Takifugu rubripes (Japanese pufferfish) (Fugu rubripes)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala;

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51089 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A674MWN5|A0A674MWN5_TAKRU Thioredoxin domain-containing protein OS=Takifugu rubripes OX=31033 PE=4 SV=1
TT1 pKa = 7.31GSFSYY6 pKa = 10.67SCTQSHH12 pKa = 5.09VHH14 pKa = 5.19AHH16 pKa = 5.25ITRR19 pKa = 11.84SLWEE23 pKa = 4.02AVSNKK28 pKa = 9.8QKK30 pKa = 10.68DD31 pKa = 3.43RR32 pKa = 11.84CPPAEE37 pKa = 4.4EE38 pKa = 5.52DD39 pKa = 3.15ICPPADD45 pKa = 3.86EE46 pKa = 4.89EE47 pKa = 4.4RR48 pKa = 11.84CPPADD53 pKa = 4.59EE54 pKa = 5.79DD55 pKa = 3.67ICPPADD61 pKa = 4.15EE62 pKa = 5.89DD63 pKa = 3.63ICPPADD69 pKa = 3.93EE70 pKa = 5.32DD71 pKa = 3.81RR72 pKa = 11.84CPPADD77 pKa = 4.57EE78 pKa = 5.82DD79 pKa = 3.67ICPPADD85 pKa = 3.93EE86 pKa = 5.32DD87 pKa = 3.81RR88 pKa = 11.84CPPADD93 pKa = 4.33EE94 pKa = 5.22DD95 pKa = 3.84RR96 pKa = 11.84CPPADD101 pKa = 4.33EE102 pKa = 5.22DD103 pKa = 3.84RR104 pKa = 11.84CPPADD109 pKa = 4.33EE110 pKa = 5.22DD111 pKa = 3.84RR112 pKa = 11.84CPPADD117 pKa = 4.57EE118 pKa = 5.82DD119 pKa = 3.67ICPPADD125 pKa = 3.85EE126 pKa = 6.11DD127 pKa = 3.62ISPPADD133 pKa = 3.55EE134 pKa = 6.02DD135 pKa = 3.45ICPPADD141 pKa = 4.11EE142 pKa = 5.6DD143 pKa = 4.02RR144 pKa = 11.84CCQII148 pKa = 4.33

Molecular weight: 16.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A674PBD4|A0A674PBD4_TAKRU Uncharacterized protein OS=Takifugu rubripes OX=31033 PE=4 SV=1
RR1 pKa = 8.73VDD3 pKa = 3.49ILNGLLAGSNRR14 pKa = 11.84QGGNRR19 pKa = 11.84PGGNRR24 pKa = 11.84QGGKK28 pKa = 8.9RR29 pKa = 11.84QGGNRR34 pKa = 11.84QGGNRR39 pKa = 11.84QGGNRR44 pKa = 11.84RR45 pKa = 11.84GGNRR49 pKa = 11.84PGGKK53 pKa = 9.25RR54 pKa = 11.84PGGNRR59 pKa = 11.84PGGKK63 pKa = 9.33RR64 pKa = 11.84PGGNRR69 pKa = 11.84QGGNRR74 pKa = 11.84PGGKK78 pKa = 9.25RR79 pKa = 11.84PGGNRR84 pKa = 11.84PGGKK88 pKa = 9.25RR89 pKa = 11.84PGGNRR94 pKa = 11.84PGGNRR99 pKa = 11.84QGGNRR104 pKa = 11.84PGGNRR109 pKa = 11.84QGGKK113 pKa = 8.9RR114 pKa = 11.84QGGNRR119 pKa = 11.84QGGNRR124 pKa = 11.84RR125 pKa = 11.84GGNRR129 pKa = 11.84PGGKK133 pKa = 9.25RR134 pKa = 11.84PGGNRR139 pKa = 11.84PGGKK143 pKa = 9.25RR144 pKa = 11.84PGGNRR149 pKa = 11.84PGGLGFRR156 pKa = 11.84VRR158 pKa = 11.84VRR160 pKa = 11.84VRR162 pKa = 11.84VRR164 pKa = 11.84VSQDD168 pKa = 2.74RR169 pKa = 11.84PVLSNHH175 pKa = 5.31TVGG178 pKa = 4.93

Molecular weight: 18.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

21256

29833

51089

32382901

18

7643

633.9

70.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.55 ± 0.009

2.29 ± 0.008

5.179 ± 0.007

6.659 ± 0.012

3.781 ± 0.007

6.345 ± 0.014

2.692 ± 0.006

4.564 ± 0.008

5.526 ± 0.009

9.719 ± 0.013

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.386 ± 0.005

3.892 ± 0.006

5.625 ± 0.014

4.663 ± 0.007

5.648 ± 0.007

8.507 ± 0.012

5.544 ± 0.01

6.44 ± 0.008

1.185 ± 0.004

2.805 ± 0.006
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski