Mycolicibacter sinensis (strain JDM601) (Mycobacterium sinense)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Mycobacteriaceae; Mycolicibacter

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4332 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F5YVZ4|F5YVZ4_MYCSD MarR family transcriptional regulator OS=Mycolicibacter sinensis (strain JDM601) OX=875328 GN=JDM601_1241 PE=4 SV=1
MM1 pKa = 6.5TTIKK5 pKa = 10.57KK6 pKa = 8.23FLCGATVAGMVAGTGAAVGVPSGLSALTADD36 pKa = 3.29WLLAAEE42 pKa = 4.4HH43 pKa = 6.29VLLIGTDD50 pKa = 3.59GTNLSKK56 pKa = 10.53ILEE59 pKa = 4.17YY60 pKa = 10.56AYY62 pKa = 10.7GDD64 pKa = 3.47DD65 pKa = 4.72SGFKK69 pKa = 9.44TAMDD73 pKa = 3.52QGITGATSEE82 pKa = 4.49VNHH85 pKa = 5.46TTISGPSWSTILTGVWDD102 pKa = 4.53DD103 pKa = 3.32KK104 pKa = 11.56HH105 pKa = 7.68GVVNNLFRR113 pKa = 11.84PEE115 pKa = 5.49PYY117 pKa = 10.21DD118 pKa = 3.07QWPTVFNLLEE128 pKa = 3.99YY129 pKa = 10.26HH130 pKa = 6.39NPDD133 pKa = 3.08IEE135 pKa = 4.45TSVIADD141 pKa = 3.24WDD143 pKa = 4.2YY144 pKa = 11.77INDD147 pKa = 3.29IGAAGGYY154 pKa = 9.71GADD157 pKa = 3.63NNLFIPFQDD166 pKa = 2.99SWADD170 pKa = 2.94TDD172 pKa = 4.13AAVTAATISQILATTTNPDD191 pKa = 2.41IDD193 pKa = 4.0TFLFSYY199 pKa = 9.78QVQVDD204 pKa = 3.97EE205 pKa = 5.33AGHH208 pKa = 5.71SFGGGSQEE216 pKa = 3.92YY217 pKa = 9.29AQAVMNVGANIQQILAAVEE236 pKa = 3.89QARR239 pKa = 11.84QEE241 pKa = 4.34TGDD244 pKa = 3.31NWSIIITTDD253 pKa = 2.75HH254 pKa = 6.28GHH256 pKa = 4.9QQSLGFGHH264 pKa = 7.34GFQSPNEE271 pKa = 3.97TSQFVIFDD279 pKa = 3.88QAGHH283 pKa = 7.24DD284 pKa = 3.87ATDD287 pKa = 4.02GSQNLNYY294 pKa = 10.09SIADD298 pKa = 3.31ITPTILQLLGVPLRR312 pKa = 11.84SDD314 pKa = 3.79FDD316 pKa = 4.14GSPMSTDD323 pKa = 3.46PAILDD328 pKa = 4.16SIVTPTDD335 pKa = 3.64LKK337 pKa = 11.09QSLSDD342 pKa = 4.46AIAMYY347 pKa = 10.04GYY349 pKa = 9.73PNIGNDD355 pKa = 2.55IMLGIRR361 pKa = 11.84TVVTSIPYY369 pKa = 10.07GINLAVTEE377 pKa = 4.26IDD379 pKa = 4.45KK380 pKa = 10.68FLQSIVDD387 pKa = 3.35QDD389 pKa = 3.21IFLISGLAEE398 pKa = 4.36LLQQVNNFVGGAVVDD413 pKa = 4.14VTDD416 pKa = 3.57TLARR420 pKa = 11.84GVAYY424 pKa = 8.73LTGSGVIAPTDD435 pKa = 3.8PPLPPPPGGSEE446 pKa = 3.79ASLFDD451 pKa = 3.99TLLSDD456 pKa = 5.09HH457 pKa = 6.68SWLSPDD463 pKa = 3.87EE464 pKa = 4.43GSFDD468 pKa = 4.34LASLLDD474 pKa = 4.81FSALLDD480 pKa = 3.63

Molecular weight: 50.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F5YWB2|F5YWB2_MYCSD Uncharacterized protein OS=Mycolicibacter sinensis (strain JDM601) OX=875328 GN=JDM601_0013 PE=3 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVTGRR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 9.68GRR42 pKa = 11.84RR43 pKa = 11.84SLTAA47 pKa = 3.9

Molecular weight: 5.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4332

0

4332

1398531

37

5188

322.8

34.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.601 ± 0.051

0.794 ± 0.01

6.302 ± 0.035

5.145 ± 0.033

2.989 ± 0.027

9.656 ± 0.158

2.192 ± 0.018

4.103 ± 0.027

2.017 ± 0.025

9.787 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.037 ± 0.019

2.229 ± 0.024

5.822 ± 0.043

3.043 ± 0.018

7.165 ± 0.048

5.175 ± 0.026

5.952 ± 0.037

8.389 ± 0.038

1.479 ± 0.016

2.123 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski