Listeria virus P61
Average proteome isoelectric point is 6.24
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 190 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6M9Z2T8|A0A6M9Z2T8_9CAUD DUF4379 domain-containing protein OS=Listeria virus P61 OX=2697238 PE=4 SV=1
MM1 pKa = 8.16 DD2 pKa = 4.63 SNKK5 pKa = 9.97 FLMGLNKK12 pKa = 9.86 FLTEE16 pKa = 3.74 IDD18 pKa = 4.21 KK19 pKa = 9.39 VTSAYY24 pKa = 10.55 GEE26 pKa = 4.07 DD27 pKa = 3.4 ANYY30 pKa = 9.69 EE31 pKa = 3.62 ISFIRR36 pKa = 11.84 EE37 pKa = 3.29 KK38 pKa = 11.03 CADD41 pKa = 3.99 LDD43 pKa = 3.87 EE44 pKa = 5.07 GAKK47 pKa = 9.62 QDD49 pKa = 4.56 CEE51 pKa = 3.97 EE52 pKa = 4.06 TDD54 pKa = 3.78 FFEE57 pKa = 4.34 GAEE60 pKa = 4.68 YY61 pKa = 9.94 ISQMQTGEE69 pKa = 4.24 DD70 pKa = 3.72 CFEE73 pKa = 4.16 GYY75 pKa = 9.85 ILRR78 pKa = 11.84 KK79 pKa = 9.45 IKK81 pKa = 9.58 NTDD84 pKa = 2.4 WCLCITYY91 pKa = 10.4 AII93 pKa = 4.41
Molecular weight: 10.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.883
IPC2_protein 4.164
IPC_protein 4.075
Toseland 3.897
ProMoST 4.215
Dawson 4.037
Bjellqvist 4.19
Wikipedia 3.935
Rodwell 3.91
Grimsley 3.808
Solomon 4.037
Lehninger 3.986
Nozaki 4.151
DTASelect 4.317
Thurlkill 3.923
EMBOSS 3.948
Sillero 4.19
Patrickios 1.939
IPC_peptide 4.037
IPC2_peptide 4.177
IPC2.peptide.svr19 4.088
Protein with the highest isoelectric point:
>tr|A0A6M9Z2Y9|A0A6M9Z2Y9_9CAUD Uncharacterized protein OS=Listeria virus P61 OX=2697238 PE=4 SV=1
MM1 pKa = 7.54 KK2 pKa = 10.29 KK3 pKa = 10.63 LSRR6 pKa = 11.84 DD7 pKa = 3.59 EE8 pKa = 3.73 IKK10 pKa = 10.47 KK11 pKa = 8.84 RR12 pKa = 11.84 RR13 pKa = 11.84 KK14 pKa = 9.99 YY15 pKa = 10.55 FINEE19 pKa = 4.07 AKK21 pKa = 10.23 RR22 pKa = 11.84 VTHH25 pKa = 6.21 IRR27 pKa = 11.84 AGDD30 pKa = 3.4 RR31 pKa = 11.84 RR32 pKa = 11.84 IATGSRR38 pKa = 11.84 ICTLCGRR45 pKa = 11.84 SLSSQKK51 pKa = 10.43 RR52 pKa = 11.84 STGEE56 pKa = 3.6 TFASVNHH63 pKa = 4.94 IHH65 pKa = 7.42 CYY67 pKa = 9.89 FNEE70 pKa = 3.87 LLIVNLCEE78 pKa = 5.26 DD79 pKa = 3.22 ATSCYY84 pKa = 9.65 RR85 pKa = 11.84 RR86 pKa = 11.84 HH87 pKa = 5.75 EE88 pKa = 4.21 RR89 pKa = 11.84 SS90 pKa = 3.0
Molecular weight: 10.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.276
IPC2_protein 9.311
IPC_protein 9.502
Toseland 10.189
ProMoST 9.838
Dawson 10.335
Bjellqvist 10.043
Wikipedia 10.496
Rodwell 10.672
Grimsley 10.394
Solomon 10.394
Lehninger 10.379
Nozaki 10.277
DTASelect 9.999
Thurlkill 10.218
EMBOSS 10.584
Sillero 10.277
Patrickios 10.423
IPC_peptide 10.394
IPC2_peptide 9.238
IPC2.peptide.svr19 8.19
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
190
0
190
39911
38
1313
210.1
23.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.733 ± 0.223
0.639 ± 0.06
5.941 ± 0.117
8.261 ± 0.275
3.924 ± 0.124
5.896 ± 0.271
1.501 ± 0.097
6.63 ± 0.207
8.609 ± 0.272
8.514 ± 0.203
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.438 ± 0.085
5.628 ± 0.132
2.841 ± 0.127
3.393 ± 0.197
4.122 ± 0.134
6.795 ± 0.173
6.299 ± 0.174
7.326 ± 0.186
0.967 ± 0.055
4.477 ± 0.138
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here