Hubei permutotetra-like virus 11
Average proteome isoelectric point is 7.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KHV9|A0A1L3KHV9_9VIRU RdRp OS=Hubei permutotetra-like virus 11 OX=1923075 PE=4 SV=1
MM1 pKa = 7.87 DD2 pKa = 5.59 ASNPVISSDD11 pKa = 3.07 RR12 pKa = 11.84 ATIQSQLEE20 pKa = 4.24 KK21 pKa = 10.89 VRR23 pKa = 11.84 GTRR26 pKa = 11.84 RR27 pKa = 11.84 QDD29 pKa = 3.01 LGYY32 pKa = 10.39 LQGLASTTQRR42 pKa = 11.84 IQLPTNINIPLLEE55 pKa = 3.86 RR56 pKa = 11.84 TGIVEE61 pKa = 3.88 KK62 pKa = 10.87 FKK64 pKa = 10.57 TNRR67 pKa = 11.84 PEE69 pKa = 3.86 VPTTKK74 pKa = 10.51 DD75 pKa = 2.78 IPLDD79 pKa = 3.66 DD80 pKa = 5.32 KK81 pKa = 11.54 IFMVEE86 pKa = 3.97 LNEE89 pKa = 4.53 CPVSGILNSNSLPLHH104 pKa = 6.02 PSGVNCLGGKK114 pKa = 9.94 VNVQRR119 pKa = 11.84 KK120 pKa = 9.07 VGGIPTAPFGLGDD133 pKa = 3.85 IVAVIGDD140 pKa = 3.78 DD141 pKa = 3.73 VLNTKK146 pKa = 9.62 HH147 pKa = 6.52 VYY149 pKa = 9.34 TSGTWSGFVKK159 pKa = 10.61 RR160 pKa = 11.84 LNQQMGRR167 pKa = 11.84 KK168 pKa = 6.79 TVSLRR173 pKa = 11.84 KK174 pKa = 8.56 TMGNMGKK181 pKa = 9.31 EE182 pKa = 4.08 LSYY185 pKa = 10.82 DD186 pKa = 3.33 ACLKK190 pKa = 10.93 LLDD193 pKa = 4.48 KK194 pKa = 11.2 YY195 pKa = 10.35 MPRR198 pKa = 11.84 KK199 pKa = 9.31 PNYY202 pKa = 8.86 DD203 pKa = 2.74 WPGRR207 pKa = 11.84 NEE209 pKa = 3.83 ANFYY213 pKa = 11.14 EE214 pKa = 4.92 DD215 pKa = 3.77 LTTKK219 pKa = 10.36 IKK221 pKa = 10.06 VTMNSSAGAPYY232 pKa = 9.06 WRR234 pKa = 11.84 NKK236 pKa = 8.5 SEE238 pKa = 3.95 CMEE241 pKa = 5.17 DD242 pKa = 3.5 IIDD245 pKa = 3.71 VGIPLIVKK253 pKa = 9.89 AIKK256 pKa = 10.21 EE257 pKa = 3.93 NSLDD261 pKa = 3.53 KK262 pKa = 11.06 LYY264 pKa = 10.55 MEE266 pKa = 4.61 NPEE269 pKa = 4.11 MFLCEE274 pKa = 4.4 VKK276 pKa = 10.93 NKK278 pKa = 7.46 TDD280 pKa = 3.15 RR281 pKa = 11.84 YY282 pKa = 9.33 EE283 pKa = 3.91 VSKK286 pKa = 11.19 LKK288 pKa = 10.91 DD289 pKa = 2.85 KK290 pKa = 9.98 TRR292 pKa = 11.84 PYY294 pKa = 9.81 TCVPAHH300 pKa = 6.06 WAFLFSMLTQGFQEE314 pKa = 4.2 TLLTFDD320 pKa = 4.55 QDD322 pKa = 3.8 PSSSNAYY329 pKa = 9.88 GFSSSNGGLKK339 pKa = 10.48 RR340 pKa = 11.84 MVEE343 pKa = 3.77 WMYY346 pKa = 10.09 TADD349 pKa = 3.65 RR350 pKa = 11.84 RR351 pKa = 11.84 GKK353 pKa = 8.35 VVCYY357 pKa = 10.56 GDD359 pKa = 3.91 DD360 pKa = 3.3 ACIVIKK366 pKa = 10.68 KK367 pKa = 9.45 GDD369 pKa = 3.66 KK370 pKa = 9.88 IYY372 pKa = 10.82 RR373 pKa = 11.84 IDD375 pKa = 3.99 PDD377 pKa = 4.55 FKK379 pKa = 11.53 QMDD382 pKa = 3.82 GSLSQEE388 pKa = 4.41 DD389 pKa = 3.64 IEE391 pKa = 5.04 LTVKK395 pKa = 9.68 WVMAHH400 pKa = 5.89 FEE402 pKa = 4.11 KK403 pKa = 11.0 DD404 pKa = 2.91 AGKK407 pKa = 10.42 QSLFWRR413 pKa = 11.84 AVAGVWALMSHH424 pKa = 6.02 NPVFVIDD431 pKa = 4.17 GKK433 pKa = 8.28 TVYY436 pKa = 10.22 RR437 pKa = 11.84 KK438 pKa = 9.95 RR439 pKa = 11.84 NPSGLMTGVPGTTLFDD455 pKa = 3.7 TVKK458 pKa = 10.97 SVMVWNKK465 pKa = 10.32 LLDD468 pKa = 3.59 VCATGQGDD476 pKa = 3.7 ILDD479 pKa = 4.48 EE480 pKa = 4.22 KK481 pKa = 10.75 WVTNWMLQNGLVVKK495 pKa = 10.43 EE496 pKa = 4.43 GTWKK500 pKa = 10.47 PEE502 pKa = 3.96 EE503 pKa = 4.24 LPKK506 pKa = 10.78 QLDD509 pKa = 3.37 HH510 pKa = 6.98 GDD512 pKa = 3.42 LVTTHH517 pKa = 7.02 KK518 pKa = 10.74 FLGVQIKK525 pKa = 10.23 KK526 pKa = 10.23 IIWKK530 pKa = 8.68 GTEE533 pKa = 3.87 QFVPTIPYY541 pKa = 9.2 EE542 pKa = 4.31 DD543 pKa = 3.87 AVEE546 pKa = 3.94 MLVIQKK552 pKa = 10.37 DD553 pKa = 4.2 DD554 pKa = 3.43 PWPTNKK560 pKa = 9.44 VSNTMKK566 pKa = 10.62 ARR568 pKa = 11.84 TLFDD572 pKa = 3.36 RR573 pKa = 11.84 MRR575 pKa = 11.84 GLMITMGFNHH585 pKa = 7.12 PAIVDD590 pKa = 4.42 CIHH593 pKa = 6.41 NVVNNLPPEE602 pKa = 4.52 VILMQVQNGVGEE614 pKa = 4.4 KK615 pKa = 9.32 PDD617 pKa = 4.86 HH618 pKa = 6.16 ITLQEE623 pKa = 3.78 FNYY626 pKa = 9.17 PDD628 pKa = 3.39 SSGFPSVDD636 pKa = 3.73 FCISVYY642 pKa = 11.23 ADD644 pKa = 3.24 GTYY647 pKa = 10.56 DD648 pKa = 3.71 EE649 pKa = 4.9 PWIKK653 pKa = 10.6 LFPNLDD659 pKa = 3.77 DD660 pKa = 5.47 KK661 pKa = 11.46 ISAFKK666 pKa = 10.51 EE667 pKa = 3.79 QIKK670 pKa = 10.4 QMEE673 pKa = 4.2 RR674 pKa = 11.84 KK675 pKa = 9.13 YY676 pKa = 10.92 RR677 pKa = 11.84 EE678 pKa = 3.92 LLGVQDD684 pKa = 3.83 PQYY687 pKa = 11.64 VEE689 pKa = 5.61 IEE691 pKa = 4.29 DD692 pKa = 3.91 KK693 pKa = 10.87 PLNQEE698 pKa = 4.04 YY699 pKa = 10.74 QLVEE703 pKa = 3.86 AKK705 pKa = 10.11 TPVKK709 pKa = 9.9 PPKK712 pKa = 10.05 FEE714 pKa = 4.45 SVNKK718 pKa = 9.63 RR719 pKa = 11.84 SKK721 pKa = 9.52 VCEE724 pKa = 3.85 VHH726 pKa = 7.63 DD727 pKa = 5.96 DD728 pKa = 4.43 RR729 pKa = 11.84 IQDD732 pKa = 3.25 RR733 pKa = 11.84 KK734 pKa = 10.28 YY735 pKa = 11.06 VPNLGQSVRR744 pKa = 11.84 IFLQKK749 pKa = 10.72 GYY751 pKa = 10.34 GLAPVGVVAAGLGVSARR768 pKa = 11.84 RR769 pKa = 11.84 LYY771 pKa = 10.8 RR772 pKa = 11.84 EE773 pKa = 3.81 ASNWGYY779 pKa = 11.65 YY780 pKa = 5.82 MTGWTVDD787 pKa = 4.53 DD788 pKa = 3.92 ICSLRR793 pKa = 11.84 PIITAFKK800 pKa = 8.78 THH802 pKa = 6.19 QEE804 pKa = 4.27 DD805 pKa = 4.09 IFDD808 pKa = 4.21 DD809 pKa = 4.02 MEE811 pKa = 4.49 GKK813 pKa = 8.26 KK814 pKa = 8.44 TLINKK819 pKa = 7.42 STEE822 pKa = 3.41 IRR824 pKa = 11.84 QEE826 pKa = 3.95 SLQKK830 pKa = 10.13 SGEE833 pKa = 4.4 VVRR836 pKa = 11.84 SAPDD840 pKa = 3.31 LVLLRR845 pKa = 11.84 KK846 pKa = 10.24 DD847 pKa = 2.9 ILLFNQSAPVSQVDD861 pKa = 3.56 NADD864 pKa = 3.54 EE865 pKa = 4.14 AFKK868 pKa = 10.7 ILNLMVSRR876 pKa = 11.84 EE877 pKa = 3.81 GLQYY881 pKa = 11.02 TFTTKK886 pKa = 9.9 VDD888 pKa = 3.35 ATKK891 pKa = 10.28 VNSVGVVMVARR902 pKa = 11.84 HH903 pKa = 5.8 SSTGVPSGWTPVAEE917 pKa = 3.91 AWSLNKK923 pKa = 10.04 KK924 pKa = 8.68 LAKK927 pKa = 10.13 EE928 pKa = 4.52 FIARR932 pKa = 11.84 SILEE936 pKa = 4.03 MNGIEE941 pKa = 4.16 CEE943 pKa = 3.85 EE944 pKa = 4.5 SKK946 pKa = 11.03 FSVTYY951 pKa = 9.66 VPPILEE957 pKa = 4.51 EE958 pKa = 4.06 SLNWADD964 pKa = 3.65 EE965 pKa = 4.42 VEE967 pKa = 4.43 NTFNPRR973 pKa = 11.84 QIPRR977 pKa = 11.84 VIDD980 pKa = 3.67 EE981 pKa = 4.61 KK982 pKa = 11.25 NSNVLPDD989 pKa = 3.74 LDD991 pKa = 4.21 VYY993 pKa = 11.17 QEE995 pKa = 4.13 LEE997 pKa = 3.9 KK998 pKa = 10.84 LRR1000 pKa = 11.84 PYY1002 pKa = 10.17 IPKK1005 pKa = 10.16 KK1006 pKa = 9.38 FLGYY1010 pKa = 10.61 AEE1012 pKa = 4.39 SLFVKK1017 pKa = 9.95 PNPQEE1022 pKa = 3.89 HH1023 pKa = 6.45 LKK1025 pKa = 10.81 AALEE1029 pKa = 3.99 MLDD1032 pKa = 3.84 KK1033 pKa = 10.31 IDD1035 pKa = 3.72 RR1036 pKa = 11.84 QVHH1039 pKa = 5.42 HH1040 pKa = 7.03 WSSSDD1045 pKa = 3.16 SDD1047 pKa = 3.76 TSGVGSSSPSTPSKK1061 pKa = 10.46 RR1062 pKa = 11.84 SRR1064 pKa = 11.84 LTPARR1069 pKa = 11.84 RR1070 pKa = 11.84 TKK1072 pKa = 10.81 LNRR1075 pKa = 11.84 KK1076 pKa = 6.29 LTEE1079 pKa = 3.29 RR1080 pKa = 11.84 RR1081 pKa = 11.84 RR1082 pKa = 11.84 RR1083 pKa = 11.84 KK1084 pKa = 9.55 KK1085 pKa = 10.56 ASSTTKK1091 pKa = 10.12 PP1092 pKa = 3.18
Molecular weight: 123.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.118
IPC2_protein 7.629
IPC_protein 7.439
Toseland 7.22
ProMoST 8.097
Dawson 8.258
Bjellqvist 8.492
Wikipedia 8.185
Rodwell 8.302
Grimsley 7.278
Solomon 8.302
Lehninger 8.302
Nozaki 8.697
DTASelect 8.287
Thurlkill 8.375
EMBOSS 8.448
Sillero 8.653
Patrickios 4.622
IPC_peptide 8.287
IPC2_peptide 7.249
IPC2.peptide.svr19 7.693
Protein with the highest isoelectric point:
>tr|A0A1L3KHV9|A0A1L3KHV9_9VIRU RdRp OS=Hubei permutotetra-like virus 11 OX=1923075 PE=4 SV=1
MM1 pKa = 6.97 VGSVEE6 pKa = 5.12 CEE8 pKa = 3.8 YY9 pKa = 10.96 CKK11 pKa = 10.79 KK12 pKa = 10.45 KK13 pKa = 10.78 FKK15 pKa = 10.77 SKK17 pKa = 9.95 RR18 pKa = 11.84 AKK20 pKa = 8.4 NQHH23 pKa = 4.69 VSMVHH28 pKa = 6.94 KK29 pKa = 10.14 INEE32 pKa = 4.1 KK33 pKa = 8.71 MAPPAVAKK41 pKa = 9.73 TGRR44 pKa = 11.84 TRR46 pKa = 11.84 GSLRR50 pKa = 11.84 LRR52 pKa = 11.84 RR53 pKa = 11.84 TRR55 pKa = 11.84 TSNLPGFDD63 pKa = 3.31 IAPSRR68 pKa = 11.84 VPTVRR73 pKa = 11.84 GGMINISGEE82 pKa = 3.97 DD83 pKa = 3.28 RR84 pKa = 11.84 IGAFDD89 pKa = 4.46 LKK91 pKa = 10.72 SGKK94 pKa = 10.05 AVFMSVDD101 pKa = 3.57 ISPSMSARR109 pKa = 11.84 LTTIARR115 pKa = 11.84 AYY117 pKa = 10.39 QRR119 pKa = 11.84 IKK121 pKa = 9.44 WNAVKK126 pKa = 10.35 IVVTPQASAMTNGGYY141 pKa = 10.01 VCGFIADD148 pKa = 3.86 PSDD151 pKa = 3.57 RR152 pKa = 11.84 AVTASDD158 pKa = 3.99 LSASQGAQTKK168 pKa = 9.89 KK169 pKa = 10.01 FYY171 pKa = 8.59 EE172 pKa = 4.21 TAVVYY177 pKa = 8.45 MPRR180 pKa = 11.84 KK181 pKa = 8.11 TDD183 pKa = 3.81 LLYY186 pKa = 10.66 TSAGEE191 pKa = 4.05 DD192 pKa = 3.19 PRR194 pKa = 11.84 LFMPASFWIISEE206 pKa = 4.27 GLPSSNLTMIVSVVWDD222 pKa = 3.72 VTLSQPTLEE231 pKa = 4.24 NSHH234 pKa = 6.41 NNSFLLVGEE243 pKa = 4.7 IVPNPANYY251 pKa = 9.85 NLRR254 pKa = 11.84 YY255 pKa = 9.52 SPPGGDD261 pKa = 3.67 PQDD264 pKa = 4.29 DD265 pKa = 3.76 ASSIIPAVLRR275 pKa = 11.84 EE276 pKa = 4.23 TPGYY280 pKa = 10.5 HH281 pKa = 5.91 YY282 pKa = 10.72 FRR284 pKa = 11.84 VPTFTIEE291 pKa = 4.02 YY292 pKa = 10.33 SEE294 pKa = 4.46 GTGDD298 pKa = 3.17 TGTIQAHH305 pKa = 4.63 FVVYY309 pKa = 8.48 HH310 pKa = 5.01 TTDD313 pKa = 2.63 KK314 pKa = 11.14 KK315 pKa = 11.08 LYY317 pKa = 8.26 YY318 pKa = 10.02 SSNGRR323 pKa = 11.84 DD324 pKa = 3.2 VEE326 pKa = 4.4 TTMWQGNVDD335 pKa = 3.31 AHH337 pKa = 5.12 QTLVPCGTFMKK348 pKa = 10.81 YY349 pKa = 9.92 VGQEE353 pKa = 3.6 NSCRR357 pKa = 11.84 DD358 pKa = 3.8 TKK360 pKa = 11.36 LLTPKK365 pKa = 10.21 LLSEE369 pKa = 4.55 TSPGSTSSSTNLSAKK384 pKa = 6.77 MQKK387 pKa = 10.52 LEE389 pKa = 4.09 TLFLEE394 pKa = 5.76 LKK396 pKa = 10.53 QNYY399 pKa = 9.19 QKK401 pKa = 10.99 DD402 pKa = 4.19 SKK404 pKa = 11.0 PLMEE408 pKa = 5.02 EE409 pKa = 4.28 SMILKK414 pKa = 10.01 PNLVEE419 pKa = 4.87 KK420 pKa = 11.06 LEE422 pKa = 3.91 QLEE425 pKa = 4.25 LL426 pKa = 4.42
Molecular weight: 47.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.372
IPC2_protein 8.331
IPC_protein 8.244
Toseland 8.756
ProMoST 8.726
Dawson 9.136
Bjellqvist 9.048
Wikipedia 9.282
Rodwell 9.282
Grimsley 9.107
Solomon 9.224
Lehninger 9.18
Nozaki 9.136
DTASelect 8.931
Thurlkill 9.033
EMBOSS 9.268
Sillero 9.209
Patrickios 4.546
IPC_peptide 9.209
IPC2_peptide 7.717
IPC2.peptide.svr19 7.656
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1518
426
1092
759.0
85.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.007 ± 0.676
1.252 ± 0.04
5.929 ± 0.746
5.797 ± 0.321
3.36 ± 0.037
6.192 ± 0.074
1.515 ± 0.065
5.072 ± 0.311
7.773 ± 0.61
8.3 ± 0.162
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.096 ± 0.216
4.809 ± 0.177
5.863 ± 0.122
3.821 ± 0.152
5.007 ± 0.039
7.642 ± 1.126
6.653 ± 0.793
8.103 ± 0.419
1.647 ± 0.359
3.162 ± 0.301
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here