Otolemur garnettii (Small-eared galago) (Garnett s greater bushbaby)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires;

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 19931 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H0XWI8|H0XWI8_OTOGA Uncharacterized protein OS=Otolemur garnettii OX=30611 PE=3 SV=1
PP1 pKa = 6.9ATSLLLLLLLLFLRR15 pKa = 11.84CSEE18 pKa = 4.18LGEE21 pKa = 4.16GVRR24 pKa = 11.84FGQKK28 pKa = 8.14DD29 pKa = 3.73TKK31 pKa = 10.2VLRR34 pKa = 11.84ARR36 pKa = 11.84RR37 pKa = 11.84DD38 pKa = 3.38GVRR41 pKa = 11.84VLGRR45 pKa = 11.84GTSWASDD52 pKa = 3.08RR53 pKa = 11.84AGPEE57 pKa = 3.33QRR59 pKa = 11.84YY60 pKa = 9.56GIVGTRR66 pKa = 11.84QNVSTEE72 pKa = 4.34GPASSHH78 pKa = 5.85EE79 pKa = 4.04NSGTRR84 pKa = 11.84EE85 pKa = 4.01NDD87 pKa = 3.03STTDD91 pKa = 3.2PGTTEE96 pKa = 5.35DD97 pKa = 4.34YY98 pKa = 9.95VTTEE102 pKa = 4.87DD103 pKa = 5.32YY104 pKa = 10.13VTTDD108 pKa = 3.16PGTTDD113 pKa = 4.69DD114 pKa = 5.38YY115 pKa = 11.44VTTDD119 pKa = 3.83LRR121 pKa = 11.84TTDD124 pKa = 4.87DD125 pKa = 4.29YY126 pKa = 11.82VTTDD130 pKa = 3.01PGTTEE135 pKa = 5.66DD136 pKa = 5.15YY137 pKa = 8.6VTPDD141 pKa = 3.34PGTTDD146 pKa = 4.5DD147 pKa = 5.59YY148 pKa = 11.47VTTDD152 pKa = 3.01PGTTEE157 pKa = 5.4DD158 pKa = 5.17YY159 pKa = 9.97VTTDD163 pKa = 3.92LGTTDD168 pKa = 5.56DD169 pKa = 5.04YY170 pKa = 11.76VTTDD174 pKa = 3.01PGTTEE179 pKa = 5.43DD180 pKa = 4.57YY181 pKa = 8.14TTEE184 pKa = 4.73DD185 pKa = 4.34YY186 pKa = 10.2VTTDD190 pKa = 2.82PGTTEE195 pKa = 5.47DD196 pKa = 5.27YY197 pKa = 9.98VTTDD201 pKa = 2.82PGTTEE206 pKa = 5.47DD207 pKa = 4.63YY208 pKa = 9.16VTSDD212 pKa = 2.69RR213 pKa = 11.84GTTEE217 pKa = 5.32DD218 pKa = 4.41YY219 pKa = 9.28VTSDD223 pKa = 2.69RR224 pKa = 11.84GTTEE228 pKa = 5.27DD229 pKa = 5.4YY230 pKa = 10.0VTTDD234 pKa = 2.42QGTTEE239 pKa = 5.13DD240 pKa = 4.65YY241 pKa = 9.99VTTDD245 pKa = 3.95LEE247 pKa = 4.69TTAHH251 pKa = 6.38

Molecular weight: 27.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H0XLT0|H0XLT0_OTOGA Uncharacterized protein OS=Otolemur garnettii OX=30611 PE=4 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.12WRR6 pKa = 11.84KK7 pKa = 9.1KK8 pKa = 9.32RR9 pKa = 11.84MRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 10.08RR15 pKa = 11.84KK16 pKa = 8.21RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 8.46MRR21 pKa = 11.84QRR23 pKa = 11.84SKK25 pKa = 11.41

Molecular weight: 3.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19446

485

19931

10768681

16

8779

540.3

60.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.853 ± 0.017

2.291 ± 0.015

4.763 ± 0.013

6.994 ± 0.023

3.781 ± 0.013

6.49 ± 0.023

2.639 ± 0.009

4.463 ± 0.014

5.808 ± 0.022

10.089 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.14 ± 0.009

3.633 ± 0.012

6.128 ± 0.027

4.739 ± 0.015

5.56 ± 0.016

8.271 ± 0.022

5.316 ± 0.015

6.078 ± 0.013

1.212 ± 0.006

2.743 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski