Amino acid dipepetide frequency for Mycobacterium phage Biglebops

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
22.007AlaAla: 22.007 ± 1.706
1.095AlaCys: 1.095 ± 0.266
8.584AlaAsp: 8.584 ± 0.89
9.218AlaGlu: 9.218 ± 1.025
2.996AlaPhe: 2.996 ± 0.646
8.181AlaGly: 8.181 ± 0.942
2.765AlaHis: 2.765 ± 0.374
4.724AlaIle: 4.724 ± 0.532
4.436AlaLys: 4.436 ± 0.561
11.061AlaLeu: 11.061 ± 0.861
3.399AlaMet: 3.399 ± 0.443
3.457AlaAsn: 3.457 ± 0.481
6.51AlaPro: 6.51 ± 0.758
4.666AlaGln: 4.666 ± 0.589
8.93AlaArg: 8.93 ± 0.764
4.782AlaSer: 4.782 ± 0.632
6.395AlaThr: 6.395 ± 0.784
9.275AlaVal: 9.275 ± 1.0
2.535AlaTrp: 2.535 ± 0.371
2.535AlaTyr: 2.535 ± 0.373
0.0AlaXaa: 0.0 ± 0.0
Cys
1.21CysAla: 1.21 ± 0.365
0.115CysCys: 0.115 ± 0.08
0.691CysAsp: 0.691 ± 0.214
0.922CysGlu: 0.922 ± 0.251
0.058CysPhe: 0.058 ± 0.067
1.267CysGly: 1.267 ± 0.328
0.173CysHis: 0.173 ± 0.102
0.403CysIle: 0.403 ± 0.152
0.23CysLys: 0.23 ± 0.131
0.461CysLeu: 0.461 ± 0.166
0.23CysMet: 0.23 ± 0.106
0.23CysAsn: 0.23 ± 0.108
0.922CysPro: 0.922 ± 0.237
0.288CysGln: 0.288 ± 0.135
0.922CysArg: 0.922 ± 0.21
0.979CysSer: 0.979 ± 0.302
0.23CysThr: 0.23 ± 0.119
0.691CysVal: 0.691 ± 0.181
0.518CysTrp: 0.518 ± 0.162
0.173CysTyr: 0.173 ± 0.097
0.0CysXaa: 0.0 ± 0.0
Asp
7.489AspAla: 7.489 ± 0.696
0.807AspCys: 0.807 ± 0.215
5.588AspAsp: 5.588 ± 0.757
6.337AspGlu: 6.337 ± 0.625
1.44AspPhe: 1.44 ± 0.231
5.934AspGly: 5.934 ± 0.616
0.979AspHis: 0.979 ± 0.269
1.037AspIle: 1.037 ± 0.279
2.132AspLys: 2.132 ± 0.316
6.107AspLeu: 6.107 ± 0.524
1.613AspMet: 1.613 ± 0.402
1.671AspAsn: 1.671 ± 0.299
4.033AspPro: 4.033 ± 0.447
2.132AspGln: 2.132 ± 0.395
4.378AspArg: 4.378 ± 0.515
2.535AspSer: 2.535 ± 0.394
2.708AspThr: 2.708 ± 0.431
5.243AspVal: 5.243 ± 0.462
1.21AspTrp: 1.21 ± 0.326
1.383AspTyr: 1.383 ± 0.321
0.0AspXaa: 0.0 ± 0.0
Glu
7.835GluAla: 7.835 ± 0.862
0.979GluCys: 0.979 ± 0.28
3.169GluAsp: 3.169 ± 0.554
1.671GluGlu: 1.671 ± 0.326
1.959GluPhe: 1.959 ± 0.296
4.897GluGly: 4.897 ± 0.515
1.671GluHis: 1.671 ± 0.382
2.132GluIle: 2.132 ± 0.388
1.498GluLys: 1.498 ± 0.359
6.683GluLeu: 6.683 ± 0.578
1.383GluMet: 1.383 ± 0.264
1.152GluAsn: 1.152 ± 0.265
3.284GluPro: 3.284 ± 0.474
2.65GluGln: 2.65 ± 0.405
5.358GluArg: 5.358 ± 0.629
2.823GluSer: 2.823 ± 0.45
2.881GluThr: 2.881 ± 0.396
5.991GluVal: 5.991 ± 0.885
1.095GluTrp: 1.095 ± 0.229
2.074GluTyr: 2.074 ± 0.37
0.0GluXaa: 0.0 ± 0.0
Phe
2.477PheAla: 2.477 ± 0.376
0.23PheCys: 0.23 ± 0.107
3.053PheAsp: 3.053 ± 0.38
1.844PheGlu: 1.844 ± 0.27
0.576PhePhe: 0.576 ± 0.167
3.226PheGly: 3.226 ± 0.518
0.576PheHis: 0.576 ± 0.225
0.749PheIle: 0.749 ± 0.183
0.979PheLys: 0.979 ± 0.271
1.959PheLeu: 1.959 ± 0.317
0.403PheMet: 0.403 ± 0.144
1.267PheAsn: 1.267 ± 0.43
0.922PhePro: 0.922 ± 0.227
0.749PheGln: 0.749 ± 0.192
1.498PheArg: 1.498 ± 0.273
0.864PheSer: 0.864 ± 0.275
1.44PheThr: 1.44 ± 0.354
2.42PheVal: 2.42 ± 0.374
0.461PheTrp: 0.461 ± 0.133
0.634PheTyr: 0.634 ± 0.239
0.0PheXaa: 0.0 ± 0.0
Gly
9.045GlyAla: 9.045 ± 1.097
0.807GlyCys: 0.807 ± 0.286
5.415GlyAsp: 5.415 ± 0.529
5.07GlyGlu: 5.07 ± 0.547
1.901GlyPhe: 1.901 ± 0.314
9.045GlyGly: 9.045 ± 1.338
1.555GlyHis: 1.555 ± 0.322
2.823GlyIle: 2.823 ± 0.748
3.284GlyLys: 3.284 ± 0.546
6.625GlyLeu: 6.625 ± 0.891
1.498GlyMet: 1.498 ± 0.304
3.053GlyAsn: 3.053 ± 0.479
3.86GlyPro: 3.86 ± 0.687
2.65GlyGln: 2.65 ± 0.368
6.222GlyArg: 6.222 ± 0.736
5.185GlySer: 5.185 ± 0.724
6.049GlyThr: 6.049 ± 0.606
7.547GlyVal: 7.547 ± 0.534
2.65GlyTrp: 2.65 ± 0.381
2.362GlyTyr: 2.362 ± 0.41
0.0GlyXaa: 0.0 ± 0.0
His
2.477HisAla: 2.477 ± 0.487
0.288HisCys: 0.288 ± 0.11
1.21HisAsp: 1.21 ± 0.304
1.325HisGlu: 1.325 ± 0.282
0.634HisPhe: 0.634 ± 0.173
2.304HisGly: 2.304 ± 0.412
0.576HisHis: 0.576 ± 0.199
0.922HisIle: 0.922 ± 0.24
0.346HisLys: 0.346 ± 0.155
1.959HisLeu: 1.959 ± 0.297
0.576HisMet: 0.576 ± 0.201
0.634HisAsn: 0.634 ± 0.191
1.383HisPro: 1.383 ± 0.261
0.518HisGln: 0.518 ± 0.176
1.498HisArg: 1.498 ± 0.455
0.691HisSer: 0.691 ± 0.192
1.44HisThr: 1.44 ± 0.27
2.016HisVal: 2.016 ± 0.407
0.403HisTrp: 0.403 ± 0.177
0.691HisTyr: 0.691 ± 0.269
0.0HisXaa: 0.0 ± 0.0
Ile
5.415IleAla: 5.415 ± 0.542
0.173IleCys: 0.173 ± 0.119
2.938IleAsp: 2.938 ± 0.345
2.996IleGlu: 2.996 ± 0.59
0.922IlePhe: 0.922 ± 0.246
3.341IleGly: 3.341 ± 0.728
0.518IleHis: 0.518 ± 0.215
0.807IleIle: 0.807 ± 0.208
1.613IleLys: 1.613 ± 0.32
2.938IleLeu: 2.938 ± 0.423
0.403IleMet: 0.403 ± 0.139
1.21IleAsn: 1.21 ± 0.217
2.362IlePro: 2.362 ± 0.437
0.634IleGln: 0.634 ± 0.243
2.592IleArg: 2.592 ± 0.328
1.44IleSer: 1.44 ± 0.264
2.535IleThr: 2.535 ± 0.345
3.341IleVal: 3.341 ± 0.452
0.864IleTrp: 0.864 ± 0.24
0.518IleTyr: 0.518 ± 0.171
0.0IleXaa: 0.0 ± 0.0
Lys
4.436LysAla: 4.436 ± 0.631
0.461LysCys: 0.461 ± 0.163
1.095LysAsp: 1.095 ± 0.262
0.864LysGlu: 0.864 ± 0.241
1.498LysPhe: 1.498 ± 0.408
3.053LysGly: 3.053 ± 0.507
0.634LysHis: 0.634 ± 0.208
1.671LysIle: 1.671 ± 0.392
0.807LysLys: 0.807 ± 0.257
2.938LysLeu: 2.938 ± 0.362
0.691LysMet: 0.691 ± 0.215
0.518LysAsn: 0.518 ± 0.18
2.304LysPro: 2.304 ± 0.419
0.634LysGln: 0.634 ± 0.197
3.169LysArg: 3.169 ± 0.531
1.613LysSer: 1.613 ± 0.303
1.671LysThr: 1.671 ± 0.319
2.823LysVal: 2.823 ± 0.452
0.403LysTrp: 0.403 ± 0.126
0.807LysTyr: 0.807 ± 0.259
0.0LysXaa: 0.0 ± 0.0
Leu
12.213LeuAla: 12.213 ± 0.823
0.634LeuCys: 0.634 ± 0.217
7.374LeuAsp: 7.374 ± 0.741
3.111LeuGlu: 3.111 ± 0.429
2.132LeuPhe: 2.132 ± 0.379
6.395LeuGly: 6.395 ± 0.735
1.959LeuHis: 1.959 ± 0.324
3.514LeuIle: 3.514 ± 0.39
2.708LeuLys: 2.708 ± 0.449
5.07LeuLeu: 5.07 ± 0.58
1.498LeuMet: 1.498 ± 0.291
2.362LeuAsn: 2.362 ± 0.321
4.148LeuPro: 4.148 ± 0.469
3.169LeuGln: 3.169 ± 0.554
7.144LeuArg: 7.144 ± 0.802
5.934LeuSer: 5.934 ± 0.598
5.07LeuThr: 5.07 ± 0.588
5.473LeuVal: 5.473 ± 0.617
1.267LeuTrp: 1.267 ± 0.398
1.786LeuTyr: 1.786 ± 0.397
0.0LeuXaa: 0.0 ± 0.0
Met
2.477MetAla: 2.477 ± 0.289
0.173MetCys: 0.173 ± 0.112
0.864MetAsp: 0.864 ± 0.215
0.576MetGlu: 0.576 ± 0.217
0.922MetPhe: 0.922 ± 0.239
1.21MetGly: 1.21 ± 0.266
0.403MetHis: 0.403 ± 0.182
1.037MetIle: 1.037 ± 0.29
0.634MetLys: 0.634 ± 0.175
1.383MetLeu: 1.383 ± 0.314
0.288MetMet: 0.288 ± 0.124
0.576MetAsn: 0.576 ± 0.177
1.21MetPro: 1.21 ± 0.321
0.634MetGln: 0.634 ± 0.195
1.498MetArg: 1.498 ± 0.335
2.42MetSer: 2.42 ± 0.294
1.959MetThr: 1.959 ± 0.36
1.555MetVal: 1.555 ± 0.337
0.403MetTrp: 0.403 ± 0.135
0.691MetTyr: 0.691 ± 0.227
0.0MetXaa: 0.0 ± 0.0
Asn
4.09AsnAla: 4.09 ± 0.807
0.518AsnCys: 0.518 ± 0.177
1.095AsnAsp: 1.095 ± 0.247
1.21AsnGlu: 1.21 ± 0.256
0.634AsnPhe: 0.634 ± 0.175
3.572AsnGly: 3.572 ± 0.606
0.461AsnHis: 0.461 ± 0.175
0.807AsnIle: 0.807 ± 0.294
1.152AsnLys: 1.152 ± 0.276
2.189AsnLeu: 2.189 ± 0.39
0.749AsnMet: 0.749 ± 0.169
0.807AsnAsn: 0.807 ± 0.316
2.708AsnPro: 2.708 ± 0.393
0.576AsnGln: 0.576 ± 0.188
1.498AsnArg: 1.498 ± 0.279
1.44AsnSer: 1.44 ± 0.301
1.44AsnThr: 1.44 ± 0.331
2.074AsnVal: 2.074 ± 0.311
0.403AsnTrp: 0.403 ± 0.158
0.518AsnTyr: 0.518 ± 0.174
0.0AsnXaa: 0.0 ± 0.0
Pro
7.317ProAla: 7.317 ± 0.628
0.518ProCys: 0.518 ± 0.191
3.169ProAsp: 3.169 ± 0.593
4.782ProGlu: 4.782 ± 0.608
1.383ProPhe: 1.383 ± 0.29
5.876ProGly: 5.876 ± 0.548
1.267ProHis: 1.267 ± 0.257
2.304ProIle: 2.304 ± 0.329
1.671ProLys: 1.671 ± 0.289
4.148ProLeu: 4.148 ± 0.574
0.979ProMet: 0.979 ± 0.197
1.037ProAsn: 1.037 ± 0.347
2.996ProPro: 2.996 ± 0.428
1.21ProGln: 1.21 ± 0.359
3.284ProArg: 3.284 ± 0.454
2.823ProSer: 2.823 ± 0.454
2.823ProThr: 2.823 ± 0.424
5.588ProVal: 5.588 ± 0.798
1.21ProTrp: 1.21 ± 0.255
1.267ProTyr: 1.267 ± 0.275
0.0ProXaa: 0.0 ± 0.0
Gln
4.09GlnAla: 4.09 ± 0.592
0.173GlnCys: 0.173 ± 0.098
1.21GlnAsp: 1.21 ± 0.276
1.498GlnGlu: 1.498 ± 0.271
0.807GlnPhe: 0.807 ± 0.266
2.362GlnGly: 2.362 ± 0.316
1.267GlnHis: 1.267 ± 0.233
1.728GlnIle: 1.728 ± 0.308
0.807GlnLys: 0.807 ± 0.228
3.053GlnLeu: 3.053 ± 0.402
0.576GlnMet: 0.576 ± 0.17
0.691GlnAsn: 0.691 ± 0.293
2.189GlnPro: 2.189 ± 0.377
1.44GlnGln: 1.44 ± 0.254
2.765GlnArg: 2.765 ± 0.376
1.44GlnSer: 1.44 ± 0.343
1.728GlnThr: 1.728 ± 0.249
3.053GlnVal: 3.053 ± 0.446
0.461GlnTrp: 0.461 ± 0.185
1.095GlnTyr: 1.095 ± 0.201
0.0GlnXaa: 0.0 ± 0.0
Arg
7.317ArgAla: 7.317 ± 0.959
1.037ArgCys: 1.037 ± 0.285
4.033ArgAsp: 4.033 ± 0.416
4.551ArgGlu: 4.551 ± 0.653
2.535ArgPhe: 2.535 ± 0.423
5.127ArgGly: 5.127 ± 0.644
2.189ArgHis: 2.189 ± 0.451
3.514ArgIle: 3.514 ± 0.478
2.938ArgLys: 2.938 ± 0.506
6.568ArgLeu: 6.568 ± 0.736
2.477ArgMet: 2.477 ± 0.316
2.477ArgAsn: 2.477 ± 0.412
3.457ArgPro: 3.457 ± 0.57
2.881ArgGln: 2.881 ± 0.419
6.452ArgArg: 6.452 ± 0.746
4.321ArgSer: 4.321 ± 0.668
3.918ArgThr: 3.918 ± 0.479
5.819ArgVal: 5.819 ± 0.666
2.247ArgTrp: 2.247 ± 0.336
1.44ArgTyr: 1.44 ± 0.243
0.0ArgXaa: 0.0 ± 0.0
Ser
6.452SerAla: 6.452 ± 0.63
0.518SerCys: 0.518 ± 0.212
3.341SerAsp: 3.341 ± 0.428
2.535SerGlu: 2.535 ± 0.43
1.44SerPhe: 1.44 ± 0.248
5.243SerGly: 5.243 ± 0.757
1.037SerHis: 1.037 ± 0.32
2.074SerIle: 2.074 ± 0.4
1.21SerLys: 1.21 ± 0.271
4.321SerLeu: 4.321 ± 0.441
0.979SerMet: 0.979 ± 0.259
1.44SerAsn: 1.44 ± 0.274
2.938SerPro: 2.938 ± 0.4
1.613SerGln: 1.613 ± 0.261
4.206SerArg: 4.206 ± 0.512
3.341SerSer: 3.341 ± 0.494
2.65SerThr: 2.65 ± 0.382
4.263SerVal: 4.263 ± 0.503
1.267SerTrp: 1.267 ± 0.222
1.095SerTyr: 1.095 ± 0.251
0.0SerXaa: 0.0 ± 0.0
Thr
6.913ThrAla: 6.913 ± 0.72
0.461ThrCys: 0.461 ± 0.189
3.053ThrAsp: 3.053 ± 0.422
4.033ThrGlu: 4.033 ± 0.45
1.901ThrPhe: 1.901 ± 0.352
5.3ThrGly: 5.3 ± 0.594
1.555ThrHis: 1.555 ± 0.337
2.996ThrIle: 2.996 ± 0.402
1.901ThrLys: 1.901 ± 0.345
4.321ThrLeu: 4.321 ± 0.532
0.576ThrMet: 0.576 ± 0.185
1.498ThrAsn: 1.498 ± 0.279
3.284ThrPro: 3.284 ± 0.495
1.44ThrGln: 1.44 ± 0.315
3.687ThrArg: 3.687 ± 0.616
2.938ThrSer: 2.938 ± 0.557
2.708ThrThr: 2.708 ± 0.458
4.897ThrVal: 4.897 ± 0.481
1.21ThrTrp: 1.21 ± 0.227
1.21ThrTyr: 1.21 ± 0.233
0.0ThrXaa: 0.0 ± 0.0
Val
9.909ValAla: 9.909 ± 0.802
0.979ValCys: 0.979 ± 0.232
6.28ValAsp: 6.28 ± 0.52
6.971ValGlu: 6.971 ± 0.841
1.498ValPhe: 1.498 ± 0.384
6.28ValGly: 6.28 ± 0.682
1.671ValHis: 1.671 ± 0.434
3.053ValIle: 3.053 ± 0.398
2.535ValLys: 2.535 ± 0.406
6.568ValLeu: 6.568 ± 0.814
1.728ValMet: 1.728 ± 0.3
2.189ValAsn: 2.189 ± 0.381
5.531ValPro: 5.531 ± 0.63
2.189ValGln: 2.189 ± 0.359
5.473ValArg: 5.473 ± 0.717
3.572ValSer: 3.572 ± 0.52
5.703ValThr: 5.703 ± 0.602
7.547ValVal: 7.547 ± 0.911
1.959ValTrp: 1.959 ± 0.339
1.901ValTyr: 1.901 ± 0.284
0.0ValXaa: 0.0 ± 0.0
Trp
2.132TrpAla: 2.132 ± 0.39
0.346TrpCys: 0.346 ± 0.142
0.979TrpAsp: 0.979 ± 0.303
0.634TrpGlu: 0.634 ± 0.23
0.576TrpPhe: 0.576 ± 0.177
1.498TrpGly: 1.498 ± 0.352
0.403TrpHis: 0.403 ± 0.14
0.576TrpIle: 0.576 ± 0.172
0.403TrpLys: 0.403 ± 0.136
3.169TrpLeu: 3.169 ± 0.46
0.288TrpMet: 0.288 ± 0.148
0.864TrpAsn: 0.864 ± 0.277
0.807TrpPro: 0.807 ± 0.215
1.44TrpGln: 1.44 ± 0.33
2.477TrpArg: 2.477 ± 0.434
1.44TrpSer: 1.44 ± 0.325
0.807TrpThr: 0.807 ± 0.252
1.786TrpVal: 1.786 ± 0.384
0.518TrpTrp: 0.518 ± 0.164
0.461TrpTyr: 0.461 ± 0.166
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.247TyrAla: 2.247 ± 0.358
0.403TyrCys: 0.403 ± 0.148
1.613TyrAsp: 1.613 ± 0.32
1.037TyrGlu: 1.037 ± 0.276
0.518TyrPhe: 0.518 ± 0.196
2.535TyrGly: 2.535 ± 0.383
0.23TyrHis: 0.23 ± 0.122
0.691TyrIle: 0.691 ± 0.204
0.807TyrLys: 0.807 ± 0.239
1.44TyrLeu: 1.44 ± 0.349
0.518TyrMet: 0.518 ± 0.167
0.807TyrAsn: 0.807 ± 0.174
0.807TyrPro: 0.807 ± 0.238
0.922TyrGln: 0.922 ± 0.258
2.247TyrArg: 2.247 ± 0.354
1.383TyrSer: 1.383 ± 0.305
1.728TyrThr: 1.728 ± 0.366
2.132TyrVal: 2.132 ± 0.387
0.576TyrTrp: 0.576 ± 0.172
0.461TyrTyr: 0.461 ± 0.169
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 77 proteins (17359 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski