Mycobacterium phage Biglebops

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Timquatrovirus; unclassified Timquatrovirus

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z5HC80|A0A2Z5HC80_9CAUD MuF-like minor capsid protein OS=Mycobacterium phage Biglebops OX=2250372 GN=6 PE=4 SV=1
MM1 pKa = 8.09DD2 pKa = 5.43APTHH6 pKa = 5.1FTAVASTGDD15 pKa = 3.51EE16 pKa = 4.33PGWLLVHH23 pKa = 6.26VPEE26 pKa = 5.77IDD28 pKa = 3.29QYY30 pKa = 9.19TQARR34 pKa = 11.84TLDD37 pKa = 3.64EE38 pKa = 4.8VGPMAADD45 pKa = 6.26LIATWLNVPVEE56 pKa = 4.4SVDD59 pKa = 4.08VEE61 pKa = 4.63VKK63 pKa = 9.71YY64 pKa = 9.62TDD66 pKa = 3.01

Molecular weight: 7.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z5HAK9|A0A2Z5HAK9_9CAUD Uncharacterized protein OS=Mycobacterium phage Biglebops OX=2250372 GN=44 PE=4 SV=1
MM1 pKa = 7.36QNFRR5 pKa = 11.84HH6 pKa = 5.31EE7 pKa = 4.53LRR9 pKa = 11.84WNSDD13 pKa = 2.77QVAKK17 pKa = 10.56VLRR20 pKa = 11.84ANGIHH25 pKa = 6.36GRR27 pKa = 11.84YY28 pKa = 8.69EE29 pKa = 3.69LSRR32 pKa = 11.84RR33 pKa = 11.84LNLTKK38 pKa = 10.51SVVYY42 pKa = 10.27VSFGPDD48 pKa = 2.49WSGTATHH55 pKa = 6.27NMVATLAAEE64 pKa = 4.38FGVPVGNLVVVAEE77 pKa = 4.51RR78 pKa = 11.84AAAA81 pKa = 3.67

Molecular weight: 8.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

77

0

77

17359

39

1377

225.4

24.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.754 ± 0.556

1.071 ± 0.145

6.475 ± 0.258

6.118 ± 0.324

2.633 ± 0.178

8.664 ± 0.55

2.189 ± 0.188

3.756 ± 0.185

3.209 ± 0.24

8.295 ± 0.218

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.091 ± 0.113

2.794 ± 0.243

5.565 ± 0.294

3.243 ± 0.179

7.529 ± 0.376

5.196 ± 0.217

5.588 ± 0.169

8.353 ± 0.349

2.166 ± 0.11

2.31 ± 0.196
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski