Amino acid dipepetide frequency for Caudovirales sp.

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.326AlaAla: 20.326 ± 3.109
1.377AlaCys: 1.377 ± 0.453
7.126AlaAsp: 7.126 ± 0.757
6.397AlaGlu: 6.397 ± 0.875
2.834AlaPhe: 2.834 ± 0.463
9.07AlaGly: 9.07 ± 1.028
2.267AlaHis: 2.267 ± 0.507
5.264AlaIle: 5.264 ± 0.635
4.697AlaLys: 4.697 ± 0.707
8.422AlaLeu: 8.422 ± 1.123
3.482AlaMet: 3.482 ± 0.537
4.454AlaAsn: 4.454 ± 0.786
4.535AlaPro: 4.535 ± 0.641
8.989AlaGln: 8.989 ± 1.148
6.073AlaArg: 6.073 ± 0.941
7.531AlaSer: 7.531 ± 1.139
7.774AlaThr: 7.774 ± 1.146
7.45AlaVal: 7.45 ± 0.792
2.186AlaTrp: 2.186 ± 0.447
2.672AlaTyr: 2.672 ± 0.348
0.0AlaXaa: 0.0 ± 0.0
Cys
1.458CysAla: 1.458 ± 0.424
0.081CysCys: 0.081 ± 0.089
0.648CysAsp: 0.648 ± 0.218
0.729CysGlu: 0.729 ± 0.341
0.729CysPhe: 0.729 ± 0.196
0.972CysGly: 0.972 ± 0.277
0.324CysHis: 0.324 ± 0.228
0.729CysIle: 0.729 ± 0.234
0.486CysLys: 0.486 ± 0.181
0.405CysLeu: 0.405 ± 0.215
0.162CysMet: 0.162 ± 0.114
0.729CysAsn: 0.729 ± 0.25
0.972CysPro: 0.972 ± 0.406
0.486CysGln: 0.486 ± 0.2
1.296CysArg: 1.296 ± 0.429
0.81CysSer: 0.81 ± 0.311
0.972CysThr: 0.972 ± 0.275
1.296CysVal: 1.296 ± 0.404
0.567CysTrp: 0.567 ± 0.243
0.567CysTyr: 0.567 ± 0.308
0.0CysXaa: 0.0 ± 0.0
Asp
7.369AspAla: 7.369 ± 0.892
0.81AspCys: 0.81 ± 0.356
2.915AspAsp: 2.915 ± 0.608
3.158AspGlu: 3.158 ± 0.582
2.672AspPhe: 2.672 ± 0.518
3.968AspGly: 3.968 ± 0.655
0.972AspHis: 0.972 ± 0.294
2.672AspIle: 2.672 ± 0.439
2.348AspLys: 2.348 ± 0.421
5.021AspLeu: 5.021 ± 0.689
1.458AspMet: 1.458 ± 0.386
1.943AspAsn: 1.943 ± 0.353
1.943AspPro: 1.943 ± 0.463
2.186AspGln: 2.186 ± 0.317
2.51AspArg: 2.51 ± 0.437
3.239AspSer: 3.239 ± 0.547
3.482AspThr: 3.482 ± 0.654
3.887AspVal: 3.887 ± 0.487
1.458AspTrp: 1.458 ± 0.37
1.862AspTyr: 1.862 ± 0.367
0.0AspXaa: 0.0 ± 0.0
Glu
5.021GluAla: 5.021 ± 0.917
1.053GluCys: 1.053 ± 0.33
2.348GluAsp: 2.348 ± 0.444
2.186GluGlu: 2.186 ± 0.644
1.782GluPhe: 1.782 ± 0.411
3.32GluGly: 3.32 ± 0.599
1.701GluHis: 1.701 ± 0.41
2.753GluIle: 2.753 ± 0.49
2.429GluLys: 2.429 ± 0.457
4.373GluLeu: 4.373 ± 0.596
1.134GluMet: 1.134 ± 0.285
1.862GluAsn: 1.862 ± 0.419
1.62GluPro: 1.62 ± 0.478
3.563GluGln: 3.563 ± 0.598
3.725GluArg: 3.725 ± 0.651
2.105GluSer: 2.105 ± 0.422
2.024GluThr: 2.024 ± 0.4
2.834GluVal: 2.834 ± 0.448
0.729GluTrp: 0.729 ± 0.256
1.701GluTyr: 1.701 ± 0.558
0.0GluXaa: 0.0 ± 0.0
Phe
3.239PheAla: 3.239 ± 0.576
0.729PheCys: 0.729 ± 0.295
2.348PheAsp: 2.348 ± 0.431
1.134PheGlu: 1.134 ± 0.346
0.972PhePhe: 0.972 ± 0.249
3.401PheGly: 3.401 ± 0.499
0.972PheHis: 0.972 ± 0.269
1.862PheIle: 1.862 ± 0.286
1.458PheLys: 1.458 ± 0.352
1.782PheLeu: 1.782 ± 0.395
0.891PheMet: 0.891 ± 0.254
2.105PheAsn: 2.105 ± 0.482
1.215PhePro: 1.215 ± 0.263
1.377PheGln: 1.377 ± 0.313
1.539PheArg: 1.539 ± 0.379
1.539PheSer: 1.539 ± 0.443
2.51PheThr: 2.51 ± 0.431
1.943PheVal: 1.943 ± 0.351
0.405PheTrp: 0.405 ± 0.16
1.053PheTyr: 1.053 ± 0.289
0.0PheXaa: 0.0 ± 0.0
Gly
8.422GlyAla: 8.422 ± 1.34
0.891GlyCys: 0.891 ± 0.292
4.211GlyAsp: 4.211 ± 0.5
3.887GlyGlu: 3.887 ± 0.487
3.32GlyPhe: 3.32 ± 0.527
7.936GlyGly: 7.936 ± 2.033
1.215GlyHis: 1.215 ± 0.28
3.077GlyIle: 3.077 ± 0.437
3.887GlyLys: 3.887 ± 0.666
6.235GlyLeu: 6.235 ± 0.584
2.429GlyMet: 2.429 ± 0.573
3.563GlyAsn: 3.563 ± 1.048
1.782GlyPro: 1.782 ± 0.389
3.725GlyGln: 3.725 ± 0.706
5.021GlyArg: 5.021 ± 0.646
5.911GlySer: 5.911 ± 1.002
5.102GlyThr: 5.102 ± 0.644
6.397GlyVal: 6.397 ± 0.538
1.62GlyTrp: 1.62 ± 0.393
2.024GlyTyr: 2.024 ± 0.351
0.0GlyXaa: 0.0 ± 0.0
His
1.62HisAla: 1.62 ± 0.372
0.243HisCys: 0.243 ± 0.18
0.972HisAsp: 0.972 ± 0.293
0.729HisGlu: 0.729 ± 0.24
0.486HisPhe: 0.486 ± 0.188
1.862HisGly: 1.862 ± 0.497
0.648HisHis: 0.648 ± 0.28
0.648HisIle: 0.648 ± 0.168
0.648HisLys: 0.648 ± 0.219
1.862HisLeu: 1.862 ± 0.389
0.567HisMet: 0.567 ± 0.28
0.405HisAsn: 0.405 ± 0.198
1.134HisPro: 1.134 ± 0.4
0.729HisGln: 0.729 ± 0.212
1.296HisArg: 1.296 ± 0.322
1.62HisSer: 1.62 ± 0.335
1.215HisThr: 1.215 ± 0.243
1.134HisVal: 1.134 ± 0.351
0.486HisTrp: 0.486 ± 0.21
0.81HisTyr: 0.81 ± 0.204
0.0HisXaa: 0.0 ± 0.0
Ile
6.235IleAla: 6.235 ± 0.962
0.486IleCys: 0.486 ± 0.214
3.239IleAsp: 3.239 ± 0.652
2.348IleGlu: 2.348 ± 0.492
0.81IlePhe: 0.81 ± 0.254
4.373IleGly: 4.373 ± 0.632
1.377IleHis: 1.377 ± 0.331
1.377IleIle: 1.377 ± 0.348
1.782IleLys: 1.782 ± 0.322
2.105IleLeu: 2.105 ± 0.464
0.891IleMet: 0.891 ± 0.276
2.51IleAsn: 2.51 ± 0.534
1.862IlePro: 1.862 ± 0.343
1.296IleGln: 1.296 ± 0.349
2.024IleArg: 2.024 ± 0.37
2.267IleSer: 2.267 ± 0.49
3.644IleThr: 3.644 ± 0.434
2.429IleVal: 2.429 ± 0.569
0.486IleTrp: 0.486 ± 0.158
1.539IleTyr: 1.539 ± 0.312
0.0IleXaa: 0.0 ± 0.0
Lys
4.616LysAla: 4.616 ± 0.588
0.648LysCys: 0.648 ± 0.24
2.348LysAsp: 2.348 ± 0.456
2.429LysGlu: 2.429 ± 0.574
1.134LysPhe: 1.134 ± 0.358
2.996LysGly: 2.996 ± 0.469
0.81LysHis: 0.81 ± 0.219
1.539LysIle: 1.539 ± 0.394
2.105LysLys: 2.105 ± 0.361
3.725LysLeu: 3.725 ± 0.524
1.62LysMet: 1.62 ± 0.35
1.943LysAsn: 1.943 ± 0.396
2.186LysPro: 2.186 ± 0.404
2.834LysGln: 2.834 ± 0.544
2.672LysArg: 2.672 ± 0.481
2.429LysSer: 2.429 ± 0.371
3.077LysThr: 3.077 ± 0.713
3.725LysVal: 3.725 ± 0.533
0.567LysTrp: 0.567 ± 0.258
1.296LysTyr: 1.296 ± 0.326
0.0LysXaa: 0.0 ± 0.0
Leu
9.07LeuAla: 9.07 ± 0.834
1.134LeuCys: 1.134 ± 0.358
4.535LeuAsp: 4.535 ± 0.58
3.644LeuGlu: 3.644 ± 0.562
2.915LeuPhe: 2.915 ± 0.538
5.911LeuGly: 5.911 ± 0.882
1.458LeuHis: 1.458 ± 0.32
3.158LeuIle: 3.158 ± 0.538
3.32LeuLys: 3.32 ± 0.504
6.073LeuLeu: 6.073 ± 0.759
2.024LeuMet: 2.024 ± 0.363
4.049LeuAsn: 4.049 ± 0.738
3.482LeuPro: 3.482 ± 0.584
3.968LeuGln: 3.968 ± 0.617
6.721LeuArg: 6.721 ± 0.994
4.292LeuSer: 4.292 ± 0.638
5.345LeuThr: 5.345 ± 0.713
4.211LeuVal: 4.211 ± 0.627
0.891LeuTrp: 0.891 ± 0.351
2.024LeuTyr: 2.024 ± 0.443
0.0LeuXaa: 0.0 ± 0.0
Met
2.753MetAla: 2.753 ± 0.539
0.243MetCys: 0.243 ± 0.167
0.81MetAsp: 0.81 ± 0.23
0.648MetGlu: 0.648 ± 0.207
1.053MetPhe: 1.053 ± 0.347
1.458MetGly: 1.458 ± 0.401
0.567MetHis: 0.567 ± 0.264
0.972MetIle: 0.972 ± 0.269
1.134MetLys: 1.134 ± 0.329
2.51MetLeu: 2.51 ± 0.365
0.567MetMet: 0.567 ± 0.247
1.458MetAsn: 1.458 ± 0.349
0.972MetPro: 0.972 ± 0.262
1.782MetGln: 1.782 ± 0.448
1.943MetArg: 1.943 ± 0.394
2.429MetSer: 2.429 ± 0.424
1.377MetThr: 1.377 ± 0.323
2.429MetVal: 2.429 ± 0.43
0.486MetTrp: 0.486 ± 0.191
0.972MetTyr: 0.972 ± 0.288
0.0MetXaa: 0.0 ± 0.0
Asn
6.073AsnAla: 6.073 ± 1.141
0.567AsnCys: 0.567 ± 0.197
2.834AsnAsp: 2.834 ± 0.467
2.105AsnGlu: 2.105 ± 0.413
1.134AsnPhe: 1.134 ± 0.337
5.183AsnGly: 5.183 ± 1.288
0.405AsnHis: 0.405 ± 0.165
2.51AsnIle: 2.51 ± 0.52
1.862AsnLys: 1.862 ± 0.501
1.782AsnLeu: 1.782 ± 0.303
1.134AsnMet: 1.134 ± 0.238
1.701AsnAsn: 1.701 ± 0.51
2.834AsnPro: 2.834 ± 0.41
2.267AsnGln: 2.267 ± 0.331
1.862AsnArg: 1.862 ± 0.352
2.834AsnSer: 2.834 ± 0.612
2.672AsnThr: 2.672 ± 0.76
2.672AsnVal: 2.672 ± 0.573
0.243AsnTrp: 0.243 ± 0.136
0.972AsnTyr: 0.972 ± 0.189
0.0AsnXaa: 0.0 ± 0.0
Pro
5.183ProAla: 5.183 ± 0.744
0.648ProCys: 0.648 ± 0.233
2.591ProAsp: 2.591 ± 0.516
2.51ProGlu: 2.51 ± 0.507
1.134ProPhe: 1.134 ± 0.248
3.563ProGly: 3.563 ± 0.645
0.81ProHis: 0.81 ± 0.281
1.539ProIle: 1.539 ± 0.314
1.62ProLys: 1.62 ± 0.473
3.482ProLeu: 3.482 ± 0.55
0.81ProMet: 0.81 ± 0.282
1.215ProAsn: 1.215 ± 0.394
0.972ProPro: 0.972 ± 0.236
1.782ProGln: 1.782 ± 0.316
3.077ProArg: 3.077 ± 0.594
2.591ProSer: 2.591 ± 0.433
2.915ProThr: 2.915 ± 0.543
2.915ProVal: 2.915 ± 0.428
0.243ProTrp: 0.243 ± 0.119
1.539ProTyr: 1.539 ± 0.305
0.0ProXaa: 0.0 ± 0.0
Gln
6.478GlnAla: 6.478 ± 1.163
0.648GlnCys: 0.648 ± 0.249
2.672GlnAsp: 2.672 ± 0.625
1.862GlnGlu: 1.862 ± 0.353
2.105GlnPhe: 2.105 ± 0.4
3.482GlnGly: 3.482 ± 0.544
0.891GlnHis: 0.891 ± 0.349
1.943GlnIle: 1.943 ± 0.379
2.267GlnLys: 2.267 ± 0.433
4.859GlnLeu: 4.859 ± 0.649
1.701GlnMet: 1.701 ± 0.33
2.834GlnAsn: 2.834 ± 0.496
2.834GlnPro: 2.834 ± 0.596
5.587GlnGln: 5.587 ± 1.197
3.644GlnArg: 3.644 ± 0.587
3.077GlnSer: 3.077 ± 0.629
3.563GlnThr: 3.563 ± 0.612
3.563GlnVal: 3.563 ± 0.625
1.053GlnTrp: 1.053 ± 0.233
0.81GlnTyr: 0.81 ± 0.25
0.0GlnXaa: 0.0 ± 0.0
Arg
6.073ArgAla: 6.073 ± 0.874
1.782ArgCys: 1.782 ± 0.506
3.239ArgAsp: 3.239 ± 0.505
3.644ArgGlu: 3.644 ± 0.589
1.943ArgPhe: 1.943 ± 0.436
3.725ArgGly: 3.725 ± 0.669
0.648ArgHis: 0.648 ± 0.235
2.834ArgIle: 2.834 ± 0.351
3.32ArgLys: 3.32 ± 0.548
5.911ArgLeu: 5.911 ± 0.761
1.62ArgMet: 1.62 ± 0.273
2.591ArgAsn: 2.591 ± 0.359
2.672ArgPro: 2.672 ± 0.575
3.32ArgGln: 3.32 ± 0.68
3.806ArgArg: 3.806 ± 0.628
3.158ArgSer: 3.158 ± 0.526
2.672ArgThr: 2.672 ± 0.493
3.32ArgVal: 3.32 ± 0.592
0.891ArgTrp: 0.891 ± 0.269
1.782ArgTyr: 1.782 ± 0.293
0.0ArgXaa: 0.0 ± 0.0
Ser
8.26SerAla: 8.26 ± 1.11
0.729SerCys: 0.729 ± 0.236
3.158SerAsp: 3.158 ± 0.415
3.239SerGlu: 3.239 ± 0.504
1.862SerPhe: 1.862 ± 0.324
5.668SerGly: 5.668 ± 1.124
0.81SerHis: 0.81 ± 0.231
2.591SerIle: 2.591 ± 0.355
2.996SerLys: 2.996 ± 0.518
4.94SerLeu: 4.94 ± 0.596
1.862SerMet: 1.862 ± 0.38
2.672SerAsn: 2.672 ± 0.499
2.348SerPro: 2.348 ± 0.51
3.563SerGln: 3.563 ± 0.742
3.077SerArg: 3.077 ± 0.468
4.13SerSer: 4.13 ± 0.672
3.644SerThr: 3.644 ± 0.69
3.806SerVal: 3.806 ± 0.469
0.972SerTrp: 0.972 ± 0.378
1.296SerTyr: 1.296 ± 0.273
0.0SerXaa: 0.0 ± 0.0
Thr
8.989ThrAla: 8.989 ± 1.114
0.567ThrCys: 0.567 ± 0.238
3.806ThrAsp: 3.806 ± 0.503
2.429ThrGlu: 2.429 ± 0.486
1.943ThrPhe: 1.943 ± 0.347
6.154ThrGly: 6.154 ± 0.557
0.648ThrHis: 0.648 ± 0.221
2.753ThrIle: 2.753 ± 0.544
3.239ThrLys: 3.239 ± 0.493
4.859ThrLeu: 4.859 ± 0.67
1.62ThrMet: 1.62 ± 0.323
2.429ThrAsn: 2.429 ± 0.573
2.915ThrPro: 2.915 ± 0.469
3.077ThrGln: 3.077 ± 0.654
2.672ThrArg: 2.672 ± 0.56
4.616ThrSer: 4.616 ± 0.789
4.778ThrThr: 4.778 ± 1.101
3.968ThrVal: 3.968 ± 0.706
0.891ThrTrp: 0.891 ± 0.228
1.782ThrTyr: 1.782 ± 0.498
0.0ThrXaa: 0.0 ± 0.0
Val
7.774ValAla: 7.774 ± 0.871
0.729ValCys: 0.729 ± 0.282
3.725ValAsp: 3.725 ± 0.547
3.158ValGlu: 3.158 ± 0.57
1.862ValPhe: 1.862 ± 0.345
4.859ValGly: 4.859 ± 0.603
1.701ValHis: 1.701 ± 0.333
3.077ValIle: 3.077 ± 0.421
2.996ValLys: 2.996 ± 0.369
5.749ValLeu: 5.749 ± 0.811
1.134ValMet: 1.134 ± 0.298
3.563ValAsn: 3.563 ± 0.598
2.834ValPro: 2.834 ± 0.415
3.077ValGln: 3.077 ± 0.515
3.644ValArg: 3.644 ± 0.514
4.292ValSer: 4.292 ± 0.521
4.049ValThr: 4.049 ± 0.791
3.806ValVal: 3.806 ± 0.671
0.972ValTrp: 0.972 ± 0.23
1.701ValTyr: 1.701 ± 0.326
0.0ValXaa: 0.0 ± 0.0
Trp
1.458TrpAla: 1.458 ± 0.353
0.729TrpCys: 0.729 ± 0.288
0.729TrpAsp: 0.729 ± 0.247
0.729TrpGlu: 0.729 ± 0.249
0.729TrpPhe: 0.729 ± 0.276
0.405TrpGly: 0.405 ± 0.178
0.081TrpHis: 0.081 ± 0.084
0.972TrpIle: 0.972 ± 0.315
0.891TrpLys: 0.891 ± 0.249
2.348TrpLeu: 2.348 ± 0.433
0.405TrpMet: 0.405 ± 0.193
0.486TrpAsn: 0.486 ± 0.152
0.972TrpPro: 0.972 ± 0.314
0.729TrpGln: 0.729 ± 0.215
0.972TrpArg: 0.972 ± 0.355
1.134TrpSer: 1.134 ± 0.301
0.81TrpThr: 0.81 ± 0.211
0.729TrpVal: 0.729 ± 0.276
0.486TrpTrp: 0.486 ± 0.193
0.324TrpTyr: 0.324 ± 0.154
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.51TyrAla: 2.51 ± 0.426
0.324TyrCys: 0.324 ± 0.213
1.62TyrAsp: 1.62 ± 0.364
1.296TyrGlu: 1.296 ± 0.31
1.053TyrPhe: 1.053 ± 0.272
2.348TyrGly: 2.348 ± 0.412
0.81TyrHis: 0.81 ± 0.323
0.81TyrIle: 0.81 ± 0.27
1.296TyrLys: 1.296 ± 0.284
1.782TyrLeu: 1.782 ± 0.434
0.972TyrMet: 0.972 ± 0.289
1.134TyrAsn: 1.134 ± 0.301
1.053TyrPro: 1.053 ± 0.353
1.539TyrGln: 1.539 ± 0.305
1.377TyrArg: 1.377 ± 0.352
1.62TyrSer: 1.62 ± 0.39
2.51TyrThr: 2.51 ± 0.376
2.186TyrVal: 2.186 ± 0.388
0.486TyrTrp: 0.486 ± 0.211
0.81TyrTyr: 0.81 ± 0.264
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 40 proteins (12350 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski