Vibrio sp. C7
Average proteome isoelectric point is 6.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7458 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A090R0Z4|A0A090R0Z4_9VIBR Uncharacterized protein OS=Vibrio sp. C7 OX=1001886 GN=JCM19233_80 PE=4 SV=1
MM1 pKa = 7.67 GDD3 pKa = 4.04 IDD5 pKa = 4.05 PTDD8 pKa = 4.85 PIEE11 pKa = 4.49 PPDD14 pKa = 4.11 GGFEE18 pKa = 4.01 PPPIGGDD25 pKa = 3.29 NPLPPVDD32 pKa = 4.49 INPPDD37 pKa = 4.75 EE38 pKa = 5.22 IPPQDD43 pKa = 3.78 GTDD46 pKa = 3.55 SAVLQAIQNQHH57 pKa = 6.16 RR58 pKa = 11.84 DD59 pKa = 3.36 MNSALNHH66 pKa = 6.16 ISSDD70 pKa = 3.84 LNHH73 pKa = 6.76 GFADD77 pKa = 3.56 VNNNLRR83 pKa = 11.84 SINNTNNAIGQTIVDD98 pKa = 4.14 QMNQDD103 pKa = 3.55 YY104 pKa = 10.64 EE105 pKa = 4.27 IYY107 pKa = 9.87 QAQRR111 pKa = 11.84 DD112 pKa = 3.9 LTLQNTAATYY122 pKa = 11.4 NMASTISGSIDD133 pKa = 3.35 GQTDD137 pKa = 3.18 NLLDD141 pKa = 3.89 GLSNHH146 pKa = 6.12 GQSIVGAIDD155 pKa = 3.49 GLGEE159 pKa = 4.08 SLEE162 pKa = 4.41 GVGGCTPNEE171 pKa = 4.06 EE172 pKa = 4.12 NNYY175 pKa = 7.74 TCEE178 pKa = 3.91 GRR180 pKa = 11.84 TGMDD184 pKa = 3.55 SDD186 pKa = 4.86 LVWDD190 pKa = 3.43 ITGDD194 pKa = 3.05 INTQVDD200 pKa = 3.97 SEE202 pKa = 4.44 YY203 pKa = 11.14 SSAFGSVVSGAQSYY217 pKa = 11.01 IDD219 pKa = 3.96 GGQTDD224 pKa = 3.82 EE225 pKa = 4.75 MKK227 pKa = 11.06 GVMDD231 pKa = 3.88 SAIGVLFGGLPNIGSCTPFSLPMPNGNEE259 pKa = 3.7 VSFGCEE265 pKa = 3.37 FSDD268 pKa = 4.01 KK269 pKa = 10.66 FKK271 pKa = 11.24 LIASWLIYY279 pKa = 10.08 IYY281 pKa = 8.42 TVWTLIDD288 pKa = 3.92 LMLNGINPAQRR299 pKa = 11.84 KK300 pKa = 7.44 EE301 pKa = 4.02 SS302 pKa = 3.55
Molecular weight: 32.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.713
IPC2_protein 3.63
IPC_protein 3.656
Toseland 3.427
ProMoST 3.834
Dawson 3.656
Bjellqvist 3.821
Wikipedia 3.617
Rodwell 3.478
Grimsley 3.338
Solomon 3.656
Lehninger 3.605
Nozaki 3.77
DTASelect 4.037
Thurlkill 3.49
EMBOSS 3.617
Sillero 3.783
Patrickios 0.846
IPC_peptide 3.643
IPC2_peptide 3.757
IPC2.peptide.svr19 3.722
Protein with the highest isoelectric point:
>tr|A0A090RC31|A0A090RC31_9VIBR Oxygen-insensitive NAD(P)H nitroreductase OS=Vibrio sp. C7 OX=1001886 GN=JCM19233_3038 PE=4 SV=1
MM1 pKa = 6.9 QQTGTQQRR9 pKa = 11.84 VRR11 pKa = 11.84 ALLLSGIRR19 pKa = 11.84 SAVLWRR25 pKa = 11.84 QVGGKK30 pKa = 8.99 RR31 pKa = 11.84 RR32 pKa = 11.84 HH33 pKa = 6.43 LIFSRR38 pKa = 11.84 KK39 pKa = 9.72 KK40 pKa = 9.28 MVEE43 pKa = 3.58 QAQILLARR51 pKa = 11.84 MM52 pKa = 3.83
Molecular weight: 6.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.343
IPC2_protein 10.73
IPC_protein 12.325
Toseland 12.486
ProMoST 12.983
Dawson 12.486
Bjellqvist 12.486
Wikipedia 12.954
Rodwell 12.149
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 11.901
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.124
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7458
0
7458
1439835
37
1193
193.1
21.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.388 ± 0.034
1.062 ± 0.011
5.54 ± 0.03
6.46 ± 0.028
4.124 ± 0.025
6.721 ± 0.03
2.245 ± 0.016
6.236 ± 0.026
5.077 ± 0.024
10.182 ± 0.034
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.903 ± 0.017
4.212 ± 0.022
3.785 ± 0.02
4.536 ± 0.025
4.611 ± 0.023
6.901 ± 0.025
5.495 ± 0.025
7.185 ± 0.027
1.276 ± 0.013
3.061 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here