Ralstonia virus RSA1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Aresaunavirus

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A4PE44|A4PE44_9CAUD Phage tail protein gpI OS=Ralstonia virus RSA1 OX=406340 PE=4 SV=1
MM1 pKa = 7.76AIIDD5 pKa = 4.58LSQLPAPAVVEE16 pKa = 4.17TLDD19 pKa = 3.58YY20 pKa = 10.97EE21 pKa = 5.09AILAEE26 pKa = 4.14RR27 pKa = 11.84KK28 pKa = 10.06DD29 pKa = 4.22YY30 pKa = 10.62FVSLHH35 pKa = 6.49PADD38 pKa = 3.73QRR40 pKa = 11.84DD41 pKa = 3.47AARR44 pKa = 11.84ATLALEE50 pKa = 4.3SEE52 pKa = 5.3PITKK56 pKa = 10.36LLQEE60 pKa = 3.83NAYY63 pKa = 10.37RR64 pKa = 11.84EE65 pKa = 4.4LVWRR69 pKa = 11.84QRR71 pKa = 11.84VNDD74 pKa = 3.86AARR77 pKa = 11.84GVMLAFAEE85 pKa = 4.78GEE87 pKa = 4.05DD88 pKa = 4.37LEE90 pKa = 5.07QIAANFNVQRR100 pKa = 11.84LTITPADD107 pKa = 3.96TTTVPPTPAVMEE119 pKa = 4.7GDD121 pKa = 3.37DD122 pKa = 4.08SLRR125 pKa = 11.84EE126 pKa = 3.81RR127 pKa = 11.84AQEE130 pKa = 3.88AFEE133 pKa = 4.36GLSVAGPTKK142 pKa = 10.29AYY144 pKa = 9.83EE145 pKa = 4.0QFARR149 pKa = 11.84SADD152 pKa = 3.31GRR154 pKa = 11.84VADD157 pKa = 4.86ARR159 pKa = 11.84AISPAGAEE167 pKa = 4.17VVVSVLSHH175 pKa = 6.64LNDD178 pKa = 3.59GAADD182 pKa = 3.93EE183 pKa = 4.69SLLAAVRR190 pKa = 11.84TALSDD195 pKa = 3.83DD196 pKa = 3.66DD197 pKa = 4.2TRR199 pKa = 11.84PLGDD203 pKa = 3.4RR204 pKa = 11.84LTVQSATIVPYY215 pKa = 10.48RR216 pKa = 11.84IRR218 pKa = 11.84ATLYY222 pKa = 10.2LASGPAAEE230 pKa = 5.8PILDD234 pKa = 3.94AAGKK238 pKa = 9.8RR239 pKa = 11.84ADD241 pKa = 3.33VYY243 pKa = 9.03RR244 pKa = 11.84TTRR247 pKa = 11.84RR248 pKa = 11.84RR249 pKa = 11.84IGRR252 pKa = 11.84DD253 pKa = 2.83INRR256 pKa = 11.84SAITAALHH264 pKa = 5.37VEE266 pKa = 4.34GVEE269 pKa = 3.92KK270 pKa = 10.8LVLHH274 pKa = 6.68EE275 pKa = 4.3PAEE278 pKa = 4.85DD279 pKa = 3.24IALDD283 pKa = 3.93LTQAGYY289 pKa = 8.4CTGVDD294 pKa = 3.11IVNGGTSEE302 pKa = 3.94

Molecular weight: 32.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A4PE69|A4PE69_9CAUD Conserved hypothetical phage protein OS=Ralstonia virus RSA1 OX=406340 PE=4 SV=1
MM1 pKa = 7.61SILVLPLRR9 pKa = 11.84GDD11 pKa = 3.79FFDD14 pKa = 5.21QIPAGTKK21 pKa = 8.86CEE23 pKa = 4.08EE24 pKa = 3.81YY25 pKa = 10.45RR26 pKa = 11.84LCKK29 pKa = 9.08PHH31 pKa = 5.76WRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84LEE37 pKa = 3.65GRR39 pKa = 11.84TFDD42 pKa = 5.0RR43 pKa = 11.84IEE45 pKa = 3.9LTRR48 pKa = 11.84GYY50 pKa = 9.8PRR52 pKa = 11.84RR53 pKa = 11.84DD54 pKa = 3.1DD55 pKa = 3.48SARR58 pKa = 11.84RR59 pKa = 11.84LPRR62 pKa = 11.84PWRR65 pKa = 11.84GYY67 pKa = 8.85IIKK70 pKa = 9.51TITHH74 pKa = 5.9PHH76 pKa = 6.84FGPEE80 pKa = 3.95PVQVFAIRR88 pKa = 11.84VNVTPEE94 pKa = 3.73TMM96 pKa = 3.55

Molecular weight: 11.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

12635

54

934

247.7

27.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.083 ± 0.576

1.14 ± 0.128

5.691 ± 0.286

5.089 ± 0.229

3.031 ± 0.221

8.112 ± 0.383

2.295 ± 0.196

3.997 ± 0.209

3.712 ± 0.325

9.102 ± 0.331

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.082 ± 0.163

2.928 ± 0.246

4.812 ± 0.245

3.918 ± 0.2

8.223 ± 0.405

5.018 ± 0.291

5.912 ± 0.484

7.661 ± 0.372

1.67 ± 0.168

2.525 ± 0.173
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski