Capybara microvirus Cap1_SP_209
Average proteome isoelectric point is 6.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W852|A0A4P8W852_9VIRU Minor capsid protein OS=Capybara microvirus Cap1_SP_209 OX=2585410 PE=4 SV=1
MM1 pKa = 6.78 TAFRR5 pKa = 11.84 SRR7 pKa = 11.84 FDD9 pKa = 3.58 YY10 pKa = 11.54 GEE12 pKa = 3.88 MPGTVNSEE20 pKa = 3.71 PSMTIQSEE28 pKa = 4.07 ADD30 pKa = 3.56 STSVEE35 pKa = 3.93 YY36 pKa = 10.75 ALRR39 pKa = 11.84 RR40 pKa = 11.84 FNATGILGDD49 pKa = 3.88 PDD51 pKa = 3.71 RR52 pKa = 11.84 LASAAYY58 pKa = 10.29 LDD60 pKa = 4.04 VSDD63 pKa = 4.39 VQDD66 pKa = 4.63 FEE68 pKa = 4.42 QSCNVVARR76 pKa = 11.84 ARR78 pKa = 11.84 QVFEE82 pKa = 4.36 ALPSEE87 pKa = 4.39 EE88 pKa = 3.59 RR89 pKa = 11.84 RR90 pKa = 11.84 KK91 pKa = 10.86 YY92 pKa = 10.54 GDD94 pKa = 3.3 SLQVYY99 pKa = 8.28 MEE101 pKa = 4.24 SRR103 pKa = 11.84 LEE105 pKa = 4.2 EE106 pKa = 3.89 IAQVSGAPSPVVEE119 pKa = 4.61 EE120 pKa = 4.75 TPSSAAPDD128 pKa = 3.33 AAAKK132 pKa = 10.32 VDD134 pKa = 3.48
Molecular weight: 14.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.425
IPC2_protein 4.317
IPC_protein 4.228
Toseland 4.05
ProMoST 4.279
Dawson 4.177
Bjellqvist 4.368
Wikipedia 4.05
Rodwell 4.05
Grimsley 3.961
Solomon 4.164
Lehninger 4.126
Nozaki 4.291
DTASelect 4.444
Thurlkill 4.062
EMBOSS 4.075
Sillero 4.329
Patrickios 3.821
IPC_peptide 4.177
IPC2_peptide 4.317
IPC2.peptide.svr19 4.271
Protein with the highest isoelectric point:
>tr|A0A4P8W5N3|A0A4P8W5N3_9VIRU Minor capsid protein OS=Capybara microvirus Cap1_SP_209 OX=2585410 PE=4 SV=1
MM1 pKa = 7.61 PCYY4 pKa = 10.32 HH5 pKa = 7.36 PVQAWRR11 pKa = 11.84 VRR13 pKa = 11.84 SGRR16 pKa = 11.84 NEE18 pKa = 3.72 NGKK21 pKa = 8.87 WPITFQFKK29 pKa = 10.02 QGYY32 pKa = 7.92 ADD34 pKa = 3.46 KK35 pKa = 10.24 EE36 pKa = 3.96 IRR38 pKa = 11.84 VPCGQCIGCRR48 pKa = 11.84 LEE50 pKa = 4.42 KK51 pKa = 10.69 SRR53 pKa = 11.84 VWALRR58 pKa = 11.84 CLHH61 pKa = 6.72 EE62 pKa = 4.64 SQLHH66 pKa = 4.66 EE67 pKa = 4.14 QCCFLTLTYY76 pKa = 10.18 RR77 pKa = 11.84 DD78 pKa = 3.74 NPGTLVLRR86 pKa = 11.84 DD87 pKa = 3.48 LQNFFKK93 pKa = 10.73 RR94 pKa = 11.84 LRR96 pKa = 11.84 KK97 pKa = 9.52 AIYY100 pKa = 8.09 PIKK103 pKa = 9.97 VRR105 pKa = 11.84 YY106 pKa = 7.28 YY107 pKa = 9.44 ACGEE111 pKa = 3.99 YY112 pKa = 10.56 GSNFGRR118 pKa = 11.84 PHH120 pKa = 4.66 YY121 pKa = 10.41 HH122 pKa = 6.81 CLLFGYY128 pKa = 9.97 DD129 pKa = 3.72 FSRR132 pKa = 11.84 DD133 pKa = 2.96 RR134 pKa = 11.84 VLFKK138 pKa = 10.89 RR139 pKa = 11.84 SGTNALYY146 pKa = 10.41 SSRR149 pKa = 11.84 LLDD152 pKa = 3.7 SLWTHH157 pKa = 6.86 GYY159 pKa = 10.34 CLIGDD164 pKa = 3.82 VSFDD168 pKa = 3.02 SCAYY172 pKa = 7.89 VARR175 pKa = 11.84 YY176 pKa = 8.43 CVKK179 pKa = 10.42 KK180 pKa = 9.35 VTGEE184 pKa = 4.21 GKK186 pKa = 9.7 VEE188 pKa = 4.36 HH189 pKa = 6.62 YY190 pKa = 10.8 VDD192 pKa = 4.84 PDD194 pKa = 3.29 TGEE197 pKa = 3.72 LRR199 pKa = 11.84 KK200 pKa = 10.0 PEE202 pKa = 4.01 FAVMSRR208 pKa = 11.84 RR209 pKa = 11.84 PGIGLDD215 pKa = 2.73 WFLRR219 pKa = 11.84 YY220 pKa = 9.12 RR221 pKa = 11.84 DD222 pKa = 3.22 EE223 pKa = 4.48 VMAHH227 pKa = 6.66 EE228 pKa = 4.96 GMCYY232 pKa = 9.92 INGVFCKK239 pKa = 10.1 IPRR242 pKa = 11.84 YY243 pKa = 9.69 YY244 pKa = 10.35 EE245 pKa = 4.14 EE246 pKa = 5.47 KK247 pKa = 10.46 DD248 pKa = 3.11 ACAGNDD254 pKa = 3.65 GLVKK258 pKa = 10.49 LRR260 pKa = 11.84 QARR263 pKa = 11.84 AAAARR268 pKa = 11.84 RR269 pKa = 11.84 VQDD272 pKa = 3.59 MGEE275 pKa = 3.89 QSPDD279 pKa = 2.88 RR280 pKa = 11.84 LAVRR284 pKa = 11.84 EE285 pKa = 4.19 EE286 pKa = 4.03 CAEE289 pKa = 4.09 MNLARR294 pKa = 11.84 LKK296 pKa = 10.63 RR297 pKa = 11.84 GYY299 pKa = 10.61 EE300 pKa = 3.85 EE301 pKa = 3.96 TASFLQPFEE310 pKa = 4.09
Molecular weight: 36.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.929
IPC2_protein 7.995
IPC_protein 7.79
Toseland 7.293
ProMoST 8.375
Dawson 8.492
Bjellqvist 8.916
Wikipedia 8.419
Rodwell 8.521
Grimsley 7.293
Solomon 8.551
Lehninger 8.565
Nozaki 9.107
DTASelect 8.595
Thurlkill 8.639
EMBOSS 8.682
Sillero 8.99
Patrickios 3.961
IPC_peptide 8.551
IPC2_peptide 8.156
IPC2.peptide.svr19 8.303
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1341
85
578
268.2
29.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.799 ± 1.944
1.342 ± 1.041
6.115 ± 0.547
4.847 ± 1.026
4.773 ± 0.446
7.383 ± 0.758
1.79 ± 0.362
3.505 ± 0.845
3.579 ± 0.581
8.352 ± 0.528
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.237 ± 0.67
4.549 ± 0.751
4.847 ± 0.819
4.773 ± 0.476
7.084 ± 0.947
8.949 ± 1.317
5.444 ± 1.178
6.04 ± 0.847
1.566 ± 0.332
4.027 ± 0.601
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here