Eilatimonas milleporae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Kordiimonadales; Kordiimonadaceae; Eilatimonas

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3747 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3M0C5R8|A0A3M0C5R8_9PROT Dimethylaniline monooxygenase (N-oxide forming) OS=Eilatimonas milleporae OX=911205 GN=BXY39_2840 PE=3 SV=1
MM1 pKa = 7.63TGTSAQRR8 pKa = 11.84LVLYY12 pKa = 7.33TQNADD17 pKa = 3.67RR18 pKa = 11.84LSDD21 pKa = 3.79VVSTPITDD29 pKa = 4.93FIVTFIINTSEE40 pKa = 4.2DD41 pKa = 3.33PMTFTFSGGDD51 pKa = 3.39VSALKK56 pKa = 10.84SEE58 pKa = 4.44VEE60 pKa = 4.14AVQAAGKK67 pKa = 9.96RR68 pKa = 11.84VLISFGGGTMGSPNYY83 pKa = 9.71QPLVGHH89 pKa = 5.96EE90 pKa = 4.12TAVADD95 pKa = 3.65ALTRR99 pKa = 11.84FVAEE103 pKa = 4.76GGFDD107 pKa = 5.32GIDD110 pKa = 3.73LDD112 pKa = 4.9YY113 pKa = 11.3EE114 pKa = 4.35DD115 pKa = 5.0TGALAYY121 pKa = 10.1GDD123 pKa = 4.75PYY125 pKa = 11.39DD126 pKa = 3.94GVAFISDD133 pKa = 4.13LTNALAAAFQSTFPGQKK150 pKa = 9.94KK151 pKa = 10.45YY152 pKa = 8.78ITHH155 pKa = 7.28APQGPYY161 pKa = 9.73FVQGWNFAYY170 pKa = 10.25QQVLANTGDD179 pKa = 4.1AIDD182 pKa = 3.76YY183 pKa = 11.0LNIQFYY189 pKa = 10.78NNPGFEE195 pKa = 4.3GPSDD199 pKa = 3.45ILSCYY204 pKa = 9.13TDD206 pKa = 3.03WADD209 pKa = 3.71GANGQTPIDD218 pKa = 4.15PSRR221 pKa = 11.84MVIGLPVTAGDD232 pKa = 3.84AGSGYY237 pKa = 10.54VPLDD241 pKa = 3.46QIPPQIIAPVSKK253 pKa = 10.66AFPTFAGMMGWQFLSDD269 pKa = 4.05PQDD272 pKa = 2.96TWVDD276 pKa = 3.73TIGAALHH283 pKa = 5.87LQSATRR289 pKa = 11.84AAEE292 pKa = 3.77

Molecular weight: 31.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3M0C9L4|A0A3M0C9L4_9PROT Uncharacterized protein OS=Eilatimonas milleporae OX=911205 GN=BXY39_2619 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVIVRR12 pKa = 11.84KK13 pKa = 9.41RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.55GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVAGRR28 pKa = 11.84KK29 pKa = 8.81ILNARR34 pKa = 11.84RR35 pKa = 11.84GKK37 pKa = 9.77GRR39 pKa = 11.84KK40 pKa = 8.97RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3747

0

3747

1277968

30

3164

341.1

37.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.883 ± 0.056

0.815 ± 0.013

6.897 ± 0.044

5.282 ± 0.039

3.803 ± 0.029

8.9 ± 0.043

2.119 ± 0.02

4.957 ± 0.026

2.96 ± 0.034

9.914 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.455 ± 0.021

2.686 ± 0.034

5.098 ± 0.029

3.02 ± 0.023

7.262 ± 0.046

5.303 ± 0.03

5.899 ± 0.039

7.173 ± 0.032

1.259 ± 0.014

2.315 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski