Amino acid dipepetide frequency for Sphingomonas sp. HDW15B

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.19AlaAla: 18.19 ± 0.22
1.032AlaCys: 1.032 ± 0.039
7.34AlaAsp: 7.34 ± 0.105
7.788AlaGlu: 7.788 ± 0.132
4.059AlaPhe: 4.059 ± 0.081
10.723AlaGly: 10.723 ± 0.136
2.095AlaHis: 2.095 ± 0.062
6.224AlaIle: 6.224 ± 0.102
4.3AlaLys: 4.3 ± 0.095
12.805AlaLeu: 12.805 ± 0.165
3.424AlaMet: 3.424 ± 0.081
3.131AlaAsn: 3.131 ± 0.068
5.979AlaPro: 5.979 ± 0.1
4.256AlaGln: 4.256 ± 0.08
8.89AlaArg: 8.89 ± 0.141
6.624AlaSer: 6.624 ± 0.096
6.108AlaThr: 6.108 ± 0.089
8.613AlaVal: 8.613 ± 0.103
1.483AlaTrp: 1.483 ± 0.047
2.4AlaTyr: 2.4 ± 0.052
0.0AlaXaa: 0.0 ± 0.0
Cys
0.967CysAla: 0.967 ± 0.034
0.083CysCys: 0.083 ± 0.01
0.525CysAsp: 0.525 ± 0.027
0.402CysGlu: 0.402 ± 0.021
0.324CysPhe: 0.324 ± 0.019
0.923CysGly: 0.923 ± 0.036
0.197CysHis: 0.197 ± 0.015
0.394CysIle: 0.394 ± 0.023
0.194CysLys: 0.194 ± 0.016
0.752CysLeu: 0.752 ± 0.036
0.152CysMet: 0.152 ± 0.013
0.222CysAsn: 0.222 ± 0.015
0.447CysPro: 0.447 ± 0.026
0.194CysGln: 0.194 ± 0.016
0.634CysArg: 0.634 ± 0.027
0.487CysSer: 0.487 ± 0.027
0.421CysThr: 0.421 ± 0.024
0.539CysVal: 0.539 ± 0.029
0.13CysTrp: 0.13 ± 0.014
0.172CysTyr: 0.172 ± 0.017
0.0CysXaa: 0.0 ± 0.0
Asp
6.859AspAla: 6.859 ± 0.092
0.538AspCys: 0.538 ± 0.027
3.186AspAsp: 3.186 ± 0.072
3.805AspGlu: 3.805 ± 0.077
2.131AspPhe: 2.131 ± 0.05
5.312AspGly: 5.312 ± 0.091
1.282AspHis: 1.282 ± 0.047
2.63AspIle: 2.63 ± 0.058
1.776AspLys: 1.776 ± 0.056
6.037AspLeu: 6.037 ± 0.09
1.132AspMet: 1.132 ± 0.035
1.412AspAsn: 1.412 ± 0.047
3.643AspPro: 3.643 ± 0.071
2.022AspGln: 2.022 ± 0.056
4.791AspArg: 4.791 ± 0.086
2.481AspSer: 2.481 ± 0.062
2.379AspThr: 2.379 ± 0.062
4.303AspVal: 4.303 ± 0.079
1.094AspTrp: 1.094 ± 0.042
1.571AspTyr: 1.571 ± 0.045
0.0AspXaa: 0.0 ± 0.0
Glu
7.607GluAla: 7.607 ± 0.122
0.375GluCys: 0.375 ± 0.024
2.792GluAsp: 2.792 ± 0.064
3.402GluGlu: 3.402 ± 0.069
1.661GluPhe: 1.661 ± 0.048
4.581GluGly: 4.581 ± 0.082
1.26GluHis: 1.26 ± 0.039
2.781GluIle: 2.781 ± 0.06
2.027GluLys: 2.027 ± 0.062
5.766GluLeu: 5.766 ± 0.092
1.378GluMet: 1.378 ± 0.038
1.372GluAsn: 1.372 ± 0.045
2.936GluPro: 2.936 ± 0.063
2.525GluGln: 2.525 ± 0.064
5.38GluArg: 5.38 ± 0.102
2.402GluSer: 2.402 ± 0.057
2.9GluThr: 2.9 ± 0.062
4.15GluVal: 4.15 ± 0.08
0.812GluTrp: 0.812 ± 0.033
0.985GluTyr: 0.985 ± 0.035
0.0GluXaa: 0.0 ± 0.0
Phe
4.522PheAla: 4.522 ± 0.08
0.32PheCys: 0.32 ± 0.018
2.766PheAsp: 2.766 ± 0.07
2.144PheGlu: 2.144 ± 0.051
1.263PhePhe: 1.263 ± 0.041
3.6PheGly: 3.6 ± 0.072
0.751PheHis: 0.751 ± 0.032
1.466PheIle: 1.466 ± 0.044
0.949PheLys: 0.949 ± 0.035
3.164PheLeu: 3.164 ± 0.075
0.703PheMet: 0.703 ± 0.03
1.079PheAsn: 1.079 ± 0.037
1.548PhePro: 1.548 ± 0.046
0.966PheGln: 0.966 ± 0.036
2.335PheArg: 2.335 ± 0.051
2.091PheSer: 2.091 ± 0.057
2.013PheThr: 2.013 ± 0.053
2.656PheVal: 2.656 ± 0.057
0.48PheTrp: 0.48 ± 0.026
0.882PheTyr: 0.882 ± 0.033
0.0PheXaa: 0.0 ± 0.0
Gly
9.476GlyAla: 9.476 ± 0.118
0.94GlyCys: 0.94 ± 0.032
4.894GlyAsp: 4.894 ± 0.093
4.979GlyGlu: 4.979 ± 0.087
3.64GlyPhe: 3.64 ± 0.069
8.164GlyGly: 8.164 ± 0.144
1.773GlyHis: 1.773 ± 0.052
4.348GlyIle: 4.348 ± 0.089
3.639GlyLys: 3.639 ± 0.077
8.284GlyLeu: 8.284 ± 0.105
2.097GlyMet: 2.097 ± 0.053
2.394GlyAsn: 2.394 ± 0.065
3.717GlyPro: 3.717 ± 0.07
3.09GlyGln: 3.09 ± 0.067
6.557GlyArg: 6.557 ± 0.112
5.319GlySer: 5.319 ± 0.089
4.844GlyThr: 4.844 ± 0.092
6.143GlyVal: 6.143 ± 0.098
1.553GlyTrp: 1.553 ± 0.049
2.193GlyTyr: 2.193 ± 0.062
0.0GlyXaa: 0.0 ± 0.0
His
2.187HisAla: 2.187 ± 0.059
0.213HisCys: 0.213 ± 0.016
1.147HisAsp: 1.147 ± 0.039
1.018HisGlu: 1.018 ± 0.038
0.795HisPhe: 0.795 ± 0.036
1.872HisGly: 1.872 ± 0.051
0.556HisHis: 0.556 ± 0.027
0.852HisIle: 0.852 ± 0.035
0.526HisLys: 0.526 ± 0.029
2.016HisLeu: 2.016 ± 0.062
0.436HisMet: 0.436 ± 0.024
0.456HisAsn: 0.456 ± 0.025
1.298HisPro: 1.298 ± 0.041
0.607HisGln: 0.607 ± 0.032
1.563HisArg: 1.563 ± 0.047
1.02HisSer: 1.02 ± 0.036
0.662HisThr: 0.662 ± 0.029
1.451HisVal: 1.451 ± 0.04
0.36HisTrp: 0.36 ± 0.023
0.528HisTyr: 0.528 ± 0.03
0.0HisXaa: 0.0 ± 0.0
Ile
7.169IleAla: 7.169 ± 0.119
0.461IleCys: 0.461 ± 0.025
3.521IleAsp: 3.521 ± 0.066
3.268IleGlu: 3.268 ± 0.063
1.579IlePhe: 1.579 ± 0.045
4.992IleGly: 4.992 ± 0.086
0.874IleHis: 0.874 ± 0.033
1.996IleIle: 1.996 ± 0.049
1.234IleLys: 1.234 ± 0.051
4.063IleLeu: 4.063 ± 0.082
0.857IleMet: 0.857 ± 0.034
1.312IleAsn: 1.312 ± 0.041
2.17IlePro: 2.17 ± 0.048
1.215IleGln: 1.215 ± 0.041
3.269IleArg: 3.269 ± 0.06
2.599IleSer: 2.599 ± 0.058
2.375IleThr: 2.375 ± 0.055
3.897IleVal: 3.897 ± 0.07
0.524IleTrp: 0.524 ± 0.028
1.027IleTyr: 1.027 ± 0.041
0.0IleXaa: 0.0 ± 0.0
Lys
4.111LysAla: 4.111 ± 0.088
0.167LysCys: 0.167 ± 0.015
1.745LysAsp: 1.745 ± 0.056
1.524LysGlu: 1.524 ± 0.045
0.911LysPhe: 0.911 ± 0.035
2.702LysGly: 2.702 ± 0.071
0.598LysHis: 0.598 ± 0.028
1.571LysIle: 1.571 ± 0.046
1.239LysLys: 1.239 ± 0.049
3.671LysLeu: 3.671 ± 0.075
0.775LysMet: 0.775 ± 0.036
0.848LysAsn: 0.848 ± 0.033
2.081LysPro: 2.081 ± 0.055
1.077LysGln: 1.077 ± 0.039
2.498LysArg: 2.498 ± 0.061
1.887LysSer: 1.887 ± 0.056
1.729LysThr: 1.729 ± 0.048
2.633LysVal: 2.633 ± 0.06
0.426LysTrp: 0.426 ± 0.023
0.648LysTyr: 0.648 ± 0.033
0.0LysXaa: 0.0 ± 0.0
Leu
12.928LeuAla: 12.928 ± 0.158
0.725LeuCys: 0.725 ± 0.031
5.929LeuAsp: 5.929 ± 0.086
5.314LeuGlu: 5.314 ± 0.102
3.724LeuPhe: 3.724 ± 0.068
8.328LeuGly: 8.328 ± 0.107
1.803LeuHis: 1.803 ± 0.053
5.136LeuIle: 5.136 ± 0.09
3.613LeuLys: 3.613 ± 0.076
10.235LeuLeu: 10.235 ± 0.157
1.978LeuMet: 1.978 ± 0.053
2.717LeuAsn: 2.717 ± 0.059
5.461LeuPro: 5.461 ± 0.099
3.102LeuGln: 3.102 ± 0.067
6.846LeuArg: 6.846 ± 0.101
6.321LeuSer: 6.321 ± 0.098
5.678LeuThr: 5.678 ± 0.097
7.047LeuVal: 7.047 ± 0.103
1.167LeuTrp: 1.167 ± 0.047
1.999LeuTyr: 1.999 ± 0.054
0.0LeuXaa: 0.0 ± 0.0
Met
2.858MetAla: 2.858 ± 0.059
0.149MetCys: 0.149 ± 0.014
1.088MetAsp: 1.088 ± 0.04
0.948MetGlu: 0.948 ± 0.038
0.707MetPhe: 0.707 ± 0.03
1.641MetGly: 1.641 ± 0.044
0.426MetHis: 0.426 ± 0.025
1.237MetIle: 1.237 ± 0.041
0.924MetLys: 0.924 ± 0.036
2.423MetLeu: 2.423 ± 0.05
0.607MetMet: 0.607 ± 0.029
0.722MetAsn: 0.722 ± 0.032
1.357MetPro: 1.357 ± 0.043
0.697MetGln: 0.697 ± 0.031
1.72MetArg: 1.72 ± 0.047
1.451MetSer: 1.451 ± 0.043
1.544MetThr: 1.544 ± 0.043
1.579MetVal: 1.579 ± 0.048
0.253MetTrp: 0.253 ± 0.019
0.268MetTyr: 0.268 ± 0.019
0.0MetXaa: 0.0 ± 0.0
Asn
3.17AsnAla: 3.17 ± 0.068
0.24AsnCys: 0.24 ± 0.02
1.552AsnAsp: 1.552 ± 0.043
1.289AsnGlu: 1.289 ± 0.035
0.942AsnPhe: 0.942 ± 0.042
2.602AsnGly: 2.602 ± 0.077
0.519AsnHis: 0.519 ± 0.028
1.361AsnIle: 1.361 ± 0.049
0.7AsnLys: 0.7 ± 0.034
2.678AsnLeu: 2.678 ± 0.068
0.578AsnMet: 0.578 ± 0.03
0.756AsnAsn: 0.756 ± 0.035
1.811AsnPro: 1.811 ± 0.047
0.862AsnGln: 0.862 ± 0.033
1.893AsnArg: 1.893 ± 0.056
1.479AsnSer: 1.479 ± 0.042
1.102AsnThr: 1.102 ± 0.046
2.048AsnVal: 2.048 ± 0.055
0.489AsnTrp: 0.489 ± 0.029
0.729AsnTyr: 0.729 ± 0.039
0.0AsnXaa: 0.0 ± 0.0
Pro
6.537ProAla: 6.537 ± 0.095
0.342ProCys: 0.342 ± 0.02
3.6ProAsp: 3.6 ± 0.07
3.573ProGlu: 3.573 ± 0.071
1.97ProPhe: 1.97 ± 0.047
4.523ProGly: 4.523 ± 0.073
1.001ProHis: 1.001 ± 0.038
2.388ProIle: 2.388 ± 0.054
1.764ProLys: 1.764 ± 0.051
4.949ProLeu: 4.949 ± 0.093
1.181ProMet: 1.181 ± 0.04
1.479ProAsn: 1.479 ± 0.046
2.72ProPro: 2.72 ± 0.081
1.916ProGln: 1.916 ± 0.054
2.976ProArg: 2.976 ± 0.057
3.107ProSer: 3.107 ± 0.061
2.709ProThr: 2.709 ± 0.059
4.351ProVal: 4.351 ± 0.083
0.69ProTrp: 0.69 ± 0.035
1.127ProTyr: 1.127 ± 0.043
0.0ProXaa: 0.0 ± 0.0
Gln
4.358GlnAla: 4.358 ± 0.071
0.219GlnCys: 0.219 ± 0.017
1.394GlnAsp: 1.394 ± 0.042
1.523GlnGlu: 1.523 ± 0.043
1.099GlnPhe: 1.099 ± 0.039
2.543GlnGly: 2.543 ± 0.061
0.704GlnHis: 0.704 ± 0.032
1.629GlnIle: 1.629 ± 0.047
0.97GlnLys: 0.97 ± 0.037
3.682GlnLeu: 3.682 ± 0.079
0.888GlnMet: 0.888 ± 0.034
0.808GlnAsn: 0.808 ± 0.035
2.117GlnPro: 2.117 ± 0.053
1.399GlnGln: 1.399 ± 0.044
2.82GlnArg: 2.82 ± 0.058
2.03GlnSer: 2.03 ± 0.053
1.53GlnThr: 1.53 ± 0.046
2.514GlnVal: 2.514 ± 0.065
0.461GlnTrp: 0.461 ± 0.025
0.62GlnTyr: 0.62 ± 0.028
0.0GlnXaa: 0.0 ± 0.0
Arg
8.171ArgAla: 8.171 ± 0.118
0.592ArgCys: 0.592 ± 0.028
4.077ArgAsp: 4.077 ± 0.077
4.187ArgGlu: 4.187 ± 0.074
3.181ArgPhe: 3.181 ± 0.054
5.314ArgGly: 5.314 ± 0.073
1.605ArgHis: 1.605 ± 0.049
3.943ArgIle: 3.943 ± 0.065
2.405ArgLys: 2.405 ± 0.058
8.387ArgLeu: 8.387 ± 0.127
1.908ArgMet: 1.908 ± 0.049
2.004ArgAsn: 2.004 ± 0.046
3.705ArgPro: 3.705 ± 0.078
2.632ArgGln: 2.632 ± 0.062
6.143ArgArg: 6.143 ± 0.102
4.358ArgSer: 4.358 ± 0.078
3.8ArgThr: 3.8 ± 0.072
4.843ArgVal: 4.843 ± 0.085
1.287ArgTrp: 1.287 ± 0.037
1.801ArgTyr: 1.801 ± 0.042
0.0ArgXaa: 0.0 ± 0.0
Ser
6.86SerAla: 6.86 ± 0.101
0.426SerCys: 0.426 ± 0.024
3.308SerAsp: 3.308 ± 0.062
2.954SerGlu: 2.954 ± 0.06
2.328SerPhe: 2.328 ± 0.046
5.796SerGly: 5.796 ± 0.102
1.018SerHis: 1.018 ± 0.043
2.637SerIle: 2.637 ± 0.06
1.812SerLys: 1.812 ± 0.059
5.555SerLeu: 5.555 ± 0.086
1.212SerMet: 1.212 ± 0.036
1.602SerAsn: 1.602 ± 0.045
2.965SerPro: 2.965 ± 0.06
1.605SerGln: 1.605 ± 0.052
3.928SerArg: 3.928 ± 0.068
3.434SerSer: 3.434 ± 0.087
2.786SerThr: 2.786 ± 0.06
3.998SerVal: 3.998 ± 0.066
0.878SerTrp: 0.878 ± 0.037
1.369SerTyr: 1.369 ± 0.042
0.0SerXaa: 0.0 ± 0.0
Thr
6.134ThrAla: 6.134 ± 0.102
0.415ThrCys: 0.415 ± 0.028
2.83ThrAsp: 2.83 ± 0.067
2.499ThrGlu: 2.499 ± 0.064
1.921ThrPhe: 1.921 ± 0.061
5.084ThrGly: 5.084 ± 0.089
0.88ThrHis: 0.88 ± 0.033
2.781ThrIle: 2.781 ± 0.054
1.453ThrLys: 1.453 ± 0.047
5.329ThrLeu: 5.329 ± 0.084
1.092ThrMet: 1.092 ± 0.039
1.364ThrAsn: 1.364 ± 0.047
3.224ThrPro: 3.224 ± 0.076
1.452ThrGln: 1.452 ± 0.046
3.334ThrArg: 3.334 ± 0.062
2.969ThrSer: 2.969 ± 0.064
2.643ThrThr: 2.643 ± 0.061
4.063ThrVal: 4.063 ± 0.079
0.598ThrTrp: 0.598 ± 0.03
1.194ThrTyr: 1.194 ± 0.042
0.0ThrXaa: 0.0 ± 0.0
Val
9.58ValAla: 9.58 ± 0.13
0.554ValCys: 0.554 ± 0.028
4.541ValAsp: 4.541 ± 0.084
4.686ValGlu: 4.686 ± 0.072
2.055ValPhe: 2.055 ± 0.051
6.264ValGly: 6.264 ± 0.103
1.414ValHis: 1.414 ± 0.043
3.587ValIle: 3.587 ± 0.07
2.234ValLys: 2.234 ± 0.065
6.442ValLeu: 6.442 ± 0.087
1.449ValMet: 1.449 ± 0.045
2.031ValAsn: 2.031 ± 0.049
3.968ValPro: 3.968 ± 0.069
2.301ValGln: 2.301 ± 0.057
5.496ValArg: 5.496 ± 0.088
4.177ValSer: 4.177 ± 0.08
4.296ValThr: 4.296 ± 0.095
5.395ValVal: 5.395 ± 0.098
0.889ValTrp: 0.889 ± 0.031
1.322ValTyr: 1.322 ± 0.048
0.0ValXaa: 0.0 ± 0.0
Trp
1.374TrpAla: 1.374 ± 0.043
0.131TrpCys: 0.131 ± 0.012
0.697TrpAsp: 0.697 ± 0.034
0.602TrpGlu: 0.602 ± 0.026
0.504TrpPhe: 0.504 ± 0.028
0.923TrpGly: 0.923 ± 0.037
0.359TrpHis: 0.359 ± 0.024
0.674TrpIle: 0.674 ± 0.029
0.486TrpLys: 0.486 ± 0.025
1.694TrpLeu: 1.694 ± 0.048
0.368TrpMet: 0.368 ± 0.026
0.477TrpAsn: 0.477 ± 0.026
0.699TrpPro: 0.699 ± 0.028
0.62TrpGln: 0.62 ± 0.031
1.292TrpArg: 1.292 ± 0.041
0.996TrpSer: 0.996 ± 0.044
0.806TrpThr: 0.806 ± 0.037
0.914TrpVal: 0.914 ± 0.036
0.275TrpTrp: 0.275 ± 0.02
0.319TrpTyr: 0.319 ± 0.021
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.405TyrAla: 2.405 ± 0.058
0.239TyrCys: 0.239 ± 0.021
1.452TyrAsp: 1.452 ± 0.047
1.187TyrGlu: 1.187 ± 0.039
0.901TyrPhe: 0.901 ± 0.036
2.158TyrGly: 2.158 ± 0.059
0.447TyrHis: 0.447 ± 0.024
0.766TyrIle: 0.766 ± 0.033
0.596TyrLys: 0.596 ± 0.032
2.12TyrLeu: 2.12 ± 0.053
0.384TyrMet: 0.384 ± 0.027
0.613TyrAsn: 0.613 ± 0.032
1.059TyrPro: 1.059 ± 0.038
0.769TyrGln: 0.769 ± 0.032
1.977TyrArg: 1.977 ± 0.055
1.238TyrSer: 1.238 ± 0.043
0.932TyrThr: 0.932 ± 0.041
1.547TyrVal: 1.547 ± 0.046
0.363TyrTrp: 0.363 ± 0.023
0.576TyrTyr: 0.576 ± 0.032
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2594 proteins (771404 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski