Amino acid dipepetide frequency for Propionibacterium phage E6

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.64AlaAla: 18.64 ± 2.36
0.504AlaCys: 0.504 ± 0.214
7.976AlaAsp: 7.976 ± 0.962
6.213AlaGlu: 6.213 ± 0.895
3.023AlaPhe: 3.023 ± 0.684
10.495AlaGly: 10.495 ± 0.96
1.931AlaHis: 1.931 ± 0.39
6.129AlaIle: 6.129 ± 1.067
5.877AlaLys: 5.877 ± 0.783
11.167AlaLeu: 11.167 ± 1.439
3.61AlaMet: 3.61 ± 0.501
2.939AlaAsn: 2.939 ± 0.491
6.213AlaPro: 6.213 ± 0.624
5.458AlaGln: 5.458 ± 0.683
8.9AlaArg: 8.9 ± 1.623
8.228AlaSer: 8.228 ± 0.932
7.725AlaThr: 7.725 ± 0.708
7.557AlaVal: 7.557 ± 0.859
3.61AlaTrp: 3.61 ± 0.627
1.763AlaTyr: 1.763 ± 0.409
0.0AlaXaa: 0.0 ± 0.0
Cys
1.008CysAla: 1.008 ± 0.34
0.084CysCys: 0.084 ± 0.08
0.588CysAsp: 0.588 ± 0.219
0.336CysGlu: 0.336 ± 0.169
0.336CysPhe: 0.336 ± 0.219
1.008CysGly: 1.008 ± 0.329
0.084CysHis: 0.084 ± 0.089
0.42CysIle: 0.42 ± 0.197
0.336CysLys: 0.336 ± 0.191
0.42CysLeu: 0.42 ± 0.165
0.084CysMet: 0.084 ± 0.086
0.252CysAsn: 0.252 ± 0.152
0.756CysPro: 0.756 ± 0.26
0.336CysGln: 0.336 ± 0.183
1.092CysArg: 1.092 ± 0.358
0.756CysSer: 0.756 ± 0.242
0.672CysThr: 0.672 ± 0.257
0.252CysVal: 0.252 ± 0.138
0.168CysTrp: 0.168 ± 0.098
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
7.641AspAla: 7.641 ± 0.88
0.504AspCys: 0.504 ± 0.188
3.694AspAsp: 3.694 ± 0.714
4.366AspGlu: 4.366 ± 0.684
1.679AspPhe: 1.679 ± 0.437
7.305AspGly: 7.305 ± 0.826
0.924AspHis: 0.924 ± 0.302
2.183AspIle: 2.183 ± 0.432
1.847AspLys: 1.847 ± 0.341
5.709AspLeu: 5.709 ± 0.772
1.175AspMet: 1.175 ± 0.295
0.588AspAsn: 0.588 ± 0.161
4.198AspPro: 4.198 ± 0.743
2.183AspGln: 2.183 ± 0.406
4.954AspArg: 4.954 ± 0.639
2.687AspSer: 2.687 ± 0.629
3.442AspThr: 3.442 ± 0.692
4.45AspVal: 4.45 ± 0.694
1.343AspTrp: 1.343 ± 0.359
1.511AspTyr: 1.511 ± 0.413
0.0AspXaa: 0.0 ± 0.0
Glu
7.809GluAla: 7.809 ± 0.901
0.42GluCys: 0.42 ± 0.186
2.939GluAsp: 2.939 ± 0.458
2.603GluGlu: 2.603 ± 0.612
0.756GluPhe: 0.756 ± 0.205
2.771GluGly: 2.771 ± 0.664
1.259GluHis: 1.259 ± 0.236
2.267GluIle: 2.267 ± 0.303
1.427GluLys: 1.427 ± 0.494
6.381GluLeu: 6.381 ± 0.978
0.756GluMet: 0.756 ± 0.224
0.504GluAsn: 0.504 ± 0.149
3.442GluPro: 3.442 ± 0.846
2.519GluGln: 2.519 ± 0.406
3.526GluArg: 3.526 ± 0.698
3.275GluSer: 3.275 ± 0.536
3.526GluThr: 3.526 ± 0.632
3.359GluVal: 3.359 ± 0.554
1.847GluTrp: 1.847 ± 0.32
0.504GluTyr: 0.504 ± 0.197
0.0GluXaa: 0.0 ± 0.0
Phe
2.771PheAla: 2.771 ± 0.597
0.168PheCys: 0.168 ± 0.123
1.679PheAsp: 1.679 ± 0.369
1.008PheGlu: 1.008 ± 0.276
0.504PhePhe: 0.504 ± 0.201
3.191PheGly: 3.191 ± 0.8
0.588PheHis: 0.588 ± 0.214
1.092PheIle: 1.092 ± 0.306
1.259PheLys: 1.259 ± 0.297
1.931PheLeu: 1.931 ± 0.421
0.84PheMet: 0.84 ± 0.278
0.42PheAsn: 0.42 ± 0.315
1.259PhePro: 1.259 ± 0.414
1.679PheGln: 1.679 ± 0.286
1.427PheArg: 1.427 ± 0.251
1.175PheSer: 1.175 ± 0.479
2.351PheThr: 2.351 ± 0.358
1.763PheVal: 1.763 ± 0.386
0.672PheTrp: 0.672 ± 0.323
0.504PheTyr: 0.504 ± 0.182
0.0PheXaa: 0.0 ± 0.0
Gly
10.831GlyAla: 10.831 ± 1.463
0.756GlyCys: 0.756 ± 0.296
4.702GlyAsp: 4.702 ± 0.646
4.534GlyGlu: 4.534 ± 0.483
2.687GlyPhe: 2.687 ± 0.603
7.893GlyGly: 7.893 ± 1.212
2.099GlyHis: 2.099 ± 0.585
4.03GlyIle: 4.03 ± 0.739
2.855GlyLys: 2.855 ± 0.563
8.144GlyLeu: 8.144 ± 1.034
1.847GlyMet: 1.847 ± 0.457
1.763GlyAsn: 1.763 ± 0.333
4.03GlyPro: 4.03 ± 0.666
2.603GlyGln: 2.603 ± 0.992
5.793GlyArg: 5.793 ± 0.712
4.786GlySer: 4.786 ± 1.011
4.45GlyThr: 4.45 ± 0.489
6.717GlyVal: 6.717 ± 0.877
2.855GlyTrp: 2.855 ± 0.419
1.595GlyTyr: 1.595 ± 0.321
0.0GlyXaa: 0.0 ± 0.0
His
1.595HisAla: 1.595 ± 0.391
0.252HisCys: 0.252 ± 0.145
0.924HisAsp: 0.924 ± 0.251
1.427HisGlu: 1.427 ± 0.394
0.672HisPhe: 0.672 ± 0.25
1.175HisGly: 1.175 ± 0.294
0.504HisHis: 0.504 ± 0.229
0.756HisIle: 0.756 ± 0.275
0.588HisLys: 0.588 ± 0.281
1.427HisLeu: 1.427 ± 0.404
0.504HisMet: 0.504 ± 0.191
0.42HisAsn: 0.42 ± 0.317
1.092HisPro: 1.092 ± 0.353
1.008HisGln: 1.008 ± 0.294
1.175HisArg: 1.175 ± 0.403
0.924HisSer: 0.924 ± 0.276
1.343HisThr: 1.343 ± 0.413
1.259HisVal: 1.259 ± 0.3
0.588HisTrp: 0.588 ± 0.221
0.504HisTyr: 0.504 ± 0.268
0.0HisXaa: 0.0 ± 0.0
Ile
4.954IleAla: 4.954 ± 0.818
0.336IleCys: 0.336 ± 0.167
4.45IleAsp: 4.45 ± 0.641
3.275IleGlu: 3.275 ± 0.452
1.343IlePhe: 1.343 ± 0.396
4.03IleGly: 4.03 ± 1.477
0.84IleHis: 0.84 ± 0.279
2.351IleIle: 2.351 ± 0.63
1.343IleLys: 1.343 ± 0.279
3.107IleLeu: 3.107 ± 0.795
0.756IleMet: 0.756 ± 0.228
0.84IleAsn: 0.84 ± 0.243
3.023IlePro: 3.023 ± 0.431
2.015IleGln: 2.015 ± 0.454
2.519IleArg: 2.519 ± 0.503
2.687IleSer: 2.687 ± 0.782
3.778IleThr: 3.778 ± 0.547
3.359IleVal: 3.359 ± 0.728
0.672IleTrp: 0.672 ± 0.176
0.924IleTyr: 0.924 ± 0.256
0.0IleXaa: 0.0 ± 0.0
Lys
5.122LysAla: 5.122 ± 0.914
0.252LysCys: 0.252 ± 0.156
1.595LysAsp: 1.595 ± 0.423
1.175LysGlu: 1.175 ± 0.232
1.008LysPhe: 1.008 ± 0.384
2.519LysGly: 2.519 ± 0.552
0.672LysHis: 0.672 ± 0.328
1.259LysIle: 1.259 ± 0.409
1.175LysLys: 1.175 ± 0.303
2.351LysLeu: 2.351 ± 0.427
0.924LysMet: 0.924 ± 0.248
0.672LysAsn: 0.672 ± 0.206
2.267LysPro: 2.267 ± 0.395
2.099LysGln: 2.099 ± 0.65
4.03LysArg: 4.03 ± 0.733
1.847LysSer: 1.847 ± 0.292
2.603LysThr: 2.603 ± 0.594
2.771LysVal: 2.771 ± 0.52
0.42LysTrp: 0.42 ± 0.264
0.504LysTyr: 0.504 ± 0.239
0.0LysXaa: 0.0 ± 0.0
Leu
11.419LeuAla: 11.419 ± 1.285
0.756LeuCys: 0.756 ± 0.251
5.542LeuAsp: 5.542 ± 0.609
3.442LeuGlu: 3.442 ± 0.672
1.847LeuPhe: 1.847 ± 0.37
7.893LeuGly: 7.893 ± 1.117
1.092LeuHis: 1.092 ± 0.347
3.778LeuIle: 3.778 ± 1.089
2.939LeuLys: 2.939 ± 0.408
5.961LeuLeu: 5.961 ± 0.977
2.015LeuMet: 2.015 ± 0.458
1.511LeuAsn: 1.511 ± 0.447
5.458LeuPro: 5.458 ± 0.985
2.519LeuGln: 2.519 ± 0.599
6.129LeuArg: 6.129 ± 0.939
6.885LeuSer: 6.885 ± 1.002
6.549LeuThr: 6.549 ± 0.848
5.542LeuVal: 5.542 ± 0.984
1.931LeuTrp: 1.931 ± 0.407
0.504LeuTyr: 0.504 ± 0.207
0.0LeuXaa: 0.0 ± 0.0
Met
2.435MetAla: 2.435 ± 0.483
0.168MetCys: 0.168 ± 0.136
1.092MetAsp: 1.092 ± 0.272
1.008MetGlu: 1.008 ± 0.321
0.336MetPhe: 0.336 ± 0.168
1.511MetGly: 1.511 ± 0.465
0.42MetHis: 0.42 ± 0.17
1.175MetIle: 1.175 ± 0.255
1.092MetLys: 1.092 ± 0.309
1.931MetLeu: 1.931 ± 0.475
0.588MetMet: 0.588 ± 0.23
0.756MetAsn: 0.756 ± 0.223
1.343MetPro: 1.343 ± 0.373
1.008MetGln: 1.008 ± 0.238
1.763MetArg: 1.763 ± 0.467
2.687MetSer: 2.687 ± 0.389
1.847MetThr: 1.847 ± 0.293
1.343MetVal: 1.343 ± 0.34
0.504MetTrp: 0.504 ± 0.184
0.168MetTyr: 0.168 ± 0.106
0.0MetXaa: 0.0 ± 0.0
Asn
3.023AsnAla: 3.023 ± 0.541
0.168AsnCys: 0.168 ± 0.114
1.092AsnAsp: 1.092 ± 0.37
0.42AsnGlu: 0.42 ± 0.208
0.336AsnPhe: 0.336 ± 0.158
2.351AsnGly: 2.351 ± 0.446
0.336AsnHis: 0.336 ± 0.142
1.175AsnIle: 1.175 ± 0.261
0.252AsnLys: 0.252 ± 0.167
1.343AsnLeu: 1.343 ± 0.393
0.42AsnMet: 0.42 ± 0.252
0.336AsnAsn: 0.336 ± 0.133
1.931AsnPro: 1.931 ± 0.424
0.756AsnGln: 0.756 ± 0.285
2.015AsnArg: 2.015 ± 0.467
1.008AsnSer: 1.008 ± 0.285
0.84AsnThr: 0.84 ± 0.214
2.015AsnVal: 2.015 ± 0.342
0.336AsnTrp: 0.336 ± 0.313
0.336AsnTyr: 0.336 ± 0.163
0.0AsnXaa: 0.0 ± 0.0
Pro
7.389ProAla: 7.389 ± 0.973
0.336ProCys: 0.336 ± 0.182
4.618ProAsp: 4.618 ± 0.919
3.442ProGlu: 3.442 ± 0.451
2.099ProPhe: 2.099 ± 0.434
4.03ProGly: 4.03 ± 0.677
1.175ProHis: 1.175 ± 0.268
2.351ProIle: 2.351 ± 0.417
2.183ProLys: 2.183 ± 0.494
3.359ProLeu: 3.359 ± 0.632
1.259ProMet: 1.259 ± 0.327
1.343ProAsn: 1.343 ± 0.428
3.191ProPro: 3.191 ± 0.852
1.679ProGln: 1.679 ± 0.435
2.771ProArg: 2.771 ± 0.618
4.45ProSer: 4.45 ± 0.737
3.61ProThr: 3.61 ± 0.707
4.702ProVal: 4.702 ± 1.083
1.175ProTrp: 1.175 ± 0.323
0.924ProTyr: 0.924 ± 0.478
0.0ProXaa: 0.0 ± 0.0
Gln
5.458GlnAla: 5.458 ± 0.557
0.336GlnCys: 0.336 ± 0.176
1.595GlnAsp: 1.595 ± 0.325
1.763GlnGlu: 1.763 ± 0.412
1.259GlnPhe: 1.259 ± 0.363
2.603GlnGly: 2.603 ± 0.451
0.42GlnHis: 0.42 ± 0.211
2.603GlnIle: 2.603 ± 0.565
1.092GlnLys: 1.092 ± 0.245
4.114GlnLeu: 4.114 ± 1.026
0.84GlnMet: 0.84 ± 0.356
0.42GlnAsn: 0.42 ± 0.179
1.847GlnPro: 1.847 ± 0.356
2.183GlnGln: 2.183 ± 0.398
3.107GlnArg: 3.107 ± 0.639
1.595GlnSer: 1.595 ± 0.346
2.351GlnThr: 2.351 ± 0.646
4.03GlnVal: 4.03 ± 0.724
1.175GlnTrp: 1.175 ± 0.309
0.504GlnTyr: 0.504 ± 0.261
0.0GlnXaa: 0.0 ± 0.0
Arg
7.641ArgAla: 7.641 ± 0.9
1.259ArgCys: 1.259 ± 0.321
4.786ArgAsp: 4.786 ± 0.772
4.45ArgGlu: 4.45 ± 0.621
1.679ArgPhe: 1.679 ± 0.359
4.786ArgGly: 4.786 ± 0.772
1.763ArgHis: 1.763 ± 0.419
2.939ArgIle: 2.939 ± 0.631
2.855ArgLys: 2.855 ± 0.604
5.709ArgLeu: 5.709 ± 0.83
1.679ArgMet: 1.679 ± 0.453
2.267ArgAsn: 2.267 ± 0.458
3.946ArgPro: 3.946 ± 0.787
2.267ArgGln: 2.267 ± 0.457
6.045ArgArg: 6.045 ± 1.074
3.946ArgSer: 3.946 ± 0.618
4.87ArgThr: 4.87 ± 0.806
4.702ArgVal: 4.702 ± 0.75
2.351ArgTrp: 2.351 ± 0.553
1.175ArgTyr: 1.175 ± 0.338
0.0ArgXaa: 0.0 ± 0.0
Ser
7.725SerAla: 7.725 ± 1.091
0.504SerCys: 0.504 ± 0.244
4.366SerAsp: 4.366 ± 0.707
3.191SerGlu: 3.191 ± 0.598
1.931SerPhe: 1.931 ± 0.449
5.206SerGly: 5.206 ± 0.756
1.175SerHis: 1.175 ± 0.346
2.687SerIle: 2.687 ± 0.601
2.099SerLys: 2.099 ± 0.444
5.206SerLeu: 5.206 ± 0.724
1.931SerMet: 1.931 ± 0.443
1.343SerAsn: 1.343 ± 0.317
2.519SerPro: 2.519 ± 0.406
2.099SerGln: 2.099 ± 0.485
4.618SerArg: 4.618 ± 0.539
3.862SerSer: 3.862 ± 0.765
4.87SerThr: 4.87 ± 0.695
5.206SerVal: 5.206 ± 0.645
1.763SerTrp: 1.763 ± 0.438
0.924SerTyr: 0.924 ± 0.319
0.0SerXaa: 0.0 ± 0.0
Thr
6.633ThrAla: 6.633 ± 0.653
1.175ThrCys: 1.175 ± 0.452
4.534ThrAsp: 4.534 ± 0.959
2.435ThrGlu: 2.435 ± 0.533
1.595ThrPhe: 1.595 ± 0.418
6.129ThrGly: 6.129 ± 0.767
1.008ThrHis: 1.008 ± 0.359
4.198ThrIle: 4.198 ± 0.612
2.687ThrLys: 2.687 ± 0.518
6.381ThrLeu: 6.381 ± 0.787
1.259ThrMet: 1.259 ± 0.308
1.595ThrAsn: 1.595 ± 0.356
4.198ThrPro: 4.198 ± 0.559
2.099ThrGln: 2.099 ± 0.467
3.275ThrArg: 3.275 ± 0.576
4.534ThrSer: 4.534 ± 0.664
5.793ThrThr: 5.793 ± 0.888
5.206ThrVal: 5.206 ± 0.554
1.595ThrTrp: 1.595 ± 0.397
0.84ThrTyr: 0.84 ± 0.272
0.0ThrXaa: 0.0 ± 0.0
Val
10.915ValAla: 10.915 ± 1.082
0.756ValCys: 0.756 ± 0.256
3.442ValAsp: 3.442 ± 0.488
4.954ValGlu: 4.954 ± 0.771
1.931ValPhe: 1.931 ± 0.59
6.465ValGly: 6.465 ± 1.384
0.84ValHis: 0.84 ± 0.262
3.61ValIle: 3.61 ± 0.747
1.931ValLys: 1.931 ± 0.351
6.045ValLeu: 6.045 ± 0.88
1.847ValMet: 1.847 ± 0.36
1.511ValAsn: 1.511 ± 0.329
3.359ValPro: 3.359 ± 0.557
3.023ValGln: 3.023 ± 0.435
4.702ValArg: 4.702 ± 0.568
5.374ValSer: 5.374 ± 0.721
4.198ValThr: 4.198 ± 0.626
5.038ValVal: 5.038 ± 0.896
2.099ValTrp: 2.099 ± 0.579
0.84ValTyr: 0.84 ± 0.239
0.0ValXaa: 0.0 ± 0.0
Trp
2.771TrpAla: 2.771 ± 0.482
0.084TrpCys: 0.084 ± 0.096
2.267TrpAsp: 2.267 ± 0.359
1.427TrpGlu: 1.427 ± 0.424
0.42TrpPhe: 0.42 ± 0.162
1.931TrpGly: 1.931 ± 0.546
0.756TrpHis: 0.756 ± 0.272
1.343TrpIle: 1.343 ± 0.341
0.84TrpLys: 0.84 ± 0.23
2.015TrpLeu: 2.015 ± 0.364
0.672TrpMet: 0.672 ± 0.236
0.588TrpAsn: 0.588 ± 0.212
1.511TrpPro: 1.511 ± 0.38
0.756TrpGln: 0.756 ± 0.26
2.519TrpArg: 2.519 ± 0.63
1.511TrpSer: 1.511 ± 0.487
1.259TrpThr: 1.259 ± 0.326
2.435TrpVal: 2.435 ± 0.418
0.42TrpTrp: 0.42 ± 0.191
0.336TrpTyr: 0.336 ± 0.152
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.183TyrAla: 2.183 ± 0.473
0.168TyrCys: 0.168 ± 0.139
0.504TyrAsp: 0.504 ± 0.185
0.42TyrGlu: 0.42 ± 0.187
0.84TyrPhe: 0.84 ± 0.262
1.679TyrGly: 1.679 ± 0.431
0.252TyrHis: 0.252 ± 0.166
0.252TyrIle: 0.252 ± 0.127
0.588TyrLys: 0.588 ± 0.206
1.008TyrLeu: 1.008 ± 0.314
0.084TyrMet: 0.084 ± 0.089
0.42TyrAsn: 0.42 ± 0.195
0.504TyrPro: 0.504 ± 0.337
1.008TyrGln: 1.008 ± 0.271
0.756TyrArg: 0.756 ± 0.221
1.092TyrSer: 1.092 ± 0.326
1.092TyrThr: 1.092 ± 0.249
1.175TyrVal: 1.175 ± 0.223
0.336TyrTrp: 0.336 ± 0.175
0.168TyrTyr: 0.168 ± 0.125
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 60 proteins (11911 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski