Agitococcus lubricus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Moraxellaceae; Agitococcus

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3309 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T5IVN4|A0A2T5IVN4_9GAMM Lipoyl synthase OS=Agitococcus lubricus OX=1077255 GN=lipA PE=3 SV=1
MM1 pKa = 6.92NTQIQAIFDD10 pKa = 3.84HH11 pKa = 6.99ALLANAVYY19 pKa = 10.82KK20 pKa = 10.85NLTANTTPEE29 pKa = 3.9VFAASLTGWPPALATYY45 pKa = 10.64LSTRR49 pKa = 11.84YY50 pKa = 9.88KK51 pKa = 10.67VVEE54 pKa = 4.43TYY56 pKa = 11.84NMGDD60 pKa = 3.13NVSGYY65 pKa = 10.79NGALFVGIVEE75 pKa = 4.47NDD77 pKa = 3.29NQNKK81 pKa = 10.32YY82 pKa = 10.15IFANRR87 pKa = 11.84GTEE90 pKa = 3.92PTSPSDD96 pKa = 3.97LFADD100 pKa = 4.31LLLAISGANQQLPVMQAYY118 pKa = 9.69FEE120 pKa = 4.14QALVKK125 pKa = 10.99VNGNLLTVTGHH136 pKa = 6.11SLGGYY141 pKa = 9.84LSVVLQNPDD150 pKa = 3.04EE151 pKa = 4.34NGIFIDD157 pKa = 3.82KK158 pKa = 10.18TYY160 pKa = 9.56TFNGAGIFNVALTGSIVNAGLVALGYY186 pKa = 10.6EE187 pKa = 5.05PITVSPSLPIEE198 pKa = 4.01NYY200 pKa = 8.31YY201 pKa = 9.33TVEE204 pKa = 4.01GLTVTSATGVYY215 pKa = 9.59GGVRR219 pKa = 11.84IPVNTDD225 pKa = 2.97VIGLGGVGGLEE236 pKa = 3.91GHH238 pKa = 6.81SIANLTDD245 pKa = 3.43ALAVYY250 pKa = 10.65NLMGMVDD257 pKa = 3.56SDD259 pKa = 3.46ITLAEE264 pKa = 4.11LNGIHH269 pKa = 6.42EE270 pKa = 4.95AMSKK274 pKa = 10.82DD275 pKa = 3.38GLIEE279 pKa = 3.86NDD281 pKa = 3.39ALVKK285 pKa = 10.79VFGTLLTKK293 pKa = 10.52SLTIFQQTTKK303 pKa = 11.23DD304 pKa = 4.08KK305 pKa = 11.39VDD307 pKa = 4.2LLNVSANNNNAMHH320 pKa = 6.17EE321 pKa = 4.47AIYY324 pKa = 11.3AMVNDD329 pKa = 5.15LATTAQALKK338 pKa = 10.19IIPLVSFTNQGEE350 pKa = 4.25LQLFNAQIPNNIAYY364 pKa = 9.86RR365 pKa = 11.84YY366 pKa = 8.96ALQVLNPFFVEE377 pKa = 4.57SSDD380 pKa = 3.82SANTEE385 pKa = 3.27TMYY388 pKa = 11.0QVFNEE393 pKa = 4.02HH394 pKa = 6.43QEE396 pKa = 4.0LDD398 pKa = 4.06LYY400 pKa = 11.4DD401 pKa = 5.04ADD403 pKa = 4.34TEE405 pKa = 4.38KK406 pKa = 10.83GTLTLEE412 pKa = 4.13WLEE415 pKa = 4.55KK416 pKa = 9.47RR417 pKa = 11.84HH418 pKa = 5.97EE419 pKa = 4.09MLNTQIKK426 pKa = 10.86ANIQNRR432 pKa = 11.84GFMTHH437 pKa = 5.92EE438 pKa = 4.2GEE440 pKa = 3.98YY441 pKa = 10.26NYY443 pKa = 10.82YY444 pKa = 10.21YY445 pKa = 10.59EE446 pKa = 5.91AIINNEE452 pKa = 3.74RR453 pKa = 11.84VIVKK457 pKa = 10.4DD458 pKa = 3.13MSTTSRR464 pKa = 11.84SLNDD468 pKa = 3.65PDD470 pKa = 4.01SPMVEE475 pKa = 3.81KK476 pKa = 10.08TRR478 pKa = 11.84YY479 pKa = 8.1TKK481 pKa = 10.6FADD484 pKa = 3.45KK485 pKa = 11.21NNANFTGGDD494 pKa = 3.54DD495 pKa = 3.54KK496 pKa = 11.65DD497 pKa = 3.83YY498 pKa = 11.6LFGHH502 pKa = 6.25QGNDD506 pKa = 2.84VLYY509 pKa = 10.19GLEE512 pKa = 4.11EE513 pKa = 3.87EE514 pKa = 5.51DD515 pKa = 3.76YY516 pKa = 11.63LEE518 pKa = 4.87GGEE521 pKa = 5.54GDD523 pKa = 3.74DD524 pKa = 4.18TLDD527 pKa = 3.64GGEE530 pKa = 4.39GRR532 pKa = 11.84DD533 pKa = 3.39ILVGMTGDD541 pKa = 5.03DD542 pKa = 3.71SLKK545 pKa = 10.57GDD547 pKa = 4.09KK548 pKa = 11.36GNDD551 pKa = 3.54TLEE554 pKa = 4.46GGAGKK559 pKa = 8.6DD560 pKa = 3.46TLDD563 pKa = 4.28GGEE566 pKa = 4.69DD567 pKa = 3.35HH568 pKa = 7.66DD569 pKa = 4.95KK570 pKa = 10.99LYY572 pKa = 11.21SHH574 pKa = 6.99SGDD577 pKa = 3.56DD578 pKa = 3.66TLKK581 pKa = 11.16GGLGDD586 pKa = 5.02DD587 pKa = 4.33LLDD590 pKa = 4.16GGKK593 pKa = 10.55EE594 pKa = 3.84NDD596 pKa = 4.24TLDD599 pKa = 4.26GDD601 pKa = 4.33DD602 pKa = 5.24GNDD605 pKa = 3.1ILLGQLGDD613 pKa = 5.09DD614 pKa = 3.96ILHH617 pKa = 6.28GGKK620 pKa = 10.8GNDD623 pKa = 3.38YY624 pKa = 10.58LYY626 pKa = 10.99GGEE629 pKa = 4.93GEE631 pKa = 5.2DD632 pKa = 4.06EE633 pKa = 4.55LFGEE637 pKa = 5.0AGNDD641 pKa = 3.85LIVGGKK647 pKa = 10.34GILTTAKK654 pKa = 10.27GGEE657 pKa = 4.28GNDD660 pKa = 4.54LIFSDD665 pKa = 4.59TVWAYY670 pKa = 10.11EE671 pKa = 3.8DD672 pKa = 3.82TLIGEE677 pKa = 4.45SGNDD681 pKa = 3.07YY682 pKa = 10.65LAAYY686 pKa = 9.09GGNDD690 pKa = 3.1ILKK693 pKa = 10.5GEE695 pKa = 4.54AGNDD699 pKa = 3.33YY700 pKa = 11.2LLGYY704 pKa = 8.42TGNDD708 pKa = 3.4SLLGGADD715 pKa = 3.36NDD717 pKa = 3.78VVQAGEE723 pKa = 4.21GDD725 pKa = 3.68DD726 pKa = 3.74TLVGGLHH733 pKa = 6.9HH734 pKa = 7.52DD735 pKa = 4.48LLRR738 pKa = 11.84GGNGNDD744 pKa = 3.35TYY746 pKa = 11.48SYY748 pKa = 9.18TQLEE752 pKa = 4.49GVDD755 pKa = 5.0LIEE758 pKa = 5.88DD759 pKa = 3.61SDD761 pKa = 4.14GKK763 pKa = 10.72IVIQNVTLAVTDD775 pKa = 3.84YY776 pKa = 11.64NNEE779 pKa = 3.83KK780 pKa = 10.66KK781 pKa = 10.22LWQSNTGQEE790 pKa = 3.83IRR792 pKa = 11.84RR793 pKa = 11.84LSNEE797 pKa = 3.93EE798 pKa = 4.05GNLTTVLIGQHH809 pKa = 5.63NNQIVISNFQQSQFGLTFSGGEE831 pKa = 3.82QEE833 pKa = 4.97RR834 pKa = 11.84VINVSDD840 pKa = 3.94PAKK843 pKa = 10.69QGTNQNNIIEE853 pKa = 4.54ISNHH857 pKa = 4.46VYY859 pKa = 10.41GLEE862 pKa = 4.32GNDD865 pKa = 3.45YY866 pKa = 11.2LYY868 pKa = 10.26GTVNNDD874 pKa = 2.93KK875 pKa = 10.78LYY877 pKa = 11.23GGIGGDD883 pKa = 3.71FLIGLSGDD891 pKa = 4.32DD892 pKa = 3.92YY893 pKa = 11.7IEE895 pKa = 4.78GNGDD899 pKa = 3.5DD900 pKa = 5.63DD901 pKa = 6.01IIFGGDD907 pKa = 3.53GDD909 pKa = 5.24DD910 pKa = 4.25ILYY913 pKa = 10.65GDD915 pKa = 4.81SGDD918 pKa = 4.61DD919 pKa = 3.88VIFSALKK926 pKa = 10.19IPVSFYY932 pKa = 11.1SWGLDD937 pKa = 2.97RR938 pKa = 11.84KK939 pKa = 9.97IPLSSIHH946 pKa = 5.55TQNPAEE952 pKa = 4.11GQFADD957 pKa = 3.66EE958 pKa = 5.11FDD960 pKa = 4.19NISYY964 pKa = 10.3KK965 pKa = 9.95IEE967 pKa = 3.87RR968 pKa = 11.84VLDD971 pKa = 3.89TEE973 pKa = 4.27NEE975 pKa = 4.27SYY977 pKa = 10.47TIYY980 pKa = 10.82FNGIDD985 pKa = 3.73EE986 pKa = 4.74NLDD989 pKa = 3.17VLLPVIKK996 pKa = 9.82IYY998 pKa = 11.32AEE1000 pKa = 3.85ISGEE1004 pKa = 4.01KK1005 pKa = 9.78YY1006 pKa = 9.59MLYY1009 pKa = 9.0STYY1012 pKa = 10.68SPEE1015 pKa = 4.23VYY1017 pKa = 10.35FEE1019 pKa = 4.31LTDD1022 pKa = 3.54EE1023 pKa = 4.25YY1024 pKa = 11.43NYY1026 pKa = 10.72FLDD1029 pKa = 4.34LRR1031 pKa = 11.84YY1032 pKa = 10.34EE1033 pKa = 4.16GLINNDD1039 pKa = 3.29DD1040 pKa = 4.04VYY1042 pKa = 11.5GGDD1045 pKa = 4.28GDD1047 pKa = 5.0DD1048 pKa = 4.44FLIGSGGIDD1057 pKa = 3.42KK1058 pKa = 10.63IYY1060 pKa = 10.65GNKK1063 pKa = 10.41DD1064 pKa = 2.6NDD1066 pKa = 3.55YY1067 pKa = 10.7LYY1069 pKa = 11.34GRR1071 pKa = 11.84GGDD1074 pKa = 3.55DD1075 pKa = 3.16YY1076 pKa = 11.77LYY1078 pKa = 11.11GNEE1081 pKa = 4.38GKK1083 pKa = 10.29DD1084 pKa = 3.49EE1085 pKa = 4.09IFGGDD1090 pKa = 4.17GIDD1093 pKa = 4.96LIDD1096 pKa = 5.27GGLEE1100 pKa = 3.73NDD1102 pKa = 3.89KK1103 pKa = 11.19LYY1105 pKa = 11.21GGYY1108 pKa = 10.34EE1109 pKa = 3.98SDD1111 pKa = 3.43VLYY1114 pKa = 11.23GGMGDD1119 pKa = 4.84DD1120 pKa = 4.9LVLGDD1125 pKa = 4.81FPNLYY1130 pKa = 8.86GTTTPPDD1137 pKa = 4.08SINSSRR1143 pKa = 11.84YY1144 pKa = 9.25GHH1146 pKa = 7.43DD1147 pKa = 2.54ILYY1150 pKa = 10.57GEE1152 pKa = 5.09KK1153 pKa = 11.12GNDD1156 pKa = 3.22TLFGGGADD1164 pKa = 3.76DD1165 pKa = 3.75YY1166 pKa = 11.65LYY1168 pKa = 11.28GNEE1171 pKa = 4.67DD1172 pKa = 3.86DD1173 pKa = 5.58DD1174 pKa = 4.66VVVGNEE1180 pKa = 3.8GNDD1183 pKa = 3.39YY1184 pKa = 11.29LFGGKK1189 pKa = 10.0DD1190 pKa = 3.2NDD1192 pKa = 3.43ILYY1195 pKa = 10.62GDD1197 pKa = 3.78SHH1199 pKa = 6.18EE1200 pKa = 4.19QSEE1203 pKa = 4.37AAEE1206 pKa = 4.39GNDD1209 pKa = 3.27YY1210 pKa = 11.56LEE1212 pKa = 4.73GGEE1215 pKa = 4.87GKK1217 pKa = 8.5DD1218 pKa = 3.22TLFGNGGDD1226 pKa = 3.9DD1227 pKa = 3.47VLMGGQEE1234 pKa = 3.99RR1235 pKa = 11.84DD1236 pKa = 3.39EE1237 pKa = 5.65LYY1239 pKa = 10.94DD1240 pKa = 3.84PSQLNGQLFPLKK1252 pKa = 10.63LII1254 pKa = 4.38

Molecular weight: 135.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T5J1U5|A0A2T5J1U5_9GAMM Magnesium and cobalt transporter OS=Agitococcus lubricus OX=1077255 GN=C8N29_103168 PE=4 SV=1
MM1 pKa = 7.1SAWVLFIACLVICLRR16 pKa = 11.84LFTFQRR22 pKa = 11.84KK23 pKa = 6.24GARR26 pKa = 11.84YY27 pKa = 8.87RR28 pKa = 11.84PVMSFGAYY36 pKa = 10.47LLMVFSFSIMVKK48 pKa = 10.26LALNQFPCHH57 pKa = 6.28VSPFMSLAAVGLAWLTLVNKK77 pKa = 10.42GNVAHH82 pKa = 7.07FFRR85 pKa = 11.84KK86 pKa = 9.5HH87 pKa = 3.69SHH89 pKa = 5.13GG90 pKa = 3.63

Molecular weight: 10.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3309

0

3309

1093339

26

4622

330.4

36.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.579 ± 0.053

0.987 ± 0.015

5.303 ± 0.04

5.43 ± 0.042

3.789 ± 0.03

6.646 ± 0.059

2.435 ± 0.027

6.054 ± 0.031

4.871 ± 0.046

10.919 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.368 ± 0.026

4.21 ± 0.059

4.076 ± 0.037

5.443 ± 0.049

4.898 ± 0.044

6.065 ± 0.041

5.688 ± 0.078

6.817 ± 0.037

1.332 ± 0.02

3.088 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski