Roseovarius aestuarii

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Roseovarius

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4594 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X7BX86|A0A1X7BX86_9RHOB Ferric uptake regulation protein OS=Roseovarius aestuarii OX=475083 GN=zur_2 PE=3 SV=1
MM1 pKa = 7.89RR2 pKa = 11.84ISHH5 pKa = 7.57FYY7 pKa = 10.06TDD9 pKa = 3.56ADD11 pKa = 3.77EE12 pKa = 5.84KK13 pKa = 10.4IFGYY17 pKa = 10.09ISYY20 pKa = 10.05GWTFQNGQWVLGLTFRR36 pKa = 11.84DD37 pKa = 4.08DD38 pKa = 4.18EE39 pKa = 4.25IHH41 pKa = 7.3AGGGDD46 pKa = 3.74DD47 pKa = 4.31TIKK50 pKa = 10.99SSGGDD55 pKa = 3.44DD56 pKa = 3.08IVYY59 pKa = 9.26TGAGADD65 pKa = 3.67FVDD68 pKa = 4.16AGEE71 pKa = 4.72GNDD74 pKa = 4.45TIYY77 pKa = 11.45GDD79 pKa = 3.75AGEE82 pKa = 4.05DD83 pKa = 3.48TIYY86 pKa = 11.19GGDD89 pKa = 3.82GNDD92 pKa = 4.73FIYY95 pKa = 10.51GSPQGGLFYY104 pKa = 11.05GGDD107 pKa = 3.79GNDD110 pKa = 4.03FISGEE115 pKa = 4.24GAAHH119 pKa = 6.0VSIFGDD125 pKa = 3.35AGNDD129 pKa = 3.64TLVGGSGSDD138 pKa = 3.42TVIGGIGNDD147 pKa = 3.25TLYY150 pKa = 9.49ITEE153 pKa = 4.86GQDD156 pKa = 3.16NMHH159 pKa = 6.68GGEE162 pKa = 4.26GDD164 pKa = 3.82DD165 pKa = 5.58VFILDD170 pKa = 4.64QSVADD175 pKa = 3.96LGFRR179 pKa = 11.84TSVFDD184 pKa = 3.82YY185 pKa = 10.84FDD187 pKa = 3.73GSEE190 pKa = 4.71GYY192 pKa = 7.8DD193 pKa = 3.51TLDD196 pKa = 3.04MSAASGRR203 pKa = 11.84QVLFSWYY210 pKa = 9.8VAGGFQGITGFEE222 pKa = 4.36KK223 pKa = 10.43ILTGSGDD230 pKa = 3.56DD231 pKa = 4.87EE232 pKa = 4.47IRR234 pKa = 11.84DD235 pKa = 4.09MIGVEE240 pKa = 4.42YY241 pKa = 10.09IDD243 pKa = 4.27SGSGNDD249 pKa = 3.53LVYY252 pKa = 11.01AGGIPQVLIGGGGSDD267 pKa = 3.69TLILATLFSNPHH279 pKa = 6.93GITLDD284 pKa = 3.44AAQTSGVNGLFGNSTGHH301 pKa = 6.52VIRR304 pKa = 11.84GFEE307 pKa = 4.11NYY309 pKa = 10.0VLTFRR314 pKa = 11.84EE315 pKa = 3.95DD316 pKa = 2.91RR317 pKa = 11.84FYY319 pKa = 11.52GSNKK323 pKa = 9.74SEE325 pKa = 3.93EE326 pKa = 4.29VKK328 pKa = 10.76LGNGNDD334 pKa = 3.32IAFGGAGNDD343 pKa = 3.7TLFGQAGDD351 pKa = 4.42DD352 pKa = 3.95FLDD355 pKa = 4.18GGTGNDD361 pKa = 3.2ILRR364 pKa = 11.84GGGGADD370 pKa = 3.43EE371 pKa = 4.32FHH373 pKa = 7.28FDD375 pKa = 3.1IDD377 pKa = 4.05RR378 pKa = 11.84PFGHH382 pKa = 6.91DD383 pKa = 3.13TVHH386 pKa = 6.49GNSAVDD392 pKa = 3.32TFTFDD397 pKa = 3.21TNGMYY402 pKa = 10.26VGQVQINDD410 pKa = 4.14DD411 pKa = 3.71GDD413 pKa = 3.89DD414 pKa = 3.59AVITFLGLAGSSVTVVGAAGKK435 pKa = 8.23LTQEE439 pKa = 5.36DD440 pKa = 3.99INIIGGFGPVMPDD453 pKa = 3.21DD454 pKa = 4.07FLFF457 pKa = 4.25

Molecular weight: 47.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X7BQG0|A0A1X7BQG0_9RHOB Flagellar biosynthetic protein FlhB OS=Roseovarius aestuarii OX=475083 GN=flhB PE=3 SV=1
MM1 pKa = 7.84RR2 pKa = 11.84GALNAWVVVRR12 pKa = 11.84GALNAWVVVRR22 pKa = 11.84GALNAWVVVRR32 pKa = 11.84GALNAWVVVRR42 pKa = 11.84GTLNAWVVVRR52 pKa = 11.84GTLNALVVVRR62 pKa = 11.84GTLNALVVMRR72 pKa = 11.84GTLNTWVVMRR82 pKa = 11.84GTLNAWVVVRR92 pKa = 11.84GTLNALVVVRR102 pKa = 11.84GTLNALVVVRR112 pKa = 11.84GALNAWVVVRR122 pKa = 11.84GTLNALVVVRR132 pKa = 11.84GALNTWVVMRR142 pKa = 11.84GALNTWVVMRR152 pKa = 11.84GTLNALVVVRR162 pKa = 11.84GTLNALVVVRR172 pKa = 11.84GTLNALVVVRR182 pKa = 11.84GALNAWVVVRR192 pKa = 11.84GTLNALVVVRR202 pKa = 11.84GTLNALVVVRR212 pKa = 11.84GALNTWVVVRR222 pKa = 11.84GALNTWVVVRR232 pKa = 11.84GANARR237 pKa = 11.84FQFDD241 pKa = 3.7LFSWQFRR248 pKa = 11.84NN249 pKa = 3.75

Molecular weight: 26.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4594

0

4594

1411738

32

1938

307.3

33.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.25 ± 0.041

1.01 ± 0.012

6.206 ± 0.03

5.96 ± 0.029

3.872 ± 0.025

8.384 ± 0.038

2.191 ± 0.016

5.52 ± 0.024

3.531 ± 0.026

9.889 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.801 ± 0.017

2.951 ± 0.018

4.866 ± 0.025

3.305 ± 0.018

6.377 ± 0.03

5.543 ± 0.023

5.48 ± 0.023

7.082 ± 0.026

1.413 ± 0.016

2.368 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski