Aeromicrobium camelliae
Average proteome isoelectric point is 5.89
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2929 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3N6X3D5|A0A3N6X3D5_9ACTN DNA polymerase III subunit delta' OS=Aeromicrobium camelliae OX=1538144 GN=EHW97_07580 PE=4 SV=1
MM1 pKa = 7.19 NRR3 pKa = 11.84 LLAALALVLSGLVVTAQPAAAHH25 pKa = 5.93 GQCEE29 pKa = 4.67 GPSDD33 pKa = 3.62 IPILVEE39 pKa = 3.83 YY40 pKa = 10.39 GSLAGAPTTVCAQGAAGMRR59 pKa = 11.84 ALDD62 pKa = 4.35 ALSMAGIEE70 pKa = 4.12 IEE72 pKa = 4.29 GTAEE76 pKa = 3.91 YY77 pKa = 10.47 GDD79 pKa = 3.98 SVVCRR84 pKa = 11.84 VNGLPDD90 pKa = 3.79 EE91 pKa = 4.38 EE92 pKa = 5.9 AEE94 pKa = 4.46 PCTGMPAADD103 pKa = 4.59 AYY105 pKa = 9.2 WAFYY109 pKa = 9.2 MATDD113 pKa = 4.66 GAPWQYY119 pKa = 10.89 AQSGVSEE126 pKa = 4.18 QVLEE130 pKa = 4.43 EE131 pKa = 4.32 GDD133 pKa = 4.23 FVALSFQEE141 pKa = 4.64 GGEE144 pKa = 4.19 SLPTVAADD152 pKa = 3.38 AQLRR156 pKa = 11.84 ADD158 pKa = 3.66 AHH160 pKa = 6.61 APASDD165 pKa = 3.91 ANAADD170 pKa = 4.21 DD171 pKa = 4.45 PEE173 pKa = 4.21 AHH175 pKa = 5.97 EE176 pKa = 4.64 TADD179 pKa = 3.61 EE180 pKa = 4.42 ADD182 pKa = 4.64 DD183 pKa = 4.41 GGADD187 pKa = 3.55 LGLVIGIIVLLAVIGAAAVILARR210 pKa = 11.84 RR211 pKa = 11.84 RR212 pKa = 11.84 PSAA215 pKa = 3.39
Molecular weight: 21.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.712
IPC2_protein 3.795
IPC_protein 3.77
Toseland 3.567
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.668
Rodwell 3.605
Grimsley 3.478
Solomon 3.745
Lehninger 3.694
Nozaki 3.872
DTASelect 4.062
Thurlkill 3.617
EMBOSS 3.681
Sillero 3.897
Patrickios 1.036
IPC_peptide 3.745
IPC2_peptide 3.872
IPC2.peptide.svr19 3.791
Protein with the highest isoelectric point:
>tr|A0A3N6Z439|A0A3N6Z439_9ACTN XRE family transcriptional regulator OS=Aeromicrobium camelliae OX=1538144 GN=EHW97_15040 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 9.97 KK16 pKa = 9.33 HH17 pKa = 4.25 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AIISSRR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 9.86 GRR40 pKa = 11.84 AKK42 pKa = 10.43 LAAA45 pKa = 4.52
Molecular weight: 5.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.568
Rodwell 12.735
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.457
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.237
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2929
0
2929
930471
33
3018
317.7
34.15
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.035 ± 0.058
0.63 ± 0.013
6.415 ± 0.04
6.206 ± 0.045
2.942 ± 0.025
8.865 ± 0.038
2.133 ± 0.022
4.193 ± 0.03
1.906 ± 0.03
10.176 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.895 ± 0.018
1.79 ± 0.023
5.349 ± 0.027
2.912 ± 0.024
7.676 ± 0.05
5.256 ± 0.026
5.891 ± 0.035
9.335 ± 0.045
1.459 ± 0.019
1.933 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here