Arthrobacter silviterrae
Average proteome isoelectric point is 6.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3795 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6M1QVK6|A0A6M1QVK6_9MICC NAD(P)H-binding protein OS=Arthrobacter silviterrae OX=2026658 GN=G6N77_12555 PE=4 SV=1
MM1 pKa = 7.73 SDD3 pKa = 2.82 SSTPAGVVVCQDD15 pKa = 2.62 SDD17 pKa = 4.55 VEE19 pKa = 4.75 VKK21 pKa = 10.74 SSLLVEE27 pKa = 4.44 VDD29 pKa = 4.33 DD30 pKa = 4.61 FPIAIVRR37 pKa = 11.84 DD38 pKa = 4.02 SYY40 pKa = 11.96 GVLHH44 pKa = 7.43 AIGDD48 pKa = 4.24 TCSHH52 pKa = 6.63 AEE54 pKa = 3.68 ISLSEE59 pKa = 4.11 GDD61 pKa = 4.18 VEE63 pKa = 6.36 DD64 pKa = 4.25 GTIEE68 pKa = 4.13 CWAHH72 pKa = 6.22 GSSFDD77 pKa = 4.37 LKK79 pKa = 10.01 TGEE82 pKa = 4.76 PLTLPAFEE90 pKa = 4.63 PVPVFNLEE98 pKa = 3.93 VHH100 pKa = 6.87 DD101 pKa = 4.36 GDD103 pKa = 4.62 VYY105 pKa = 11.77 VDD107 pKa = 3.17 VTNILNGVSIDD118 pKa = 3.55 NN119 pKa = 3.94
Molecular weight: 12.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.713
IPC2_protein 3.719
IPC_protein 3.694
Toseland 3.49
ProMoST 3.872
Dawson 3.694
Bjellqvist 3.859
Wikipedia 3.643
Rodwell 3.528
Grimsley 3.401
Solomon 3.681
Lehninger 3.643
Nozaki 3.821
DTASelect 4.05
Thurlkill 3.554
EMBOSS 3.656
Sillero 3.821
Patrickios 0.896
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.745
Protein with the highest isoelectric point:
>tr|A0A6M1QRS9|A0A6M1QRS9_9MICC Uncharacterized protein OS=Arthrobacter silviterrae OX=2026658 GN=G6N77_16915 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.42 KK7 pKa = 8.47 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.22 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.87 KK16 pKa = 9.7 HH17 pKa = 5.84 RR18 pKa = 11.84 KK19 pKa = 8.56 LLRR22 pKa = 11.84 KK23 pKa = 7.78 TRR25 pKa = 11.84 HH26 pKa = 3.65 QRR28 pKa = 11.84 RR29 pKa = 11.84 NKK31 pKa = 9.78 KK32 pKa = 9.85
Molecular weight: 4.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3795
0
3795
1248229
30
2046
328.9
35.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.617 ± 0.065
0.617 ± 0.01
5.352 ± 0.034
5.077 ± 0.043
3.205 ± 0.024
9.376 ± 0.038
2.15 ± 0.018
4.28 ± 0.031
2.804 ± 0.031
10.273 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.115 ± 0.016
2.58 ± 0.024
5.51 ± 0.033
3.126 ± 0.021
6.226 ± 0.043
5.799 ± 0.028
5.961 ± 0.039
8.391 ± 0.042
1.476 ± 0.015
2.066 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here