Chaetothyriales sp. CBS 132003
Average proteome isoelectric point is 6.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5726 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3M7MVX4|A0A3M7MVX4_9EURO WD_REPEATS_REGION domain-containing protein (Fragment) OS=Chaetothyriales sp. CBS 132003 OX=2249419 GN=DV737_g4098 PE=4 SV=1
MM1 pKa = 7.51 LFSSYY6 pKa = 10.7 AVQASVLLLISTVFGQYY23 pKa = 10.44 SLQDD27 pKa = 3.87 DD28 pKa = 4.57 YY29 pKa = 10.14 TTSSFGSMFDD39 pKa = 4.33 FFTDD43 pKa = 3.8 DD44 pKa = 4.91 DD45 pKa = 4.15 PTSGYY50 pKa = 10.55 VNYY53 pKa = 10.01 VSQSEE58 pKa = 4.53 AEE60 pKa = 4.28 SQGLFTTEE68 pKa = 3.7 NNTVYY73 pKa = 10.43 IGVDD77 pKa = 3.42 STNVASGRR85 pKa = 11.84 GRR87 pKa = 11.84 NSVRR91 pKa = 11.84 ISSKK95 pKa = 10.3 NVYY98 pKa = 7.72 THH100 pKa = 6.5 GLIILDD106 pKa = 4.2 LEE108 pKa = 4.47 HH109 pKa = 6.91 MPGGQCGSWPAFWTLGPDD127 pKa = 2.86 WPTWGEE133 pKa = 3.1 IDD135 pKa = 4.36 IIEE138 pKa = 4.51 NVNSATVNTISAHH151 pKa = 5.53 TDD153 pKa = 3.16 SSCSISATGNFTGSILTTDD172 pKa = 4.54 CDD174 pKa = 3.56 SSAASEE180 pKa = 4.42 SSDD183 pKa = 3.51 SGCSFTDD190 pKa = 3.11 TSTQTFGAGFNAIGGGVYY208 pKa = 8.89 ATEE211 pKa = 4.26 WTSEE215 pKa = 4.31 AISIWFFPRR224 pKa = 11.84 LLIPSDD230 pKa = 3.41 ITNGSPDD237 pKa = 3.39 PSGWGEE243 pKa = 3.87 PLAQFTGGGCDD254 pKa = 3.79 IDD256 pKa = 5.01 DD257 pKa = 3.9 HH258 pKa = 6.65 FKK260 pKa = 11.11 NNQVVFDD267 pKa = 4.64 ISMCGDD273 pKa = 2.98 WAGDD277 pKa = 3.41 VWSQDD282 pKa = 3.28 ATCSLLASTCEE293 pKa = 5.14 DD294 pKa = 3.69 YY295 pKa = 11.48 VQNNPDD301 pKa = 4.0 AFVDD305 pKa = 4.35 TYY307 pKa = 11.35 WLINSLKK314 pKa = 10.38 VYY316 pKa = 10.28 QEE318 pKa = 4.2 NNQQDD323 pKa = 4.22 TTSSLSPSSTAGTTTSTASSAATITTTSSSVVPTTFEE360 pKa = 3.86 TSTRR364 pKa = 11.84 PIVVPSSVTSTDD376 pKa = 2.5 TWSASVSIPTSTDD389 pKa = 1.95 IWSASVSIPTSTDD402 pKa = 2.83 APSVLEE408 pKa = 4.25 STSTTTDD415 pKa = 2.98 TSSVSVAGSPFATQGWQDD433 pKa = 3.7 LDD435 pKa = 3.68 GGKK438 pKa = 9.83 QGWGLQGSGWGQRR451 pKa = 11.84 NRR453 pKa = 11.84 GRR455 pKa = 11.84 WSGGDD460 pKa = 3.1
Molecular weight: 48.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.76
IPC2_protein 3.656
IPC_protein 3.694
Toseland 3.452
ProMoST 3.846
Dawson 3.694
Bjellqvist 3.872
Wikipedia 3.656
Rodwell 3.516
Grimsley 3.363
Solomon 3.681
Lehninger 3.643
Nozaki 3.808
DTASelect 4.088
Thurlkill 3.516
EMBOSS 3.656
Sillero 3.808
Patrickios 0.451
IPC_peptide 3.681
IPC2_peptide 3.783
IPC2.peptide.svr19 3.755
Protein with the highest isoelectric point:
>tr|A0A3M7MQD0|A0A3M7MQD0_9EURO PCI domain-containing protein (Fragment) OS=Chaetothyriales sp. CBS 132003 OX=2249419 GN=DV737_g4633 PE=4 SV=1
MM1 pKa = 7.96 PSTADD6 pKa = 3.45 GDD8 pKa = 4.32 CSQTHH13 pKa = 6.0 AQTHH17 pKa = 5.2 AQTRR21 pKa = 11.84 VQTRR25 pKa = 11.84 TSAASPNDD33 pKa = 4.06 SIRR36 pKa = 11.84 PPAPARR42 pKa = 11.84 CSRR45 pKa = 11.84 MVSRR49 pKa = 11.84 CGAAEE54 pKa = 3.88 RR55 pKa = 11.84 TRR57 pKa = 11.84 LQDD60 pKa = 3.26 VVQQRR65 pKa = 11.84 IARR68 pKa = 11.84 LPHH71 pKa = 5.52 AQQLARR77 pKa = 11.84 LHH79 pKa = 6.44 PKK81 pKa = 9.44 RR82 pKa = 11.84 AAARR86 pKa = 11.84 TSSANASQPRR96 pKa = 11.84 TQTPAEE102 pKa = 4.38 VKK104 pKa = 9.76 SQRR107 pKa = 11.84 PEE109 pKa = 3.34 ARR111 pKa = 11.84 QTGRR115 pKa = 11.84 SPRR118 pKa = 11.84 EE119 pKa = 3.39 EE120 pKa = 3.7 WQKK123 pKa = 10.76 FIHH126 pKa = 6.54 RR127 pKa = 11.84 LSALPRR133 pKa = 11.84 TRR135 pKa = 11.84 PSRR138 pKa = 11.84 EE139 pKa = 3.83 SNGRR143 pKa = 11.84 CVEE146 pKa = 3.92 LTTAFVHH153 pKa = 6.76 HH154 pKa = 5.76 YY155 pKa = 9.41 TGTARR160 pKa = 11.84 PNSWIQDD167 pKa = 3.49 VGSRR171 pKa = 11.84 AVVEE175 pKa = 4.84 LLPSSPPEE183 pKa = 3.48 RR184 pKa = 11.84 HH185 pKa = 5.68 LRR187 pKa = 11.84 QAILHH192 pKa = 5.75 GSDD195 pKa = 3.51 RR196 pKa = 11.84 AVEE199 pKa = 3.91 LAKK202 pKa = 10.64 LDD204 pKa = 4.01 LAKK207 pKa = 10.66 LQAEE211 pKa = 4.86 FEE213 pKa = 4.76 SSGHH217 pKa = 6.29 PALAAHH223 pKa = 6.47 PTDD226 pKa = 3.63 VSLDD230 pKa = 3.93 TIDD233 pKa = 4.01 SVSSFARR240 pKa = 11.84 FVLQTTNRR248 pKa = 11.84 YY249 pKa = 8.86 IPPALQRR256 pKa = 11.84 DD257 pKa = 3.97 SGRR260 pKa = 11.84 SFLDD264 pKa = 3.39 SVSDD268 pKa = 3.41 ALTAVFEE275 pKa = 4.5 HH276 pKa = 6.65 PVVCRR281 pKa = 11.84 FASTVALNDD290 pKa = 3.56 ALRR293 pKa = 11.84 FLCRR297 pKa = 11.84 RR298 pKa = 11.84 NHH300 pKa = 6.91 AEE302 pKa = 3.89 AADD305 pKa = 3.76 AAHH308 pKa = 7.14 RR309 pKa = 11.84 LYY311 pKa = 10.75 HH312 pKa = 6.12 AAKK315 pKa = 10.17 ALHH318 pKa = 6.29 LVLGISTFNILFDD331 pKa = 3.64 SAIRR335 pKa = 11.84 TGNAKK340 pKa = 10.02 RR341 pKa = 11.84 AVFFLRR347 pKa = 11.84 EE348 pKa = 4.0 MQRR351 pKa = 11.84 EE352 pKa = 4.42 SICPNSHH359 pKa = 4.79 TWTLLFSIPSISDD372 pKa = 2.88 KK373 pKa = 10.78 TALLRR378 pKa = 11.84 LIMEE382 pKa = 4.78 SGIPMNRR389 pKa = 11.84 QGFAIITRR397 pKa = 11.84 AAIADD402 pKa = 4.36 LLPNQRR408 pKa = 11.84 PSCEE412 pKa = 4.32 DD413 pKa = 3.34 LQQLFADD420 pKa = 4.73 LDD422 pKa = 4.51 HH423 pKa = 7.25 IFGPDD428 pKa = 2.93 WLTVDD433 pKa = 4.57 SYY435 pKa = 11.99 HH436 pKa = 7.21 LLLRR440 pKa = 11.84 LSSLNPRR447 pKa = 11.84 EE448 pKa = 4.2 GVVGLLNEE456 pKa = 4.24 LPSRR460 pKa = 11.84 HH461 pKa = 5.57 GLKK464 pKa = 10.17 LAPDD468 pKa = 3.82 SVRR471 pKa = 11.84 YY472 pKa = 8.7 LAALNRR478 pKa = 11.84 KK479 pKa = 8.47 SAITRR484 pKa = 11.84 DD485 pKa = 3.68 RR486 pKa = 11.84 ISDD489 pKa = 3.66 FVAAVGAPTCARR501 pKa = 11.84 QRR503 pKa = 11.84 LVVPRR508 pKa = 11.84 FFMAAWRR515 pKa = 11.84 RR516 pKa = 11.84 CFYY519 pKa = 10.65 NVCAVLWSFAATRR532 pKa = 11.84 GLITQTMLRR541 pKa = 11.84 IVSRR545 pKa = 11.84 YY546 pKa = 7.93 LVRR549 pKa = 11.84 GGAHH553 pKa = 6.57 PSSRR557 pKa = 11.84 SASRR561 pKa = 11.84 KK562 pKa = 9.46 RR563 pKa = 11.84 IGARR567 pKa = 11.84 IVAGVQWRR575 pKa = 11.84 RR576 pKa = 11.84 GPHH579 pKa = 4.68 QVAVMADD586 pKa = 3.76 VFPKK590 pKa = 9.76 LARR593 pKa = 11.84 RR594 pKa = 11.84 FGEE597 pKa = 4.07 HH598 pKa = 5.17 NNARR602 pKa = 11.84 RR603 pKa = 11.84 WLTGFTADD611 pKa = 4.87 DD612 pKa = 3.89 GTRR615 pKa = 11.84 DD616 pKa = 3.28 EE617 pKa = 4.33 QMNLFFTTLHH627 pKa = 7.28 DD628 pKa = 6.39 DD629 pKa = 3.56 LGAWKK634 pKa = 10.19 RR635 pKa = 11.84 FRR637 pKa = 11.84 PMSYY641 pKa = 10.96 GSLQRR646 pKa = 11.84 MLDD649 pKa = 3.25 QAYY652 pKa = 9.41 EE653 pKa = 3.94 ADD655 pKa = 3.34 QRR657 pKa = 11.84 SISEE661 pKa = 4.23 GTLDD665 pKa = 3.57 AQGEE669 pKa = 4.54 HH670 pKa = 6.56 AVSQAVDD677 pKa = 3.33 RR678 pKa = 11.84 AICVPLTKK686 pKa = 10.56 LVDD689 pKa = 4.16 GEE691 pKa = 4.8 ANWAVQAKK699 pKa = 9.3 INQRR703 pKa = 11.84 DD704 pKa = 3.38 RR705 pKa = 11.84 VMDD708 pKa = 4.5 AARR711 pKa = 11.84 LSRR714 pKa = 11.84 RR715 pKa = 11.84 RR716 pKa = 11.84 GPASGTLHH724 pKa = 7.56 DD725 pKa = 4.85 LRR727 pKa = 6.49
Molecular weight: 81.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.35
IPC2_protein 9.414
IPC_protein 10.438
Toseland 10.599
ProMoST 10.526
Dawson 10.687
Bjellqvist 10.467
Wikipedia 10.95
Rodwell 10.672
Grimsley 10.73
Solomon 10.862
Lehninger 10.818
Nozaki 10.613
DTASelect 10.452
Thurlkill 10.599
EMBOSS 11.023
Sillero 10.643
Patrickios 10.335
IPC_peptide 10.877
IPC2_peptide 9.809
IPC2.peptide.svr19 8.721
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5726
0
5726
3461870
48
5823
604.6
66.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.704 ± 0.028
1.065 ± 0.009
5.979 ± 0.021
5.86 ± 0.021
3.552 ± 0.018
6.723 ± 0.023
2.517 ± 0.013
4.695 ± 0.019
4.814 ± 0.022
9.142 ± 0.026
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.987 ± 0.011
3.41 ± 0.015
5.927 ± 0.028
4.2 ± 0.019
6.264 ± 0.021
8.13 ± 0.029
5.783 ± 0.018
6.21 ± 0.019
1.365 ± 0.009
2.674 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here