Pectobacterium phage DU_PP_IV

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Vequintavirinae; Certrevirus; unclassified Certrevirus

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 268 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2D2W6P9|A0A2D2W6P9_9CAUD Uncharacterized protein OS=Pectobacterium phage DU_PP_IV OX=2041491 GN=P12B145kb_p168 PE=4 SV=1
MM1 pKa = 7.42GKK3 pKa = 9.5SLSEE7 pKa = 3.76KK8 pKa = 9.85WLDD11 pKa = 3.79AGIDD15 pKa = 3.39KK16 pKa = 10.7CLNPPVEE23 pKa = 4.39EE24 pKa = 4.34TEE26 pKa = 4.63DD27 pKa = 3.45MLIMVDD33 pKa = 4.64EE34 pKa = 4.59EE35 pKa = 4.42PEE37 pKa = 3.87YY38 pKa = 10.84DD39 pKa = 3.86SPHH42 pKa = 5.93GSVWIVVVDD51 pKa = 3.2YY52 pKa = 10.99AGRR55 pKa = 11.84VTVLDD60 pKa = 4.51PPNLHH65 pKa = 6.96PGIMDD70 pKa = 4.49NGPEE74 pKa = 3.92AEE76 pKa = 4.33YY77 pKa = 10.86LGLPPDD83 pKa = 4.36VDD85 pKa = 4.74DD86 pKa = 4.97MDD88 pKa = 4.96PGVYY92 pKa = 9.04RR93 pKa = 11.84WTCSYY98 pKa = 8.49HH99 pKa = 3.88THH101 pKa = 6.75IDD103 pKa = 3.65RR104 pKa = 11.84EE105 pKa = 4.4SGHH108 pKa = 6.88VDD110 pKa = 3.32DD111 pKa = 5.82YY112 pKa = 11.87DD113 pKa = 4.28FEE115 pKa = 5.34IEE117 pKa = 4.2EE118 pKa = 4.79SVLLWSPDD126 pKa = 3.28NAA128 pKa = 4.28

Molecular weight: 14.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2D2W6U5|A0A2D2W6U5_9CAUD HNH endonuclease OS=Pectobacterium phage DU_PP_IV OX=2041491 GN=P12B145kb_p227 PE=4 SV=1
MM1 pKa = 7.06TVLWWFIYY9 pKa = 9.99RR10 pKa = 11.84RR11 pKa = 11.84WRR13 pKa = 11.84MISWTKK19 pKa = 10.95DD20 pKa = 2.76YY21 pKa = 11.36DD22 pKa = 3.45AGMKK26 pKa = 10.03KK27 pKa = 10.24DD28 pKa = 3.58VLNNGYY34 pKa = 9.53RR35 pKa = 11.84VNLCSCRR42 pKa = 11.84EE43 pKa = 3.63HH44 pKa = 6.62RR45 pKa = 11.84AIYY48 pKa = 10.36RR49 pKa = 11.84DD50 pKa = 3.3MQRR53 pKa = 11.84ILKK56 pKa = 9.96

Molecular weight: 7.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

268

0

268

43498

38

1088

162.3

18.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.256 ± 0.186

1.361 ± 0.092

6.55 ± 0.136

6.98 ± 0.174

4.237 ± 0.126

7.819 ± 0.298

1.982 ± 0.105

5.55 ± 0.103

6.784 ± 0.167

7.846 ± 0.165

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.892 ± 0.096

4.398 ± 0.097

3.927 ± 0.128

3.423 ± 0.133

5.455 ± 0.158

5.414 ± 0.138

5.513 ± 0.183

7.104 ± 0.141

1.671 ± 0.075

3.839 ± 0.109
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski