Streptomyces phage Shawty

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Lomovskayavirus; unclassified Lomovskayavirus

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A411CYG7|A0A411CYG7_9CAUD Uncharacterized protein OS=Streptomyces phage Shawty OX=2510521 GN=29 PE=4 SV=1
MM1 pKa = 7.22TALPNEE7 pKa = 4.32IPTVRR12 pKa = 11.84VTGTYY17 pKa = 10.06RR18 pKa = 11.84GPDD21 pKa = 3.06GRR23 pKa = 11.84ALKK26 pKa = 9.34GTVTFTGPPLLTFSEE41 pKa = 4.41SDD43 pKa = 3.52LFIAGPVVATLDD55 pKa = 3.56EE56 pKa = 4.77AGQIIDD62 pKa = 3.43ADD64 pKa = 4.18GNIGVHH70 pKa = 6.66LPATDD75 pKa = 3.94APNMNPTGWLWTVKK89 pKa = 10.49EE90 pKa = 4.13NLTGVTGARR99 pKa = 11.84TYY101 pKa = 11.91SMVLPKK107 pKa = 9.97DD108 pKa = 3.58TLNNTVDD115 pKa = 3.97LADD118 pKa = 3.71VAPANPATPNYY129 pKa = 9.37VAVPGPSAYY138 pKa = 9.46EE139 pKa = 3.73VAVAEE144 pKa = 4.63GFTGTEE150 pKa = 4.13AEE152 pKa = 4.39WLDD155 pKa = 4.1SLEE158 pKa = 4.68GPQGPQGIQGVKK170 pKa = 10.29GDD172 pKa = 4.35TGAQGPKK179 pKa = 9.84GDD181 pKa = 4.55KK182 pKa = 10.91GDD184 pKa = 3.92TGDD187 pKa = 4.08VGPTGPQGEE196 pKa = 4.71KK197 pKa = 10.9GDD199 pKa = 5.0DD200 pKa = 3.42GDD202 pKa = 3.73SAYY205 pKa = 10.22EE206 pKa = 3.81VAVANGFVGTEE217 pKa = 4.12AEE219 pKa = 4.27WLASLIGPEE228 pKa = 4.57GPEE231 pKa = 4.61GPQGPAGADD240 pKa = 3.32GTGAGTVTAVNGVEE254 pKa = 3.91PDD256 pKa = 3.26ANGNVTLTLTAEE268 pKa = 4.2AVGAIPVADD277 pKa = 3.8KK278 pKa = 11.25GVASGVATLGTDD290 pKa = 3.58GKK292 pKa = 10.89VPSAQLPTLADD303 pKa = 3.92PNAVTSVNGKK313 pKa = 9.57SGPTVTLTAADD324 pKa = 3.93VSALASSLRR333 pKa = 11.84GAANGVASLDD343 pKa = 3.72SAKK346 pKa = 10.26VVPVAQLPGSGFLPTDD362 pKa = 3.78LGMKK366 pKa = 9.87AWAFDD371 pKa = 3.74PATSQTGGRR380 pKa = 11.84APSSRR385 pKa = 11.84SFRR388 pKa = 11.84IVALPVRR395 pKa = 11.84QTMTVSKK402 pKa = 10.19FVFHH406 pKa = 5.9VLGYY410 pKa = 10.3EE411 pKa = 4.29GTGLDD416 pKa = 3.04SGSYY420 pKa = 9.27VRR422 pKa = 11.84LYY424 pKa = 10.66DD425 pKa = 3.56SSGAQVATTANMTDD439 pKa = 3.67TNVMTDD445 pKa = 2.3IHH447 pKa = 6.31NAGGQTVTCAMSSSVTLSPGVYY469 pKa = 8.96YY470 pKa = 10.87VGFYY474 pKa = 9.76FVIGNSANAPVLMTADD490 pKa = 3.43STAACPVTTLNSVKK504 pKa = 10.12PFGVISGLSSAPSSFSPSAVEE525 pKa = 3.57TDD527 pKa = 5.07PIRR530 pKa = 11.84FWAALTT536 pKa = 3.77

Molecular weight: 53.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A411CYJ4|A0A411CYJ4_9CAUD Uncharacterized protein OS=Streptomyces phage Shawty OX=2510521 GN=36 PE=4 SV=1
MM1 pKa = 6.24TTAARR6 pKa = 11.84IVEE9 pKa = 4.2QALKK13 pKa = 10.28ALEE16 pKa = 3.99IGRR19 pKa = 11.84TVIVVTNEE27 pKa = 3.42TKK29 pKa = 10.74AVLNTEE35 pKa = 4.02KK36 pKa = 10.73GRR38 pKa = 11.84VVGFVKK44 pKa = 10.52SSEE47 pKa = 3.95PGNWRR52 pKa = 11.84TAEE55 pKa = 3.68RR56 pKa = 11.84RR57 pKa = 11.84RR58 pKa = 11.84QEE60 pKa = 4.16EE61 pKa = 3.57IRR63 pKa = 11.84RR64 pKa = 11.84ALDD67 pKa = 3.21ADD69 pKa = 3.75RR70 pKa = 11.84VRR72 pKa = 11.84RR73 pKa = 11.84YY74 pKa = 9.76

Molecular weight: 8.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

12301

54

818

219.7

23.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.601 ± 0.439

0.984 ± 0.169

6.877 ± 0.309

5.967 ± 0.299

3.146 ± 0.191

8.739 ± 0.407

1.837 ± 0.159

3.536 ± 0.264

4.422 ± 0.327

7.837 ± 0.273

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.097 ± 0.125

2.902 ± 0.174

5.211 ± 0.353

2.374 ± 0.124

6.869 ± 0.398

5.447 ± 0.273

6.56 ± 0.351

8.081 ± 0.33

1.845 ± 0.155

2.666 ± 0.212
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski