Solirubrum puertoriconensis
Average proteome isoelectric point is 6.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4037 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0W8EE65|A0A0W8EE65_9BACT Metallophos domain-containing protein OS=Solirubrum puertoriconensis OX=1751427 GN=ASU33_06725 PE=4 SV=1
MM1 pKa = 7.21 NRR3 pKa = 11.84 TRR5 pKa = 11.84 IWCMSGALCALLALGSCGNDD25 pKa = 3.07 GNSNAGSGMGADD37 pKa = 4.14 GTGSEE42 pKa = 4.7 PGSATSTEE50 pKa = 4.26 TTTDD54 pKa = 3.09 MGSTDD59 pKa = 3.2 TMGTGTGAGTTGSTSSGTTGGGVTTGGGMSGTTSSAGSTAGTTAGSTGGGATTNEE114 pKa = 4.1 ATTGGTTGNSGAGGSTTSGSTTNN137 pKa = 3.5
Molecular weight: 12.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.792
IPC2_protein 3.923
IPC_protein 3.706
Toseland 3.541
ProMoST 3.923
Dawson 3.719
Bjellqvist 3.897
Wikipedia 3.706
Rodwell 3.567
Grimsley 3.465
Solomon 3.668
Lehninger 3.63
Nozaki 3.884
DTASelect 4.037
Thurlkill 3.643
EMBOSS 3.706
Sillero 3.846
Patrickios 1.888
IPC_peptide 3.668
IPC2_peptide 3.808
IPC2.peptide.svr19 3.776
Protein with the highest isoelectric point:
>tr|A0A0W8EIK8|A0A0W8EIK8_9BACT AsmA_2 domain-containing protein OS=Solirubrum puertoriconensis OX=1751427 GN=ASU33_10455 PE=4 SV=1
MM1 pKa = 7.13 PHH3 pKa = 5.91 AVKK6 pKa = 10.45 YY7 pKa = 10.92 ASVFLLSMVKK17 pKa = 10.42 FFGGPLAGAAAGLSFVPTWVLTVAGMMTSVLVFSGLGRR55 pKa = 11.84 IFVAHH60 pKa = 5.77 LQRR63 pKa = 11.84 RR64 pKa = 11.84 RR65 pKa = 11.84 RR66 pKa = 11.84 LRR68 pKa = 11.84 HH69 pKa = 5.81 KK70 pKa = 10.48 PVFTPRR76 pKa = 11.84 ARR78 pKa = 11.84 QIVRR82 pKa = 11.84 VFKK85 pKa = 10.71 RR86 pKa = 11.84 FGIGGIAFLTPVLFTPIGGTVIATLLGVKK115 pKa = 9.49 RR116 pKa = 11.84 RR117 pKa = 11.84 HH118 pKa = 6.33 ILLHH122 pKa = 5.5 MLWSAMFWGAAFTFITIKK140 pKa = 10.51 FSHH143 pKa = 6.66 LPIFHH148 pKa = 7.13 RR149 pKa = 4.54
Molecular weight: 16.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.464
IPC2_protein 11.184
IPC_protein 12.588
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.749
Wikipedia 13.217
Rodwell 12.427
Grimsley 12.793
Solomon 13.247
Lehninger 13.144
Nozaki 12.749
DTASelect 12.749
Thurlkill 12.749
EMBOSS 13.247
Sillero 12.749
Patrickios 12.149
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.093
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4037
0
4037
1338913
38
2581
331.7
36.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.328 ± 0.051
0.734 ± 0.013
4.799 ± 0.03
5.586 ± 0.046
3.965 ± 0.027
7.618 ± 0.045
2.089 ± 0.022
4.284 ± 0.029
3.882 ± 0.036
10.818 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.948 ± 0.018
3.89 ± 0.042
5.141 ± 0.031
4.96 ± 0.032
6.382 ± 0.042
5.443 ± 0.032
5.996 ± 0.048
7.312 ± 0.032
1.282 ± 0.016
3.542 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here