Amino acid dipepetide frequency for Mycobacterium phage BTCU-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.04AlaAla: 11.04 ± 1.165
0.662AlaCys: 0.662 ± 0.205
5.078AlaAsp: 5.078 ± 0.598
6.55AlaGlu: 6.55 ± 0.85
2.87AlaPhe: 2.87 ± 0.544
7.507AlaGly: 7.507 ± 0.803
1.914AlaHis: 1.914 ± 0.37
3.533AlaIle: 3.533 ± 0.522
4.637AlaLys: 4.637 ± 0.616
9.568AlaLeu: 9.568 ± 0.876
2.429AlaMet: 2.429 ± 0.424
2.797AlaAsn: 2.797 ± 0.37
5.078AlaPro: 5.078 ± 0.899
4.122AlaGln: 4.122 ± 0.471
5.741AlaArg: 5.741 ± 0.619
5.888AlaSer: 5.888 ± 0.683
6.035AlaThr: 6.035 ± 0.604
7.213AlaVal: 7.213 ± 0.806
2.355AlaTrp: 2.355 ± 0.376
2.061AlaTyr: 2.061 ± 0.355
0.0AlaXaa: 0.0 ± 0.0
Cys
0.515CysAla: 0.515 ± 0.185
0.0CysCys: 0.0 ± 0.0
0.294CysAsp: 0.294 ± 0.131
0.662CysGlu: 0.662 ± 0.214
0.221CysPhe: 0.221 ± 0.112
0.589CysGly: 0.589 ± 0.177
0.147CysHis: 0.147 ± 0.087
0.294CysIle: 0.294 ± 0.145
0.294CysLys: 0.294 ± 0.132
0.442CysLeu: 0.442 ± 0.197
0.074CysMet: 0.074 ± 0.072
0.515CysAsn: 0.515 ± 0.165
0.294CysPro: 0.294 ± 0.155
0.294CysGln: 0.294 ± 0.147
0.883CysArg: 0.883 ± 0.28
0.368CysSer: 0.368 ± 0.189
0.221CysThr: 0.221 ± 0.167
0.368CysVal: 0.368 ± 0.144
0.074CysTrp: 0.074 ± 0.062
0.442CysTyr: 0.442 ± 0.187
0.0CysXaa: 0.0 ± 0.0
Asp
5.667AspAla: 5.667 ± 0.614
0.589AspCys: 0.589 ± 0.201
3.901AspAsp: 3.901 ± 0.569
4.49AspGlu: 4.49 ± 0.563
2.65AspPhe: 2.65 ± 0.406
5.373AspGly: 5.373 ± 0.551
1.398AspHis: 1.398 ± 0.358
2.797AspIle: 2.797 ± 0.415
1.84AspLys: 1.84 ± 0.458
5.52AspLeu: 5.52 ± 0.567
0.957AspMet: 0.957 ± 0.248
2.061AspAsn: 2.061 ± 0.388
4.342AspPro: 4.342 ± 0.617
1.987AspGln: 1.987 ± 0.377
3.606AspArg: 3.606 ± 0.581
3.018AspSer: 3.018 ± 0.43
4.269AspThr: 4.269 ± 0.543
3.974AspVal: 3.974 ± 0.477
1.251AspTrp: 1.251 ± 0.227
2.208AspTyr: 2.208 ± 0.318
0.0AspXaa: 0.0 ± 0.0
Glu
6.845GluAla: 6.845 ± 0.822
0.515GluCys: 0.515 ± 0.22
4.416GluAsp: 4.416 ± 0.653
4.122GluGlu: 4.122 ± 0.542
2.797GluPhe: 2.797 ± 0.419
5.152GluGly: 5.152 ± 0.713
1.251GluHis: 1.251 ± 0.34
2.65GluIle: 2.65 ± 0.464
2.429GluLys: 2.429 ± 0.411
6.477GluLeu: 6.477 ± 0.914
1.766GluMet: 1.766 ± 0.347
2.208GluAsn: 2.208 ± 0.346
3.018GluPro: 3.018 ± 0.524
2.797GluGln: 2.797 ± 0.461
3.68GluArg: 3.68 ± 0.616
3.974GluSer: 3.974 ± 0.552
3.901GluThr: 3.901 ± 0.552
5.741GluVal: 5.741 ± 0.79
1.03GluTrp: 1.03 ± 0.246
2.502GluTyr: 2.502 ± 0.483
0.0GluXaa: 0.0 ± 0.0
Phe
3.68PheAla: 3.68 ± 0.581
0.147PheCys: 0.147 ± 0.094
3.312PheAsp: 3.312 ± 0.512
2.576PheGlu: 2.576 ± 0.429
0.442PhePhe: 0.442 ± 0.202
3.091PheGly: 3.091 ± 0.494
0.662PheHis: 0.662 ± 0.242
1.619PheIle: 1.619 ± 0.305
0.957PheLys: 0.957 ± 0.298
2.944PheLeu: 2.944 ± 0.512
0.662PheMet: 0.662 ± 0.245
1.987PheAsn: 1.987 ± 0.486
1.619PhePro: 1.619 ± 0.319
1.251PheGln: 1.251 ± 0.272
1.84PheArg: 1.84 ± 0.312
2.282PheSer: 2.282 ± 0.386
1.766PheThr: 1.766 ± 0.299
1.914PheVal: 1.914 ± 0.374
0.442PheTrp: 0.442 ± 0.176
0.883PheTyr: 0.883 ± 0.18
0.0PheXaa: 0.0 ± 0.0
Gly
6.918GlyAla: 6.918 ± 0.98
0.662GlyCys: 0.662 ± 0.181
5.446GlyAsp: 5.446 ± 0.955
4.416GlyGlu: 4.416 ± 0.623
3.974GlyPhe: 3.974 ± 0.517
7.581GlyGly: 7.581 ± 1.064
1.766GlyHis: 1.766 ± 0.332
3.901GlyIle: 3.901 ± 0.533
2.797GlyLys: 2.797 ± 0.417
6.477GlyLeu: 6.477 ± 0.841
2.134GlyMet: 2.134 ± 0.401
3.533GlyAsn: 3.533 ± 0.681
6.771GlyPro: 6.771 ± 3.509
3.533GlyGln: 3.533 ± 0.507
4.784GlyArg: 4.784 ± 0.556
4.858GlySer: 4.858 ± 0.72
5.373GlyThr: 5.373 ± 0.768
5.152GlyVal: 5.152 ± 0.523
1.398GlyTrp: 1.398 ± 0.274
2.429GlyTyr: 2.429 ± 0.383
0.0GlyXaa: 0.0 ± 0.0
His
1.693HisAla: 1.693 ± 0.309
0.147HisCys: 0.147 ± 0.1
1.251HisAsp: 1.251 ± 0.307
1.398HisGlu: 1.398 ± 0.292
0.515HisPhe: 0.515 ± 0.165
1.987HisGly: 1.987 ± 0.422
0.442HisHis: 0.442 ± 0.183
1.178HisIle: 1.178 ± 0.212
0.662HisLys: 0.662 ± 0.231
1.472HisLeu: 1.472 ± 0.392
0.221HisMet: 0.221 ± 0.128
0.662HisAsn: 0.662 ± 0.191
1.398HisPro: 1.398 ± 0.313
1.03HisGln: 1.03 ± 0.288
1.84HisArg: 1.84 ± 0.335
0.515HisSer: 0.515 ± 0.258
0.957HisThr: 0.957 ± 0.219
0.957HisVal: 0.957 ± 0.274
0.221HisTrp: 0.221 ± 0.14
0.883HisTyr: 0.883 ± 0.227
0.0HisXaa: 0.0 ± 0.0
Ile
4.858IleAla: 4.858 ± 0.616
0.442IleCys: 0.442 ± 0.147
3.68IleAsp: 3.68 ± 0.472
3.606IleGlu: 3.606 ± 0.572
1.03IlePhe: 1.03 ± 0.245
4.122IleGly: 4.122 ± 0.482
1.03IleHis: 1.03 ± 0.263
1.766IleIle: 1.766 ± 0.352
1.178IleLys: 1.178 ± 0.27
2.355IleLeu: 2.355 ± 0.481
0.662IleMet: 0.662 ± 0.3
1.766IleAsn: 1.766 ± 0.315
3.754IlePro: 3.754 ± 0.391
1.84IleGln: 1.84 ± 0.455
3.459IleArg: 3.459 ± 0.416
2.502IleSer: 2.502 ± 0.566
3.827IleThr: 3.827 ± 0.451
3.533IleVal: 3.533 ± 0.484
0.736IleTrp: 0.736 ± 0.213
1.472IleTyr: 1.472 ± 0.29
0.0IleXaa: 0.0 ± 0.0
Lys
3.974LysAla: 3.974 ± 0.551
0.074LysCys: 0.074 ± 0.076
2.282LysAsp: 2.282 ± 0.41
2.65LysGlu: 2.65 ± 0.494
0.883LysPhe: 0.883 ± 0.283
3.901LysGly: 3.901 ± 0.919
0.883LysHis: 0.883 ± 0.272
1.987LysIle: 1.987 ± 0.383
2.502LysLys: 2.502 ± 0.481
3.165LysLeu: 3.165 ± 0.488
0.957LysMet: 0.957 ± 0.225
1.178LysAsn: 1.178 ± 0.26
2.355LysPro: 2.355 ± 0.517
1.178LysGln: 1.178 ± 0.23
2.723LysArg: 2.723 ± 0.538
2.355LysSer: 2.355 ± 0.45
2.355LysThr: 2.355 ± 0.501
3.827LysVal: 3.827 ± 0.574
0.81LysTrp: 0.81 ± 0.276
1.251LysTyr: 1.251 ± 0.318
0.0LysXaa: 0.0 ± 0.0
Leu
8.17LeuAla: 8.17 ± 0.852
0.294LeuCys: 0.294 ± 0.157
6.109LeuAsp: 6.109 ± 0.65
6.109LeuGlu: 6.109 ± 0.66
2.208LeuPhe: 2.208 ± 0.326
6.035LeuGly: 6.035 ± 0.913
1.325LeuHis: 1.325 ± 0.393
4.931LeuIle: 4.931 ± 0.544
3.312LeuLys: 3.312 ± 0.386
6.256LeuLeu: 6.256 ± 0.685
2.429LeuMet: 2.429 ± 0.402
3.018LeuAsn: 3.018 ± 0.417
4.122LeuPro: 4.122 ± 0.592
2.429LeuGln: 2.429 ± 0.761
6.771LeuArg: 6.771 ± 0.699
4.269LeuSer: 4.269 ± 0.516
5.299LeuThr: 5.299 ± 0.718
4.71LeuVal: 4.71 ± 0.491
1.325LeuTrp: 1.325 ± 0.297
2.576LeuTyr: 2.576 ± 0.56
0.0LeuXaa: 0.0 ± 0.0
Met
3.386MetAla: 3.386 ± 0.586
0.074MetCys: 0.074 ± 0.071
1.84MetAsp: 1.84 ± 0.351
1.398MetGlu: 1.398 ± 0.352
0.883MetPhe: 0.883 ± 0.24
1.546MetGly: 1.546 ± 0.386
0.294MetHis: 0.294 ± 0.167
1.546MetIle: 1.546 ± 0.309
1.693MetLys: 1.693 ± 0.317
1.325MetLeu: 1.325 ± 0.217
0.442MetMet: 0.442 ± 0.163
1.178MetAsn: 1.178 ± 0.299
1.766MetPro: 1.766 ± 0.387
0.515MetGln: 0.515 ± 0.226
1.398MetArg: 1.398 ± 0.378
1.472MetSer: 1.472 ± 0.322
1.325MetThr: 1.325 ± 0.301
1.693MetVal: 1.693 ± 0.35
0.221MetTrp: 0.221 ± 0.131
0.515MetTyr: 0.515 ± 0.192
0.0MetXaa: 0.0 ± 0.0
Asn
3.606AsnAla: 3.606 ± 0.442
0.368AsnCys: 0.368 ± 0.152
1.693AsnAsp: 1.693 ± 0.343
1.84AsnGlu: 1.84 ± 0.4
1.472AsnPhe: 1.472 ± 0.401
3.827AsnGly: 3.827 ± 0.808
1.03AsnHis: 1.03 ± 0.225
1.987AsnIle: 1.987 ± 0.539
1.104AsnLys: 1.104 ± 0.311
2.502AsnLeu: 2.502 ± 0.45
0.957AsnMet: 0.957 ± 0.285
1.104AsnAsn: 1.104 ± 0.3
2.576AsnPro: 2.576 ± 0.524
1.398AsnGln: 1.398 ± 0.288
2.134AsnArg: 2.134 ± 0.4
1.472AsnSer: 1.472 ± 0.336
1.84AsnThr: 1.84 ± 0.312
2.87AsnVal: 2.87 ± 0.521
0.81AsnTrp: 0.81 ± 0.234
1.472AsnTyr: 1.472 ± 0.354
0.0AsnXaa: 0.0 ± 0.0
Pro
5.373ProAla: 5.373 ± 0.659
0.368ProCys: 0.368 ± 0.177
3.238ProAsp: 3.238 ± 0.49
4.122ProGlu: 4.122 ± 0.585
1.619ProPhe: 1.619 ± 0.316
5.299ProGly: 5.299 ± 0.845
1.251ProHis: 1.251 ± 0.257
2.502ProIle: 2.502 ± 0.349
3.018ProLys: 3.018 ± 0.67
4.563ProLeu: 4.563 ± 0.706
1.104ProMet: 1.104 ± 0.304
2.944ProAsn: 2.944 ± 0.615
3.312ProPro: 3.312 ± 0.647
3.754ProGln: 3.754 ± 2.068
3.238ProArg: 3.238 ± 0.582
3.238ProSer: 3.238 ± 0.397
3.533ProThr: 3.533 ± 0.65
4.048ProVal: 4.048 ± 0.555
0.883ProTrp: 0.883 ± 0.36
1.178ProTyr: 1.178 ± 0.319
0.0ProXaa: 0.0 ± 0.0
Gln
4.342GlnAla: 4.342 ± 0.631
0.147GlnCys: 0.147 ± 0.1
1.546GlnAsp: 1.546 ± 0.268
2.208GlnGlu: 2.208 ± 0.5
1.546GlnPhe: 1.546 ± 0.355
5.152GlnGly: 5.152 ± 2.912
0.736GlnHis: 0.736 ± 0.201
1.84GlnIle: 1.84 ± 0.388
1.178GlnLys: 1.178 ± 0.294
3.901GlnLeu: 3.901 ± 0.591
1.325GlnMet: 1.325 ± 0.389
0.81GlnAsn: 0.81 ± 0.221
1.766GlnPro: 1.766 ± 0.423
2.355GlnGln: 2.355 ± 0.602
2.65GlnArg: 2.65 ± 0.501
2.061GlnSer: 2.061 ± 0.374
2.134GlnThr: 2.134 ± 0.427
2.797GlnVal: 2.797 ± 0.453
0.81GlnTrp: 0.81 ± 0.221
0.662GlnTyr: 0.662 ± 0.186
0.0GlnXaa: 0.0 ± 0.0
Arg
5.152ArgAla: 5.152 ± 0.641
0.736ArgCys: 0.736 ± 0.271
3.533ArgAsp: 3.533 ± 0.532
4.858ArgGlu: 4.858 ± 0.538
2.282ArgPhe: 2.282 ± 0.443
4.195ArgGly: 4.195 ± 0.603
1.104ArgHis: 1.104 ± 0.317
3.68ArgIle: 3.68 ± 0.405
3.754ArgLys: 3.754 ± 0.656
5.078ArgLeu: 5.078 ± 0.667
2.134ArgMet: 2.134 ± 0.354
2.502ArgAsn: 2.502 ± 0.428
3.238ArgPro: 3.238 ± 0.438
2.208ArgGln: 2.208 ± 0.377
5.078ArgArg: 5.078 ± 0.703
4.195ArgSer: 4.195 ± 0.657
3.533ArgThr: 3.533 ± 0.59
4.71ArgVal: 4.71 ± 0.548
1.84ArgTrp: 1.84 ± 0.409
1.987ArgTyr: 1.987 ± 0.423
0.0ArgXaa: 0.0 ± 0.0
Ser
4.195SerAla: 4.195 ± 0.617
0.074SerCys: 0.074 ± 0.07
2.576SerAsp: 2.576 ± 0.356
4.048SerGlu: 4.048 ± 0.53
2.87SerPhe: 2.87 ± 0.443
5.52SerGly: 5.52 ± 0.747
1.104SerHis: 1.104 ± 0.256
2.944SerIle: 2.944 ± 0.476
2.282SerLys: 2.282 ± 0.602
3.901SerLeu: 3.901 ± 0.63
1.693SerMet: 1.693 ± 0.313
1.84SerAsn: 1.84 ± 0.354
2.208SerPro: 2.208 ± 0.345
2.134SerGln: 2.134 ± 0.482
3.754SerArg: 3.754 ± 0.598
2.429SerSer: 2.429 ± 0.408
2.723SerThr: 2.723 ± 0.433
4.342SerVal: 4.342 ± 0.579
1.251SerTrp: 1.251 ± 0.352
1.693SerTyr: 1.693 ± 0.317
0.0SerXaa: 0.0 ± 0.0
Thr
5.888ThrAla: 5.888 ± 0.5
0.515ThrCys: 0.515 ± 0.213
3.606ThrAsp: 3.606 ± 0.5
3.754ThrGlu: 3.754 ± 0.558
1.84ThrPhe: 1.84 ± 0.384
5.373ThrGly: 5.373 ± 1.302
1.03ThrHis: 1.03 ± 0.312
2.355ThrIle: 2.355 ± 0.541
2.355ThrLys: 2.355 ± 0.397
5.594ThrLeu: 5.594 ± 0.645
1.104ThrMet: 1.104 ± 0.227
1.472ThrAsn: 1.472 ± 0.517
4.195ThrPro: 4.195 ± 0.512
2.65ThrGln: 2.65 ± 0.46
3.827ThrArg: 3.827 ± 0.541
2.87ThrSer: 2.87 ± 0.633
3.238ThrThr: 3.238 ± 0.55
4.637ThrVal: 4.637 ± 0.496
1.104ThrTrp: 1.104 ± 0.264
1.84ThrTyr: 1.84 ± 0.324
0.0ThrXaa: 0.0 ± 0.0
Val
7.286ValAla: 7.286 ± 0.855
0.589ValCys: 0.589 ± 0.193
4.784ValAsp: 4.784 ± 0.705
5.594ValGlu: 5.594 ± 0.816
2.355ValPhe: 2.355 ± 0.552
4.563ValGly: 4.563 ± 0.523
1.178ValHis: 1.178 ± 0.304
3.238ValIle: 3.238 ± 0.57
3.754ValLys: 3.754 ± 0.51
5.52ValLeu: 5.52 ± 0.609
1.472ValMet: 1.472 ± 0.339
3.018ValAsn: 3.018 ± 0.49
4.269ValPro: 4.269 ± 0.568
2.576ValGln: 2.576 ± 0.468
5.078ValArg: 5.078 ± 0.694
3.606ValSer: 3.606 ± 0.431
3.901ValThr: 3.901 ± 0.554
6.182ValVal: 6.182 ± 0.64
1.03ValTrp: 1.03 ± 0.289
2.355ValTyr: 2.355 ± 0.449
0.0ValXaa: 0.0 ± 0.0
Trp
1.914TrpAla: 1.914 ± 0.367
0.368TrpCys: 0.368 ± 0.196
1.03TrpAsp: 1.03 ± 0.292
0.736TrpGlu: 0.736 ± 0.201
0.883TrpPhe: 0.883 ± 0.241
1.398TrpGly: 1.398 ± 0.284
0.294TrpHis: 0.294 ± 0.137
1.619TrpIle: 1.619 ± 0.318
0.736TrpLys: 0.736 ± 0.188
1.325TrpLeu: 1.325 ± 0.312
0.736TrpMet: 0.736 ± 0.172
0.515TrpAsn: 0.515 ± 0.196
0.883TrpPro: 0.883 ± 0.288
0.81TrpGln: 0.81 ± 0.227
1.104TrpArg: 1.104 ± 0.301
0.883TrpSer: 0.883 ± 0.284
1.178TrpThr: 1.178 ± 0.267
1.472TrpVal: 1.472 ± 0.234
0.589TrpTrp: 0.589 ± 0.172
0.442TrpTyr: 0.442 ± 0.192
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.576TyrAla: 2.576 ± 0.437
0.221TyrCys: 0.221 ± 0.116
2.355TyrAsp: 2.355 ± 0.469
2.134TyrGlu: 2.134 ± 0.446
0.736TyrPhe: 0.736 ± 0.222
1.693TyrGly: 1.693 ± 0.349
0.589TyrHis: 0.589 ± 0.184
1.251TyrIle: 1.251 ± 0.309
0.81TyrLys: 0.81 ± 0.262
3.312TyrLeu: 3.312 ± 0.492
1.178TyrMet: 1.178 ± 0.297
0.957TyrAsn: 0.957 ± 0.243
1.619TyrPro: 1.619 ± 0.314
1.104TyrGln: 1.104 ± 0.211
2.208TyrArg: 2.208 ± 0.413
1.325TyrSer: 1.325 ± 0.261
1.84TyrThr: 1.84 ± 0.275
2.208TyrVal: 2.208 ± 0.375
0.736TyrTrp: 0.736 ± 0.209
0.883TyrTyr: 0.883 ± 0.326
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 73 proteins (13588 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski