Sinimarinibacterium sp. CAU 1509
Average proteome isoelectric point is 6.5
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4153 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4U0GVY4|A0A4U0GVY4_9GAMM OmpW family protein OS=Sinimarinibacterium sp. CAU 1509 OX=2562283 GN=E4T66_05870 PE=4 SV=1
MM1 pKa = 7.47 KK2 pKa = 10.56 NSLLLLSATLLLCACGGGDD21 pKa = 3.89 SKK23 pKa = 11.38 LHH25 pKa = 6.0 EE26 pKa = 4.88 SRR28 pKa = 11.84 LSLSVTDD35 pKa = 4.77 APVDD39 pKa = 3.4 HH40 pKa = 7.4 ADD42 pKa = 3.32 AVVVSFISAEE52 pKa = 3.98 LLDD55 pKa = 4.02 ASGAVKK61 pKa = 10.3 EE62 pKa = 4.7 SFVLDD67 pKa = 3.76 PPLQIDD73 pKa = 4.26 LLSLQGANSAFLIDD87 pKa = 4.41 DD88 pKa = 3.79 AVVAPGVYY96 pKa = 9.44 QEE98 pKa = 4.35 VRR100 pKa = 11.84 LKK102 pKa = 10.88 VDD104 pKa = 4.17 AEE106 pKa = 4.15 NANCNAVSPPYY117 pKa = 10.51 ASYY120 pKa = 9.41 VTVDD124 pKa = 3.28 GVDD127 pKa = 3.41 YY128 pKa = 10.66 PLIVPSGGSSGFKK141 pKa = 10.41 VKK143 pKa = 10.68 GPLTVAVGGSVAYY156 pKa = 9.32 TIDD159 pKa = 3.3 FDD161 pKa = 4.23 LRR163 pKa = 11.84 QSIAEE168 pKa = 4.23 RR169 pKa = 11.84 GATDD173 pKa = 4.5 CYY175 pKa = 11.08 NLKK178 pKa = 10.25 PVLRR182 pKa = 11.84 VVDD185 pKa = 3.77 NATVGTLSGIIDD197 pKa = 4.18 GSLLADD203 pKa = 4.08 SSCTADD209 pKa = 3.16 TATGAGAAVYY219 pKa = 9.15 LFAGAGVTPDD229 pKa = 4.76 DD230 pKa = 4.19 VDD232 pKa = 3.51 GTTPEE237 pKa = 4.48 PLTSALLTATGNDD250 pKa = 3.12 QFSYY254 pKa = 10.96 EE255 pKa = 4.27 VGFLPAGAYY264 pKa = 7.82 TVALTCQAGDD274 pKa = 4.75 DD275 pKa = 4.41 DD276 pKa = 5.85 PEE278 pKa = 5.21 TDD280 pKa = 4.9 DD281 pKa = 5.6 PITFLRR287 pKa = 11.84 SVDD290 pKa = 3.65 VQVDD294 pKa = 3.43 ADD296 pKa = 4.04 SVTTQDD302 pKa = 3.14 FTAPSLVVQGSS313 pKa = 3.27
Molecular weight: 32.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.672
IPC2_protein 3.694
IPC_protein 3.732
Toseland 3.49
ProMoST 3.923
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.719
Rodwell 3.554
Grimsley 3.401
Solomon 3.745
Lehninger 3.694
Nozaki 3.859
DTASelect 4.164
Thurlkill 3.567
EMBOSS 3.719
Sillero 3.859
Patrickios 1.189
IPC_peptide 3.732
IPC2_peptide 3.834
IPC2.peptide.svr19 3.773
Protein with the highest isoelectric point:
>tr|A0A4U0GHX7|A0A4U0GHX7_9GAMM Nitrate reductase OS=Sinimarinibacterium sp. CAU 1509 OX=2562283 GN=E4T66_15880 PE=3 SV=1
MM1 pKa = 7.65 RR2 pKa = 11.84 RR3 pKa = 11.84 RR4 pKa = 11.84 PRR6 pKa = 11.84 RR7 pKa = 11.84 AWARR11 pKa = 11.84 SRR13 pKa = 11.84 APSPKK18 pKa = 9.1 PKK20 pKa = 9.82 PVLRR24 pKa = 11.84 RR25 pKa = 11.84 CRR27 pKa = 11.84 RR28 pKa = 11.84 RR29 pKa = 11.84 SHH31 pKa = 6.68 RR32 pKa = 11.84 CASWRR37 pKa = 11.84 RR38 pKa = 11.84 APPKK42 pKa = 9.74 RR43 pKa = 11.84 RR44 pKa = 11.84 RR45 pKa = 11.84 RR46 pKa = 11.84 PPRR49 pKa = 11.84 AWPRR53 pKa = 11.84 SKK55 pKa = 10.65 RR56 pKa = 11.84 RR57 pKa = 11.84 LKK59 pKa = 10.55 RR60 pKa = 11.84 SRR62 pKa = 11.84 QTMRR66 pKa = 11.84 RR67 pKa = 11.84 SQSGSTTTT75 pKa = 3.62
Molecular weight: 9.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.505
IPC2_protein 11.14
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.91
Bjellqvist 12.91
Wikipedia 13.378
Rodwell 12.486
Grimsley 12.939
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.193
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.164
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4153
0
4153
1371815
18
2984
330.3
36.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.13 ± 0.051
1.039 ± 0.013
5.836 ± 0.03
5.365 ± 0.034
3.439 ± 0.023
8.123 ± 0.033
2.265 ± 0.016
4.63 ± 0.027
2.794 ± 0.031
10.628 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.312 ± 0.017
2.611 ± 0.022
5.265 ± 0.033
3.965 ± 0.023
7.281 ± 0.04
5.707 ± 0.029
5.214 ± 0.028
7.425 ± 0.031
1.459 ± 0.016
2.513 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here