Amino acid dipepetide frequency for Synechococcus sp. (strain WH7805)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	11.816AlaAla: 11.816 ± 0.22	1.437AlaCys: 1.437 ± 0.052	5.94AlaAsp: 5.94 ± 0.131	5.991AlaGlu: 5.991 ± 0.101	3.365AlaPhe: 3.365 ± 0.084	8.248AlaGly: 8.248 ± 0.123	1.662AlaHis: 1.662 ± 0.05	4.924AlaIle: 4.924 ± 0.091	2.499AlaLys: 2.499 ± 0.072	13.187AlaLeu: 13.187 ± 0.213	2.773AlaMet: 2.773 ± 0.077	2.564AlaAsn: 2.564 ± 0.082	4.515AlaPro: 4.515 ± 0.091	3.643AlaGln: 3.643 ± 0.073	5.928AlaArg: 5.928 ± 0.118	6.778AlaSer: 6.778 ± 0.136	4.562AlaThr: 4.562 ± 0.115	7.751AlaVal: 7.751 ± 0.111	1.575AlaTrp: 1.575 ± 0.052	1.908AlaTyr: 1.908 ± 0.062	0.0AlaXaa: 0.0 ± 0.0
Cys	0.976CysAla: 0.976 ± 0.039	0.259CysCys: 0.259 ± 0.021	0.731CysAsp: 0.731 ± 0.031	0.614CysGlu: 0.614 ± 0.029	0.487CysPhe: 0.487 ± 0.027	1.198CysGly: 1.198 ± 0.04	0.33CysHis: 0.33 ± 0.022	0.473CysIle: 0.473 ± 0.023	0.284CysLys: 0.284 ± 0.02	1.494CysLeu: 1.494 ± 0.056	0.212CysMet: 0.212 ± 0.016	0.334CysAsn: 0.334 ± 0.022	0.701CysPro: 0.701 ± 0.027	0.433CysGln: 0.433 ± 0.024	1.062CysArg: 1.062 ± 0.04	0.942CysSer: 0.942 ± 0.038	0.503CysThr: 0.503 ± 0.026	0.696CysVal: 0.696 ± 0.028	0.321CysTrp: 0.321 ± 0.021	0.212CysTyr: 0.212 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	5.825AspAla: 5.825 ± 0.143	0.665AspCys: 0.665 ± 0.034	3.06AspAsp: 3.06 ± 0.074	2.907AspGlu: 2.907 ± 0.083	1.747AspPhe: 1.747 ± 0.05	5.207AspGly: 5.207 ± 0.163	1.361AspHis: 1.361 ± 0.045	1.839AspIle: 1.839 ± 0.088	1.194AspLys: 1.194 ± 0.054	7.841AspLeu: 7.841 ± 0.143	0.814AspMet: 0.814 ± 0.038	1.46AspAsn: 1.46 ± 0.104	3.918AspPro: 3.918 ± 0.129	3.246AspGln: 3.246 ± 0.064	4.138AspArg: 4.138 ± 0.075	3.197AspSer: 3.197 ± 0.138	2.369AspThr: 2.369 ± 0.122	3.671AspVal: 3.671 ± 0.093	1.022AspTrp: 1.022 ± 0.035	1.125AspTyr: 1.125 ± 0.043	0.0AspXaa: 0.0 ± 0.0
Glu	6.964GluAla: 6.964 ± 0.108	0.485GluCys: 0.485 ± 0.028	2.588GluAsp: 2.588 ± 0.062	3.317GluGlu: 3.317 ± 0.094	1.325GluPhe: 1.325 ± 0.046	4.469GluGly: 4.469 ± 0.08	1.206GluHis: 1.206 ± 0.039	2.452GluIle: 2.452 ± 0.065	1.622GluLys: 1.622 ± 0.061	7.089GluLeu: 7.089 ± 0.123	1.046GluMet: 1.046 ± 0.042	1.325GluAsn: 1.325 ± 0.047	2.944GluPro: 2.944 ± 0.067	3.341GluGln: 3.341 ± 0.076	5.117GluArg: 5.117 ± 0.11	3.312GluSer: 3.312 ± 0.079	2.839GluThr: 2.839 ± 0.065	3.733GluVal: 3.733 ± 0.074	0.725GluTrp: 0.725 ± 0.03	0.696GluTyr: 0.696 ± 0.033	0.0GluXaa: 0.0 ± 0.0
Phe	2.971PheAla: 2.971 ± 0.079	0.519PheCys: 0.519 ± 0.027	2.16PheAsp: 2.16 ± 0.069	1.796PheGlu: 1.796 ± 0.049	1.161PhePhe: 1.161 ± 0.049	2.912PheGly: 2.912 ± 0.079	0.674PheHis: 0.674 ± 0.035	1.434PheIle: 1.434 ± 0.064	0.9PheLys: 0.9 ± 0.04	3.18PheLeu: 3.18 ± 0.092	0.613PheMet: 0.613 ± 0.028	1.274PheAsn: 1.274 ± 0.05	1.399PhePro: 1.399 ± 0.052	1.356PheGln: 1.356 ± 0.039	2.039PheArg: 2.039 ± 0.053	2.569PheSer: 2.569 ± 0.068	1.846PheThr: 1.846 ± 0.054	1.905PheVal: 1.905 ± 0.055	0.562PheTrp: 0.562 ± 0.029	0.729PheTyr: 0.729 ± 0.031	0.0PheXaa: 0.0 ± 0.0
Gly	7.145GlyAla: 7.145 ± 0.111	1.273GlyCys: 1.273 ± 0.04	5.004GlyAsp: 5.004 ± 0.175	4.356GlyGlu: 4.356 ± 0.087	3.161GlyPhe: 3.161 ± 0.085	6.971GlyGly: 6.971 ± 0.146	1.75GlyHis: 1.75 ± 0.052	4.104GlyIle: 4.104 ± 0.143	2.381GlyLys: 2.381 ± 0.061	10.302GlyLeu: 10.302 ± 0.145	1.863GlyMet: 1.863 ± 0.064	2.465GlyAsn: 2.465 ± 0.147	3.236GlyPro: 3.236 ± 0.087	3.441GlyGln: 3.441 ± 0.08	5.547GlyArg: 5.547 ± 0.129	5.751GlySer: 5.751 ± 0.147	4.374GlyThr: 4.374 ± 0.153	5.926GlyVal: 5.926 ± 0.087	1.814GlyTrp: 1.814 ± 0.063	1.808GlyTyr: 1.808 ± 0.053	0.0GlyXaa: 0.0 ± 0.0
His	1.738HisAla: 1.738 ± 0.059	0.4HisCys: 0.4 ± 0.023	1.031HisAsp: 1.031 ± 0.045	0.886HisGlu: 0.886 ± 0.032	0.679HisPhe: 0.679 ± 0.034	1.907HisGly: 1.907 ± 0.061	0.842HisHis: 0.842 ± 0.04	0.644HisIle: 0.644 ± 0.031	0.404HisLys: 0.404 ± 0.027	2.568HisLeu: 2.568 ± 0.068	0.27HisMet: 0.27 ± 0.021	0.562HisAsn: 0.562 ± 0.029	1.646HisPro: 1.646 ± 0.063	1.17HisGln: 1.17 ± 0.038	1.788HisArg: 1.788 ± 0.053	1.168HisSer: 1.168 ± 0.039	0.8HisThr: 0.8 ± 0.037	1.087HisVal: 1.087 ± 0.041	0.631HisTrp: 0.631 ± 0.032	0.476HisTyr: 0.476 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	4.79IleAla: 4.79 ± 0.08	0.571IleCys: 0.571 ± 0.027	3.208IleAsp: 3.208 ± 0.189	2.872IleGlu: 2.872 ± 0.068	1.136IlePhe: 1.136 ± 0.047	4.284IleGly: 4.284 ± 0.101	1.021IleHis: 1.021 ± 0.044	1.371IleIle: 1.371 ± 0.053	1.405IleLys: 1.405 ± 0.053	3.71IleLeu: 3.71 ± 0.088	0.434IleMet: 0.434 ± 0.026	1.805IleAsn: 1.805 ± 0.073	2.595IlePro: 2.595 ± 0.071	1.893IleGln: 1.893 ± 0.05	2.965IleArg: 2.965 ± 0.072	3.171IleSer: 3.171 ± 0.116	3.097IleThr: 3.097 ± 0.146	2.715IleVal: 2.715 ± 0.072	0.661IleTrp: 0.661 ± 0.028	0.852IleTyr: 0.852 ± 0.033	0.0IleXaa: 0.0 ± 0.0
Lys	3.322LysAla: 3.322 ± 0.074	0.188LysCys: 0.188 ± 0.016	1.57LysAsp: 1.57 ± 0.05	1.734LysGlu: 1.734 ± 0.059	0.613LysPhe: 0.613 ± 0.034	2.297LysGly: 2.297 ± 0.065	0.484LysHis: 0.484 ± 0.028	1.116LysIle: 1.116 ± 0.049	0.994LysLys: 0.994 ± 0.048	2.795LysLeu: 2.795 ± 0.082	0.405LysMet: 0.405 ± 0.021	0.782LysAsn: 0.782 ± 0.044	1.733LysPro: 1.733 ± 0.054	1.386LysGln: 1.386 ± 0.049	2.144LysArg: 2.144 ± 0.064	1.554LysSer: 1.554 ± 0.051	1.598LysThr: 1.598 ± 0.055	1.94LysVal: 1.94 ± 0.061	0.203LysTrp: 0.203 ± 0.016	0.439LysTyr: 0.439 ± 0.028	0.0LysXaa: 0.0 ± 0.0
Leu	12.384LeuAla: 12.384 ± 0.177	1.307LeuCys: 1.307 ± 0.049	6.783LeuAsp: 6.783 ± 0.103	7.908LeuGlu: 7.908 ± 0.161	3.511LeuPhe: 3.511 ± 0.088	9.097LeuGly: 9.097 ± 0.149	2.442LeuHis: 2.442 ± 0.069	6.022LeuIle: 6.022 ± 0.124	3.894LeuLys: 3.894 ± 0.088	15.03LeuLeu: 15.03 ± 0.277	2.883LeuMet: 2.883 ± 0.078	3.959LeuAsn: 3.959 ± 0.242	6.749LeuPro: 6.749 ± 0.127	6.246LeuGln: 6.246 ± 0.128	8.408LeuArg: 8.408 ± 0.162	8.188LeuSer: 8.188 ± 0.123	6.25LeuThr: 6.25 ± 0.249	8.637LeuVal: 8.637 ± 0.153	1.703LeuTrp: 1.703 ± 0.059	1.75LeuTyr: 1.75 ± 0.055	0.0LeuXaa: 0.0 ± 0.0
Met	2.914MetAla: 2.914 ± 0.077	0.119MetCys: 0.119 ± 0.012	1.066MetAsp: 1.066 ± 0.041	1.217MetGlu: 1.217 ± 0.042	0.466MetPhe: 0.466 ± 0.028	1.705MetGly: 1.705 ± 0.057	0.426MetHis: 0.426 ± 0.025	0.858MetIle: 0.858 ± 0.035	0.618MetLys: 0.618 ± 0.027	2.178MetLeu: 2.178 ± 0.066	0.312MetMet: 0.312 ± 0.021	0.612MetAsn: 0.612 ± 0.032	1.301MetPro: 1.301 ± 0.044	0.881MetGln: 0.881 ± 0.033	1.381MetArg: 1.381 ± 0.045	1.295MetSer: 1.295 ± 0.048	1.333MetThr: 1.333 ± 0.044	1.646MetVal: 1.646 ± 0.053	0.128MetTrp: 0.128 ± 0.013	0.199MetTyr: 0.199 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	2.935AsnAla: 2.935 ± 0.157	0.312AsnCys: 0.312 ± 0.021	1.8AsnAsp: 1.8 ± 0.09	1.266AsnGlu: 1.266 ± 0.036	0.979AsnPhe: 0.979 ± 0.051	2.857AsnGly: 2.857 ± 0.152	0.611AsnHis: 0.611 ± 0.03	1.015AsnIle: 1.015 ± 0.04	0.793AsnLys: 0.793 ± 0.038	3.26AsnLeu: 3.26 ± 0.092	0.374AsnMet: 0.374 ± 0.025	1.13AsnAsn: 1.13 ± 0.092	2.122AsnPro: 2.122 ± 0.066	1.371AsnGln: 1.371 ± 0.048	2.172AsnArg: 2.172 ± 0.058	1.862AsnSer: 1.862 ± 0.076	1.848AsnThr: 1.848 ± 0.292	1.656AsnVal: 1.656 ± 0.059	0.517AsnTrp: 0.517 ± 0.027	0.566AsnTyr: 0.566 ± 0.032	0.0AsnXaa: 0.0 ± 0.0
Pro	5.237ProAla: 5.237 ± 0.115	0.604ProCys: 0.604 ± 0.03	3.458ProAsp: 3.458 ± 0.08	3.934ProGlu: 3.934 ± 0.085	1.88ProPhe: 1.88 ± 0.062	4.462ProGly: 4.462 ± 0.09	0.952ProHis: 0.952 ± 0.039	2.267ProIle: 2.267 ± 0.063	1.387ProLys: 1.387 ± 0.049	6.611ProLeu: 6.611 ± 0.135	1.281ProMet: 1.281 ± 0.044	1.285ProAsn: 1.285 ± 0.042	2.63ProPro: 2.63 ± 0.081	2.27ProGln: 2.27 ± 0.066	2.766ProArg: 2.766 ± 0.073	3.84ProSer: 3.84 ± 0.08	2.626ProThr: 2.626 ± 0.065	4.099ProVal: 4.099 ± 0.082	1.065ProTrp: 1.065 ± 0.047	1.014ProTyr: 1.014 ± 0.042	0.0ProXaa: 0.0 ± 0.0
Gln	5.155GlnAla: 5.155 ± 0.102	0.419GlnCys: 0.419 ± 0.022	2.078GlnAsp: 2.078 ± 0.077	2.498GlnGlu: 2.498 ± 0.063	1.182GlnPhe: 1.182 ± 0.046	3.451GlnGly: 3.451 ± 0.08	0.996GlnHis: 0.996 ± 0.039	1.823GlnIle: 1.823 ± 0.05	1.201GlnLys: 1.201 ± 0.044	6.159GlnLeu: 6.159 ± 0.111	0.826GlnMet: 0.826 ± 0.036	1.028GlnAsn: 1.028 ± 0.048	2.626GlnPro: 2.626 ± 0.061	2.919GlnGln: 2.919 ± 0.085	4.323GlnArg: 4.323 ± 0.094	2.729GlnSer: 2.729 ± 0.068	2.077GlnThr: 2.077 ± 0.084	3.244GlnVal: 3.244 ± 0.084	0.864GlnTrp: 0.864 ± 0.037	0.542GlnTyr: 0.542 ± 0.028	0.0GlnXaa: 0.0 ± 0.0
Arg	5.193ArgAla: 5.193 ± 0.109	1.12ArgCys: 1.12 ± 0.039	3.653ArgAsp: 3.653 ± 0.081	3.658ArgGlu: 3.658 ± 0.082	2.879ArgPhe: 2.879 ± 0.075	4.669ArgGly: 4.669 ± 0.089	1.768ArgHis: 1.768 ± 0.053	3.239ArgIle: 3.239 ± 0.077	1.806ArgLys: 1.806 ± 0.062	9.378ArgLeu: 9.378 ± 0.175	1.577ArgMet: 1.577 ± 0.047	2.035ArgAsn: 2.035 ± 0.065	3.5ArgPro: 3.5 ± 0.082	3.841ArgGln: 3.841 ± 0.094	6.173ArgArg: 6.173 ± 0.129	4.987ArgSer: 4.987 ± 0.089	2.988ArgThr: 2.988 ± 0.074	4.335ArgVal: 4.335 ± 0.095	1.79ArgTrp: 1.79 ± 0.064	1.578ArgTyr: 1.578 ± 0.051	0.0ArgXaa: 0.0 ± 0.0
Ser	6.151SerAla: 6.151 ± 0.085	0.835SerCys: 0.835 ± 0.033	3.893SerAsp: 3.893 ± 0.122	3.571SerGlu: 3.571 ± 0.071	2.23SerPhe: 2.23 ± 0.061	6.227SerGly: 6.227 ± 0.19	1.292SerHis: 1.292 ± 0.042	3.024SerIle: 3.024 ± 0.129	1.783SerLys: 1.783 ± 0.054	8.079SerLeu: 8.079 ± 0.132	1.54SerMet: 1.54 ± 0.042	2.074SerAsn: 2.074 ± 0.113	3.265SerPro: 3.265 ± 0.073	2.575SerGln: 2.575 ± 0.061	4.422SerArg: 4.422 ± 0.095	4.882SerSer: 4.882 ± 0.101	3.675SerThr: 3.675 ± 0.083	4.514SerVal: 4.514 ± 0.107	1.205SerTrp: 1.205 ± 0.043	1.26SerTyr: 1.26 ± 0.044	0.0SerXaa: 0.0 ± 0.0
Thr	5.577ThrAla: 5.577 ± 0.249	0.49ThrCys: 0.49 ± 0.027	2.709ThrAsp: 2.709 ± 0.122	2.353ThrGlu: 2.353 ± 0.067	1.692ThrPhe: 1.692 ± 0.06	4.619ThrGly: 4.619 ± 0.221	0.869ThrHis: 0.869 ± 0.034	2.516ThrIle: 2.516 ± 0.108	1.186ThrLys: 1.186 ± 0.042	6.66ThrLeu: 6.66 ± 0.287	0.904ThrMet: 0.904 ± 0.036	1.53ThrAsn: 1.53 ± 0.112	3.46ThrPro: 3.46 ± 0.077	1.655ThrGln: 1.655 ± 0.07	2.803ThrArg: 2.803 ± 0.066	3.364ThrSer: 3.364 ± 0.159	3.13ThrThr: 3.13 ± 0.198	3.948ThrVal: 3.948 ± 0.118	0.73ThrTrp: 0.73 ± 0.037	1.131ThrTyr: 1.131 ± 0.065	0.0ThrXaa: 0.0 ± 0.0
Val	7.023ValAla: 7.023 ± 0.118	0.739ValCys: 0.739 ± 0.033	4.181ValAsp: 4.181 ± 0.112	4.069ValGlu: 4.069 ± 0.09	2.289ValPhe: 2.289 ± 0.06	5.082ValGly: 5.082 ± 0.103	1.305ValHis: 1.305 ± 0.046	3.868ValIle: 3.868 ± 0.085	1.974ValLys: 1.974 ± 0.055	9.026ValLeu: 9.026 ± 0.14	1.74ValMet: 1.74 ± 0.054	2.138ValAsn: 2.138 ± 0.054	3.381ValPro: 3.381 ± 0.073	2.77ValGln: 2.77 ± 0.061	4.128ValArg: 4.128 ± 0.095	4.351ValSer: 4.351 ± 0.089	3.685ValThr: 3.685 ± 0.165	6.232ValVal: 6.232 ± 0.125	0.933ValTrp: 0.933 ± 0.037	1.066ValTyr: 1.066 ± 0.035	0.0ValXaa: 0.0 ± 0.0
Trp	1.29TrpAla: 1.29 ± 0.045	0.267TrpCys: 0.267 ± 0.017	0.724TrpAsp: 0.724 ± 0.035	0.702TrpGlu: 0.702 ± 0.034	0.605TrpPhe: 0.605 ± 0.03	1.192TrpGly: 1.192 ± 0.047	0.371TrpHis: 0.371 ± 0.024	0.947TrpIle: 0.947 ± 0.038	0.462TrpLys: 0.462 ± 0.025	2.577TrpLeu: 2.577 ± 0.071	0.552TrpMet: 0.552 ± 0.028	0.529TrpAsn: 0.529 ± 0.03	0.952TrpPro: 0.952 ± 0.041	0.897TrpGln: 0.897 ± 0.036	1.345TrpArg: 1.345 ± 0.05	1.266TrpSer: 1.266 ± 0.045	0.835TrpThr: 0.835 ± 0.044	1.048TrpVal: 1.048 ± 0.04	0.392TrpTrp: 0.392 ± 0.025	0.256TrpTyr: 0.256 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.662TyrAla: 1.662 ± 0.047	0.254TyrCys: 0.254 ± 0.018	0.967TyrAsp: 0.967 ± 0.049	0.944TyrGlu: 0.944 ± 0.034	0.613TyrPhe: 0.613 ± 0.029	1.818TyrGly: 1.818 ± 0.05	0.334TyrHis: 0.334 ± 0.023	0.621TyrIle: 0.621 ± 0.029	0.506TyrLys: 0.506 ± 0.03	2.03TyrLeu: 2.03 ± 0.061	0.236TyrMet: 0.236 ± 0.018	0.556TyrAsn: 0.556 ± 0.031	0.994TyrPro: 0.994 ± 0.037	0.807TyrGln: 0.807 ± 0.039	1.516TyrArg: 1.516 ± 0.052	1.292TyrSer: 1.292 ± 0.045	0.891TyrThr: 0.891 ± 0.061	1.198TyrVal: 1.198 ± 0.042	0.368TyrTrp: 0.368 ± 0.021	0.406TyrTyr: 0.406 ± 0.024	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2874 proteins (787820 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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