Bacillus phage Pavlov

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Pagevirus; unclassified Pagevirus

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0H4TGA9|A0A0H4TGA9_9CAUD Carboxypeptidase A OS=Bacillus phage Pavlov OX=1675598 GN=CPT_Pavlov14 PE=4 SV=1
MM1 pKa = 7.25ATFIEE6 pKa = 4.67TMPKK10 pKa = 9.96HH11 pKa = 6.61DD12 pKa = 5.3DD13 pKa = 3.47EE14 pKa = 4.58MLGEE18 pKa = 4.46VFNMSPIGEE27 pKa = 4.37ANGEE31 pKa = 4.2FIDD34 pKa = 4.97GYY36 pKa = 10.27MRR38 pKa = 11.84TAFFTAHH45 pKa = 6.18YY46 pKa = 9.79EE47 pKa = 4.36SIANATASPLTQTNYY62 pKa = 10.12EE63 pKa = 3.99ALKK66 pKa = 10.71AINKK70 pKa = 7.01YY71 pKa = 9.14WKK73 pKa = 9.91INAINWRR80 pKa = 11.84KK81 pKa = 9.88NGVFPVYY88 pKa = 10.46NIPADD93 pKa = 3.65KK94 pKa = 11.02LIDD97 pKa = 3.72EE98 pKa = 4.87EE99 pKa = 4.58EE100 pKa = 4.27AFLLEE105 pKa = 4.78MYY107 pKa = 8.75PPPAEE112 pKa = 4.15EE113 pKa = 4.01EE114 pKa = 4.2QPTTEE119 pKa = 4.04EE120 pKa = 4.1NTTEE124 pKa = 4.29GAA126 pKa = 3.48

Molecular weight: 14.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0H4TGB9|A0A0H4TGB9_9CAUD Endolysin OS=Bacillus phage Pavlov OX=1675598 GN=CPT_Pavlov24 PE=4 SV=1
MM1 pKa = 7.31FGGGRR6 pKa = 11.84SEE8 pKa = 4.12KK9 pKa = 10.59DD10 pKa = 2.7IAAGRR15 pKa = 11.84FDD17 pKa = 3.52CSSYY21 pKa = 10.84VRR23 pKa = 11.84WAYY26 pKa = 10.5SQLGINLGPLTSTSTEE42 pKa = 3.87TLKK45 pKa = 11.4NKK47 pKa = 7.98GTAVKK52 pKa = 10.53GGIKK56 pKa = 9.95NAQPGDD62 pKa = 3.72LLFFDD67 pKa = 4.51TYY69 pKa = 10.87KK70 pKa = 11.08KK71 pKa = 10.13NGHH74 pKa = 4.46VVIYY78 pKa = 10.36LGNNQFIGAQGKK90 pKa = 7.34TGVGIVDD97 pKa = 3.64MNSSYY102 pKa = 11.42GKK104 pKa = 10.16YY105 pKa = 7.28FTKK108 pKa = 10.41RR109 pKa = 11.84FSGNIRR115 pKa = 11.84RR116 pKa = 11.84IAGGGNVGAALGGGGAVMSGAGTGTAYY143 pKa = 10.32RR144 pKa = 11.84QAPSNLMGPINNAAKK159 pKa = 10.29QYY161 pKa = 10.27GVNPNLIAAIIKK173 pKa = 10.22KK174 pKa = 10.2EE175 pKa = 4.13STFKK179 pKa = 11.0SGLTSSAGAKK189 pKa = 10.33GYY191 pKa = 7.86MQLMPATARR200 pKa = 11.84AMGVKK205 pKa = 10.22NPWDD209 pKa = 3.58TQQNINGGTKK219 pKa = 10.51YY220 pKa = 9.98IAQQLKK226 pKa = 8.97TYY228 pKa = 10.29KK229 pKa = 10.78NNIPLALAAYY239 pKa = 9.31NWGPGNLNKK248 pKa = 10.5AIRR251 pKa = 11.84KK252 pKa = 9.59AGGSKK257 pKa = 10.02DD258 pKa = 2.92WNQIRR263 pKa = 11.84RR264 pKa = 11.84FAPKK268 pKa = 8.44EE269 pKa = 3.76TRR271 pKa = 11.84DD272 pKa = 3.79YY273 pKa = 11.28VDD275 pKa = 5.67KK276 pKa = 11.37IMGWRR281 pKa = 3.39

Molecular weight: 29.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

12796

30

1679

255.9

28.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.901 ± 0.361

0.696 ± 0.148

5.65 ± 0.259

7.018 ± 0.527

4.126 ± 0.199

7.002 ± 0.371

1.594 ± 0.172

6.08 ± 0.372

8.276 ± 0.467

7.299 ± 0.356

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.774 ± 0.195

6.252 ± 0.238

3.634 ± 0.311

4.736 ± 0.628

4.173 ± 0.255

5.689 ± 0.267

5.978 ± 0.481

5.978 ± 0.238

1.196 ± 0.126

3.947 ± 0.335
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski