Chryseotalea sanaruensis
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3948 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A401UCJ0|A0A401UCJ0_9BACT Damage-inducible protein DinB OS=Chryseotalea sanaruensis OX=2482724 GN=SanaruYs_28400 PE=3 SV=1
MM1 pKa = 8.17 RR2 pKa = 11.84 DD3 pKa = 3.12 NEE5 pKa = 4.63 LIITNKK11 pKa = 9.37 MIKK14 pKa = 9.76 KK15 pKa = 9.87 NLMKK19 pKa = 10.32 HH20 pKa = 5.5 KK21 pKa = 9.42 RR22 pKa = 11.84 NSYY25 pKa = 10.37 IPFLLLSSIGLLLSCDD41 pKa = 3.79 NEE43 pKa = 4.66 DD44 pKa = 3.47 EE45 pKa = 4.48 VTTSFEE51 pKa = 4.51 RR52 pKa = 11.84 VSSTYY57 pKa = 10.88 EE58 pKa = 3.91 EE59 pKa = 4.25 INGTGEE65 pKa = 3.92 VVIPLRR71 pKa = 11.84 STGSITSDD79 pKa = 2.63 NVTVQFDD86 pKa = 3.6 GTATEE91 pKa = 4.92 GEE93 pKa = 4.27 DD94 pKa = 4.15 FEE96 pKa = 5.26 LVSINSEE103 pKa = 4.07 SMVIRR108 pKa = 11.84 VIDD111 pKa = 4.11 DD112 pKa = 3.49 NLFEE116 pKa = 4.07 EE117 pKa = 4.71 TEE119 pKa = 4.28 YY120 pKa = 11.36 VRR122 pKa = 11.84 VLLVSNGANVGGNAIHH138 pKa = 7.23 RR139 pKa = 11.84 LNIVSNCEE147 pKa = 4.12 DD148 pKa = 3.06 IGGLDD153 pKa = 3.17 VGFFAGDD160 pKa = 3.25 YY161 pKa = 9.98 DD162 pKa = 3.99 ALEE165 pKa = 4.95 DD166 pKa = 3.87 YY167 pKa = 11.33 GGGTTYY173 pKa = 10.98 GPYY176 pKa = 10.25 TITLVQDD183 pKa = 3.57 EE184 pKa = 4.62 EE185 pKa = 4.84 DD186 pKa = 3.43 PTRR189 pKa = 11.84 FSFDD193 pKa = 3.05 NLYY196 pKa = 11.14 DD197 pKa = 4.02 SGCDD201 pKa = 2.78 AYY203 pKa = 10.92 MIFDD207 pKa = 3.94 YY208 pKa = 11.55 ANGTVAFPNQSPCDD222 pKa = 3.84 SPLTNSSGTFSIDD235 pKa = 2.99 LCEE238 pKa = 4.13 EE239 pKa = 3.86 TTLTINLNFDD249 pKa = 3.37 GGDD252 pKa = 2.74 WVYY255 pKa = 11.33 RR256 pKa = 11.84 FTKK259 pKa = 10.55 LL260 pKa = 3.86
Molecular weight: 28.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.767
IPC2_protein 3.935
IPC_protein 3.923
Toseland 3.719
ProMoST 4.062
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.808
Rodwell 3.745
Grimsley 3.617
Solomon 3.897
Lehninger 3.846
Nozaki 4.012
DTASelect 4.215
Thurlkill 3.757
EMBOSS 3.821
Sillero 4.037
Patrickios 1.901
IPC_peptide 3.897
IPC2_peptide 4.024
IPC2.peptide.svr19 3.936
Protein with the highest isoelectric point:
>tr|A0A401UA79|A0A401UA79_9BACT Efflux transporter periplasmic adaptor subunit OS=Chryseotalea sanaruensis OX=2482724 GN=SanaruYs_20160 PE=4 SV=1
MM1 pKa = 7.5 ARR3 pKa = 11.84 NDD5 pKa = 2.8 IRR7 pKa = 11.84 LRR9 pKa = 11.84 RR10 pKa = 11.84 QRR12 pKa = 11.84 MTTGNIARR20 pKa = 11.84 HH21 pKa = 5.57 RR22 pKa = 11.84 NYY24 pKa = 9.99 GDD26 pKa = 2.56 IMEE29 pKa = 4.26 RR30 pKa = 11.84 HH31 pKa = 6.09 EE32 pKa = 6.12 RR33 pKa = 11.84 DD34 pKa = 3.68 QKK36 pKa = 7.95 WKK38 pKa = 10.89 RR39 pKa = 11.84 MFKK42 pKa = 10.16 VFIYY46 pKa = 9.5 FLIAAFLTIILIIVLRR62 pKa = 11.84 WEE64 pKa = 3.71 QRR66 pKa = 11.84 EE67 pKa = 4.12 NKK69 pKa = 9.82 DD70 pKa = 4.29 LNANTKK76 pKa = 10.02 KK77 pKa = 10.57 AIEE80 pKa = 4.12 LASPPMLSS88 pKa = 3.11
Molecular weight: 10.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.313
IPC2_protein 9.794
IPC_protein 10.847
Toseland 11.067
ProMoST 11.067
Dawson 11.111
Bjellqvist 10.906
Wikipedia 11.403
Rodwell 11.213
Grimsley 11.14
Solomon 11.374
Lehninger 11.316
Nozaki 11.038
DTASelect 10.906
Thurlkill 11.052
EMBOSS 11.491
Sillero 11.067
Patrickios 10.965
IPC_peptide 11.374
IPC2_peptide 9.984
IPC2.peptide.svr19 8.747
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3948
0
3948
1390093
42
5619
352.1
39.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.104 ± 0.042
0.726 ± 0.013
5.263 ± 0.028
6.288 ± 0.042
5.047 ± 0.031
6.544 ± 0.045
1.793 ± 0.02
7.455 ± 0.031
6.992 ± 0.064
9.772 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.23 ± 0.021
5.539 ± 0.044
3.525 ± 0.023
3.756 ± 0.026
3.978 ± 0.026
6.689 ± 0.036
5.811 ± 0.057
6.502 ± 0.036
1.121 ± 0.016
3.864 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here