Synechococcus sp. (strain WH7805)
Average proteome isoelectric point is 6.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2874 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A4CWW8|A4CWW8_SYNPV Elp3 domain-containing protein OS=Synechococcus sp. (strain WH7805) OX=59931 GN=WH7805_09569 PE=4 SV=1
MM1 pKa = 7.3 NLSNLIEE8 pKa = 4.32 SLSAYY13 pKa = 9.62 RR14 pKa = 11.84 ARR16 pKa = 11.84 SLHH19 pKa = 5.77 TSLNQPEE26 pKa = 4.82 EE27 pKa = 4.37 PDD29 pKa = 3.83 PDD31 pKa = 3.47 ISSIDD36 pKa = 3.73 NNPALTPIDD45 pKa = 3.51 QPLTVARR52 pKa = 11.84 GLLQNGATTPPSQQLNSTEE71 pKa = 4.28 SFSEE75 pKa = 4.52 AINGNNSITTEE86 pKa = 4.03 EE87 pKa = 4.3 EE88 pKa = 3.87 ANEE91 pKa = 4.0 TTNIEE96 pKa = 3.8 FRR98 pKa = 11.84 IEE100 pKa = 4.09 SKK102 pKa = 10.51 YY103 pKa = 10.99 LHH105 pKa = 7.01 AGRR108 pKa = 11.84 CACFACSYY116 pKa = 10.94 SRR118 pKa = 11.84 DD119 pKa = 3.56 SLEE122 pKa = 4.11 TNRR125 pKa = 11.84 EE126 pKa = 4.25 SNSEE130 pKa = 4.1 LNNTLSNADD139 pKa = 3.38 PTIAFGTLNEE149 pKa = 4.15 LSDD152 pKa = 4.01 YY153 pKa = 9.5 LTTGFWEE160 pKa = 4.19 EE161 pKa = 3.61 AGTYY165 pKa = 7.19 TRR167 pKa = 11.84 RR168 pKa = 11.84 FNLGGSGLGAKK179 pKa = 9.84 NGQLTYY185 pKa = 10.99 NITGWADD192 pKa = 3.34 DD193 pKa = 4.12 SNGLSAEE200 pKa = 4.16 RR201 pKa = 11.84 QNLTRR206 pKa = 11.84 EE207 pKa = 4.05 VFNVYY212 pKa = 9.39 EE213 pKa = 5.0 AITGIEE219 pKa = 4.12 FVEE222 pKa = 4.71 VATEE226 pKa = 3.88 GDD228 pKa = 4.31 FRR230 pKa = 11.84 FTDD233 pKa = 3.74 NDD235 pKa = 3.64 SGAYY239 pKa = 9.83 AYY241 pKa = 10.81 LGGGWYY247 pKa = 9.94 DD248 pKa = 3.29 QDD250 pKa = 4.71 PNTGTIDD257 pKa = 3.46 YY258 pKa = 9.12 NAAIIDD264 pKa = 3.69 YY265 pKa = 10.57 SVINVASSWFYY276 pKa = 11.49 GDD278 pKa = 3.68 SSYY281 pKa = 11.9 NSYY284 pKa = 9.32 TPQTIFHH291 pKa = 7.24 EE292 pKa = 4.72 IGHH295 pKa = 6.51 ALGLGHH301 pKa = 6.58 QGQYY305 pKa = 10.39 NAGNGNPTYY314 pKa = 10.38 EE315 pKa = 4.43 NSAQYY320 pKa = 11.53 GNDD323 pKa = 2.93 TWLTTMMSYY332 pKa = 9.87 WSQTTNTNVNASRR345 pKa = 11.84 AYY347 pKa = 10.07 LQTPMTVDD355 pKa = 3.9 WIALDD360 pKa = 3.81 KK361 pKa = 11.2 LYY363 pKa = 11.05 GSQGYY368 pKa = 10.19 GSFKK372 pKa = 10.64 AFNGNTVYY380 pKa = 10.78 GVGTNISSGTSEE392 pKa = 3.97 IMNNFATMISDD403 pKa = 3.9 TAYY406 pKa = 9.87 TLVDD410 pKa = 3.66 GSGYY414 pKa = 10.79 DD415 pKa = 3.2 ILNVSNYY422 pKa = 10.35 SDD424 pKa = 3.48 DD425 pKa = 3.68 QFINLAPSEE434 pKa = 4.31 LNGTLPSSSSIGGLVNNLTIGVGTILEE461 pKa = 4.17 EE462 pKa = 4.95 AIGGSGNDD470 pKa = 3.29 TFLGNIADD478 pKa = 3.8 NVFRR482 pKa = 11.84 GGNGGDD488 pKa = 3.07 RR489 pKa = 11.84 FYY491 pKa = 11.54 DD492 pKa = 3.5 SFGSDD497 pKa = 2.67 TYY499 pKa = 11.41 YY500 pKa = 11.06 GGSGTFDD507 pKa = 2.76 TLYY510 pKa = 10.81 FSEE513 pKa = 4.75 NYY515 pKa = 10.3 SDD517 pKa = 5.56 FSIEE521 pKa = 4.03 NLGTSLAFSRR531 pKa = 11.84 NNSGLADD538 pKa = 4.04 TDD540 pKa = 5.5 LIWNDD545 pKa = 2.73 IEE547 pKa = 5.2 LVYY550 pKa = 11.06 FNDD553 pKa = 4.72 DD554 pKa = 3.55 VIKK557 pKa = 9.85 TYY559 pKa = 10.83 QEE561 pKa = 3.9 LLADD565 pKa = 3.95 ITPINTPPTANDD577 pKa = 3.1 VFVATSEE584 pKa = 4.37 DD585 pKa = 4.05 SNSVSGNFNANDD597 pKa = 4.43 PDD599 pKa = 4.47 SSDD602 pKa = 3.1 QLTYY606 pKa = 10.73 TITTAPTIGSVVVNSDD622 pKa = 3.12 GTFTYY627 pKa = 11.06 NFGNNFQSLGDD638 pKa = 3.87 GDD640 pKa = 3.92 STAVVFQYY648 pKa = 10.52 IASDD652 pKa = 3.99 DD653 pKa = 4.09 NATDD657 pKa = 3.68 SAPATVTITINGSNDD672 pKa = 2.91 APILSGTPSALTNGTEE688 pKa = 4.67 DD689 pKa = 3.05 IAYY692 pKa = 10.01 LLKK695 pKa = 10.96 ASDD698 pKa = 4.24 LLQGYY703 pKa = 9.81 SDD705 pKa = 3.89 IDD707 pKa = 3.94 GDD709 pKa = 4.18 TLSITSVTTASANGTLTPNGNGTWTYY735 pKa = 11.08 TPTQDD740 pKa = 4.41 LNGEE744 pKa = 4.46 VVFSFVVSDD753 pKa = 4.19 GNGGTANGSTSLTLDD768 pKa = 3.49 PVEE771 pKa = 5.89 DD772 pKa = 3.84 EE773 pKa = 4.64 VLLDD777 pKa = 3.78 EE778 pKa = 5.44 DD779 pKa = 4.05 NNGIVDD785 pKa = 4.06 GTEE788 pKa = 3.5 LTAYY792 pKa = 10.44 QLFSEE797 pKa = 5.14 AGAITLKK804 pKa = 11.0 NNAGQTYY811 pKa = 9.93 NNSSSGNSDD820 pKa = 2.9 VVAAIEE826 pKa = 4.21 TDD828 pKa = 2.99 NGFQVLLEE836 pKa = 4.3 GTGSRR841 pKa = 11.84 NDD843 pKa = 3.39 TFYY846 pKa = 11.64 VWNTNSNGVIIGGSGWKK863 pKa = 10.22 SGDD866 pKa = 2.84 IATRR870 pKa = 11.84 LGWEE874 pKa = 3.9 EE875 pKa = 4.07 TFNFDD880 pKa = 3.69 TNRR883 pKa = 11.84 DD884 pKa = 3.52 GTIGALILDD893 pKa = 4.83 DD894 pKa = 5.09 DD895 pKa = 4.67 NNGIVDD901 pKa = 4.05 GTEE904 pKa = 3.5 LTAYY908 pKa = 10.44 QLFSEE913 pKa = 5.14 AGAITLKK920 pKa = 11.0 NNAGQTYY927 pKa = 9.93 NNSSSGNSDD936 pKa = 2.9 VVAAIEE942 pKa = 4.21 TDD944 pKa = 2.99 NGFQVLLEE952 pKa = 4.3 GTGSRR957 pKa = 11.84 NDD959 pKa = 3.39 TFYY962 pKa = 11.64 VWNTNSNGVIIGGSGWKK979 pKa = 10.22 SGDD982 pKa = 2.84 IATRR986 pKa = 11.84 LGWEE990 pKa = 3.9 EE991 pKa = 4.07 TFNFDD996 pKa = 3.69 TNRR999 pKa = 11.84 DD1000 pKa = 3.52 GTIGALILDD1009 pKa = 4.83 DD1010 pKa = 5.09 DD1011 pKa = 4.67 NNGIVDD1017 pKa = 4.05 GTEE1020 pKa = 3.5 LTAYY1024 pKa = 10.44 QLFSEE1029 pKa = 5.14 AGAITLKK1036 pKa = 11.0 NNAGQTYY1043 pKa = 9.93 NNSSSGNSDD1052 pKa = 2.9 VVAAIEE1058 pKa = 4.21 TDD1060 pKa = 2.99 NGFQVLLEE1068 pKa = 4.3 GTGSRR1073 pKa = 11.84 NDD1075 pKa = 3.39 TFYY1078 pKa = 11.64 VWNTNSNGVITGGSGWKK1095 pKa = 10.22 SGDD1098 pKa = 2.84 IATRR1102 pKa = 11.84 LGWEE1106 pKa = 3.9 EE1107 pKa = 4.07 TFNFDD1112 pKa = 3.69 TNRR1115 pKa = 11.84 DD1116 pKa = 3.52 GTIGALILDD1125 pKa = 4.83 DD1126 pKa = 5.09 DD1127 pKa = 4.67 NNGIVDD1133 pKa = 4.05 GTEE1136 pKa = 3.5 LTAYY1140 pKa = 10.44 QLFSEE1145 pKa = 5.14 AGAITLKK1152 pKa = 11.0 NNAGQTYY1159 pKa = 9.93 NNSSSGNSDD1168 pKa = 2.9 VVAAIEE1174 pKa = 4.21 TDD1176 pKa = 2.99 NGFQVLLEE1184 pKa = 4.3 GTGSRR1189 pKa = 11.84 NDD1191 pKa = 3.39 TFYY1194 pKa = 11.64 VWNTNSNGVITGGSGWKK1211 pKa = 10.22 SGDD1214 pKa = 2.84 IATRR1218 pKa = 11.84 LGWEE1222 pKa = 3.9 EE1223 pKa = 4.07 TFNFDD1228 pKa = 3.69 TNRR1231 pKa = 11.84 DD1232 pKa = 3.52 GTIGALILDD1241 pKa = 4.83 DD1242 pKa = 5.09 DD1243 pKa = 4.67 NNGIVDD1249 pKa = 4.05 GTEE1252 pKa = 3.5 LTAYY1256 pKa = 10.44 QLFSEE1261 pKa = 5.14 AGAITLKK1268 pKa = 11.0 NNAGQTYY1275 pKa = 9.93 NNSSSGNSDD1284 pKa = 2.9 VVAAIEE1290 pKa = 4.21 TDD1292 pKa = 2.99 NGFQVLLEE1300 pKa = 4.3 GTGSRR1305 pKa = 11.84 NDD1307 pKa = 3.39 TFYY1310 pKa = 11.62 VWDD1313 pKa = 3.94 TNSNGVITGGSGWKK1327 pKa = 9.67 SGNIATRR1334 pKa = 11.84 LGWEE1338 pKa = 3.9 EE1339 pKa = 4.07 TFNFDD1344 pKa = 3.69 TNRR1347 pKa = 11.84 DD1348 pKa = 3.61 GTIGLPPSLSSPTLEE1363 pKa = 5.49 ARR1365 pKa = 11.84 FKK1367 pKa = 10.78 DD1368 pKa = 3.47 QWHH1371 pKa = 6.68 LKK1373 pKa = 10.03 DD1374 pKa = 4.03 SSSGGANVASAWLLKK1389 pKa = 10.56 NKK1391 pKa = 10.1 SGSNIYY1397 pKa = 8.77 GTGIHH1402 pKa = 6.59 INVIDD1407 pKa = 5.64 DD1408 pKa = 3.98 GLDD1411 pKa = 3.03 WRR1413 pKa = 11.84 HH1414 pKa = 5.74 QDD1416 pKa = 3.59 LSTNYY1421 pKa = 9.32 ISASSYY1427 pKa = 11.41 DD1428 pKa = 3.95 YY1429 pKa = 11.14 VGKK1432 pKa = 10.56 DD1433 pKa = 3.32 NDD1435 pKa = 3.98 PTPSSSADD1443 pKa = 3.0 HH1444 pKa = 5.9 GTAVAGVAAGYY1455 pKa = 8.67 GHH1457 pKa = 6.88 NGIGITGAAPNANISGQRR1475 pKa = 11.84 LLGAGTARR1483 pKa = 11.84 NEE1485 pKa = 3.67 ASALTRR1491 pKa = 11.84 TMNAVDD1497 pKa = 4.06 IYY1499 pKa = 10.83 SNSWGPNDD1507 pKa = 4.21 NGRR1510 pKa = 11.84 LQAAPARR1517 pKa = 11.84 VLAALKK1523 pKa = 10.84 DD1524 pKa = 3.79 GVTNGRR1530 pKa = 11.84 DD1531 pKa = 3.45 GKK1533 pKa = 10.74 GAIYY1537 pKa = 8.85 TWAGGNGRR1545 pKa = 11.84 NSNDD1549 pKa = 2.73 NSNYY1553 pKa = 10.41 DD1554 pKa = 3.06 GYY1556 pKa = 11.73 ANSRR1560 pKa = 11.84 YY1561 pKa = 9.11 VISVAAMTNRR1571 pKa = 11.84 GRR1573 pKa = 11.84 YY1574 pKa = 8.86 SGYY1577 pKa = 9.99 SEE1579 pKa = 4.74 PGANVLVSAPSNGGTDD1595 pKa = 4.48 AITTTSTNNSYY1606 pKa = 10.75 IDD1608 pKa = 3.65 NFGGTSSATPLVSGVIALMLEE1629 pKa = 4.42 ANPNLTWRR1637 pKa = 11.84 DD1638 pKa = 3.7 VQHH1641 pKa = 6.33 VLVNSSDD1648 pKa = 3.92 VVDD1651 pKa = 4.38 ASSNGWFTNGAEE1663 pKa = 4.44 HH1664 pKa = 7.59 DD1665 pKa = 4.13 FSHH1668 pKa = 7.85 DD1669 pKa = 3.57 YY1670 pKa = 11.01 GFGRR1674 pKa = 11.84 INAEE1678 pKa = 3.74 AAVALAKK1685 pKa = 9.29 TWNNVGDD1692 pKa = 4.07 EE1693 pKa = 4.29 VSYY1696 pKa = 10.94 SASTTPGIAIPDD1708 pKa = 3.67 AGGGSISSTITISQDD1723 pKa = 2.37 ITLEE1727 pKa = 4.22 SVVIPILSDD1736 pKa = 2.87 HH1737 pKa = 6.8 TYY1739 pKa = 11.21 AGDD1742 pKa = 3.55 LTITLTSPEE1751 pKa = 4.2 GTTAILSEE1759 pKa = 4.26 GNRR1762 pKa = 11.84 RR1763 pKa = 11.84 DD1764 pKa = 3.44 TSTLNFNFSAKK1775 pKa = 8.91 TFWGEE1780 pKa = 3.65 SSRR1783 pKa = 11.84 GVWTLTINDD1792 pKa = 4.65 LASLDD1797 pKa = 4.14 TGTLDD1802 pKa = 3.18 QWGLNLYY1809 pKa = 7.55 GTQGTNFNAAITDD1822 pKa = 4.24 SITGNEE1828 pKa = 4.26 LEE1830 pKa = 4.65 TFTGASGMTYY1840 pKa = 10.55 ASKK1843 pKa = 10.56 EE1844 pKa = 3.81 ALEE1847 pKa = 4.19 QIEE1850 pKa = 4.58 GLSSYY1855 pKa = 10.46 LSHH1858 pKa = 8.53 IEE1860 pKa = 4.09 DD1861 pKa = 4.66 LLAEE1865 pKa = 4.46 SPEE1868 pKa = 4.24 KK1869 pKa = 10.44 ATGDD1873 pKa = 3.66 WLIGTQGPQDD1883 pKa = 3.51 QIMRR1887 pKa = 11.84 ASEE1890 pKa = 4.18 LGISDD1895 pKa = 4.06 SMTSQTFNAQNGISSFSMISALDD1918 pKa = 3.45 PTEE1921 pKa = 5.83 FIAQSLDD1928 pKa = 3.46 SLGTNLSYY1936 pKa = 10.72 AYY1938 pKa = 9.6 PEE1940 pKa = 4.57 LLHH1943 pKa = 5.99 EE1944 pKa = 4.38 TTTRR1948 pKa = 11.84 SLIPSLEE1955 pKa = 3.97 TSTFKK1960 pKa = 11.1 AA1961 pKa = 3.7
Molecular weight: 208.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.754
IPC2_protein 3.821
IPC_protein 3.872
Toseland 3.643
ProMoST 4.012
Dawson 3.859
Bjellqvist 3.999
Wikipedia 3.77
Rodwell 3.694
Grimsley 3.541
Solomon 3.846
Lehninger 3.808
Nozaki 3.961
DTASelect 4.215
Thurlkill 3.681
EMBOSS 3.783
Sillero 3.986
Patrickios 2.079
IPC_peptide 3.846
IPC2_peptide 3.961
IPC2.peptide.svr19 3.879
Protein with the highest isoelectric point:
>tr|A4CRW8|A4CRW8_SYNPV Uncharacterized protein OS=Synechococcus sp. (strain WH7805) OX=59931 GN=WH7805_13833 PE=4 SV=1
MM1 pKa = 7.55 TKK3 pKa = 9.01 RR4 pKa = 11.84 TLGGTSRR11 pKa = 11.84 KK12 pKa = 9.07 RR13 pKa = 11.84 KK14 pKa = 8.17 RR15 pKa = 11.84 VSGFRR20 pKa = 11.84 VRR22 pKa = 11.84 MRR24 pKa = 11.84 SHH26 pKa = 6.21 TGRR29 pKa = 11.84 RR30 pKa = 11.84 VIRR33 pKa = 11.84 TRR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.66 RR38 pKa = 11.84 GRR40 pKa = 11.84 SRR42 pKa = 11.84 LSVV45 pKa = 3.08
Molecular weight: 5.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.389
IPC_protein 12.983
Toseland 13.144
ProMoST 13.642
Dawson 13.144
Bjellqvist 13.144
Wikipedia 13.627
Rodwell 12.749
Grimsley 13.188
Solomon 13.642
Lehninger 13.554
Nozaki 13.144
DTASelect 13.144
Thurlkill 13.144
EMBOSS 13.642
Sillero 13.144
Patrickios 12.486
IPC_peptide 13.656
IPC2_peptide 12.632
IPC2.peptide.svr19 9.284
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2874
0
2874
787820
20
8129
274.1
29.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.107 ± 0.064
1.226 ± 0.027
5.66 ± 0.074
5.659 ± 0.063
3.2 ± 0.042
8.267 ± 0.06
2.07 ± 0.045
4.456 ± 0.05
2.73 ± 0.044
12.315 ± 0.098
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.066 ± 0.033
2.945 ± 0.086
5.367 ± 0.063
4.614 ± 0.056
7.016 ± 0.098
6.65 ± 0.067
5.133 ± 0.147
6.998 ± 0.046
1.721 ± 0.035
1.801 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here