Amino acid dipepetide frequency for Streptomyces phage Shawty

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.691AlaAla: 15.691 ± 1.347
1.057AlaCys: 1.057 ± 0.306
11.301AlaAsp: 11.301 ± 1.0
9.837AlaGlu: 9.837 ± 1.124
3.171AlaPhe: 3.171 ± 0.538
8.293AlaGly: 8.293 ± 0.915
2.764AlaHis: 2.764 ± 0.402
4.39AlaIle: 4.39 ± 0.612
5.61AlaLys: 5.61 ± 0.876
11.463AlaLeu: 11.463 ± 0.926
3.496AlaMet: 3.496 ± 0.434
3.577AlaAsn: 3.577 ± 0.574
6.016AlaPro: 6.016 ± 0.915
4.797AlaGln: 4.797 ± 0.619
6.423AlaArg: 6.423 ± 0.715
5.691AlaSer: 5.691 ± 0.786
6.911AlaThr: 6.911 ± 0.682
10.244AlaVal: 10.244 ± 0.942
1.951AlaTrp: 1.951 ± 0.395
3.333AlaTyr: 3.333 ± 0.491
0.0AlaXaa: 0.0 ± 0.0
Cys
1.057CysAla: 1.057 ± 0.341
0.081CysCys: 0.081 ± 0.091
0.813CysAsp: 0.813 ± 0.263
0.65CysGlu: 0.65 ± 0.201
0.081CysPhe: 0.081 ± 0.063
0.976CysGly: 0.976 ± 0.261
0.163CysHis: 0.163 ± 0.112
0.081CysIle: 0.081 ± 0.091
0.325CysLys: 0.325 ± 0.144
0.325CysLeu: 0.325 ± 0.228
0.081CysMet: 0.081 ± 0.08
0.488CysAsn: 0.488 ± 0.238
1.138CysPro: 1.138 ± 0.313
0.163CysGln: 0.163 ± 0.104
0.976CysArg: 0.976 ± 0.288
0.894CysSer: 0.894 ± 0.301
0.407CysThr: 0.407 ± 0.199
0.894CysVal: 0.894 ± 0.331
0.081CysTrp: 0.081 ± 0.086
0.163CysTyr: 0.163 ± 0.123
0.0CysXaa: 0.0 ± 0.0
Asp
10.0AspAla: 10.0 ± 0.899
0.569AspCys: 0.569 ± 0.207
5.285AspAsp: 5.285 ± 0.848
3.902AspGlu: 3.902 ± 0.6
2.033AspPhe: 2.033 ± 0.39
7.398AspGly: 7.398 ± 0.674
1.138AspHis: 1.138 ± 0.36
1.382AspIle: 1.382 ± 0.249
2.033AspLys: 2.033 ± 0.443
5.041AspLeu: 5.041 ± 0.603
1.789AspMet: 1.789 ± 0.43
1.789AspAsn: 1.789 ± 0.364
4.146AspPro: 4.146 ± 0.751
0.894AspGln: 0.894 ± 0.252
3.74AspArg: 3.74 ± 0.589
2.683AspSer: 2.683 ± 0.585
4.472AspThr: 4.472 ± 0.463
6.911AspVal: 6.911 ± 0.955
1.789AspTrp: 1.789 ± 0.333
1.789AspTyr: 1.789 ± 0.364
0.0AspXaa: 0.0 ± 0.0
Glu
9.024GluAla: 9.024 ± 0.896
0.325GluCys: 0.325 ± 0.166
2.439GluAsp: 2.439 ± 0.404
3.171GluGlu: 3.171 ± 0.537
2.276GluPhe: 2.276 ± 0.4
4.553GluGly: 4.553 ± 0.73
0.976GluHis: 0.976 ± 0.329
2.764GluIle: 2.764 ± 0.534
2.52GluLys: 2.52 ± 0.467
6.098GluLeu: 6.098 ± 1.113
0.488GluMet: 0.488 ± 0.211
1.382GluAsn: 1.382 ± 0.376
3.333GluPro: 3.333 ± 0.965
0.732GluGln: 0.732 ± 0.245
4.715GluArg: 4.715 ± 0.606
3.496GluSer: 3.496 ± 0.538
4.309GluThr: 4.309 ± 0.443
3.902GluVal: 3.902 ± 0.454
1.382GluTrp: 1.382 ± 0.348
1.789GluTyr: 1.789 ± 0.323
0.0GluXaa: 0.0 ± 0.0
Phe
4.553PheAla: 4.553 ± 0.862
0.081PheCys: 0.081 ± 0.08
1.951PheAsp: 1.951 ± 0.391
1.138PheGlu: 1.138 ± 0.289
0.732PhePhe: 0.732 ± 0.299
3.089PheGly: 3.089 ± 0.445
0.407PheHis: 0.407 ± 0.173
1.301PheIle: 1.301 ± 0.363
1.707PheLys: 1.707 ± 0.346
2.52PheLeu: 2.52 ± 0.471
0.813PheMet: 0.813 ± 0.249
1.382PheAsn: 1.382 ± 0.299
1.382PhePro: 1.382 ± 0.429
0.569PheGln: 0.569 ± 0.183
1.951PheArg: 1.951 ± 0.348
2.033PheSer: 2.033 ± 0.438
2.358PheThr: 2.358 ± 0.505
2.52PheVal: 2.52 ± 0.512
0.407PheTrp: 0.407 ± 0.159
0.569PheTyr: 0.569 ± 0.18
0.0PheXaa: 0.0 ± 0.0
Gly
10.0GlyAla: 10.0 ± 1.009
0.976GlyCys: 0.976 ± 0.252
6.341GlyAsp: 6.341 ± 0.74
4.39GlyGlu: 4.39 ± 0.653
3.333GlyPhe: 3.333 ± 0.555
6.504GlyGly: 6.504 ± 0.874
1.138GlyHis: 1.138 ± 0.344
2.846GlyIle: 2.846 ± 0.593
4.797GlyLys: 4.797 ± 0.634
7.724GlyLeu: 7.724 ± 0.976
1.301GlyMet: 1.301 ± 0.273
3.252GlyAsn: 3.252 ± 0.422
4.228GlyPro: 4.228 ± 1.108
2.602GlyGln: 2.602 ± 0.487
7.154GlyArg: 7.154 ± 0.718
4.959GlySer: 4.959 ± 0.746
4.553GlyThr: 4.553 ± 0.865
5.854GlyVal: 5.854 ± 0.789
2.439GlyTrp: 2.439 ± 0.601
3.008GlyTyr: 3.008 ± 0.627
0.0GlyXaa: 0.0 ± 0.0
His
1.789HisAla: 1.789 ± 0.335
0.244HisCys: 0.244 ± 0.136
1.382HisAsp: 1.382 ± 0.323
1.138HisGlu: 1.138 ± 0.337
0.407HisPhe: 0.407 ± 0.155
1.545HisGly: 1.545 ± 0.391
0.163HisHis: 0.163 ± 0.128
0.813HisIle: 0.813 ± 0.261
0.65HisLys: 0.65 ± 0.259
1.626HisLeu: 1.626 ± 0.403
0.488HisMet: 0.488 ± 0.191
0.488HisAsn: 0.488 ± 0.231
1.463HisPro: 1.463 ± 0.35
0.081HisGln: 0.081 ± 0.089
1.22HisArg: 1.22 ± 0.28
1.138HisSer: 1.138 ± 0.309
0.976HisThr: 0.976 ± 0.265
1.626HisVal: 1.626 ± 0.31
0.488HisTrp: 0.488 ± 0.21
0.65HisTyr: 0.65 ± 0.302
0.0HisXaa: 0.0 ± 0.0
Ile
4.065IleAla: 4.065 ± 0.615
0.163IleCys: 0.163 ± 0.113
3.008IleAsp: 3.008 ± 0.342
2.846IleGlu: 2.846 ± 0.429
0.894IlePhe: 0.894 ± 0.244
2.927IleGly: 2.927 ± 0.442
1.301IleHis: 1.301 ± 0.558
1.545IleIle: 1.545 ± 0.383
2.439IleLys: 2.439 ± 0.55
1.789IleLeu: 1.789 ± 0.371
0.894IleMet: 0.894 ± 0.261
0.488IleAsn: 0.488 ± 0.208
2.358IlePro: 2.358 ± 0.458
1.057IleGln: 1.057 ± 0.283
2.276IleArg: 2.276 ± 0.442
1.382IleSer: 1.382 ± 0.23
2.52IleThr: 2.52 ± 0.401
2.439IleVal: 2.439 ± 0.405
0.244IleTrp: 0.244 ± 0.127
0.732IleTyr: 0.732 ± 0.239
0.0IleXaa: 0.0 ± 0.0
Lys
6.341LysAla: 6.341 ± 0.888
0.407LysCys: 0.407 ± 0.173
2.439LysAsp: 2.439 ± 0.513
1.87LysGlu: 1.87 ± 0.355
1.301LysPhe: 1.301 ± 0.39
3.415LysGly: 3.415 ± 0.632
1.301LysHis: 1.301 ± 0.317
1.057LysIle: 1.057 ± 0.273
1.951LysLys: 1.951 ± 0.442
3.008LysLeu: 3.008 ± 0.511
0.732LysMet: 0.732 ± 0.216
1.22LysAsn: 1.22 ± 0.405
3.415LysPro: 3.415 ± 0.584
0.488LysGln: 0.488 ± 0.195
4.228LysArg: 4.228 ± 0.575
2.52LysSer: 2.52 ± 0.544
2.602LysThr: 2.602 ± 0.563
3.821LysVal: 3.821 ± 0.58
0.65LysTrp: 0.65 ± 0.23
1.463LysTyr: 1.463 ± 0.405
0.0LysXaa: 0.0 ± 0.0
Leu
8.537LeuAla: 8.537 ± 0.949
0.813LeuCys: 0.813 ± 0.303
5.854LeuAsp: 5.854 ± 0.787
3.821LeuGlu: 3.821 ± 0.505
2.358LeuPhe: 2.358 ± 0.37
5.935LeuGly: 5.935 ± 0.622
1.22LeuHis: 1.22 ± 0.332
3.74LeuIle: 3.74 ± 0.504
3.415LeuLys: 3.415 ± 0.422
4.39LeuLeu: 4.39 ± 0.587
0.894LeuMet: 0.894 ± 0.226
3.252LeuAsn: 3.252 ± 0.426
5.447LeuPro: 5.447 ± 0.707
2.033LeuGln: 2.033 ± 0.39
6.992LeuArg: 6.992 ± 0.863
5.366LeuSer: 5.366 ± 0.611
6.341LeuThr: 6.341 ± 0.831
4.959LeuVal: 4.959 ± 0.667
1.301LeuTrp: 1.301 ± 0.265
1.707LeuTyr: 1.707 ± 0.387
0.0LeuXaa: 0.0 ± 0.0
Met
3.008MetAla: 3.008 ± 0.456
0.407MetCys: 0.407 ± 0.171
1.057MetAsp: 1.057 ± 0.258
0.569MetGlu: 0.569 ± 0.203
0.325MetPhe: 0.325 ± 0.127
2.033MetGly: 2.033 ± 0.448
0.488MetHis: 0.488 ± 0.204
0.894MetIle: 0.894 ± 0.271
1.301MetLys: 1.301 ± 0.324
1.626MetLeu: 1.626 ± 0.346
0.325MetMet: 0.325 ± 0.164
0.732MetAsn: 0.732 ± 0.221
0.894MetPro: 0.894 ± 0.259
0.488MetGln: 0.488 ± 0.233
1.626MetArg: 1.626 ± 0.35
1.626MetSer: 1.626 ± 0.325
1.626MetThr: 1.626 ± 0.409
1.301MetVal: 1.301 ± 0.323
0.325MetTrp: 0.325 ± 0.148
0.325MetTyr: 0.325 ± 0.183
0.0MetXaa: 0.0 ± 0.0
Asn
4.634AsnAla: 4.634 ± 0.585
0.163AsnCys: 0.163 ± 0.125
2.195AsnAsp: 2.195 ± 0.483
1.626AsnGlu: 1.626 ± 0.318
0.894AsnPhe: 0.894 ± 0.286
3.74AsnGly: 3.74 ± 0.502
0.65AsnHis: 0.65 ± 0.232
0.976AsnIle: 0.976 ± 0.299
1.382AsnLys: 1.382 ± 0.301
1.626AsnLeu: 1.626 ± 0.375
0.976AsnMet: 0.976 ± 0.235
0.488AsnAsn: 0.488 ± 0.187
2.358AsnPro: 2.358 ± 0.396
0.488AsnGln: 0.488 ± 0.245
1.22AsnArg: 1.22 ± 0.279
1.057AsnSer: 1.057 ± 0.328
1.301AsnThr: 1.301 ± 0.327
2.276AsnVal: 2.276 ± 0.485
0.488AsnTrp: 0.488 ± 0.276
0.407AsnTyr: 0.407 ± 0.169
0.0AsnXaa: 0.0 ± 0.0
Pro
5.366ProAla: 5.366 ± 0.732
0.488ProCys: 0.488 ± 0.19
3.74ProAsp: 3.74 ± 0.64
5.772ProGlu: 5.772 ± 0.971
1.87ProPhe: 1.87 ± 0.407
5.366ProGly: 5.366 ± 0.734
0.813ProHis: 0.813 ± 0.229
0.813ProIle: 0.813 ± 0.334
2.52ProLys: 2.52 ± 0.513
2.846ProLeu: 2.846 ± 0.6
1.057ProMet: 1.057 ± 0.288
2.358ProAsn: 2.358 ± 0.449
0.976ProPro: 0.976 ± 0.398
0.976ProGln: 0.976 ± 0.403
1.789ProArg: 1.789 ± 0.318
3.008ProSer: 3.008 ± 0.485
4.878ProThr: 4.878 ± 0.647
5.041ProVal: 5.041 ± 0.838
0.976ProTrp: 0.976 ± 0.302
1.463ProTyr: 1.463 ± 0.346
0.0ProXaa: 0.0 ± 0.0
Gln
2.602GlnAla: 2.602 ± 0.441
0.325GlnCys: 0.325 ± 0.136
0.813GlnAsp: 0.813 ± 0.222
0.976GlnGlu: 0.976 ± 0.315
0.976GlnPhe: 0.976 ± 0.239
2.683GlnGly: 2.683 ± 0.526
0.569GlnHis: 0.569 ± 0.189
0.813GlnIle: 0.813 ± 0.207
0.813GlnLys: 0.813 ± 0.285
1.626GlnLeu: 1.626 ± 0.417
0.65GlnMet: 0.65 ± 0.194
0.894GlnAsn: 0.894 ± 0.261
0.813GlnPro: 0.813 ± 0.265
0.244GlnGln: 0.244 ± 0.118
3.333GlnArg: 3.333 ± 0.542
1.057GlnSer: 1.057 ± 0.276
1.626GlnThr: 1.626 ± 0.394
1.87GlnVal: 1.87 ± 0.359
0.407GlnTrp: 0.407 ± 0.186
0.65GlnTyr: 0.65 ± 0.223
0.0GlnXaa: 0.0 ± 0.0
Arg
8.374ArgAla: 8.374 ± 0.943
0.813ArgCys: 0.813 ± 0.333
4.634ArgAsp: 4.634 ± 0.724
4.309ArgGlu: 4.309 ± 0.618
2.764ArgPhe: 2.764 ± 0.527
4.715ArgGly: 4.715 ± 0.719
1.382ArgHis: 1.382 ± 0.392
3.415ArgIle: 3.415 ± 0.649
3.659ArgLys: 3.659 ± 0.551
5.691ArgLeu: 5.691 ± 0.766
1.789ArgMet: 1.789 ± 0.491
1.626ArgAsn: 1.626 ± 0.419
2.195ArgPro: 2.195 ± 0.428
1.707ArgGln: 1.707 ± 0.368
4.797ArgArg: 4.797 ± 0.698
3.252ArgSer: 3.252 ± 0.662
3.902ArgThr: 3.902 ± 0.623
5.447ArgVal: 5.447 ± 0.583
1.707ArgTrp: 1.707 ± 0.366
2.52ArgTyr: 2.52 ± 0.517
0.0ArgXaa: 0.0 ± 0.0
Ser
8.211SerAla: 8.211 ± 0.936
0.732SerCys: 0.732 ± 0.245
3.902SerAsp: 3.902 ± 0.527
2.683SerGlu: 2.683 ± 0.427
1.951SerPhe: 1.951 ± 0.308
6.098SerGly: 6.098 ± 0.874
1.057SerHis: 1.057 ± 0.394
2.033SerIle: 2.033 ± 0.396
1.951SerLys: 1.951 ± 0.452
4.553SerLeu: 4.553 ± 0.632
1.382SerMet: 1.382 ± 0.276
0.894SerAsn: 0.894 ± 0.563
2.276SerPro: 2.276 ± 0.521
1.301SerGln: 1.301 ± 0.281
2.52SerArg: 2.52 ± 0.397
3.008SerSer: 3.008 ± 0.662
3.252SerThr: 3.252 ± 0.457
4.39SerVal: 4.39 ± 0.673
1.057SerTrp: 1.057 ± 0.336
1.22SerTyr: 1.22 ± 0.313
0.0SerXaa: 0.0 ± 0.0
Thr
7.154ThrAla: 7.154 ± 0.892
0.65ThrCys: 0.65 ± 0.28
5.041ThrAsp: 5.041 ± 0.896
3.74ThrGlu: 3.74 ± 0.552
2.52ThrPhe: 2.52 ± 0.482
6.748ThrGly: 6.748 ± 1.116
0.488ThrHis: 0.488 ± 0.218
2.114ThrIle: 2.114 ± 0.482
3.252ThrLys: 3.252 ± 0.692
7.073ThrLeu: 7.073 ± 0.842
1.301ThrMet: 1.301 ± 0.298
1.545ThrAsn: 1.545 ± 0.354
2.927ThrPro: 2.927 ± 0.494
1.463ThrGln: 1.463 ± 0.367
3.089ThrArg: 3.089 ± 0.577
3.252ThrSer: 3.252 ± 0.512
4.39ThrThr: 4.39 ± 0.706
6.179ThrVal: 6.179 ± 0.61
1.057ThrTrp: 1.057 ± 0.355
1.626ThrTyr: 1.626 ± 0.389
0.0ThrXaa: 0.0 ± 0.0
Val
10.244ValAla: 10.244 ± 1.139
0.894ValCys: 0.894 ± 0.361
3.496ValAsp: 3.496 ± 0.538
4.065ValGlu: 4.065 ± 0.597
2.358ValPhe: 2.358 ± 0.436
6.504ValGly: 6.504 ± 0.843
1.707ValHis: 1.707 ± 0.328
3.496ValIle: 3.496 ± 0.528
2.764ValLys: 2.764 ± 0.375
6.098ValLeu: 6.098 ± 0.761
1.545ValMet: 1.545 ± 0.386
2.114ValAsn: 2.114 ± 0.326
4.797ValPro: 4.797 ± 0.605
2.033ValGln: 2.033 ± 0.371
6.26ValArg: 6.26 ± 0.851
5.285ValSer: 5.285 ± 0.605
6.585ValThr: 6.585 ± 0.79
6.667ValVal: 6.667 ± 0.984
1.382ValTrp: 1.382 ± 0.394
2.52ValTyr: 2.52 ± 0.414
0.0ValXaa: 0.0 ± 0.0
Trp
2.276TrpAla: 2.276 ± 0.33
0.244TrpCys: 0.244 ± 0.134
0.976TrpAsp: 0.976 ± 0.298
0.976TrpGlu: 0.976 ± 0.277
0.894TrpPhe: 0.894 ± 0.283
1.301TrpGly: 1.301 ± 0.278
0.407TrpHis: 0.407 ± 0.2
0.488TrpIle: 0.488 ± 0.163
0.407TrpLys: 0.407 ± 0.162
1.951TrpLeu: 1.951 ± 0.467
0.163TrpMet: 0.163 ± 0.107
0.325TrpAsn: 0.325 ± 0.157
0.407TrpPro: 0.407 ± 0.203
1.22TrpGln: 1.22 ± 0.246
1.626TrpArg: 1.626 ± 0.439
1.138TrpSer: 1.138 ± 0.301
1.382TrpThr: 1.382 ± 0.254
1.707TrpVal: 1.707 ± 0.367
0.244TrpTrp: 0.244 ± 0.199
0.325TrpTyr: 0.325 ± 0.161
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.089TyrAla: 3.089 ± 0.537
0.407TyrCys: 0.407 ± 0.176
2.114TyrAsp: 2.114 ± 0.424
2.195TyrGlu: 2.195 ± 0.389
0.325TyrPhe: 0.325 ± 0.14
3.577TyrGly: 3.577 ± 0.535
0.244TyrHis: 0.244 ± 0.135
0.407TyrIle: 0.407 ± 0.2
0.732TyrLys: 0.732 ± 0.222
1.301TyrLeu: 1.301 ± 0.304
0.732TyrMet: 0.732 ± 0.195
0.732TyrAsn: 0.732 ± 0.221
1.545TyrPro: 1.545 ± 0.394
0.407TyrGln: 0.407 ± 0.155
2.764TyrArg: 2.764 ± 0.562
1.626TyrSer: 1.626 ± 0.388
1.22TyrThr: 1.22 ± 0.222
2.764TyrVal: 2.764 ± 0.409
0.081TyrTrp: 0.081 ± 0.08
0.407TyrTyr: 0.407 ± 0.177
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 56 proteins (12301 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski