Sinimarinibacterium sp. CAU 1509

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Nevskiales; Sinobacteraceae; Sinimarinibacterium; unclassified Sinimarinibacterium

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4153 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4U0GVY4|A0A4U0GVY4_9GAMM OmpW family protein OS=Sinimarinibacterium sp. CAU 1509 OX=2562283 GN=E4T66_05870 PE=4 SV=1
MM1 pKa = 7.47KK2 pKa = 10.56NSLLLLSATLLLCACGGGDD21 pKa = 3.89SKK23 pKa = 11.38LHH25 pKa = 6.0EE26 pKa = 4.88SRR28 pKa = 11.84LSLSVTDD35 pKa = 4.77APVDD39 pKa = 3.4HH40 pKa = 7.4ADD42 pKa = 3.32AVVVSFISAEE52 pKa = 3.98LLDD55 pKa = 4.02ASGAVKK61 pKa = 10.3EE62 pKa = 4.7SFVLDD67 pKa = 3.76PPLQIDD73 pKa = 4.26LLSLQGANSAFLIDD87 pKa = 4.41DD88 pKa = 3.79AVVAPGVYY96 pKa = 9.44QEE98 pKa = 4.35VRR100 pKa = 11.84LKK102 pKa = 10.88VDD104 pKa = 4.17AEE106 pKa = 4.15NANCNAVSPPYY117 pKa = 10.51ASYY120 pKa = 9.41VTVDD124 pKa = 3.28GVDD127 pKa = 3.41YY128 pKa = 10.66PLIVPSGGSSGFKK141 pKa = 10.41VKK143 pKa = 10.68GPLTVAVGGSVAYY156 pKa = 9.32TIDD159 pKa = 3.3FDD161 pKa = 4.23LRR163 pKa = 11.84QSIAEE168 pKa = 4.23RR169 pKa = 11.84GATDD173 pKa = 4.5CYY175 pKa = 11.08NLKK178 pKa = 10.25PVLRR182 pKa = 11.84VVDD185 pKa = 3.77NATVGTLSGIIDD197 pKa = 4.18GSLLADD203 pKa = 4.08SSCTADD209 pKa = 3.16TATGAGAAVYY219 pKa = 9.15LFAGAGVTPDD229 pKa = 4.76DD230 pKa = 4.19VDD232 pKa = 3.51GTTPEE237 pKa = 4.48PLTSALLTATGNDD250 pKa = 3.12QFSYY254 pKa = 10.96EE255 pKa = 4.27VGFLPAGAYY264 pKa = 7.82TVALTCQAGDD274 pKa = 4.75DD275 pKa = 4.41DD276 pKa = 5.85PEE278 pKa = 5.21TDD280 pKa = 4.9DD281 pKa = 5.6PITFLRR287 pKa = 11.84SVDD290 pKa = 3.65VQVDD294 pKa = 3.43ADD296 pKa = 4.04SVTTQDD302 pKa = 3.14FTAPSLVVQGSS313 pKa = 3.27

Molecular weight:
32.05 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4U0GHX7|A0A4U0GHX7_9GAMM Nitrate reductase OS=Sinimarinibacterium sp. CAU 1509 OX=2562283 GN=E4T66_15880 PE=3 SV=1
MM1 pKa = 7.65RR2 pKa = 11.84RR3 pKa = 11.84RR4 pKa = 11.84PRR6 pKa = 11.84RR7 pKa = 11.84AWARR11 pKa = 11.84SRR13 pKa = 11.84APSPKK18 pKa = 9.1PKK20 pKa = 9.82PVLRR24 pKa = 11.84RR25 pKa = 11.84CRR27 pKa = 11.84RR28 pKa = 11.84RR29 pKa = 11.84SHH31 pKa = 6.68RR32 pKa = 11.84CASWRR37 pKa = 11.84RR38 pKa = 11.84APPKK42 pKa = 9.74RR43 pKa = 11.84RR44 pKa = 11.84RR45 pKa = 11.84RR46 pKa = 11.84PPRR49 pKa = 11.84AWPRR53 pKa = 11.84SKK55 pKa = 10.65RR56 pKa = 11.84RR57 pKa = 11.84LKK59 pKa = 10.55RR60 pKa = 11.84SRR62 pKa = 11.84QTMRR66 pKa = 11.84RR67 pKa = 11.84SQSGSTTTT75 pKa = 3.62

Molecular weight:
9.26 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4153

0

4153

1371815

18

2984

330.3

36.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.13 ± 0.051

1.039 ± 0.013

5.836 ± 0.03

5.365 ± 0.034

3.439 ± 0.023

8.123 ± 0.033

2.265 ± 0.016

4.63 ± 0.027

2.794 ± 0.031

10.628 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.312 ± 0.017

2.611 ± 0.022

5.265 ± 0.033

3.965 ± 0.023

7.281 ± 0.04

5.707 ± 0.029

5.214 ± 0.028

7.425 ± 0.031

1.459 ± 0.016

2.513 ± 0.022

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski