Amino acid dipepetide frequency for Takifugu rubripes (Japanese pufferfish) (Fugu rubripes)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.227AlaAla: 6.227 ± 0.024	1.326AlaCys: 1.326 ± 0.009	3.222AlaAsp: 3.222 ± 0.01	4.497AlaGlu: 4.497 ± 0.019	2.47AlaPhe: 2.47 ± 0.011	4.279AlaGly: 4.279 ± 0.018	1.516AlaHis: 1.516 ± 0.008	2.846AlaIle: 2.846 ± 0.011	3.291AlaLys: 3.291 ± 0.015	6.504AlaLeu: 6.504 ± 0.017	1.584AlaMet: 1.584 ± 0.009	2.219AlaAsn: 2.219 ± 0.009	3.491AlaPro: 3.491 ± 0.022	2.851AlaGln: 2.851 ± 0.013	3.177AlaArg: 3.177 ± 0.011	5.394AlaSer: 5.394 ± 0.021	3.483AlaThr: 3.483 ± 0.014	4.911AlaVal: 4.911 ± 0.014	0.66AlaTrp: 0.66 ± 0.005	1.556AlaTyr: 1.556 ± 0.007	0.0AlaXaa: 0.0 ± 0.0
Cys	1.212CysAla: 1.212 ± 0.008	0.671CysCys: 0.671 ± 0.006	1.129CysAsp: 1.129 ± 0.009	1.237CysGlu: 1.237 ± 0.009	0.943CysPhe: 0.943 ± 0.006	1.562CysGly: 1.562 ± 0.011	0.678CysHis: 0.678 ± 0.006	1.01CysIle: 1.01 ± 0.007	1.166CysLys: 1.166 ± 0.009	2.287CysLeu: 2.287 ± 0.014	0.475CysMet: 0.475 ± 0.005	0.85CysAsn: 0.85 ± 0.007	1.301CysPro: 1.301 ± 0.01	1.052CysGln: 1.052 ± 0.008	1.346CysArg: 1.346 ± 0.008	2.183CysSer: 2.183 ± 0.012	1.203CysThr: 1.203 ± 0.009	1.66CysVal: 1.66 ± 0.013	0.329CysTrp: 0.329 ± 0.004	0.609CysTyr: 0.609 ± 0.005	0.0CysXaa: 0.0 ± 0.0
Asp	3.015AspAla: 3.015 ± 0.011	1.143AspCys: 1.143 ± 0.008	2.971AspAsp: 2.971 ± 0.016	3.609AspGlu: 3.609 ± 0.012	2.194AspPhe: 2.194 ± 0.009	3.697AspGly: 3.697 ± 0.014	1.252AspHis: 1.252 ± 0.007	2.768AspIle: 2.768 ± 0.012	2.706AspLys: 2.706 ± 0.011	5.059AspLeu: 5.059 ± 0.016	1.277AspMet: 1.277 ± 0.006	1.981AspAsn: 1.981 ± 0.009	2.922AspPro: 2.922 ± 0.011	1.96AspGln: 1.96 ± 0.009	2.728AspArg: 2.728 ± 0.011	4.266AspSer: 4.266 ± 0.013	2.571AspThr: 2.571 ± 0.011	3.382AspVal: 3.382 ± 0.017	0.689AspTrp: 0.689 ± 0.006	1.599AspTyr: 1.599 ± 0.007	0.0AspXaa: 0.0 ± 0.0
Glu	4.621GluAla: 4.621 ± 0.026	1.263GluCys: 1.263 ± 0.009	4.26GluAsp: 4.26 ± 0.015	7.073GluGlu: 7.073 ± 0.036	2.014GluPhe: 2.014 ± 0.009	4.061GluGly: 4.061 ± 0.014	1.469GluHis: 1.469 ± 0.007	2.89GluIle: 2.89 ± 0.01	4.438GluLys: 4.438 ± 0.021	6.175GluLeu: 6.175 ± 0.024	1.746GluMet: 1.746 ± 0.009	2.743GluAsn: 2.743 ± 0.011	2.674GluPro: 2.674 ± 0.013	3.001GluGln: 3.001 ± 0.015	4.125GluArg: 4.125 ± 0.019	4.187GluSer: 4.187 ± 0.014	3.267GluThr: 3.267 ± 0.013	4.268GluVal: 4.268 ± 0.015	0.695GluTrp: 0.695 ± 0.006	1.623GluTyr: 1.623 ± 0.007	0.0GluXaa: 0.0 ± 0.0
Phe	2.028PheAla: 2.028 ± 0.009	0.982PheCys: 0.982 ± 0.007	1.822PheAsp: 1.822 ± 0.008	1.912PheGlu: 1.912 ± 0.008	1.728PhePhe: 1.728 ± 0.01	2.329PheGly: 2.329 ± 0.01	1.096PheHis: 1.096 ± 0.006	2.031PheIle: 2.031 ± 0.008	1.879PheLys: 1.879 ± 0.008	4.135PheLeu: 4.135 ± 0.016	0.875PheMet: 0.875 ± 0.006	1.555PheAsn: 1.555 ± 0.008	1.889PhePro: 1.889 ± 0.009	1.704PheGln: 1.704 ± 0.008	1.934PheArg: 1.934 ± 0.008	3.495PheSer: 3.495 ± 0.014	2.3PheThr: 2.3 ± 0.01	2.339PheVal: 2.339 ± 0.01	0.5PheTrp: 0.5 ± 0.004	1.276PheTyr: 1.276 ± 0.008	0.0PheXaa: 0.0 ± 0.0
Gly	4.105GlyAla: 4.105 ± 0.021	1.273GlyCys: 1.273 ± 0.008	3.24GlyAsp: 3.24 ± 0.013	3.979GlyGlu: 3.979 ± 0.017	2.542GlyPhe: 2.542 ± 0.014	5.221GlyGly: 5.221 ± 0.024	1.719GlyHis: 1.719 ± 0.009	2.733GlyIle: 2.733 ± 0.011	3.487GlyLys: 3.487 ± 0.011	5.557GlyLeu: 5.557 ± 0.02	1.473GlyMet: 1.473 ± 0.008	2.48GlyAsn: 2.48 ± 0.01	3.306GlyPro: 3.306 ± 0.03	2.745GlyGln: 2.745 ± 0.013	3.673GlyArg: 3.673 ± 0.015	5.639GlySer: 5.639 ± 0.021	3.528GlyThr: 3.528 ± 0.014	4.063GlyVal: 4.063 ± 0.014	0.791GlyTrp: 0.791 ± 0.006	1.893GlyTyr: 1.893 ± 0.01	0.0GlyXaa: 0.0 ± 0.0
His	1.401HisAla: 1.401 ± 0.008	0.791HisCys: 0.791 ± 0.007	1.001HisAsp: 1.001 ± 0.006	1.217HisGlu: 1.217 ± 0.007	1.114HisPhe: 1.114 ± 0.006	1.607HisGly: 1.607 ± 0.009	1.054HisHis: 1.054 ± 0.01	1.365HisIle: 1.365 ± 0.007	1.296HisLys: 1.296 ± 0.007	2.859HisLeu: 2.859 ± 0.014	0.651HisMet: 0.651 ± 0.005	1.034HisAsn: 1.034 ± 0.006	1.751HisPro: 1.751 ± 0.025	1.325HisGln: 1.325 ± 0.008	1.679HisArg: 1.679 ± 0.009	2.414HisSer: 2.414 ± 0.01	1.608HisThr: 1.608 ± 0.016	1.545HisVal: 1.545 ± 0.008	0.351HisTrp: 0.351 ± 0.004	0.859HisTyr: 0.859 ± 0.005	0.0HisXaa: 0.0 ± 0.0
Ile	2.564IleAla: 2.564 ± 0.01	1.108IleCys: 1.108 ± 0.008	2.166IleAsp: 2.166 ± 0.009	2.39IleGlu: 2.39 ± 0.01	1.993IlePhe: 1.993 ± 0.01	2.418IleGly: 2.418 ± 0.01	1.387IleHis: 1.387 ± 0.018	2.594IleIle: 2.594 ± 0.013	2.626IleLys: 2.626 ± 0.01	4.464IleLeu: 4.464 ± 0.013	1.125IleMet: 1.125 ± 0.006	2.066IleAsn: 2.066 ± 0.008	2.509IlePro: 2.509 ± 0.01	2.284IleGln: 2.284 ± 0.01	2.576IleArg: 2.576 ± 0.008	3.889IleSer: 3.889 ± 0.013	2.813IleThr: 2.813 ± 0.013	2.674IleVal: 2.674 ± 0.01	0.504IleTrp: 0.504 ± 0.004	1.485IleTyr: 1.485 ± 0.009	0.0IleXaa: 0.0 ± 0.0
Lys	3.616LysAla: 3.616 ± 0.013	1.055LysCys: 1.055 ± 0.007	3.218LysAsp: 3.218 ± 0.012	4.548LysGlu: 4.548 ± 0.022	1.662LysPhe: 1.662 ± 0.008	3.153LysGly: 3.153 ± 0.016	1.412LysHis: 1.412 ± 0.008	2.562LysIle: 2.562 ± 0.011	4.217LysLys: 4.217 ± 0.017	4.992LysLeu: 4.992 ± 0.015	1.485LysMet: 1.485 ± 0.008	2.307LysAsn: 2.307 ± 0.008	2.724LysPro: 2.724 ± 0.012	2.45LysGln: 2.45 ± 0.011	3.366LysArg: 3.366 ± 0.013	3.758LysSer: 3.758 ± 0.014	3.06LysThr: 3.06 ± 0.011	3.56LysVal: 3.56 ± 0.011	0.589LysTrp: 0.589 ± 0.004	1.524LysTyr: 1.524 ± 0.007	0.0LysXaa: 0.0 ± 0.0
Leu	6.157LeuAla: 6.157 ± 0.019	2.281LeuCys: 2.281 ± 0.012	5.005LeuAsp: 5.005 ± 0.015	6.455LeuGlu: 6.455 ± 0.026	3.628LeuPhe: 3.628 ± 0.013	5.26LeuGly: 5.26 ± 0.015	2.813LeuHis: 2.813 ± 0.013	4.003LeuIle: 4.003 ± 0.014	5.678LeuLys: 5.678 ± 0.017	10.371LeuLeu: 10.371 ± 0.029	2.231LeuMet: 2.231 ± 0.01	3.731LeuAsn: 3.731 ± 0.012	5.305LeuPro: 5.305 ± 0.018	5.484LeuGln: 5.484 ± 0.019	5.694LeuArg: 5.694 ± 0.016	8.378LeuSer: 8.378 ± 0.022	5.302LeuThr: 5.302 ± 0.016	5.62LeuVal: 5.62 ± 0.016	1.088LeuTrp: 1.088 ± 0.006	2.702LeuTyr: 2.702 ± 0.011	0.0LeuXaa: 0.0 ± 0.0
Met	1.877MetAla: 1.877 ± 0.008	0.504MetCys: 0.504 ± 0.005	1.462MetAsp: 1.462 ± 0.007	1.989MetGlu: 1.989 ± 0.009	0.91MetPhe: 0.91 ± 0.005	1.425MetGly: 1.425 ± 0.009	0.497MetHis: 0.497 ± 0.004	0.922MetIle: 0.922 ± 0.006	1.508MetLys: 1.508 ± 0.008	2.167MetLeu: 2.167 ± 0.009	0.747MetMet: 0.747 ± 0.02	0.912MetAsn: 0.912 ± 0.006	1.032MetPro: 1.032 ± 0.007	0.988MetGln: 0.988 ± 0.006	1.183MetArg: 1.183 ± 0.007	1.888MetSer: 1.888 ± 0.008	1.295MetThr: 1.295 ± 0.009	1.623MetVal: 1.623 ± 0.013	0.277MetTrp: 0.277 ± 0.004	0.657MetTyr: 0.657 ± 0.005	0.0MetXaa: 0.0 ± 0.0
Asn	2.206AsnAla: 2.206 ± 0.009	0.933AsnCys: 0.933 ± 0.007	1.743AsnAsp: 1.743 ± 0.01	2.079AsnGlu: 2.079 ± 0.009	1.473AsnPhe: 1.473 ± 0.007	2.8AsnGly: 2.8 ± 0.013	1.016AsnHis: 1.016 ± 0.006	2.258AsnIle: 2.258 ± 0.009	2.202AsnLys: 2.202 ± 0.008	3.74AsnLeu: 3.74 ± 0.012	1.059AsnMet: 1.059 ± 0.006	1.825AsnAsn: 1.825 ± 0.01	2.336AsnPro: 2.336 ± 0.011	1.79AsnGln: 1.79 ± 0.009	1.999AsnArg: 1.999 ± 0.009	3.207AsnSer: 3.207 ± 0.011	2.25AsnThr: 2.25 ± 0.01	2.361AsnVal: 2.361 ± 0.01	0.463AsnTrp: 0.463 ± 0.003	1.18AsnTyr: 1.18 ± 0.006	0.0AsnXaa: 0.0 ± 0.0
Pro	4.008ProAla: 4.008 ± 0.018	1.067ProCys: 1.067 ± 0.009	2.861ProAsp: 2.861 ± 0.013	3.67ProGlu: 3.67 ± 0.012	1.847ProPhe: 1.847 ± 0.009	4.057ProGly: 4.057 ± 0.038	1.533ProHis: 1.533 ± 0.01	1.945ProIle: 1.945 ± 0.01	2.45ProLys: 2.45 ± 0.016	4.905ProLeu: 4.905 ± 0.015	0.989ProMet: 0.989 ± 0.006	1.951ProAsn: 1.951 ± 0.009	5.355ProPro: 5.355 ± 0.037	2.593ProGln: 2.593 ± 0.015	2.715ProArg: 2.715 ± 0.012	5.495ProSer: 5.495 ± 0.028	3.049ProThr: 3.049 ± 0.019	3.775ProVal: 3.775 ± 0.021	0.568ProTrp: 0.568 ± 0.006	1.419ProTyr: 1.419 ± 0.008	0.0ProXaa: 0.0 ± 0.0
Gln	3.124GlnAla: 3.124 ± 0.015	0.971GlnCys: 0.971 ± 0.009	2.306GlnAsp: 2.306 ± 0.009	3.386GlnGlu: 3.386 ± 0.015	1.426GlnPhe: 1.426 ± 0.008	2.692GlnGly: 2.692 ± 0.013	1.392GlnHis: 1.392 ± 0.009	2.013GlnIle: 2.013 ± 0.01	2.618GlnLys: 2.618 ± 0.013	4.553GlnLeu: 4.553 ± 0.016	1.196GlnMet: 1.196 ± 0.007	1.81GlnAsn: 1.81 ± 0.008	2.465GlnPro: 2.465 ± 0.014	3.19GlnGln: 3.19 ± 0.026	3.025GlnArg: 3.025 ± 0.013	3.312GlnSer: 3.312 ± 0.012	2.466GlnThr: 2.466 ± 0.011	2.876GlnVal: 2.876 ± 0.012	0.547GlnTrp: 0.547 ± 0.005	1.268GlnTyr: 1.268 ± 0.007	0.0GlnXaa: 0.0 ± 0.0
Arg	3.435ArgAla: 3.435 ± 0.012	1.314ArgCys: 1.314 ± 0.011	2.953ArgAsp: 2.953 ± 0.011	3.805ArgGlu: 3.805 ± 0.017	2.041ArgPhe: 2.041 ± 0.008	3.498ArgGly: 3.498 ± 0.017	1.575ArgHis: 1.575 ± 0.008	2.477ArgIle: 2.477 ± 0.009	3.44ArgLys: 3.44 ± 0.014	5.383ArgLeu: 5.383 ± 0.018	1.323ArgMet: 1.323 ± 0.007	2.208ArgAsn: 2.208 ± 0.009	2.926ArgPro: 2.926 ± 0.014	2.667ArgGln: 2.667 ± 0.013	4.333ArgArg: 4.333 ± 0.022	4.493ArgSer: 4.493 ± 0.018	2.956ArgThr: 2.956 ± 0.012	3.373ArgVal: 3.373 ± 0.012	0.697ArgTrp: 0.697 ± 0.005	1.577ArgTyr: 1.577 ± 0.008	0.0ArgXaa: 0.0 ± 0.0
Ser	5.509SerAla: 5.509 ± 0.018	2.019SerCys: 2.019 ± 0.013	4.219SerAsp: 4.219 ± 0.014	4.78SerGlu: 4.78 ± 0.017	3.146SerPhe: 3.146 ± 0.012	5.594SerGly: 5.594 ± 0.02	2.276SerHis: 2.276 ± 0.011	3.506SerIle: 3.506 ± 0.017	3.891SerLys: 3.891 ± 0.012	8.322SerLeu: 8.322 ± 0.024	1.775SerMet: 1.775 ± 0.009	3.021SerAsn: 3.021 ± 0.012	5.733SerPro: 5.733 ± 0.026	3.748SerGln: 3.748 ± 0.014	4.548SerArg: 4.548 ± 0.017	9.678SerSer: 9.678 ± 0.04	4.675SerThr: 4.675 ± 0.018	5.439SerVal: 5.439 ± 0.023	1.026SerTrp: 1.026 ± 0.007	2.167SerTyr: 2.167 ± 0.01	0.0SerXaa: 0.0 ± 0.0
Thr	3.938ThrAla: 3.938 ± 0.013	1.389ThrCys: 1.389 ± 0.011	2.859ThrAsp: 2.859 ± 0.012	3.61ThrGlu: 3.61 ± 0.022	2.205ThrPhe: 2.205 ± 0.008	3.768ThrGly: 3.768 ± 0.023	1.482ThrHis: 1.482 ± 0.014	2.486ThrIle: 2.486 ± 0.01	2.598ThrLys: 2.598 ± 0.01	5.446ThrLeu: 5.446 ± 0.015	1.229ThrMet: 1.229 ± 0.009	1.938ThrAsn: 1.938 ± 0.01	3.473ThrPro: 3.473 ± 0.018	2.285ThrGln: 2.285 ± 0.011	2.531ThrArg: 2.531 ± 0.012	4.752ThrSer: 4.752 ± 0.022	3.241ThrThr: 3.241 ± 0.041	4.078ThrVal: 4.078 ± 0.016	0.67ThrTrp: 0.67 ± 0.006	1.464ThrTyr: 1.464 ± 0.008	0.0ThrXaa: 0.0 ± 0.0
Val	4.301ValAla: 4.301 ± 0.015	1.79ValCys: 1.79 ± 0.013	3.294ValAsp: 3.294 ± 0.013	3.955ValGlu: 3.955 ± 0.014	2.742ValPhe: 2.742 ± 0.011	3.648ValGly: 3.648 ± 0.013	1.665ValHis: 1.665 ± 0.009	3.102ValIle: 3.102 ± 0.012	3.547ValLys: 3.547 ± 0.012	6.368ValLeu: 6.368 ± 0.018	1.562ValMet: 1.562 ± 0.013	2.502ValAsn: 2.502 ± 0.01	3.323ValPro: 3.323 ± 0.014	2.753ValGln: 2.753 ± 0.012	3.374ValArg: 3.374 ± 0.012	5.296ValSer: 5.296 ± 0.016	4.002ValThr: 4.002 ± 0.024	4.504ValVal: 4.504 ± 0.024	0.815ValTrp: 0.815 ± 0.007	1.855ValTyr: 1.855 ± 0.01	0.0ValXaa: 0.0 ± 0.0
Trp	0.684TrpAla: 0.684 ± 0.005	0.274TrpCys: 0.274 ± 0.004	0.654TrpAsp: 0.654 ± 0.005	0.758TrpGlu: 0.758 ± 0.006	0.49TrpPhe: 0.49 ± 0.004	0.644TrpGly: 0.644 ± 0.006	0.285TrpHis: 0.285 ± 0.003	0.579TrpIle: 0.579 ± 0.004	0.745TrpLys: 0.745 ± 0.005	1.164TrpLeu: 1.164 ± 0.007	0.362TrpMet: 0.362 ± 0.004	0.553TrpAsn: 0.553 ± 0.005	0.441TrpPro: 0.441 ± 0.004	0.493TrpGln: 0.493 ± 0.004	0.748TrpArg: 0.748 ± 0.005	0.985TrpSer: 0.985 ± 0.007	0.733TrpThr: 0.733 ± 0.005	0.707TrpVal: 0.707 ± 0.005	0.212TrpTrp: 0.212 ± 0.003	0.342TrpTyr: 0.342 ± 0.003	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.474TyrAla: 1.474 ± 0.007	0.746TyrCys: 0.746 ± 0.005	1.405TyrAsp: 1.405 ± 0.007	1.645TyrGlu: 1.645 ± 0.006	1.241TyrPhe: 1.241 ± 0.007	1.736TyrGly: 1.736 ± 0.009	0.803TyrHis: 0.803 ± 0.006	1.548TyrIle: 1.548 ± 0.008	1.474TyrLys: 1.474 ± 0.007	2.736TyrLeu: 2.736 ± 0.01	0.698TyrMet: 0.698 ± 0.006	1.234TyrAsn: 1.234 ± 0.007	1.297TyrPro: 1.297 ± 0.008	1.271TyrGln: 1.271 ± 0.007	1.721TyrArg: 1.721 ± 0.009	2.361TyrSer: 2.361 ± 0.011	1.644TyrThr: 1.644 ± 0.008	1.64TyrVal: 1.64 ± 0.009	0.382TyrTrp: 0.382 ± 0.004	0.994TyrTyr: 0.994 ± 0.006	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 51089 proteins (32382901 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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