Caudovirales sp.
Average proteome isoelectric point is 7.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 40 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A345MKJ4|A0A345MKJ4_9CAUD Uncharacterized protein OS=Caudovirales sp. OX=2202568 PE=4 SV=1
MM1 pKa = 7.88 TDD3 pKa = 3.01 TNATTTAAEE12 pKa = 4.38 TTTATTEE19 pKa = 3.96 AANTTTANTLLGGIGEE35 pKa = 4.32 QSEE38 pKa = 4.23 QAAGSLLGQQQGAAEE53 pKa = 4.45 EE54 pKa = 4.67 TTTTTPEE61 pKa = 3.91 RR62 pKa = 11.84 YY63 pKa = 9.4 VFPDD67 pKa = 3.25 KK68 pKa = 11.02 FQVKK72 pKa = 10.35 AGDD75 pKa = 4.19 DD76 pKa = 3.58 VDD78 pKa = 5.23 FDD80 pKa = 4.35 ASVRR84 pKa = 11.84 KK85 pKa = 10.05 LGEE88 pKa = 4.01 AYY90 pKa = 9.13 TNLEE94 pKa = 3.85 KK95 pKa = 10.92 RR96 pKa = 11.84 FGAGEE101 pKa = 3.97 ARR103 pKa = 11.84 PADD106 pKa = 3.58 VNGYY110 pKa = 10.07 KK111 pKa = 9.93 FDD113 pKa = 3.7 EE114 pKa = 4.54 SFGEE118 pKa = 4.53 GFQEE122 pKa = 5.79 AFTKK126 pKa = 10.7 DD127 pKa = 3.39 PNGQEE132 pKa = 4.0 WLKK135 pKa = 10.23 QAHH138 pKa = 5.91 EE139 pKa = 4.66 LGLNNQQVNFFMKK152 pKa = 10.25 EE153 pKa = 3.67 MIAAQPSTAEE163 pKa = 3.91 KK164 pKa = 7.4 TTGYY168 pKa = 9.12 TEE170 pKa = 3.99 AQAAEE175 pKa = 4.39 LLQKK179 pKa = 9.88 EE180 pKa = 4.25 WSEE183 pKa = 3.9 PAVYY187 pKa = 9.46 QAKK190 pKa = 10.53 LNAADD195 pKa = 3.88 RR196 pKa = 11.84 AARR199 pKa = 11.84 NLLKK203 pKa = 10.53 GDD205 pKa = 3.5 YY206 pKa = 9.28 QQFIGRR212 pKa = 11.84 YY213 pKa = 8.35 GNDD216 pKa = 3.31 PQVIKK221 pKa = 10.87 LLAAVGSEE229 pKa = 3.86 MSEE232 pKa = 3.94 DD233 pKa = 3.76 ALRR236 pKa = 11.84 LSGMPQLTADD246 pKa = 4.09 SLDD249 pKa = 3.28 DD250 pKa = 3.89 LMRR253 pKa = 11.84 SEE255 pKa = 5.12 AYY257 pKa = 10.25 NDD259 pKa = 3.57 EE260 pKa = 4.01 KK261 pKa = 11.2 HH262 pKa = 6.28 PDD264 pKa = 3.22 HH265 pKa = 7.03 RR266 pKa = 11.84 RR267 pKa = 11.84 VSQQVQDD274 pKa = 3.67 FFKK277 pKa = 10.53 RR278 pKa = 11.84 QYY280 pKa = 8.73 GTQEE284 pKa = 4.04 VII286 pKa = 3.63
Molecular weight: 31.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.654
IPC2_protein 4.571
IPC_protein 4.507
Toseland 4.355
ProMoST 4.609
Dawson 4.444
Bjellqvist 4.596
Wikipedia 4.304
Rodwell 4.342
Grimsley 4.266
Solomon 4.444
Lehninger 4.393
Nozaki 4.558
DTASelect 4.698
Thurlkill 4.342
EMBOSS 4.329
Sillero 4.609
Patrickios 4.05
IPC_peptide 4.457
IPC2_peptide 4.609
IPC2.peptide.svr19 4.552
Protein with the highest isoelectric point:
>tr|A0A345MKK0|A0A345MKK0_9CAUD Uncharacterized protein OS=Caudovirales sp. OX=2202568 PE=4 SV=1
MM1 pKa = 7.9 DD2 pKa = 3.85 TTSDD6 pKa = 3.26 LPYY9 pKa = 9.95 AINMLHH15 pKa = 6.14 STGQSAGSANSTPSSVAMRR34 pKa = 11.84 CASCTSSQVYY44 pKa = 9.43 GASFLMLRR52 pKa = 11.84 FVSWWMGLQKK62 pKa = 10.63 KK63 pKa = 10.0 SISALPRR70 pKa = 11.84 LSFASSSGSPSATHH84 pKa = 6.38 RR85 pKa = 11.84 RR86 pKa = 11.84 ARR88 pKa = 11.84 PAGG91 pKa = 3.52
Molecular weight: 9.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.341
IPC2_protein 9.736
IPC_protein 10.613
Toseland 10.496
ProMoST 10.292
Dawson 10.657
Bjellqvist 10.423
Wikipedia 10.891
Rodwell 10.804
Grimsley 10.73
Solomon 10.774
Lehninger 10.745
Nozaki 10.54
DTASelect 10.409
Thurlkill 10.54
EMBOSS 10.906
Sillero 10.599
Patrickios 10.657
IPC_peptide 10.774
IPC2_peptide 9.765
IPC2.peptide.svr19 8.283
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
40
0
40
12350
80
858
308.8
33.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.291 ± 0.731
1.441 ± 0.254
5.587 ± 0.274
4.826 ± 0.419
3.247 ± 0.208
8.065 ± 0.44
1.911 ± 0.183
4.308 ± 0.214
4.478 ± 0.33
7.822 ± 0.273
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.656 ± 0.245
4.364 ± 0.347
4.113 ± 0.21
5.587 ± 0.472
5.895 ± 0.294
6.162 ± 0.409
6.413 ± 0.368
6.47 ± 0.286
1.611 ± 0.152
2.753 ± 0.196
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here