Amino acid dipepetide frequency for Pseudonocardia sp. EC080619-01

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	22.373AlaAla: 22.373 ± 0.183	1.031AlaCys: 1.031 ± 0.022	9.064AlaAsp: 9.064 ± 0.077	8.664AlaGlu: 8.664 ± 0.083	3.52AlaPhe: 3.52 ± 0.044	15.78AlaGly: 15.78 ± 0.11	2.738AlaHis: 2.738 ± 0.041	3.733AlaIle: 3.733 ± 0.049	1.681AlaLys: 1.681 ± 0.039	14.239AlaLeu: 14.239 ± 0.139	2.498AlaMet: 2.498 ± 0.035	1.736AlaAsn: 1.736 ± 0.031	7.341AlaPro: 7.341 ± 0.078	3.116AlaGln: 3.116 ± 0.051	10.781AlaArg: 10.781 ± 0.089	5.583AlaSer: 5.583 ± 0.048	7.561AlaThr: 7.561 ± 0.065	13.737AlaVal: 13.737 ± 0.119	1.8AlaTrp: 1.8 ± 0.033	2.011AlaTyr: 2.011 ± 0.031	0.0AlaXaa: 0.0 ± 0.0
Cys	1.041CysAla: 1.041 ± 0.025	0.084CysCys: 0.084 ± 0.008	0.495CysAsp: 0.495 ± 0.017	0.384CysGlu: 0.384 ± 0.015	0.198CysPhe: 0.198 ± 0.011	0.944CysGly: 0.944 ± 0.026	0.176CysHis: 0.176 ± 0.01	0.169CysIle: 0.169 ± 0.011	0.078CysLys: 0.078 ± 0.007	0.645CysLeu: 0.645 ± 0.017	0.091CysMet: 0.091 ± 0.007	0.103CysAsn: 0.103 ± 0.007	0.47CysPro: 0.47 ± 0.019	0.114CysGln: 0.114 ± 0.008	0.623CysArg: 0.623 ± 0.017	0.42CysSer: 0.42 ± 0.014	0.488CysThr: 0.488 ± 0.015	0.62CysVal: 0.62 ± 0.021	0.107CysTrp: 0.107 ± 0.007	0.149CysTyr: 0.149 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	8.789AspAla: 8.789 ± 0.071	0.356AspCys: 0.356 ± 0.014	4.966AspAsp: 4.966 ± 0.065	4.155AspGlu: 4.155 ± 0.05	1.302AspPhe: 1.302 ± 0.028	7.363AspGly: 7.363 ± 0.065	1.515AspHis: 1.515 ± 0.029	1.488AspIle: 1.488 ± 0.032	0.704AspLys: 0.704 ± 0.024	6.772AspLeu: 6.772 ± 0.064	0.784AspMet: 0.784 ± 0.018	0.695AspAsn: 0.695 ± 0.022	5.526AspPro: 5.526 ± 0.058	1.406AspGln: 1.406 ± 0.029	6.145AspArg: 6.145 ± 0.057	2.109AspSer: 2.109 ± 0.037	3.268AspThr: 3.268 ± 0.042	5.919AspVal: 5.919 ± 0.061	0.878AspTrp: 0.878 ± 0.021	1.026AspTyr: 1.026 ± 0.026	0.0AspXaa: 0.0 ± 0.0
Glu	6.21GluAla: 6.21 ± 0.076	0.304GluCys: 0.304 ± 0.012	2.445GluAsp: 2.445 ± 0.037	2.601GluGlu: 2.601 ± 0.041	1.573GluPhe: 1.573 ± 0.029	3.609GluGly: 3.609 ± 0.049	1.7GluHis: 1.7 ± 0.031	2.581GluIle: 2.581 ± 0.038	1.121GluLys: 1.121 ± 0.033	7.156GluLeu: 7.156 ± 0.072	0.89GluMet: 0.89 ± 0.024	0.919GluAsn: 0.919 ± 0.023	3.553GluPro: 3.553 ± 0.05	2.318GluGln: 2.318 ± 0.036	5.559GluArg: 5.559 ± 0.059	2.432GluSer: 2.432 ± 0.038	2.845GluThr: 2.845 ± 0.041	4.658GluVal: 4.658 ± 0.05	0.769GluTrp: 0.769 ± 0.02	0.933GluTyr: 0.933 ± 0.025	0.0GluXaa: 0.0 ± 0.0
Phe	3.82PheAla: 3.82 ± 0.052	0.281PheCys: 0.281 ± 0.012	2.053PheAsp: 2.053 ± 0.042	1.197PheGlu: 1.197 ± 0.029	0.789PhePhe: 0.789 ± 0.024	3.284PheGly: 3.284 ± 0.048	0.613PheHis: 0.613 ± 0.021	0.502PheIle: 0.502 ± 0.019	0.28PheLys: 0.28 ± 0.012	2.577PheLeu: 2.577 ± 0.044	0.332PheMet: 0.332 ± 0.013	0.399PheAsn: 0.399 ± 0.014	1.392PhePro: 1.392 ± 0.032	0.526PheGln: 0.526 ± 0.015	1.869PheArg: 1.869 ± 0.035	1.357PheSer: 1.357 ± 0.029	1.864PheThr: 1.864 ± 0.036	2.61PheVal: 2.61 ± 0.04	0.422PheTrp: 0.422 ± 0.014	0.539PheTyr: 0.539 ± 0.018	0.0PheXaa: 0.0 ± 0.0
Gly	12.506GlyAla: 12.506 ± 0.088	0.808GlyCys: 0.808 ± 0.025	5.835GlyAsp: 5.835 ± 0.061	5.293GlyGlu: 5.293 ± 0.056	3.091GlyPhe: 3.091 ± 0.041	9.463GlyGly: 9.463 ± 0.085	2.292GlyHis: 2.292 ± 0.04	3.762GlyIle: 3.762 ± 0.054	1.539GlyLys: 1.539 ± 0.032	10.153GlyLeu: 10.153 ± 0.089	2.171GlyMet: 2.171 ± 0.034	1.53GlyAsn: 1.53 ± 0.029	6.275GlyPro: 6.275 ± 0.064	2.375GlyGln: 2.375 ± 0.038	8.631GlyArg: 8.631 ± 0.069	5.504GlySer: 5.504 ± 0.056	6.859GlyThr: 6.859 ± 0.067	8.77GlyVal: 8.77 ± 0.062	1.802GlyTrp: 1.802 ± 0.034	2.263GlyTyr: 2.263 ± 0.041	0.0GlyXaa: 0.0 ± 0.0
His	2.631HisAla: 2.631 ± 0.047	0.188HisCys: 0.188 ± 0.01	1.607HisAsp: 1.607 ± 0.033	1.157HisGlu: 1.157 ± 0.029	0.527HisPhe: 0.527 ± 0.018	2.509HisGly: 2.509 ± 0.041	0.708HisHis: 0.708 ± 0.021	0.443HisIle: 0.443 ± 0.016	0.214HisLys: 0.214 ± 0.013	2.377HisLeu: 2.377 ± 0.039	0.277HisMet: 0.277 ± 0.012	0.318HisAsn: 0.318 ± 0.014	1.756HisPro: 1.756 ± 0.028	0.492HisGln: 0.492 ± 0.016	2.221HisArg: 2.221 ± 0.04	0.908HisSer: 0.908 ± 0.024	1.167HisThr: 1.167 ± 0.024	1.951HisVal: 1.951 ± 0.037	0.332HisTrp: 0.332 ± 0.015	0.467HisTyr: 0.467 ± 0.019	0.0HisXaa: 0.0 ± 0.0
Ile	4.878IleAla: 4.878 ± 0.055	0.264IleCys: 0.264 ± 0.013	2.393IleAsp: 2.393 ± 0.036	1.902IleGlu: 1.902 ± 0.037	0.648IlePhe: 0.648 ± 0.023	3.926IleGly: 3.926 ± 0.048	0.491IleHis: 0.491 ± 0.017	0.704IleIle: 0.704 ± 0.024	0.454IleLys: 0.454 ± 0.017	2.213IleLeu: 2.213 ± 0.045	0.43IleMet: 0.43 ± 0.015	0.565IleAsn: 0.565 ± 0.019	1.614IlePro: 1.614 ± 0.032	0.56IleGln: 0.56 ± 0.016	2.109IleArg: 2.109 ± 0.034	1.542IleSer: 1.542 ± 0.029	2.118IleThr: 2.118 ± 0.034	3.073IleVal: 3.073 ± 0.05	0.356IleTrp: 0.356 ± 0.015	0.496IleTyr: 0.496 ± 0.017	0.0IleXaa: 0.0 ± 0.0
Lys	1.709LysAla: 1.709 ± 0.042	0.068LysCys: 0.068 ± 0.006	0.791LysAsp: 0.791 ± 0.02	0.693LysGlu: 0.693 ± 0.023	0.324LysPhe: 0.324 ± 0.015	1.049LysGly: 1.049 ± 0.029	0.301LysHis: 0.301 ± 0.012	0.679LysIle: 0.679 ± 0.02	0.397LysLys: 0.397 ± 0.019	1.427LysLeu: 1.427 ± 0.032	0.261LysMet: 0.261 ± 0.014	0.276LysAsn: 0.276 ± 0.014	0.841LysPro: 0.841 ± 0.025	0.45LysGln: 0.45 ± 0.015	1.015LysArg: 1.015 ± 0.03	0.69LysSer: 0.69 ± 0.021	0.752LysThr: 0.752 ± 0.021	1.317LysVal: 1.317 ± 0.033	0.148LysTrp: 0.148 ± 0.01	0.254LysTyr: 0.254 ± 0.012	0.0LysXaa: 0.0 ± 0.0
Leu	15.804LeuAla: 15.804 ± 0.11	0.778LeuCys: 0.778 ± 0.02	7.514LeuAsp: 7.514 ± 0.07	4.554LeuGlu: 4.554 ± 0.058	2.606LeuPhe: 2.606 ± 0.044	10.125LeuGly: 10.125 ± 0.093	2.335LeuHis: 2.335 ± 0.037	2.707LeuIle: 2.707 ± 0.051	1.101LeuLys: 1.101 ± 0.034	11.465LeuLeu: 11.465 ± 0.12	1.411LeuMet: 1.411 ± 0.026	1.381LeuAsn: 1.381 ± 0.03	6.367LeuPro: 6.367 ± 0.062	2.126LeuGln: 2.126 ± 0.032	8.786LeuArg: 8.786 ± 0.083	5.021LeuSer: 5.021 ± 0.054	6.343LeuThr: 6.343 ± 0.057	10.47LeuVal: 10.47 ± 0.101	1.165LeuTrp: 1.165 ± 0.03	1.525LeuTyr: 1.525 ± 0.03	0.0LeuXaa: 0.0 ± 0.0
Met	2.098MetAla: 2.098 ± 0.037	0.122MetCys: 0.122 ± 0.008	0.829MetAsp: 0.829 ± 0.019	0.647MetGlu: 0.647 ± 0.019	0.516MetPhe: 0.516 ± 0.016	1.26MetGly: 1.26 ± 0.028	0.349MetHis: 0.349 ± 0.015	0.754MetIle: 0.754 ± 0.022	0.296MetLys: 0.296 ± 0.012	1.817MetLeu: 1.817 ± 0.033	0.266MetMet: 0.266 ± 0.014	0.358MetAsn: 0.358 ± 0.014	1.071MetPro: 1.071 ± 0.023	0.434MetGln: 0.434 ± 0.015	1.389MetArg: 1.389 ± 0.032	1.291MetSer: 1.291 ± 0.024	1.635MetThr: 1.635 ± 0.03	1.363MetVal: 1.363 ± 0.029	0.189MetTrp: 0.189 ± 0.011	0.252MetTyr: 0.252 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	1.899AsnAla: 1.899 ± 0.033	0.126AsnCys: 0.126 ± 0.008	0.847AsnAsp: 0.847 ± 0.022	0.651AsnGlu: 0.651 ± 0.019	0.436AsnPhe: 0.436 ± 0.015	1.497AsnGly: 1.497 ± 0.032	0.328AsnHis: 0.328 ± 0.013	0.521AsnIle: 0.521 ± 0.019	0.241AsnLys: 0.241 ± 0.012	1.524AsnLeu: 1.524 ± 0.029	0.251AsnMet: 0.251 ± 0.013	0.312AsnAsn: 0.312 ± 0.014	1.227AsnPro: 1.227 ± 0.027	0.396AsnGln: 0.396 ± 0.017	1.182AsnArg: 1.182 ± 0.028	0.695AsnSer: 0.695 ± 0.021	0.848AsnThr: 0.848 ± 0.02	1.308AsnVal: 1.308 ± 0.029	0.226AsnTrp: 0.226 ± 0.012	0.322AsnTyr: 0.322 ± 0.014	0.0AsnXaa: 0.0 ± 0.0
Pro	9.305ProAla: 9.305 ± 0.091	0.325ProCys: 0.325 ± 0.013	5.399ProAsp: 5.399 ± 0.057	4.261ProGlu: 4.261 ± 0.043	1.522ProPhe: 1.522 ± 0.03	7.566ProGly: 7.566 ± 0.079	1.317ProHis: 1.317 ± 0.027	1.426ProIle: 1.426 ± 0.026	0.773ProLys: 0.773 ± 0.024	5.184ProLeu: 5.184 ± 0.058	1.021ProMet: 1.021 ± 0.023	0.795ProAsn: 0.795 ± 0.024	3.591ProPro: 3.591 ± 0.06	1.499ProGln: 1.499 ± 0.033	4.074ProArg: 4.074 ± 0.05	3.118ProSer: 3.118 ± 0.039	3.165ProThr: 3.165 ± 0.046	6.236ProVal: 6.236 ± 0.066	0.926ProTrp: 0.926 ± 0.024	1.029ProTyr: 1.029 ± 0.024	0.0ProXaa: 0.0 ± 0.0
Gln	3.103GlnAla: 3.103 ± 0.046	0.15GlnCys: 0.15 ± 0.01	1.264GlnAsp: 1.264 ± 0.029	1.104GlnGlu: 1.104 ± 0.025	0.614GlnPhe: 0.614 ± 0.022	1.915GlnGly: 1.915 ± 0.037	0.531GlnHis: 0.531 ± 0.016	0.969GlnIle: 0.969 ± 0.022	0.427GlnLys: 0.427 ± 0.018	2.733GlnLeu: 2.733 ± 0.043	0.458GlnMet: 0.458 ± 0.016	0.466GlnAsn: 0.466 ± 0.019	1.295GlnPro: 1.295 ± 0.031	1.002GlnGln: 1.002 ± 0.024	2.214GlnArg: 2.214 ± 0.037	0.986GlnSer: 0.986 ± 0.021	1.117GlnThr: 1.117 ± 0.03	2.407GlnVal: 2.407 ± 0.038	0.359GlnTrp: 0.359 ± 0.014	0.47GlnTyr: 0.47 ± 0.017	0.0GlnXaa: 0.0 ± 0.0
Arg	10.636ArgAla: 10.636 ± 0.094	0.624ArgCys: 0.624 ± 0.021	5.057ArgAsp: 5.057 ± 0.055	4.72ArgGlu: 4.72 ± 0.058	2.523ArgPhe: 2.523 ± 0.037	6.341ArgGly: 6.341 ± 0.052	2.005ArgHis: 2.005 ± 0.032	3.513ArgIle: 3.513 ± 0.043	1.139ArgLys: 1.139 ± 0.027	8.404ArgLeu: 8.404 ± 0.076	1.906ArgMet: 1.906 ± 0.034	1.311ArgAsn: 1.311 ± 0.03	5.296ArgPro: 5.296 ± 0.052	1.798ArgGln: 1.798 ± 0.036	8.334ArgArg: 8.334 ± 0.095	4.434ArgSer: 4.434 ± 0.05	5.484ArgThr: 5.484 ± 0.054	6.569ArgVal: 6.569 ± 0.069	1.469ArgTrp: 1.469 ± 0.029	1.831ArgTyr: 1.831 ± 0.031	0.0ArgXaa: 0.0 ± 0.0
Ser	6.172SerAla: 6.172 ± 0.058	0.371SerCys: 0.371 ± 0.015	2.721SerAsp: 2.721 ± 0.042	2.332SerGlu: 2.332 ± 0.035	1.438SerPhe: 1.438 ± 0.032	6.041SerGly: 6.041 ± 0.06	0.854SerHis: 0.854 ± 0.021	1.448SerIle: 1.448 ± 0.026	0.67SerLys: 0.67 ± 0.022	4.375SerLeu: 4.375 ± 0.051	1.137SerMet: 1.137 ± 0.025	0.733SerAsn: 0.733 ± 0.02	3.013SerPro: 3.013 ± 0.038	1.016SerGln: 1.016 ± 0.025	3.622SerArg: 3.622 ± 0.047	2.655SerSer: 2.655 ± 0.057	3.068SerThr: 3.068 ± 0.039	4.225SerVal: 4.225 ± 0.052	0.849SerTrp: 0.849 ± 0.022	0.977SerTyr: 0.977 ± 0.026	0.0SerXaa: 0.0 ± 0.0
Thr	8.673ThrAla: 8.673 ± 0.076	0.427ThrCys: 0.427 ± 0.017	3.835ThrAsp: 3.835 ± 0.049	3.183ThrGlu: 3.183 ± 0.051	1.568ThrPhe: 1.568 ± 0.029	7.382ThrGly: 7.382 ± 0.066	1.137ThrHis: 1.137 ± 0.027	1.831ThrIle: 1.831 ± 0.032	0.737ThrLys: 0.737 ± 0.024	5.581ThrLeu: 5.581 ± 0.052	1.01ThrMet: 1.01 ± 0.022	0.841ThrAsn: 0.841 ± 0.024	4.023ThrPro: 4.023 ± 0.047	1.032ThrGln: 1.032 ± 0.026	4.152ThrArg: 4.152 ± 0.041	2.828ThrSer: 2.828 ± 0.039	3.943ThrThr: 3.943 ± 0.051	6.543ThrVal: 6.543 ± 0.062	0.89ThrTrp: 0.89 ± 0.022	1.049ThrTyr: 1.049 ± 0.028	0.001ThrXaa: 0.001 ± 0.001
Val	13.447ValAla: 13.447 ± 0.118	0.771ValCys: 0.771 ± 0.024	6.021ValAsp: 6.021 ± 0.062	5.137ValGlu: 5.137 ± 0.058	2.469ValPhe: 2.469 ± 0.039	7.881ValGly: 7.881 ± 0.075	2.169ValHis: 2.169 ± 0.037	2.615ValIle: 2.615 ± 0.041	1.065ValLys: 1.065 ± 0.027	11.289ValLeu: 11.289 ± 0.106	1.264ValMet: 1.264 ± 0.029	1.484ValAsn: 1.484 ± 0.028	6.094ValPro: 6.094 ± 0.06	2.048ValGln: 2.048 ± 0.034	7.704ValArg: 7.704 ± 0.074	4.35ValSer: 4.35 ± 0.045	6.107ValThr: 6.107 ± 0.063	10.555ValVal: 10.555 ± 0.091	1.07ValTrp: 1.07 ± 0.027	1.378ValTyr: 1.378 ± 0.028	0.0ValXaa: 0.0 ± 0.0
Trp	1.648TrpAla: 1.648 ± 0.034	0.14TrpCys: 0.14 ± 0.008	0.813TrpAsp: 0.813 ± 0.021	0.667TrpGlu: 0.667 ± 0.019	0.539TrpPhe: 0.539 ± 0.018	1.069TrpGly: 1.069 ± 0.025	0.326TrpHis: 0.326 ± 0.014	0.54TrpIle: 0.54 ± 0.021	0.253TrpLys: 0.253 ± 0.012	1.647TrpLeu: 1.647 ± 0.031	0.272TrpMet: 0.272 ± 0.012	0.318TrpAsn: 0.318 ± 0.013	0.804TrpPro: 0.804 ± 0.026	0.439TrpGln: 0.439 ± 0.014	1.23TrpArg: 1.23 ± 0.027	0.953TrpSer: 0.953 ± 0.029	1.031TrpThr: 1.031 ± 0.02	1.087TrpVal: 1.087 ± 0.023	0.34TrpTrp: 0.34 ± 0.016	0.278TrpTyr: 0.278 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.247TyrAla: 2.247 ± 0.032	0.18TyrCys: 0.18 ± 0.009	1.216TyrAsp: 1.216 ± 0.031	0.875TyrGlu: 0.875 ± 0.024	0.507TyrPhe: 0.507 ± 0.018	1.918TyrGly: 1.918 ± 0.04	0.366TyrHis: 0.366 ± 0.016	0.345TyrIle: 0.345 ± 0.015	0.268TyrLys: 0.268 ± 0.013	2.004TyrLeu: 2.004 ± 0.036	0.208TyrMet: 0.208 ± 0.011	0.298TyrAsn: 0.298 ± 0.014	0.981TyrPro: 0.981 ± 0.023	0.438TyrGln: 0.438 ± 0.015	1.755TyrArg: 1.755 ± 0.033	0.842TyrSer: 0.842 ± 0.025	1.003TyrThr: 1.003 ± 0.026	1.502TyrVal: 1.502 ± 0.034	0.297TyrTrp: 0.297 ± 0.012	0.398TyrTyr: 0.398 ± 0.016	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5906 proteins (1852929 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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