Moellerella wisconsensis ATCC 35017
Average proteome isoelectric point is 6.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2776 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0N1KJ43|A0A0N1KJ43_9GAMM Chaperone protein Skp OS=Moellerella wisconsensis ATCC 35017 OX=1354267 GN=M992_0046 PE=3 SV=1
MM1 pKa = 7.53 NFRR4 pKa = 11.84 GNLIGNPPCDD14 pKa = 2.97 ITGDD18 pKa = 3.76 NDD20 pKa = 5.1 PITVDD25 pKa = 3.94 FDD27 pKa = 3.83 EE28 pKa = 4.99 VGITKK33 pKa = 9.53 IDD35 pKa = 4.41 GINYY39 pKa = 7.39 MQDD42 pKa = 2.79 FALTVTCGNDD52 pKa = 3.12 LGNSVQLYY60 pKa = 8.78 MGYY63 pKa = 10.86 DD64 pKa = 3.53 GMNAPFDD71 pKa = 3.79 NDD73 pKa = 3.33 AVQTSLRR80 pKa = 11.84 GLGIRR85 pKa = 11.84 LYY87 pKa = 10.67 HH88 pKa = 5.85 QGQVVSPNNDD98 pKa = 4.38 DD99 pKa = 3.49 IPIIMSSGSSAIVPLSAVPVRR120 pKa = 11.84 DD121 pKa = 3.33 PAIDD125 pKa = 3.5 LFEE128 pKa = 5.78 GIFTATGTVEE138 pKa = 3.27 IRR140 pKa = 11.84 YY141 pKa = 8.68 PP142 pKa = 3.37
Molecular weight: 15.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.719
IPC2_protein 3.77
IPC_protein 3.757
Toseland 3.528
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.77
Rodwell 3.592
Grimsley 3.439
Solomon 3.77
Lehninger 3.719
Nozaki 3.91
DTASelect 4.215
Thurlkill 3.605
EMBOSS 3.77
Sillero 3.897
Patrickios 1.914
IPC_peptide 3.757
IPC2_peptide 3.859
IPC2.peptide.svr19 3.805
Protein with the highest isoelectric point:
>tr|A0A0N0Z9D5|A0A0N0Z9D5_9GAMM Thiol:disulfide interchange protein DsbD OS=Moellerella wisconsensis ATCC 35017 OX=1354267 GN=dsbD PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.53 RR3 pKa = 11.84 TFQPSILKK11 pKa = 10.25 RR12 pKa = 11.84 NRR14 pKa = 11.84 SHH16 pKa = 7.26 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MANKK25 pKa = 9.98 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.33 GRR39 pKa = 11.84 SRR41 pKa = 11.84 LTASSKK47 pKa = 11.0
Molecular weight: 5.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2776
0
2776
888818
37
3417
320.2
35.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.474 ± 0.053
1.034 ± 0.018
5.247 ± 0.037
5.705 ± 0.053
3.981 ± 0.035
6.812 ± 0.048
2.212 ± 0.021
7.39 ± 0.041
5.148 ± 0.038
10.608 ± 0.06
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.619 ± 0.025
4.567 ± 0.048
4.144 ± 0.034
4.929 ± 0.046
4.743 ± 0.041
6.373 ± 0.032
5.258 ± 0.033
6.316 ± 0.039
1.275 ± 0.021
3.164 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here