Paraburkholderia susongensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Paraburkholderia

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6846 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X7KCU6|A0A1X7KCU6_9BURK Methyl-accepting chemotaxis sensory transducer with TarH sensor OS=Paraburkholderia susongensis OX=1515439 GN=SAMN06265784_103569 PE=4 SV=1
MM1 pKa = 7.88RR2 pKa = 11.84ISSAQYY8 pKa = 9.39FSMNVEE14 pKa = 4.21TMSNQQAQLSQIYY27 pKa = 8.43QQVSSGQSLTTPADD41 pKa = 3.65NPLGAAQAVQLSSTAAALSQYY62 pKa = 7.34TTNQSAALASLQAEE76 pKa = 4.52DD77 pKa = 3.85STLSGVLSTLQSVNTLGIRR96 pKa = 11.84ASNGSLNDD104 pKa = 3.67ADD106 pKa = 4.0RR107 pKa = 11.84TAIAAQLTSLRR118 pKa = 11.84DD119 pKa = 3.51QLLTYY124 pKa = 11.26ANATDD129 pKa = 3.6SAGNSLFSGFQNTAQAFTTDD149 pKa = 2.66ASGAVVYY156 pKa = 10.95SGDD159 pKa = 3.31NGTRR163 pKa = 11.84TVQVTGSNQVATGDD177 pKa = 3.28NGRR180 pKa = 11.84AVFMSVGSTNAEE192 pKa = 4.1PIAAGNSANTGTGVIGAVTVNDD214 pKa = 3.69PTQVANADD222 pKa = 3.69SYY224 pKa = 11.53QISFSGSGSTLAYY237 pKa = 9.12TITDD241 pKa = 3.59TTTGVAGSPQAYY253 pKa = 8.32TEE255 pKa = 4.24GSNITLGGQTVAITGTPADD274 pKa = 3.98GDD276 pKa = 3.88NFTVKK281 pKa = 9.97PANQAGTDD289 pKa = 3.41VFANLNAMISALQQPVTGNTAAIANLQNSLNTGLSQLANTLGNVTAVQASVGGRR343 pKa = 11.84EE344 pKa = 3.98QEE346 pKa = 4.23VQALQTVTQTNSLQTQSNLSDD367 pKa = 3.47LTSTDD372 pKa = 3.23MVSAISKK379 pKa = 8.02YY380 pKa = 8.04TLQQAALQASQQAFVQIQNMSLFQYY405 pKa = 10.64INNN408 pKa = 3.99

Molecular weight: 41.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X7K591|A0A1X7K591_9BURK Acetylglutamate kinase OS=Paraburkholderia susongensis OX=1515439 GN=argB PE=3 SV=1
MM1 pKa = 7.11HH2 pKa = 7.28TLLSTRR8 pKa = 11.84LTRR11 pKa = 11.84ASLKK15 pKa = 9.97AARR18 pKa = 11.84PARR21 pKa = 11.84RR22 pKa = 11.84HH23 pKa = 5.14LMRR26 pKa = 11.84ALRR29 pKa = 11.84HH30 pKa = 4.9HH31 pKa = 7.27AARR34 pKa = 11.84TRR36 pKa = 11.84ALGKK40 pKa = 7.98QWRR43 pKa = 11.84DD44 pKa = 2.83ARR46 pKa = 11.84AVDD49 pKa = 5.58GIRR52 pKa = 11.84TWFHH56 pKa = 6.51AFFSALRR63 pKa = 11.84QQSGARR69 pKa = 11.84RR70 pKa = 11.84FSLRR74 pKa = 11.84TLLRR78 pKa = 11.84KK79 pKa = 7.51PTPLRR84 pKa = 11.84LVVAKK89 pKa = 9.89RR90 pKa = 11.84APATPVNTSRR100 pKa = 11.84RR101 pKa = 11.84PRR103 pKa = 11.84RR104 pKa = 11.84LSTSSSGWFAFAFASRR120 pKa = 3.71

Molecular weight: 13.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6846

0

6846

2196354

29

2911

320.8

34.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.661 ± 0.043

0.951 ± 0.011

5.29 ± 0.026

5.127 ± 0.033

3.749 ± 0.019

8.194 ± 0.03

2.318 ± 0.015

4.793 ± 0.022

3.015 ± 0.026

10.251 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.413 ± 0.013

2.889 ± 0.022

5.088 ± 0.026

3.612 ± 0.018

6.881 ± 0.03

5.795 ± 0.025

5.447 ± 0.024

7.709 ± 0.023

1.37 ± 0.014

2.448 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski