa = 3.41VLRR401 pKa = 11.84GNDD404 pKa = 3.28VANSLFGGAGADD416 pKa = 3.4NYY418 pKa = 10.06TGAGGADD425 pKa = 2.87RR426 pKa = 11.84FVIEE430 pKa = 4.82QIGFTDD436 pKa = 4.71TITDD440 pKa = 4.29FAAGVDD446 pKa = 3.97KK447 pKa = 10.9IDD449 pKa = 4.51LKK451 pKa = 11.38AIDD454 pKa = 4.3AVTGGGDD461 pKa = 3.21NAFGFIGSAAFTAAGQLRR479 pKa = 11.84TYY481 pKa = 7.22TTSGVNYY488 pKa = 10.01VAGDD492 pKa = 3.37VDD494 pKa = 4.34GNGVADD500 pKa = 4.08FVINLGSANVAAGDD514 pKa = 3.77FFLL517 pKa = 6.24

Molecular weight: 54.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G7YT78|A0A6G7YT78_9SPHN Amidohydrolase OS=Sphingomonas sp. HDW15B OX=2714943 GN=G7077_04425 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.6GFRR19 pKa = 11.84HH20 pKa = 5.83RR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.07VLNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2594

0

2594

771404

33

1789

297.4

32.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.546 ± 0.072

0.816 ± 0.016

5.838 ± 0.041

5.709 ± 0.037

3.57 ± 0.027

8.805 ± 0.045

1.956 ± 0.022

4.84 ± 0.031

3.284 ± 0.033

10.005 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.26 ± 0.025

2.68 ± 0.035

5.29 ± 0.034

3.331 ± 0.027

7.434 ± 0.041

5.647 ± 0.034

5.126 ± 0.034

7.268 ± 0.037

1.421 ± 0.022

2.173 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski