Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales; Methanocaldococcaceae; Methanotorris; Methanotorris igneus

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1753 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F6BEK1|F6BEK1_METIK CGGC domain-containing protein OS=Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5) OX=880724 GN=Metig_0101 PE=4 SV=1
MM1 pKa = 7.53KK2 pKa = 9.93VTINYY7 pKa = 7.65DD8 pKa = 3.34LCKK11 pKa = 10.58GPEE14 pKa = 4.02CAEE17 pKa = 4.34CVNACPMEE25 pKa = 4.13VFEE28 pKa = 4.35IQGDD32 pKa = 4.03KK33 pKa = 10.96VVVARR38 pKa = 11.84EE39 pKa = 4.22EE40 pKa = 4.28DD41 pKa = 3.65CSGCGVCMDD50 pKa = 4.15VCPTNAVKK58 pKa = 10.93VEE60 pKa = 4.1DD61 pKa = 3.71

Molecular weight: 6.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F6BCE5|F6BCE5_METIK Appr-1-p processing domain protein OS=Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5) OX=880724 GN=Metig_0602 PE=4 SV=1
MM1 pKa = 7.82ASNKK5 pKa = 9.65PLGKK9 pKa = 10.09KK10 pKa = 9.78LRR12 pKa = 11.84LAKK15 pKa = 10.28ALKK18 pKa = 9.26QNRR21 pKa = 11.84RR22 pKa = 11.84VPLFVILKK30 pKa = 7.38TKK32 pKa = 10.3GRR34 pKa = 11.84VRR36 pKa = 11.84SHH38 pKa = 6.16PKK40 pKa = 7.89MRR42 pKa = 11.84FWRR45 pKa = 11.84RR46 pKa = 11.84SKK48 pKa = 11.03LKK50 pKa = 10.62AA51 pKa = 3.37

Molecular weight: 6.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1753

0

1753

501396

34

2719

286.0

32.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.409 ± 0.074

1.417 ± 0.032

5.5 ± 0.041

8.596 ± 0.069

4.066 ± 0.044

6.519 ± 0.051

1.574 ± 0.023

10.195 ± 0.057

10.247 ± 0.081

9.163 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.339 ± 0.03

5.345 ± 0.079

3.498 ± 0.034

1.457 ± 0.023

3.838 ± 0.041

4.599 ± 0.046

4.292 ± 0.04

7.089 ± 0.052

0.71 ± 0.02

4.146 ± 0.049
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski