Streptococcus phage Javan349
Average proteome isoelectric point is 6.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6A8J6|A0A4D6A8J6_9CAUD Uncharacterized protein OS=Streptococcus phage Javan349 OX=2548119 GN=Javan349_0034 PE=4 SV=1
MM1 pKa = 7.61 SYY3 pKa = 11.05 DD4 pKa = 3.95 LEE6 pKa = 4.43 ILGKK10 pKa = 9.83 IEE12 pKa = 3.96 SGQYY16 pKa = 9.97 ICIDD20 pKa = 3.51 EE21 pKa = 4.67 PKK23 pKa = 10.37 YY24 pKa = 10.99 SSPTYY29 pKa = 10.74 NLGKK33 pKa = 9.1 MFRR36 pKa = 11.84 VAMDD40 pKa = 3.16 WDD42 pKa = 4.41 FDD44 pKa = 3.41 QGTIYY49 pKa = 10.78 NIADD53 pKa = 3.09 IFEE56 pKa = 4.69 NIQRR60 pKa = 11.84 GISEE64 pKa = 4.49 LEE66 pKa = 3.95 QYY68 pKa = 9.17 PEE70 pKa = 4.81 KK71 pKa = 10.64 YY72 pKa = 9.59 VQYY75 pKa = 10.66 EE76 pKa = 4.03 PANKK80 pKa = 9.13 WGTVSSALEE89 pKa = 4.03 DD90 pKa = 3.6 LRR92 pKa = 11.84 SLRR95 pKa = 11.84 DD96 pKa = 3.75 CILGQDD102 pKa = 3.23 IDD104 pKa = 3.94 TKK106 pKa = 10.54 YY107 pKa = 11.13 LYY109 pKa = 10.61 VRR111 pKa = 11.84 WW112 pKa = 3.86
Molecular weight: 13.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.057
IPC2_protein 4.38
IPC_protein 4.279
Toseland 4.101
ProMoST 4.406
Dawson 4.24
Bjellqvist 4.393
Wikipedia 4.139
Rodwell 4.113
Grimsley 4.012
Solomon 4.24
Lehninger 4.19
Nozaki 4.355
DTASelect 4.546
Thurlkill 4.126
EMBOSS 4.151
Sillero 4.393
Patrickios 1.99
IPC_peptide 4.24
IPC2_peptide 4.38
IPC2.peptide.svr19 4.306
Protein with the highest isoelectric point:
>tr|A0A4D6AC19|A0A4D6AC19_9CAUD Mobile element protein OS=Streptococcus phage Javan349 OX=2548119 GN=Javan349_0027 PE=3 SV=1
MM1 pKa = 7.62 SSGAEE6 pKa = 3.59 LRR8 pKa = 11.84 GFDD11 pKa = 3.96 DD12 pKa = 3.31 VLRR15 pKa = 11.84 NIEE18 pKa = 3.98 VRR20 pKa = 11.84 LGNNKK25 pKa = 8.78 VKK27 pKa = 10.63 RR28 pKa = 11.84 ATSRR32 pKa = 11.84 ALKK35 pKa = 10.36 AVANEE40 pKa = 3.87 TLEE43 pKa = 4.15 EE44 pKa = 4.1 FKK46 pKa = 11.18 GALQVYY52 pKa = 9.16 KK53 pKa = 10.12 DD54 pKa = 3.43 TGEE57 pKa = 4.35 TIEE60 pKa = 4.32 SATAGRR66 pKa = 11.84 VTGLASGVPVVKK78 pKa = 10.19 IGFGEE83 pKa = 4.76 GSRR86 pKa = 11.84 WRR88 pKa = 11.84 LVHH91 pKa = 6.68 LNEE94 pKa = 4.47 FGYY97 pKa = 10.93 GKK99 pKa = 9.95 NPHH102 pKa = 6.4 PRR104 pKa = 11.84 GFGVIRR110 pKa = 11.84 RR111 pKa = 11.84 FSEE114 pKa = 4.0 AHH116 pKa = 5.54 AKK118 pKa = 7.8 TYY120 pKa = 9.96 KK121 pKa = 10.43 YY122 pKa = 10.32 RR123 pKa = 11.84 VASHH127 pKa = 6.35 LKK129 pKa = 10.01 IGGFF133 pKa = 3.68
Molecular weight: 14.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.18
IPC2_protein 9.458
IPC_protein 9.633
Toseland 10.35
ProMoST 9.926
Dawson 10.467
Bjellqvist 10.101
Wikipedia 10.628
Rodwell 10.906
Grimsley 10.526
Solomon 10.511
Lehninger 10.496
Nozaki 10.306
DTASelect 10.101
Thurlkill 10.35
EMBOSS 10.73
Sillero 10.379
Patrickios 10.643
IPC_peptide 10.526
IPC2_peptide 8.595
IPC2.peptide.svr19 8.67
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
49
0
49
11633
37
1422
237.4
26.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.714 ± 0.674
0.576 ± 0.122
6.112 ± 0.313
7.462 ± 0.336
4.504 ± 0.341
6.542 ± 0.33
1.53 ± 0.143
6.696 ± 0.295
8.588 ± 0.419
8.192 ± 0.362
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.493 ± 0.201
5.751 ± 0.291
2.828 ± 0.199
3.705 ± 0.191
4.479 ± 0.315
6.585 ± 0.364
6.232 ± 0.457
6.052 ± 0.248
1.264 ± 0.151
3.696 ± 0.297
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here