Leptotrichia goodfellowii F0264
Average proteome isoelectric point is 6.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2374 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D0GN21|D0GN21_9FUSO Uncharacterized protein OS=Leptotrichia goodfellowii F0264 OX=596323 GN=HMPREF0554_1168 PE=4 SV=1
MM1 pKa = 7.1 KK2 pKa = 10.14 QYY4 pKa = 11.31 AGTGYY9 pKa = 10.39 DD10 pKa = 3.08 RR11 pKa = 11.84 KK12 pKa = 10.62 YY13 pKa = 10.31 EE14 pKa = 3.98 AAASDD19 pKa = 3.81 WRR21 pKa = 11.84 DD22 pKa = 3.27 KK23 pKa = 11.39 QYY25 pKa = 11.76 DD26 pKa = 4.22 DD27 pKa = 3.77 YY28 pKa = 11.78 CAAQDD33 pKa = 3.62 EE34 pKa = 4.79 YY35 pKa = 11.69 DD36 pKa = 3.57 RR37 pKa = 11.84 QIQEE41 pKa = 3.93 KK42 pKa = 10.46 FEE44 pKa = 4.15 EE45 pKa = 4.55 LFNEE49 pKa = 4.14 LEE51 pKa = 4.54 VYY53 pKa = 7.92 HH54 pKa = 6.24 TSYY57 pKa = 11.24 YY58 pKa = 11.12 DD59 pKa = 3.41 KK60 pKa = 10.97 LSEE63 pKa = 4.39 NEE65 pKa = 3.9 WIEE68 pKa = 3.88 LKK70 pKa = 10.76 DD71 pKa = 3.49 YY72 pKa = 10.93 FRR74 pKa = 11.84 DD75 pKa = 3.57 YY76 pKa = 11.0 EE77 pKa = 4.51 YY78 pKa = 11.51 NLEE81 pKa = 4.3 TTFSDD86 pKa = 4.03 VIEE89 pKa = 4.72 SYY91 pKa = 10.5 QEE93 pKa = 3.8 EE94 pKa = 4.93 LIEE97 pKa = 4.97 KK98 pKa = 8.95 YY99 pKa = 11.25 NFMII103 pKa = 5.17
Molecular weight: 12.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.952
IPC2_protein 4.164
IPC_protein 4.088
Toseland 3.91
ProMoST 4.215
Dawson 4.05
Bjellqvist 4.202
Wikipedia 3.923
Rodwell 3.923
Grimsley 3.821
Solomon 4.037
Lehninger 3.986
Nozaki 4.151
DTASelect 4.304
Thurlkill 3.935
EMBOSS 3.935
Sillero 4.202
Patrickios 0.629
IPC_peptide 4.037
IPC2_peptide 4.19
IPC2.peptide.svr19 4.095
Protein with the highest isoelectric point:
>tr|D0GJ94|D0GJ94_9FUSO Carbamate kinase OS=Leptotrichia goodfellowii F0264 OX=596323 GN=arcC PE=3 SV=1
MM1 pKa = 7.65 AVPKK5 pKa = 10.55 KK6 pKa = 9.29 RR7 pKa = 11.84 TSKK10 pKa = 10.63 AKK12 pKa = 9.33 RR13 pKa = 11.84 NMRR16 pKa = 11.84 RR17 pKa = 11.84 AHH19 pKa = 6.89 DD20 pKa = 4.44 SIKK23 pKa = 10.61 APNIIVEE30 pKa = 4.06 ADD32 pKa = 3.3 GTVRR36 pKa = 11.84 RR37 pKa = 11.84 PHH39 pKa = 6.69 RR40 pKa = 11.84 LNLEE44 pKa = 3.86 TGVYY48 pKa = 9.97 RR49 pKa = 11.84 GRR51 pKa = 11.84 QVLSTEE57 pKa = 4.21 SPAADD62 pKa = 3.44 SEE64 pKa = 4.52
Molecular weight: 7.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.311
IPC2_protein 9.736
IPC_protein 10.745
Toseland 11.082
ProMoST 11.067
Dawson 11.125
Bjellqvist 10.906
Wikipedia 11.403
Rodwell 11.257
Grimsley 11.155
Solomon 11.389
Lehninger 11.33
Nozaki 11.067
DTASelect 10.906
Thurlkill 11.067
EMBOSS 11.52
Sillero 11.067
Patrickios 11.023
IPC_peptide 11.389
IPC2_peptide 9.926
IPC2.peptide.svr19 8.835
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2374
0
2374
685734
32
2432
288.9
32.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.286 ± 0.058
0.646 ± 0.012
5.217 ± 0.036
8.317 ± 0.073
4.775 ± 0.051
6.673 ± 0.067
1.247 ± 0.021
9.481 ± 0.065
10.379 ± 0.065
8.713 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.512 ± 0.027
6.319 ± 0.056
2.686 ± 0.029
2.243 ± 0.021
3.355 ± 0.031
5.839 ± 0.038
4.994 ± 0.043
6.282 ± 0.044
0.728 ± 0.018
4.31 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here