Mycobacterium phage BTCU-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9R4K3|R9R4K3_9CAUD Portal protein OS=Mycobacterium phage BTCU-1 OX=1262532 GN=CKC_10 PE=4 SV=1
MM1 pKa = 7.58AVTLQFIDD9 pKa = 4.3PNHH12 pKa = 6.79PSATIEE18 pKa = 4.29LDD20 pKa = 3.7LSDD23 pKa = 5.74FPDD26 pKa = 3.79ASTEE30 pKa = 3.83AGQEE34 pKa = 4.07VIEE37 pKa = 4.17AAIAALNPSPPGVYY51 pKa = 8.61VTEE54 pKa = 4.54VIYY57 pKa = 8.84DD58 pKa = 3.8TVEE61 pKa = 4.0VGEE64 pKa = 4.1DD65 pKa = 3.39TYY67 pKa = 11.02PYY69 pKa = 10.66KK70 pKa = 10.03RR71 pKa = 11.84TRR73 pKa = 11.84FYY75 pKa = 10.86YY76 pKa = 9.89TLQDD80 pKa = 3.74YY81 pKa = 10.64FDD83 pKa = 4.55SLSSS87 pKa = 3.38

Molecular weight: 9.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R9R4I8|R9R4I8_9CAUD Uncharacterized protein OS=Mycobacterium phage BTCU-1 OX=1262532 GN=CKC_39 PE=4 SV=1
MM1 pKa = 6.94IVARR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84VALGVASAGTVAVGGLAFALSFTALRR33 pKa = 11.84DD34 pKa = 3.56LSASNGVAQAWMVPLVVDD52 pKa = 3.79GGIIVATAATVALRR66 pKa = 11.84RR67 pKa = 11.84HH68 pKa = 5.22QWYY71 pKa = 9.42AWTLLILSSLVSVAGNVAHH90 pKa = 6.57AQAHH94 pKa = 5.49GAIAMVIAAIPPLWLLAATHH114 pKa = 5.73LTVMLSRR121 pKa = 11.84SDD123 pKa = 3.85KK124 pKa = 10.87EE125 pKa = 4.24PVPVAAEE132 pKa = 3.9PLHH135 pKa = 6.32IANAAA140 pKa = 3.29

Molecular weight: 14.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

73

0

73

13588

25

1014

186.1

20.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.707 ± 0.457

0.699 ± 0.1

6.197 ± 0.254

6.727 ± 0.379

3.407 ± 0.192

8.39 ± 0.691

1.943 ± 0.181

4.762 ± 0.158

4.129 ± 0.212

8.169 ± 0.313

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.451 ± 0.146

3.606 ± 0.246

5.858 ± 0.439

3.996 ± 0.39

6.579 ± 0.337

5.358 ± 0.228

5.924 ± 0.229

7.234 ± 0.26

1.891 ± 0.171

2.973 ± 0.215
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski