Candidimonas bauzanensis
Average proteome isoelectric point is 6.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5118 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1M5XCV2|A0A1M5XCV2_9BURK Uncharacterized protein OS=Candidimonas bauzanensis OX=658167 GN=SAMN04488135_106312 PE=4 SV=1
MM1 pKa = 7.85 DD2 pKa = 6.75 PILATLPPSLLALVEE17 pKa = 4.5 GSLSNDD23 pKa = 2.79 EE24 pKa = 4.32 VSSDD28 pKa = 4.01 EE29 pKa = 4.14 EE30 pKa = 3.84 MLAYY34 pKa = 10.24 FIDD37 pKa = 3.95 NGLTEE42 pKa = 4.41 DD43 pKa = 3.76 QARR46 pKa = 11.84 QALTYY51 pKa = 10.19 RR52 pKa = 11.84 DD53 pKa = 3.41 QYY55 pKa = 11.36 LNNIYY60 pKa = 10.76 LEE62 pKa = 4.48 GFTPITSADD71 pKa = 3.8 EE72 pKa = 4.11 PLHH75 pKa = 6.08 FNPHH79 pKa = 4.38 TRR81 pKa = 11.84 QFEE84 pKa = 3.94 PDD86 pKa = 2.92
Molecular weight: 9.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.761
IPC2_protein 3.859
IPC_protein 3.783
Toseland 3.592
ProMoST 3.859
Dawson 3.77
Bjellqvist 3.986
Wikipedia 3.694
Rodwell 3.617
Grimsley 3.503
Solomon 3.745
Lehninger 3.706
Nozaki 3.897
DTASelect 4.075
Thurlkill 3.656
EMBOSS 3.706
Sillero 3.897
Patrickios 1.875
IPC_peptide 3.745
IPC2_peptide 3.872
IPC2.peptide.svr19 3.846
Protein with the highest isoelectric point:
>tr|A0A1M5YQP0|A0A1M5YQP0_9BURK AraC-type DNA-binding protein OS=Candidimonas bauzanensis OX=658167 GN=SAMN04488135_11061 PE=4 SV=1
MM1 pKa = 7.21 LQTWLWARR9 pKa = 11.84 FKK11 pKa = 10.79 GVKK14 pKa = 8.89 RR15 pKa = 11.84 TVSRR19 pKa = 11.84 KK20 pKa = 6.22 TRR22 pKa = 11.84 QVHH25 pKa = 4.92 RR26 pKa = 11.84 LSRR29 pKa = 11.84 RR30 pKa = 11.84 TIQFSLVLVGAALVSFVSLGFAEE53 pKa = 5.04 LVDD56 pKa = 4.26 LALALNHH63 pKa = 6.74 RR64 pKa = 11.84 WLQAAPWAVWLILPFGLALLRR85 pKa = 11.84 WWTLRR90 pKa = 11.84 FAPNAGGSGIPQVIAALSLPADD112 pKa = 3.63 SGRR115 pKa = 11.84 RR116 pKa = 11.84 SLVALSQAAWKK127 pKa = 10.62 VPLTFLGLMCGASIGRR143 pKa = 11.84 EE144 pKa = 4.25 GPSVQVGAALMLAWGQACKK163 pKa = 10.69 ALGVPLKK170 pKa = 10.62 GIHH173 pKa = 5.21 TEE175 pKa = 3.91 EE176 pKa = 5.38 LIATGAAGGLAAAFNAPLAGVIFAIEE202 pKa = 3.75 EE203 pKa = 4.27 LGRR206 pKa = 11.84 GTALRR211 pKa = 11.84 WQRR214 pKa = 11.84 LVLIGVLAAGFIVVAFAGNNPYY236 pKa = 10.38 FGEE239 pKa = 4.3 FTGQALTHH247 pKa = 7.15 DD248 pKa = 3.98 MLGWTLLCGVACGVGGGLFSWLLSKK273 pKa = 10.79 GAASFAPSIWRR284 pKa = 11.84 GWIRR288 pKa = 11.84 RR289 pKa = 11.84 HH290 pKa = 5.2 PVWLAFIMGLIVAVLGTLTNNSVYY314 pKa = 9.44 GTGYY318 pKa = 10.64 GVASTALAGQQLEE331 pKa = 4.51 SAGFGPAKK339 pKa = 10.48 LLATVASYY347 pKa = 9.18 WAGIPGGIFTPALTTGAGIGAEE369 pKa = 4.07 LASWVGGVADD379 pKa = 3.78 QKK381 pKa = 11.59 VLVLLSMAAFLAAATQSPLTASVVVMEE408 pKa = 4.61 MTGSQPMLFWLLVCSLVASIVSRR431 pKa = 11.84 LFSPLPFYY439 pKa = 10.58 HH440 pKa = 6.82 HH441 pKa = 6.01 SAGRR445 pKa = 11.84 FRR447 pKa = 11.84 QQVRR451 pKa = 11.84 EE452 pKa = 3.95 VDD454 pKa = 3.33 RR455 pKa = 11.84 AQAKK459 pKa = 9.63
Molecular weight: 48.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.353
IPC2_protein 9.633
IPC_protein 10.467
Toseland 10.687
ProMoST 10.438
Dawson 10.774
Bjellqvist 10.496
Wikipedia 10.994
Rodwell 10.965
Grimsley 10.818
Solomon 10.906
Lehninger 10.862
Nozaki 10.687
DTASelect 10.496
Thurlkill 10.687
EMBOSS 11.082
Sillero 10.716
Patrickios 10.672
IPC_peptide 10.906
IPC2_peptide 9.677
IPC2.peptide.svr19 8.599
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5118
0
5118
1690431
39
5775
330.3
35.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.518 ± 0.047
0.922 ± 0.012
5.264 ± 0.027
5.036 ± 0.034
3.518 ± 0.022
8.523 ± 0.051
2.166 ± 0.018
5.158 ± 0.027
3.285 ± 0.027
10.784 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.52 ± 0.019
2.777 ± 0.027
5.104 ± 0.028
3.977 ± 0.021
6.676 ± 0.039
5.808 ± 0.033
4.853 ± 0.034
7.21 ± 0.028
1.392 ± 0.015
2.508 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here