Pseudonocardia sp. EC080619-01

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Pseudonocardia; unclassified Pseudonocardia

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5906 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0P0SKX9|A0A0P0SKX9_9PSEU 2-hydroxy-6-oxo-6-phenylhexa-2 4-dienoate hydrolase OS=Pseudonocardia sp. EC080619-01 OX=1096856 GN=AD017_29170 PE=4 SV=1
MM1 pKa = 7.83PATLVIALGVSAALTFTEE19 pKa = 5.08LPSAPVAQAAGPIDD33 pKa = 3.76ATAAAPTPVRR43 pKa = 11.84RR44 pKa = 11.84LVPPPPLPARR54 pKa = 11.84VIPDD58 pKa = 4.0FPLPQPVTTEE68 pKa = 4.31SEE70 pKa = 4.33PVTYY74 pKa = 9.75TCDD77 pKa = 3.4PTGDD81 pKa = 4.08PEE83 pKa = 5.3FGDD86 pKa = 3.81PANPHH91 pKa = 7.24LITNKK96 pKa = 10.23DD97 pKa = 3.63CPALNAAKK105 pKa = 9.92EE106 pKa = 4.07QAQRR110 pKa = 11.84EE111 pKa = 4.25YY112 pKa = 11.33LEE114 pKa = 4.09QLNSDD119 pKa = 3.61VVGDD123 pKa = 3.78HH124 pKa = 7.42RR125 pKa = 11.84EE126 pKa = 4.24YY127 pKa = 10.56TCSDD131 pKa = 3.96PSSSDD136 pKa = 3.31YY137 pKa = 10.01GTAACGTDD145 pKa = 3.16ADD147 pKa = 4.94GDD149 pKa = 4.08GLMDD153 pKa = 5.57GGPLDD158 pKa = 3.69TDD160 pKa = 3.59

Molecular weight: 16.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0P0SLG2|A0A0P0SLG2_9PSEU Amidohydro-rel domain-containing protein OS=Pseudonocardia sp. EC080619-01 OX=1096856 GN=AD017_30470 PE=4 SV=1
MM1 pKa = 7.24MLPRR5 pKa = 11.84TVTEE9 pKa = 4.07AVAILATSAKK19 pKa = 8.81PLVVGGGTLCVPALARR35 pKa = 11.84GEE37 pKa = 4.17IVPTDD42 pKa = 3.59VVDD45 pKa = 4.56LGRR48 pKa = 11.84AGLDD52 pKa = 3.4TIVDD56 pKa = 3.78GRR58 pKa = 11.84SVEE61 pKa = 3.88IGALVSYY68 pKa = 9.93QQLITSTVVRR78 pKa = 11.84RR79 pKa = 11.84EE80 pKa = 3.82LPLLHH85 pKa = 6.95RR86 pKa = 11.84LASGITGGIQIRR98 pKa = 11.84HH99 pKa = 5.55QGTLVGALCAARR111 pKa = 11.84PQSDD115 pKa = 3.68VPAAVVALRR124 pKa = 11.84AEE126 pKa = 4.28VLVRR130 pKa = 11.84SATRR134 pKa = 11.84TRR136 pKa = 11.84TVPAEE141 pKa = 3.98RR142 pKa = 11.84FLAGPMAPDD151 pKa = 4.39LAPDD155 pKa = 3.58EE156 pKa = 4.85LVVGVRR162 pKa = 11.84IPKK165 pKa = 10.39CPDD168 pKa = 2.38GSGYY172 pKa = 10.58VKK174 pKa = 10.61HH175 pKa = 6.55KK176 pKa = 9.95FAEE179 pKa = 4.5SSWPVVTAAVVPGRR193 pKa = 11.84VVLGGVAGTPQVVRR207 pKa = 11.84LPAGVTAGATVRR219 pKa = 11.84ASVTRR224 pKa = 11.84RR225 pKa = 11.84LAALPAEE232 pKa = 4.79HH233 pKa = 6.89RR234 pKa = 11.84WSDD237 pKa = 4.14LRR239 pKa = 11.84APWDD243 pKa = 3.29YY244 pKa = 10.72RR245 pKa = 11.84RR246 pKa = 11.84RR247 pKa = 11.84VAPEE251 pKa = 3.2IAVRR255 pKa = 11.84AVEE258 pKa = 4.15QVKK261 pKa = 7.75EE262 pKa = 4.3TRR264 pKa = 3.56

Molecular weight: 27.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5906

0

5906

1852929

28

7371

313.7

33.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.899 ± 0.05

0.74 ± 0.008

6.517 ± 0.028

5.418 ± 0.032

2.671 ± 0.019

10.092 ± 0.032

2.225 ± 0.018

3.073 ± 0.022

1.344 ± 0.021

10.358 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.694 ± 0.012

1.484 ± 0.017

6.253 ± 0.026

2.358 ± 0.02

8.339 ± 0.035

4.772 ± 0.023

6.067 ± 0.023

9.492 ± 0.038

1.44 ± 0.015

1.765 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski