SAR202 cluster bacterium AD-804-J14_MRT_500m

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Chloroflexi; Chloroflexi incertae sedis; SAR202 cluster

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1639 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5N8YVC9|A0A5N8YVC9_9CHLR Aminopeptidase P family protein OS=SAR202 cluster bacterium AD-804-J14_MRT_500m OX=2587836 GN=FIM12_04530 PE=4 SV=1
MM1 pKa = 7.33YY2 pKa = 10.28IEE4 pKa = 4.78VDD6 pKa = 3.61VQEE9 pKa = 4.96GQNSLIPVNKK19 pKa = 9.39IFMAFDD25 pKa = 3.37SPTIVSHH32 pKa = 6.77VMAPDD37 pKa = 3.22SLGNEE42 pKa = 4.27RR43 pKa = 11.84WCTVTGWSASGPSPAYY59 pKa = 8.7TVLVEE64 pKa = 4.42DD65 pKa = 3.73SGEE68 pKa = 4.25GVVTLVYY75 pKa = 10.8GSDD78 pKa = 2.86QGIRR82 pKa = 11.84LKK84 pKa = 11.0DD85 pKa = 3.64ADD87 pKa = 5.7SEE89 pKa = 4.92DD90 pKa = 3.93PWDD93 pKa = 4.96LDD95 pKa = 4.67NPNQWGEE102 pKa = 3.91ACLLLDD108 pKa = 5.38QYY110 pKa = 8.1VTWAA114 pKa = 3.74

Molecular weight: 12.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5N8YTR4|A0A5N8YTR4_9CHLR 3-oxoacid CoA-transferase subunit A OS=SAR202 cluster bacterium AD-804-J14_MRT_500m OX=2587836 GN=FIM12_01455 PE=4 SV=1
MM1 pKa = 8.03PKK3 pKa = 8.99RR4 pKa = 11.84TYY6 pKa = 9.41QPKK9 pKa = 8.1NRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84KK14 pKa = 8.73RR15 pKa = 11.84VHH17 pKa = 5.6GFMRR21 pKa = 11.84RR22 pKa = 11.84MLSRR26 pKa = 11.84GGRR29 pKa = 11.84AVLKK33 pKa = 10.29NRR35 pKa = 11.84RR36 pKa = 11.84FKK38 pKa = 10.83GRR40 pKa = 11.84HH41 pKa = 4.67RR42 pKa = 11.84LAVV45 pKa = 3.37

Molecular weight: 5.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1639

0

1639

512167

26

1600

312.5

34.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.067 ± 0.058

1.016 ± 0.02

5.771 ± 0.046

6.023 ± 0.051

3.731 ± 0.042

8.395 ± 0.047

2.304 ± 0.027

6.611 ± 0.043

3.959 ± 0.035

9.678 ± 0.069

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.657 ± 0.028

3.616 ± 0.03

4.943 ± 0.043

3.428 ± 0.032

5.803 ± 0.043

6.819 ± 0.05

5.571 ± 0.052

7.488 ± 0.047

1.322 ± 0.024

2.797 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski