Amino acid dipepetide frequency for Bacillus phage Pavlov

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.05AlaAla: 8.05 ± 0.861
0.625AlaCys: 0.625 ± 0.213
5.158AlaAsp: 5.158 ± 0.399
5.471AlaGlu: 5.471 ± 0.752
3.126AlaPhe: 3.126 ± 0.406
6.956AlaGly: 6.956 ± 0.874
0.86AlaHis: 0.86 ± 0.316
3.908AlaIle: 3.908 ± 0.555
5.393AlaLys: 5.393 ± 0.718
5.862AlaLeu: 5.862 ± 0.682
2.267AlaMet: 2.267 ± 0.367
3.439AlaAsn: 3.439 ± 0.574
3.204AlaPro: 3.204 ± 0.623
3.048AlaGln: 3.048 ± 0.789
4.533AlaArg: 4.533 ± 0.668
3.361AlaSer: 3.361 ± 0.506
4.924AlaThr: 4.924 ± 0.667
4.924AlaVal: 4.924 ± 0.639
1.016AlaTrp: 1.016 ± 0.27
2.892AlaTyr: 2.892 ± 0.517
0.0AlaXaa: 0.0 ± 0.0
Cys
0.469CysAla: 0.469 ± 0.173
0.156CysCys: 0.156 ± 0.103
0.625CysAsp: 0.625 ± 0.242
0.703CysGlu: 0.703 ± 0.269
0.078CysPhe: 0.078 ± 0.084
0.469CysGly: 0.469 ± 0.201
0.156CysHis: 0.156 ± 0.108
0.234CysIle: 0.234 ± 0.142
0.547CysLys: 0.547 ± 0.191
0.313CysLeu: 0.313 ± 0.141
0.313CysMet: 0.313 ± 0.147
0.469CysAsn: 0.469 ± 0.246
0.391CysPro: 0.391 ± 0.202
0.234CysGln: 0.234 ± 0.143
0.313CysArg: 0.313 ± 0.148
0.391CysSer: 0.391 ± 0.174
0.547CysThr: 0.547 ± 0.208
0.234CysVal: 0.234 ± 0.149
0.156CysTrp: 0.156 ± 0.112
0.156CysTyr: 0.156 ± 0.112
0.0CysXaa: 0.0 ± 0.0
Asp
3.517AspAla: 3.517 ± 0.409
0.625AspCys: 0.625 ± 0.301
3.595AspAsp: 3.595 ± 0.599
3.751AspGlu: 3.751 ± 0.525
3.283AspPhe: 3.283 ± 0.416
4.22AspGly: 4.22 ± 0.774
0.547AspHis: 0.547 ± 0.152
4.689AspIle: 4.689 ± 0.619
3.83AspLys: 3.83 ± 0.486
4.455AspLeu: 4.455 ± 0.611
1.641AspMet: 1.641 ± 0.492
3.283AspAsn: 3.283 ± 0.653
1.485AspPro: 1.485 ± 0.361
1.876AspGln: 1.876 ± 0.469
1.876AspArg: 1.876 ± 0.4
2.892AspSer: 2.892 ± 0.334
2.892AspThr: 2.892 ± 0.622
4.22AspVal: 4.22 ± 0.598
0.86AspTrp: 0.86 ± 0.304
2.97AspTyr: 2.97 ± 0.362
0.0AspXaa: 0.0 ± 0.0
Glu
5.315GluAla: 5.315 ± 0.824
0.703GluCys: 0.703 ± 0.232
2.579GluAsp: 2.579 ± 0.404
4.689GluGlu: 4.689 ± 0.936
2.501GluPhe: 2.501 ± 0.41
4.142GluGly: 4.142 ± 0.576
1.719GluHis: 1.719 ± 0.39
5.158GluIle: 5.158 ± 0.724
6.721GluLys: 6.721 ± 0.907
5.705GluLeu: 5.705 ± 1.041
2.892GluMet: 2.892 ± 0.637
4.377GluAsn: 4.377 ± 0.671
2.11GluPro: 2.11 ± 0.293
3.986GluGln: 3.986 ± 0.745
2.97GluArg: 2.97 ± 0.503
2.97GluSer: 2.97 ± 0.51
3.595GluThr: 3.595 ± 0.724
4.377GluVal: 4.377 ± 0.593
0.938GluTrp: 0.938 ± 0.325
2.735GluTyr: 2.735 ± 0.512
0.0GluXaa: 0.0 ± 0.0
Phe
1.719PheAla: 1.719 ± 0.315
0.313PheCys: 0.313 ± 0.155
2.423PheAsp: 2.423 ± 0.499
2.657PheGlu: 2.657 ± 0.576
1.798PhePhe: 1.798 ± 0.365
2.735PheGly: 2.735 ± 0.411
0.782PheHis: 0.782 ± 0.269
3.517PheIle: 3.517 ± 0.543
3.439PheLys: 3.439 ± 0.578
3.83PheLeu: 3.83 ± 0.558
1.094PheMet: 1.094 ± 0.255
3.439PheAsn: 3.439 ± 0.473
1.954PhePro: 1.954 ± 0.361
1.641PheGln: 1.641 ± 0.47
1.719PheArg: 1.719 ± 0.438
2.657PheSer: 2.657 ± 0.581
2.97PheThr: 2.97 ± 0.483
1.25PheVal: 1.25 ± 0.291
0.313PheTrp: 0.313 ± 0.139
1.016PheTyr: 1.016 ± 0.31
0.0PheXaa: 0.0 ± 0.0
Gly
5.862GlyAla: 5.862 ± 0.646
0.313GlyCys: 0.313 ± 0.193
2.579GlyAsp: 2.579 ± 0.432
4.611GlyGlu: 4.611 ± 0.706
2.423GlyPhe: 2.423 ± 0.412
6.018GlyGly: 6.018 ± 1.045
0.86GlyHis: 0.86 ± 0.242
3.83GlyIle: 3.83 ± 0.591
5.862GlyLys: 5.862 ± 1.023
5.471GlyLeu: 5.471 ± 0.748
2.345GlyMet: 2.345 ± 0.452
4.455GlyAsn: 4.455 ± 0.619
1.172GlyPro: 1.172 ± 0.364
2.579GlyGln: 2.579 ± 0.571
2.97GlyArg: 2.97 ± 0.432
3.986GlySer: 3.986 ± 0.686
5.393GlyThr: 5.393 ± 0.861
6.018GlyVal: 6.018 ± 0.617
0.782GlyTrp: 0.782 ± 0.219
2.501GlyTyr: 2.501 ± 0.436
0.0GlyXaa: 0.0 ± 0.0
His
0.86HisAla: 0.86 ± 0.299
0.234HisCys: 0.234 ± 0.134
1.798HisAsp: 1.798 ± 0.386
0.938HisGlu: 0.938 ± 0.355
0.703HisPhe: 0.703 ± 0.17
0.703HisGly: 0.703 ± 0.266
0.156HisHis: 0.156 ± 0.12
1.172HisIle: 1.172 ± 0.296
1.094HisLys: 1.094 ± 0.207
1.016HisLeu: 1.016 ± 0.301
0.313HisMet: 0.313 ± 0.2
1.25HisAsn: 1.25 ± 0.299
0.782HisPro: 0.782 ± 0.234
0.625HisGln: 0.625 ± 0.19
0.703HisArg: 0.703 ± 0.263
0.625HisSer: 0.625 ± 0.192
0.86HisThr: 0.86 ± 0.252
0.625HisVal: 0.625 ± 0.235
0.391HisTrp: 0.391 ± 0.163
1.094HisTyr: 1.094 ± 0.309
0.0HisXaa: 0.0 ± 0.0
Ile
4.299IleAla: 4.299 ± 0.671
0.391IleCys: 0.391 ± 0.172
5.08IleAsp: 5.08 ± 0.81
4.455IleGlu: 4.455 ± 0.777
2.267IlePhe: 2.267 ± 0.533
3.751IleGly: 3.751 ± 0.53
1.485IleHis: 1.485 ± 0.353
3.908IleIle: 3.908 ± 0.583
5.784IleLys: 5.784 ± 0.707
3.204IleLeu: 3.204 ± 0.62
1.563IleMet: 1.563 ± 0.355
4.455IleAsn: 4.455 ± 0.549
2.188IlePro: 2.188 ± 0.32
2.892IleGln: 2.892 ± 0.465
2.11IleArg: 2.11 ± 0.527
2.814IleSer: 2.814 ± 0.555
4.142IleThr: 4.142 ± 0.772
3.126IleVal: 3.126 ± 0.453
0.391IleTrp: 0.391 ± 0.163
2.501IleTyr: 2.501 ± 0.522
0.0IleXaa: 0.0 ± 0.0
Lys
7.19LysAla: 7.19 ± 0.754
0.782LysCys: 0.782 ± 0.256
4.064LysAsp: 4.064 ± 0.56
7.425LysGlu: 7.425 ± 1.078
2.735LysPhe: 2.735 ± 0.454
5.784LysGly: 5.784 ± 0.825
1.016LysHis: 1.016 ± 0.325
4.767LysIle: 4.767 ± 0.686
7.034LysLys: 7.034 ± 0.915
5.549LysLeu: 5.549 ± 0.67
2.423LysMet: 2.423 ± 0.359
5.627LysAsn: 5.627 ± 0.617
2.735LysPro: 2.735 ± 0.515
4.455LysGln: 4.455 ± 0.955
4.142LysArg: 4.142 ± 0.601
4.846LysSer: 4.846 ± 0.738
4.22LysThr: 4.22 ± 0.576
4.142LysVal: 4.142 ± 0.511
0.547LysTrp: 0.547 ± 0.188
3.283LysTyr: 3.283 ± 0.588
0.0LysXaa: 0.0 ± 0.0
Leu
5.08LeuAla: 5.08 ± 0.585
0.313LeuCys: 0.313 ± 0.176
3.439LeuAsp: 3.439 ± 0.529
6.096LeuGlu: 6.096 ± 0.841
2.657LeuPhe: 2.657 ± 0.446
4.689LeuGly: 4.689 ± 0.742
1.172LeuHis: 1.172 ± 0.377
4.064LeuIle: 4.064 ± 0.73
6.331LeuLys: 6.331 ± 0.777
5.158LeuLeu: 5.158 ± 0.826
2.345LeuMet: 2.345 ± 0.329
6.331LeuAsn: 6.331 ± 0.609
1.641LeuPro: 1.641 ± 0.435
2.657LeuGln: 2.657 ± 0.439
2.735LeuArg: 2.735 ± 0.561
5.158LeuSer: 5.158 ± 0.672
5.784LeuThr: 5.784 ± 0.739
3.751LeuVal: 3.751 ± 0.534
1.25LeuTrp: 1.25 ± 0.33
2.345LeuTyr: 2.345 ± 0.519
0.0LeuXaa: 0.0 ± 0.0
Met
2.188MetAla: 2.188 ± 0.421
0.234MetCys: 0.234 ± 0.139
1.798MetAsp: 1.798 ± 0.344
1.094MetGlu: 1.094 ± 0.303
1.563MetPhe: 1.563 ± 0.331
1.407MetGly: 1.407 ± 0.494
0.234MetHis: 0.234 ± 0.137
1.25MetIle: 1.25 ± 0.368
2.657MetLys: 2.657 ± 0.483
1.563MetLeu: 1.563 ± 0.443
0.547MetMet: 0.547 ± 0.235
1.954MetAsn: 1.954 ± 0.347
1.719MetPro: 1.719 ± 0.336
1.641MetGln: 1.641 ± 0.401
1.329MetArg: 1.329 ± 0.394
2.188MetSer: 2.188 ± 0.377
1.798MetThr: 1.798 ± 0.395
1.563MetVal: 1.563 ± 0.345
0.078MetTrp: 0.078 ± 0.087
0.938MetTyr: 0.938 ± 0.273
0.0MetXaa: 0.0 ± 0.0
Asn
6.487AsnAla: 6.487 ± 0.627
0.234AsnCys: 0.234 ± 0.163
3.439AsnAsp: 3.439 ± 0.522
3.673AsnGlu: 3.673 ± 0.611
2.579AsnPhe: 2.579 ± 0.402
4.455AsnGly: 4.455 ± 0.474
1.172AsnHis: 1.172 ± 0.291
3.126AsnIle: 3.126 ± 0.559
4.924AsnLys: 4.924 ± 0.777
3.83AsnLeu: 3.83 ± 0.665
2.032AsnMet: 2.032 ± 0.381
2.892AsnAsn: 2.892 ± 0.566
2.814AsnPro: 2.814 ± 0.782
2.892AsnGln: 2.892 ± 0.599
3.126AsnArg: 3.126 ± 0.543
3.048AsnSer: 3.048 ± 0.526
3.283AsnThr: 3.283 ± 0.476
5.002AsnVal: 5.002 ± 0.979
0.938AsnTrp: 0.938 ± 0.331
2.579AsnTyr: 2.579 ± 0.6
0.0AsnXaa: 0.0 ± 0.0
Pro
3.361ProAla: 3.361 ± 0.57
0.313ProCys: 0.313 ± 0.163
2.188ProAsp: 2.188 ± 0.48
2.97ProGlu: 2.97 ± 0.517
1.641ProPhe: 1.641 ± 0.37
2.501ProGly: 2.501 ± 0.39
0.234ProHis: 0.234 ± 0.135
2.267ProIle: 2.267 ± 0.526
2.345ProLys: 2.345 ± 0.514
2.892ProLeu: 2.892 ± 0.386
0.469ProMet: 0.469 ± 0.226
1.876ProAsn: 1.876 ± 0.561
1.719ProPro: 1.719 ± 0.744
1.798ProGln: 1.798 ± 0.398
1.094ProArg: 1.094 ± 0.223
2.11ProSer: 2.11 ± 0.3
2.579ProThr: 2.579 ± 0.792
2.188ProVal: 2.188 ± 0.405
0.313ProTrp: 0.313 ± 0.145
1.485ProTyr: 1.485 ± 0.42
0.0ProXaa: 0.0 ± 0.0
Gln
4.299GlnAla: 4.299 ± 0.627
0.078GlnCys: 0.078 ± 0.104
2.11GlnAsp: 2.11 ± 0.527
4.142GlnGlu: 4.142 ± 0.694
2.345GlnPhe: 2.345 ± 0.741
3.204GlnGly: 3.204 ± 0.645
0.469GlnHis: 0.469 ± 0.172
2.188GlnIle: 2.188 ± 0.407
3.361GlnLys: 3.361 ± 0.81
3.908GlnLeu: 3.908 ± 0.583
1.016GlnMet: 1.016 ± 0.355
2.501GlnAsn: 2.501 ± 0.667
1.563GlnPro: 1.563 ± 0.397
4.377GlnGln: 4.377 ± 1.577
2.501GlnArg: 2.501 ± 0.701
2.188GlnSer: 2.188 ± 0.379
2.267GlnThr: 2.267 ± 0.402
2.267GlnVal: 2.267 ± 0.445
1.25GlnTrp: 1.25 ± 0.625
1.329GlnTyr: 1.329 ± 0.473
0.0GlnXaa: 0.0 ± 0.0
Arg
3.439ArgAla: 3.439 ± 0.641
0.547ArgCys: 0.547 ± 0.212
2.345ArgAsp: 2.345 ± 0.495
3.439ArgGlu: 3.439 ± 0.625
1.954ArgPhe: 1.954 ± 0.387
2.345ArgGly: 2.345 ± 0.684
1.016ArgHis: 1.016 ± 0.374
2.501ArgIle: 2.501 ± 0.349
3.595ArgLys: 3.595 ± 0.441
3.986ArgLeu: 3.986 ± 0.495
1.094ArgMet: 1.094 ± 0.254
1.954ArgAsn: 1.954 ± 0.391
0.547ArgPro: 0.547 ± 0.2
2.11ArgGln: 2.11 ± 0.554
1.641ArgArg: 1.641 ± 0.394
2.188ArgSer: 2.188 ± 0.419
2.11ArgThr: 2.11 ± 0.354
2.814ArgVal: 2.814 ± 0.37
0.234ArgTrp: 0.234 ± 0.122
1.876ArgTyr: 1.876 ± 0.334
0.0ArgXaa: 0.0 ± 0.0
Ser
3.517SerAla: 3.517 ± 0.519
0.156SerCys: 0.156 ± 0.111
3.126SerAsp: 3.126 ± 0.553
3.048SerGlu: 3.048 ± 0.349
2.892SerPhe: 2.892 ± 0.485
4.924SerGly: 4.924 ± 0.849
1.094SerHis: 1.094 ± 0.299
3.751SerIle: 3.751 ± 0.476
4.377SerLys: 4.377 ± 0.454
4.299SerLeu: 4.299 ± 0.535
1.407SerMet: 1.407 ± 0.429
3.204SerAsn: 3.204 ± 0.489
2.579SerPro: 2.579 ± 0.458
3.204SerGln: 3.204 ± 0.777
1.563SerArg: 1.563 ± 0.405
4.689SerSer: 4.689 ± 0.823
2.814SerThr: 2.814 ± 0.611
2.97SerVal: 2.97 ± 0.445
0.625SerTrp: 0.625 ± 0.179
2.657SerTyr: 2.657 ± 0.505
0.0SerXaa: 0.0 ± 0.0
Thr
5.393ThrAla: 5.393 ± 0.801
0.313ThrCys: 0.313 ± 0.173
2.501ThrAsp: 2.501 ± 0.435
3.751ThrGlu: 3.751 ± 0.481
2.423ThrPhe: 2.423 ± 0.494
4.22ThrGly: 4.22 ± 0.591
1.016ThrHis: 1.016 ± 0.393
4.455ThrIle: 4.455 ± 0.816
4.846ThrLys: 4.846 ± 0.519
3.986ThrLeu: 3.986 ± 0.439
1.016ThrMet: 1.016 ± 0.336
3.986ThrAsn: 3.986 ± 0.505
3.517ThrPro: 3.517 ± 0.607
2.501ThrGln: 2.501 ± 0.416
1.485ThrArg: 1.485 ± 0.25
3.673ThrSer: 3.673 ± 0.682
3.048ThrThr: 3.048 ± 0.428
4.142ThrVal: 4.142 ± 0.973
0.703ThrTrp: 0.703 ± 0.296
2.814ThrTyr: 2.814 ± 0.524
0.0ThrXaa: 0.0 ± 0.0
Val
5.08ValAla: 5.08 ± 0.636
0.313ValCys: 0.313 ± 0.174
4.611ValAsp: 4.611 ± 0.459
4.064ValGlu: 4.064 ± 0.602
2.188ValPhe: 2.188 ± 0.432
4.377ValGly: 4.377 ± 0.668
1.094ValHis: 1.094 ± 0.255
3.595ValIle: 3.595 ± 0.703
4.689ValLys: 4.689 ± 0.513
4.299ValLeu: 4.299 ± 0.68
1.798ValMet: 1.798 ± 0.362
3.361ValAsn: 3.361 ± 0.708
2.345ValPro: 2.345 ± 0.323
2.188ValGln: 2.188 ± 0.39
2.345ValArg: 2.345 ± 0.446
4.455ValSer: 4.455 ± 0.7
3.83ValThr: 3.83 ± 0.469
2.657ValVal: 2.657 ± 0.554
0.313ValTrp: 0.313 ± 0.16
2.188ValTyr: 2.188 ± 0.537
0.0ValXaa: 0.0 ± 0.0
Trp
0.782TrpAla: 0.782 ± 0.283
0.078TrpCys: 0.078 ± 0.076
0.391TrpAsp: 0.391 ± 0.18
0.86TrpGlu: 0.86 ± 0.311
0.234TrpPhe: 0.234 ± 0.139
0.625TrpGly: 0.625 ± 0.223
0.078TrpHis: 0.078 ± 0.071
0.469TrpIle: 0.469 ± 0.221
1.719TrpLys: 1.719 ± 0.439
1.094TrpLeu: 1.094 ± 0.269
0.234TrpMet: 0.234 ± 0.125
1.094TrpAsn: 1.094 ± 0.268
0.234TrpPro: 0.234 ± 0.157
1.016TrpGln: 1.016 ± 0.61
0.625TrpArg: 0.625 ± 0.276
0.469TrpSer: 0.469 ± 0.212
0.469TrpThr: 0.469 ± 0.196
0.782TrpVal: 0.782 ± 0.282
0.078TrpTrp: 0.078 ± 0.071
0.625TrpTyr: 0.625 ± 0.213
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.11TyrAla: 2.11 ± 0.288
0.234TyrCys: 0.234 ± 0.173
2.657TyrAsp: 2.657 ± 0.401
2.345TyrGlu: 2.345 ± 0.464
1.876TyrPhe: 1.876 ± 0.563
2.501TyrGly: 2.501 ± 0.543
0.782TyrHis: 0.782 ± 0.245
1.954TyrIle: 1.954 ± 0.476
4.22TyrLys: 4.22 ± 0.585
2.579TyrLeu: 2.579 ± 0.409
0.86TyrMet: 0.86 ± 0.287
2.579TyrAsn: 2.579 ± 0.496
1.641TyrPro: 1.641 ± 0.403
1.641TyrGln: 1.641 ± 0.35
1.954TyrArg: 1.954 ± 0.316
2.188TyrSer: 2.188 ± 0.41
2.267TyrThr: 2.267 ± 0.419
2.735TyrVal: 2.735 ± 0.539
0.782TyrTrp: 0.782 ± 0.207
1.485TyrTyr: 1.485 ± 0.436
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 50 proteins (12796 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski